1.Effects of benzyltetrahydropalmatine on atria and trachea in guinea pig
Chinese Pharmacological Bulletin 1987;0(01):-
Pinacidil caused a concentration -dependent decrease in the force of contraction,but, benzyltetrahydropalmatine ( BTHP ) increased the contractile force in isolated guinea pig left atria. Both BTHP and pinacidil suppressed the adrenaline- induced left atria auto-maticity.In isolated guinea pig trachealis,BTHP(5, 50 ?mol ?L-1) caused an apparent leftward shift of acetylcholine(Ach)or histamine dose-response curves, augmenting response to low Ach or histamine concentrations without or slightly change the tissue maximum response to the spasmogen. By contraries,Pinacidil(50 ? mol?L-1)produced a rightward shifts in the concentration - effect curve of Ach or Kis and depressed the foot of thedose-response curves for Ach or His without modifying the maximum response.In the presence of pinacidil BTHP did not modify the responses to either Ach or histamine. indeed,the specific antagonism between pinacidil and BTHP suggests that BTHP interact with the same or closely related sites of actions as the K channel blocker.
2.Clinic characteristics and prognosis on lacunar cerebra infarction of 112 cases
Chinese Journal of Primary Medicine and Pharmacy 2006;0(09):-
Objective To investigate clinic characteristics and prognosis of lacunar cerebra infarction.Methods 112 patients with lacunar cerebra infarction were selected and analyzed retrospectively.Results 40% of patients had slight symptom,symptom-free 25% of patients was observed by the infarct sign based on the CT brain scan or MRI,65% of patients was the frequency of cerebra infarction,infarct focus was 219 in the 112 cases,recrudescence in 76 patients including 35 patients(46%) with symptom of dementia,language barrier and diplegia.Conclusions Clinic symptom of patients with lacunar cerebra infarction is slight,whereas clinic characteristics are variegated.Recrudescence rate is highter in patients with a previous history of the lacunarr cerebra infraction,and prognosis is bad.With the number of infarct focus increasing,clinic symptom of cognize obstacle and other function abnormity become more serious.This results may help clinicians to assess prognosis more accurately.
3.The effect of hyperestrogenemia on myocardial contractility and relaxation in myocardial infarction observed in female rats
Chinese Journal of Pathophysiology 1989;0(06):-
The effect of hyperestrogenemia induced by intramuscular injectied estradiol (E_2) on myocardial contractility and relaxation was observed in female rats with myocardial infarction (MI). The results showed that: (1) In MI group, the plasma estradiol level increased slyghtly and then droped, while the level of estradiol increased markedly in rats treated by E_2 in MI (MIE_2) group. (2) Diameter of myofibril in MIE_2 grows more than that of control sham operation(CS) and MI (10.65?0.59 VS 7.65?0.40 and 10.15?0.54?m, P
6.Clinical evaluation of intraoperative cholangiography in minicholecystectomy
Feng LIU ; Zhiping ZHANG ; Jinyu LIU
Chinese Journal of Hepatobiliary Surgery 2009;15(5):350-351
Objective To determine the clinical value of intraoperative cholangiography in mini-cholecystectomy. Methods The clinical data of 506 patients receiving intraoperative cholangiography in minicholecystectomy from 1992 to 2006 in our hospital were analyzed retrospectively. Results Intr-aoperative cholangiography was successfully completed in 403 patients. The success rate was 97.4%.Stones in the bile duct in 49 patients,abnormal bite duet in 4 patients and bile duct injury in 4 patient were found. The cuts were extended in 16 patients. Conclusion Intraoperative cholangiography is of great value in inducing postoperative complications and improving the quality of MC. Therefore, it can be used to prevent residual stone after operation, avoid bile duct exploration or injury, decrease the chance of extending the cut and make sure of abnormal bile duct.
7.Biological characteristics of stromal cells derived from rat bone marrow in vitro
Zhiping YUAN ; Hongwei LIU ; Bin LIU
Journal of Practical Stomatology 2001;17(2):93-95
Objective:To study the biological characteristics and mineralization activity of strom cells (MSC) isolated from rat bone marrow.Methods: MSCs were isolated from rat bone marrow using gradient centrifuge (460 g,20 min) with 70% percoll and cultured with DMEM. The cells were then incubated with mineralization culture medium (MCM) in the presence of Dex (10~7 mol/L), β-GP (10 mmol/L) and AcA (50 g/L).Cell growth was studied with cell counting,alkaline phosphatase (ALP) expression with a kit and mineralization with a saturated solution of alizarin red S (pH 4.3) stainning.Results: The isolated cells from bone marrow of rat were epithelial-like. The doubling time (DT) of the cells in MCM or DMEM were 50.3 h and 66.1 h respectively. Mineralization was observed after the cells had been cultured in MCM for 28 days. bFGF and rhBMP-2 increased ALP expression of MSC in a dosage dependent manner. Conclusion:In vitro cultured MSCs may be induced to differentiate towards osteoblasts.
8.Biological characteristics of stromal cells derived from rat bone marrow in vitro
Zhiping YUAN ; Hongwei LIU ; Bin LIU
Journal of Practical Stomatology 1996;0(02):-
砄bjective:To study the biological characteristics and mineralization activity of strom cells (MSC) isolated from rat bone marrow.Methods: MSCs were isolated from rat bone marrow using gradient centrifuge (460 g,20 min) with 70% percoll and cultured with DMEM. The cells were then incubated with mineralization culture medium (MCM) in the presence of Dex (10~7 mol/L), ? GP (10 mmol/L) and AcA (50 g/L).Cell growth was studied with cell counting,alkaline phosphatase (ALP) expression with a kit and mineralization with a saturated solution of alizarin red S (pH 4.3) stainning.Results: The isolated cells from bone marrow of rat were epithelial like. The doubling time (DT) of the cells in MCM or DMEM were 50.3 h and 66.1 h respectively. Mineralization was observed after the cells had been cultured in MCM for 28 days. bFGF and rhBMP 2 increased ALP expression of MSC in a dosage dependent manner. Conclusion: In vitro cultured MSCs may be induced to differentiate towards osteoblasts.
9.Effects of rosiglitazone on the proliferation of and expressions of β-catenin and cyclin D1 in HaCaT cells
Ni FAN ; Zhiping WEI ; Yanqun LIU
Chinese Journal of Dermatology 2012;45(9):644-646
Objective To estimate the effects ot rosiglitazone on cultured HaCaT human keratinocytes and their possible mechanism.Methods HaCaT cells were cultured and treated with different concentrations ( 10,20,40,80 μ mol/L) of rosiglitazone or solvent for 24,48,72 and 96 hours,respectively.Cell proliferation was detected with methyl thiazolyl tetrazolium (MTT) assay.Western blot was performed to measure the protein expression of β-catenin and cyclin D1.Results Compared with the solvent-treated cells,the proliferation of HaCaT cells was significantly inhibited by 18.9%,23.7%,35.1% and 44.6% (all P< 0.05) after treatment with rosiglitazone of 10,20,40 and 80 μmol/L,respectively,for 48 hours.The expressions of β-catenin and cyclin D 1 were significantly lower in rosiglitazone-treated HaCaT cells than in solvent-treated cells (all P < 0.05).Conclusion Rosiglitazone could inhibit the proliferation of HaCaT cells,likely by downregulating the expressions of β-catenin and cyclin D 1.
10.In vitro induction of apoptosis of a cutaneous squamous cell carcinoma cell line, Colo-16 cells, by sirolimus
Yuan LI ; Zhiping WEI ; Yanqun LIU
Chinese Journal of Dermatology 2008;41(10):670-673
Objective To investigate the in vitro effect of sirolimus on the apoptosis of a cutaneous squamous cell carcinoma cell line, Colo-16 cells. Methods Cultured Colo-16 cells were treated with different concentrations (50, 100, 150, 200 nmol/L) of sirolimus for various durations ( 12, 24, 48, 72 hours). Subse-quently, cell proliferation was detected by MTT assay, and cell apoptosis by Annexin V-FITC and PI double staining. Morphological changes of the cells were observed with Hoechst 33258 fluorescent staining. Total RNA was extracted from Colo-16 cells treated with sirolimus for 48 hours, and subjected to reverse tran-scription (RT)-PCR for the detection of mRNA expression of B cell lymphoma/leukmia-2 (Bcl-2) and Bcl-2-associated X Protein (Bax). Results Sirolimus inhibited the proliferation of Colo-16 cells in a time-and dose-dependent fashion. The early apoptosis rate was 7.26%±0.26%, 8.34% ±0.19%, 9.86%±0.14%, 11.92% ±0.15% in Colo-16 cells treated with sirolimus of 50, 100, 150, and 200 nmol/L, respectively, signifi-candy higher than that in untreated cells (1.53%±0.09%, P < 0.05); a positive correlation was observed between the apoptosis rate and concentrations of sirolimus (r = 0.955, P = 0.000). Typical morphological changes of apoptosis, such as chromatin condensation and margination as well as nuclear fragmentation were observed by fluorescence staining. After treatment with sirolimus for 48 hours, a significant decrease was observed in the mRNA expression of Bcl-2, while an increase in that of Bax was noticed. Conclusion Sirolimus could induce Colo-16 cells apoptosis in vitro, which may be associated with the modulation of expression of apoptosis-regnlating genes, such as Bcl-2 and Bax.