We selected several platiniferous and non-platiniferous chemotherapy strategies of typical in the article to analyze patients with non-small cell lung cancer can tolerate non-platiniferous chemotherapy strategies, side effects are smaller than p]atiniferous chemotherapy strategies.

Objective:To observe the effect of CTLA-4Ig on preventing acute rejection and the role of costimulatory molecule B7-1 and B7-2 via the model of orthotopic liver transplantation(ROLT)in rats.Methods:Build the model of ROLT(DA-Lewis).All rats were divided into 2 groups randomly.Group A was control group.Group B was injected with CTLA4-Ig into abdominal cavity after 48 hours of operation.Animals were sacrificed on the day 3,5,7,10 after ROLT to detect the changes of serum biochemistry(ALT,TBIL and DBIL).Liver grafts were collected for measuring the expression of B7-1 and B7-2 mRNA by RT-PCR.Results:1)Compared with the control group,the expression of B7-1 and B7-2 mRNA increased significantly(P

The molecular and cellular mechanisms of bone metastasis in prostate cancer remain unclear. Current researches focus on chemotaxis of tumor cells in bone metastasis, interactions between tumor cells and bone micro-environment as well as the vicious circle among tumor cells, osteoclasts, osteoblasts and bone matrix.

Objective:To explore the mediating effect of belief in a just world and self-esteem on relationship between childhood abuse and subjective well-being in college students.Methods:Totally 929 college students[314 males and 615 females,aged 17 to 25 year,mean age (20 ± 3) year] from 4 universities of Harbin were assessed.The Childhood Trauma Questionnaire (CTQ-SF) was used to assess childhood abuse,the Satisfaction with Life Scale (SWLS) and the Affect Balance Scale (ABS) was used to assess subjective well-being,the Believe in a Just World Scale (BJW) was used to assess belief in a just word,and the Self-Esteem Scale (SES) was used to assess self-esteem.The Bootstrap was used to test the mediating effect of belief in a just world and self-esteem on relationship between childhood abuse and subjective well-being.Results:General belief in a just world,personal belief in a just world,self-esteem played an independent mediating effect respectively between childhood abuse and life satisfaction.The confidence interval from Bootstrap output were 95％ (-0.026--0.001),95％ (-0.141--0.058) and 95％ (-0.235--0.122).Personal belief in a just world and general belief in a just world played chain mediating effect between childhood abuse and life satisfaction (-0.059--0.004).Self-esteem played an independent mediating effect between childhood abuse and emotional balance (-0.308--0.181).Personal belief in a just world and general belief in a just world played chain mediating effect between childhood abuse and emotional balance (-0.046--0.001).Conclusion:Childhood abuse and subjective well-being of individuals,belief in a just world and self-esteem are related.Belief in a just world and self-esteem play mediating effect between childhood abuse and subjective well-being.

There are a variety of serological changes in the blood circulation of patients with lupus nephritis,including complement,autoantibodies,immunoglobulin and cytokines and so on.These substances not only involved in the development of the disease leads to renal pathological damage,but also can be used as a biomarker to reflect changes in disease.In this paper,the detection of serum markers in lupus nephritis diagnosis,determine the pathological classification,disease activity,evaluation of therapeutic effect and prognosis were reviewed,in order to provide scientific basis for the early diagnosis and treatment of lupus nephritis by monitoring the changes of serum indicators.

Fatty liver disease is caused by abnormal accumulation of lipids within hepatocytes. According to traditional Chinese medicine (TCM) theory, lipids belong to the category of essence obtained from cereals and the normal distribution of essence relies on the function of spleen yang. When spleen yang is injured, the normal distribution of essence (lipids) will be affected, leading to formation of phlegm retention in the liver. That is the TCM pathogenesis of fatty liver disease. Hence the treatment of fatty liver disease should be concentrated on warming yang to activate qi. With such a treatment, the normal distribution of essence will be restored, essence will be distributed, and phlegm will be dissipated.

Objective To observe the concentration change of 0.5% peracetic acid used as disinfectant in the indoor air. Methods KC-6D gas-sampler and ion chromatograph were employed and sampling interval time was 10-15 min, 7 samples were collected each time in both of the condition of closed and ventilated, the same experiment was repeated for 3 times. Results As the time went on, the concentration change of 0.5% peracetic acid in the indoor air increased from 6.24 mg/m3 to 20.72 mg/m3 in the condition of closed and decreased in the condition of ventilated from 6.92 mg/m3 to 3.36 mg/m3. Conclusion In a closed room of 20 m3, using 500 ml 0.5% peracetic acid, after 30 minutes of disinfection followed by 60 minutes of ventilation, the concentration of peracetic acid will be about 3.36 mg/m3 in the air of the room.

Objective:To investigate the expression of costimulatory molecule B7-1 and B7-2 by warm ischemia/reperfusion(I/R)injury in rat liver and the meaning of immunology.Methods:Male Wistar rats were randomly divided into 3 groups:A(ischemia 30 mins,referfusion 24h),B(ischemia 60 mins,referfusion 24h),C(sham operation group).A model of hepatic warm I/R in rats was established according to the method of Ohmorid.The expression of B7-1 and B7-2 mRNA was analyzed by real-time reverse transcription polymerase chain reaction(RT-PCR).Results:Compared with the sham operation group,the expression of B7-1 and B7-2 mRNA increased significantly in the rats undergoing I/R procedure(P

Objective To explore the possibility of gelatin sponge as supporter of central nervous tissue engineering. Methods Primary NSCs were isolated from forebrain of neonatal Sprague Dawley rats and cuhured in serum-free medium for long-term survival in vitro. Neural stem cells were divided into absorbable gelatin sponge group and control group.Observe their morphology and proliferation.Immunofluorescence technique were used to test the results of differentiation of two groups of neural stem cells. Resultes NSCs in absorbable gelatin sponge group and control group survived and there was no conspicuous change in shape and quality. The rates of survival cell were 91.6％ and 92.8％ respectively, which was no significant difference between them.NSCs could adherented to the surface of the gelatin sponge and well-grown.After induced differentiation,NSCs started to shrink and stretch,axons grown and connected with each other,till form network structure.The expression of neuroglia cell marker GFAP and neuron cell marker NSE could be detected by immunofluorescence assay. Couclusion Neural stem cells and gelatin sponge can mixed and cultivating together in vitro.NSCs is no conspicuous change.It is suggested that absorbable gelatin sponge can serve as the carrier of the tissue engineering of central nervous system.

Objective To discuss the survivorship of rat mesenchymal stem cells (rMSCs) and the expression of human brain-derived neurotrophic factor (hBNDF) protein after transplantation of the hBDNF-modified rMSCs (hBDNF-rMSCs) to the adult rats with spinal cord injury (SCI) and discuss the effect of hBDNF-rMSCs on the apoptosis of rat neural cells. Methods A total of 240 adult male Sprague-Dawley rats were randomly divided into sham operation group,SCI group,hBDNF-rMSCs transplantation group and empty vector-rMSCs transplantation group,with60 rats in each group.The SCI model was established by using the modified Allen technique.At day 7 after modeling,an equal volume of hBDNFrMSCs suspension,empty vector-rMSCs suspension and phosphate buffered saline (PBS) were injected through the L4,5 subarachnoid space into the hBDNF-rMSCs group,empty vector-rMSCs group and SCI group,respectively.Then,the injured spinal cord tissues were obtained from each group at days 1,2,3,7 and 14 after transplantation to observe the viability and distribution of rMSCs with enhanced green fluorescent protein gene by fluorescent microscope,measure the expression of hBDNF protein by Western blot and detect the apoptosis of neural cells by TdT-mediated dUTP nick end labeling (TUNEL). Results Both hBDNF-rMSCs and empty vector-rMSCs groups showed green fluorescence expression of rMSCs.The hBDNF protein expression was observed in hBDNF-rMSCs group and changed with time,ie,the expression was detected at day 2 after transplantation,reached the highest level at day 7 and then decreased gradually.Among the hBDNF-rMSCs,empty vector-rMSCs and SCI groups,the number of TUNEL positive cells was the least in hBDNF-rMSCs group,followed by the empty vector-rMSCs group and the number was relatively more in SCI group at days 2,3,7 and 14 after transplantation,with significant differences among groups (P ＜ 0.05). Conclusions Transplantation of the hBDNF-modified rMSCs through subarachnoid approach are able to survive and assemble at the injured spinal cord area and express hBDNF protein.The hBDNF-modified rMSCs can inhibit the apoptosis of neural cells after SCI.