Enteropathy-Associated T-Cell Lymphoma ; classification ; pathology ; Gastrointestinal Tract ; pathology ; Humans

Enteritis ; diagnosis ; Humans ; Ulcer ; diagnosis

As a fundamental medical course,operative surgery is featured by basic skill acquirement and plenty of practice.The teaching method for operative surgery is different compared with that for other medical course.Personal digital assistant (PDA) was adopted to facilitate the teaching of the course and this was an innovation.By taking advantage of its multiple electronic functions and various application softwares,PDA was applied to arrange class schedule,show teaching demonstration,make action analysis and give feedback and incentives of both students and teachers.In this way,PDA was proved to be an effective educational media for teaching of operative surgery.

With the development of wireless networks and mobile intelligent terminal the new media technology is profoundly changing people's lifestyle and study mode. We applied new media technologies to the teaching practice of eight-year medical program to stimulate students' learning en-thusiasm and productivity, which greatly expanded the knowledge of students, enrich teaching methods, and achieved good teaching effect.

Objective The protective effect of hydrogen sulfide (H2 S) against myocardial ischemia/reperfusion ( IR) injury via anti-apoptotic signaling is well established , but the underlying mechanism remains unclear .This study was to investigate whether H 2 S could protect cardiomyocytes from endoplasmic reticulum stress ( ERS)-mediated apoptosis in hypoxia/reoxygenation ( HR) injury by regulating the expression of microRNA-455 ( miR-455 ) . Methods Cardiomyocytes from neonatal SD rats were primarily cultured and the model of HR injury was established .The cardiomyocytes were divided into a control group (normally cultured for 27 hours), an HR group (subjected to HR injury), and an H2S protection group (pretreated with the precursor of H2S NaHS at 40 μmol/L at 30 min before HR treatment followed by the same procedure as in the HR group ) .The cell viability was monitored by MTT , the release of lactate de-hydrogenase ( LDH) in the culture supernatant measured by full-automatic chemical analysis , and the apoptosis rate of the cardiomyo-cytes detected by flow cytometry .The mRNA and protein expressions of Grp 78 and caspase-12 were determined by real-time RT-PCR and Western bot .To verify whether miR-455 was involved in the ERS-mediated apoptosis of the cardiomyocytes , the cells were subjec-ted to HR after transfected with miR-455 mimic or anti-miR-455 oligonucleotide (AMO) for 24 hours, followed by detection of the ex-pressions of Grp78 and caspase-12. Results After HR injury, the H2 S protection group showed an enhanced viability of the cardio-myocytes in comparison with the control group ([67.02 ±6.90] vs [29.27 ±5.66] %), an decreased LDH release ([91.33 ± 10.63] vs [168.17 ±15.38] U/L), and a reduced rate of cell apoptosis ([13.98 ±1.90] vs [24.31 ±2.79] %).H2 S pretreat-ment significantly downregulated the mRNA and protein expressions of Grp 78 and caspase-12 (1.66 ±0.39 vs 2.56 ±0.34;1.75 ± 0.32 vs 2.54 ±0.48;2.01 ±0.45 vs 3.26 ±0.34;1.85 ±0.52 vs 3.21 ±0.84, P<0.05).The mRNA and protein expressions of Grp78 and caspase-12 were evidently increased after transfection with miR-455 mimic (3.56 ±0.37 vs 1.00 ±0.00;3.61 ±0.41 vs 1.00 ±0.00;2.87 ±0.38 vs 1.00 ±0.00;2.98 ±0.49 vs 1.00 ±0.00), but remarkably decreased after transfection with miR-455 AMO (0.62 ±0.16 vs 1.00 ±0.00;0.65 ±0.13 vs 1.00 ±0.00;0.54 ±0.13 vs 1.00 ±0.00;0.62 ±0.16 vs 1.00 ±0.00, P<0.05). Conclusion H2S could protect cardiomyocytes from HR injury by regulating the expression of miR-455 and reducing ERS-mediated cell apoptosis .

OBJECTIVETo compare the biological characteristics of colorectal cancer associated fibroblasts (CAFs) with normal fibroblasts (NFs).

METHODSCAFs and NFs were isolated from fresh specimens of colorectal cancer and their paired normal colon tissue and cultured by tissue explant method. Light microscopy, quantitative polymerase chain reaction (qPCR), Western blot, immunofluorescence microscopy, electron microscopy and flow cytometry were used to identify isolated fibroblasts and to explore their characteristics of activation and growth.

RESULTSPrimary colorectal CAFs and NFs were isolated and cultured successfully. NFs showed spindled morphology and were arranged in interlacing or spiral bundles. CAFs were polygonal or spindle, but were fatter than NFs. They were distributed randomly and arranged irregularly, and had obvious actin expression. CAFs and NFs both expressed fibronectin, but not E-cadherin, CD31 and caldesmon. qPCR showed that CAFs expressed more fibroblast activation protein (FAP) and less fibroblast specific protein 1 (FSP1) than that of NFs. There was no difference in the expression of α-SMA between NFs and CAFs by Western blot. α-SMA was bundled in parallel to the long axis of the cell by immunofluorescence. By electron microscopy, CAFs but not NFs showed dense myofilament that was arranged regularly. Flow cytometry showed that the percentage of S- and G2-phase in CAFs were significantly lower than that in NFs. mRNA expression of transforming growth factor β1, stromal derived factor 1 (SDF-1) and platelet derived growth factor (PDGF)-D in CAFs were lower while that for PDGFC was higher than that in NFs. That indicated the proliferation of CAFs was inhibited and the secretion of some cytokines was different when compared with NFs.

CONCLUSIONSCAFs show differences with NFs in morphology, characteristics of activation and secretion of some cytokines. The proliferation of CAFs is down regulated as compared with NFs.

Blotting, Western ; Cell Movement ; Cell Proliferation ; Colorectal Neoplasms ; pathology ; Fibroblasts ; cytology ; Flow Cytometry ; Fluorescent Antibody Technique ; Humans ; Primary Cell Culture ; Tumor Cells, Cultured

Objective To compare the biological characteristics of colorectal cancer associated fibroblasts ( CAFs) with normal fibroblasts ( NFs).Methods CAFs and NFs were isolated from fresh specimens of colorectal cancer and their paired normal colon tissue and cultured by tissue explant method . Light microscopy, quantitative polymerase chain reaction ( qPCR), Western blot, immunofluorescence microscopy , electron microscopy and flow cytometry were used to identify isolated fibroblasts and to explore their characteristics of activation and growth .Results Primary colorectal CAFs and NFs were isolated and cultured successfully .NFs showed spindled morphology and were arranged in interlacing or spiral bundles . CAFs were polygonal or spindle , but were fatter than NFs .They were distributed randomly and arranged irregularly , and had obvious actin expression . CAFs and NFs both expressed fibronectin , but not E-cadherin, CD31 and caldesmon.qPCR showed that CAFs expressed more fibroblast activation protein (FAP) and less fibroblast specific protein 1 ( FSP1) than that of NFs.There was no difference in the expression of α-SMA between NFs and CAFs by Western blot .α-SMA was bundled in parallel to the long axis of the cell by immunofluorescence . By electron microscopy , CAFs but not NFs showed dense myofilament that was arranged regularly .Flow cytometry showed that the percentage of S-and G2-phase in CAFs were significantly lower than that in NFs .mRNA expression of transforming growth factor β1, stromal derived factor 1(SDF-1) and platelet derived growth factor (PDGF)-D in CAFs were lower while that for PDGFC was higher than that in NFs .That indicated the proliferation of CAFs was inhibited and the secretion of some cytokines was different when compared with NFs .Conclusions CAFs show differences with NFs in morphology , characteristics of activation and secretion of some cytokines .The proliferation of CAFs is down regulated as compared with NFs .

Pulmonary blast injury has become the main type of trauma in modern warfare, characterized by externally mild injuries but internally severe injuries, rapid disease progression, and a high rate of early death. The injury is complicated in clinical practice, often with multiple and compound injuries. Currently, there is a lack of effective protective materials, accurate injury detection instrument and portable monitoring and transportation equipment, standardized clinical treatment guidelines in various medical centers, and evidence-based guidelines at home and abroad, resulting in a high mortality in clinlcal practice. Therefore, the Trauma Branch of Chinese Medical Association and the Editorial Committee of Chinese Journal of Trauma organized military and civilian experts in related fields such as thoracic surgery and traumatic surgery to jointly develop the Clinical treatment guideline for pulmonary blast injury ( version 2023) by combining evidence for effectiveness and clinical first-line treatment experience. This guideline provided 16 recommended opinions surrounding definition, characteristics, pre-hospital diagnosis and treatment, and in-hospital treatment of pulmonary blast injury, hoping to provide a basis for the clinical treatment in hospitals at different levels.