Objective To explore the protein expressions of matrix metalloproteinase-9 (MMP-9) and tissue inhibitor of metalloproteinase-1 (TIMP-1), and to investigate their relationships between their serum concentration before operation and the infiltration and metastasis of thyroid carcinoma. Methods The protein expressions of MMP-9 and TIMP-1 in 32 cases of thyroid carcinomas, 23 cases of adjacent tissues and 30 cases of benign hyperplastic lesions were measured by using immunohistochemistry. The preoperative serum concentrations of MMP-9 and TIMP-1 in 21 cases of thyroid carcinomas and 19 cases of benign hyperplastic lesions were determined by enzyme-linked immunosorbent assay. Results The positive expression rates of MMP-9 and TIMP-1 in tumor tissues were significantly higher (75.0%,56.3%)than those in adjacent tissues and benign hyperplastic lesions (30.4%, 21.7%; 26.7%, 23.3%) P

Objective To observe the difference clinical therapeutic efficacy of the Chinese herbal medicine and western medicine on sick sinus syndrome patients.Method 171 patients with sick sinus syndrome were randomly divided into treatment group and control group.90 patients in the treatment group were given Qiangxin Fumai Mixture while the other 81 patients in the control group were given atropine.The therapeutic course was four weeks in each group.The changes of clinical manifestations,electrocardiogram(24 h Holter monitoring ECG) and left ventricular ejection fraction(LVEF) were compared between two groups.Result Clinical therapeutic efficacy:There was significant difference between two groups(P

BACKGROUND:The effects of artificial joint replacement, osteotomy and reconstruction in the treatment of Crown Ⅳ-type congenital dislocation of hip in adult are not very satisfied. OBJECTIVE:To evaluate the effect of artificial total hip arthroplasty and hip-self procedure in the treatment of Crown Ⅳ-type congenital dislocation of hip in adult patients. METHODS:Twenty-three adult patients with Crown Ⅳ-type congenital dislocation of hip were treated with artificial total hip arthroplasty and hip-self procedure. There were 2 males and 21 females with an average age of (24.26±3.56) years ranging 20 to 35 years. The effect was evaluated according to the Harrris evaluation standard, and the statistical analysis was performed. RESULTS AND CONCLUSION:Al of the patients were fol owed-up for 12-60 months, averaged of (26.60±13.16) months. Statistical comparison with the SPSS 19.0 system showed there was significant difference of the Harrris scores between preoperation and postoperation period (P<0.05). The artificial total hip arthroplasty and hip-self procedure can be used to reconstruct the normal function of hip joint, relieve pain and increase the joint stability, which is considered as the best method for the treatment of Crown Ⅳ-type congenital dislocation of hip in adult.

To study the determination method of cholesterol in human serum by Coulometric titration. METHODS: The titration was carried out in an electrolytic solution containing 1 mol.L-1 potassium bromide and glacial acetic acid (1∶3). Bromine was electrogenerated at the anode and cholesterol reacted with bromine with a value of reactional electrons (n) as 2. RESULTS: The ranges of recovery for 0.115 mg to 0.459 mg of cholesterol were from 93.9% to 105%, and the average recovery was 100.3%. The relative standard deviation in all applied analysis was less than 1.5%. The accuracy of the method was also assessed in comparison with the results obtained by the enzymatic method used clinically. CONCLUSION: The method proposed is simple, rapid and accurate, and is suitable for the determination of cholesterol in human serum samples.

Objective To realize compliance actuality, discuss effect factor and institute correspond countermeasure for diabete integration treatment. Method Investigate 251 patients with diabete who did colligate treatment and community with correlation factor. Results The rate of mostly complication of diabetes was higher (66.53%), and the rate of colligate treatment was lower (only 29.08%). The compliance of treatment had relation with the rate of knowing diabete, symptom and complication, economy factors, medicine insurance, and lever of doctor closely. Conclusion We should intensify diabetes knowledge education, increase medical treatment specialty level, associate with community intervene, supervise and direction, establish consummate medical treatment insurance system to increase colligate treatment of diabetes sufferer.

Dengue virus (DENV) nonstructural protein 1 (NS1) is a highly conserved 46-kDa protein that contains 2 glycosylation sites (Asn-130 and Asn-207) and 12 conserved cysteine (Cys) residues. Here, we performed site-directed mutagenesis to generate systematic mutants of viral strain TSV01. The results of the subsequent analysis showed that an alanine substitution at the second N-linked glycan Asn-207 in NS1 delayed viral RNA synthesis, reduced virus plaque size, and weakened the cytopathic effect. Three mutants at Cys sites (Cys-4, Cys-55, Cys-291) and a C-terminal deletion (ΔC) mutant signiifcantly impaired RNA synthesis, and consequently abolished viral growth, whereas alanine mutations at Asn-130 and Glu-173 resulted in phenotypes that were similar to the wild-type (WT) virus. Further analysis showed that the Asn-207 mutation slightly delayed viral replication. These results suggest that the three conserved disulifde bonds and the second N-linked glycan in NS1 are required for DENV-2 replication.

Objective To investigate the expression of tumor necrosis factor-?(TNF-?) and intracellular adhesion molecular-1(ICAM-1) in brain tissue after experimental intracerebral hemorrhage in rats.Methods Experimental intracerebral hemorrhage model was maded by injection autologous blood using stereotactic method. The expression of TNF-? and ICAM-1 in cerebral tissues at different time were detected by using immunohistochemitry techniques.Results TNF-? expression raised obviously at 6 h and peaked at 48 h.ICAM-1 expression raised obviously at 12 h and peaked at 72 h.There was obvious difference between each intracerebral hemorrhage group and controls(all P

Aim To explore the effect and mechanism of atorvastatin on lipopolysacchairde (LPS) inducing the expression of inhibitor of nuclear factor ?B? (I?B?) in human vascular endotheliar cells. Methods The human vascular endotheliar cell line ECV304 was cultured and divided into five groups as control group, LPS group, and low, moderate or high does atorvastatin groups. After incubated with different densities atorvastatin, the three atorvastatin groups and LPS group were stimulated with LPS 30min. Then the activation of I?B? was observed with immnofluorescence. The proteins expressions of I?B? and phosphorylated I?B? were detected with western blot. The ex-pression of I?B? mRNA was examined with reversetranscription-polymerase chain reaction. Results Atorvastatin could inhibit the translocation of p65 to the nucleus and reduce the phosphorylation and degradation of I?B? in a dose-dependent manner. The high density atorvastatin could increase the expression of I?B? mRNA. Conclusion The atorvastatin can inhibit the activation of nuclear factor ?B by regulating the expression and degradation of I?B?.

Objective To evaluate the cost differences in two groups of BPH patients undergone suprapubic transvesical prostatectomy or TULVP. Methods A comparison of the cost-effectiveness was undertaken,including days of catheter indwelling and hospitaliztion,follow-up results,cost financial expense as related to different operative procedures. Results In TULVP group,the days of catheter indwelling and hospitalization were significantly shortened and the total expenditure was decreased (P

In view of the deficiencies of both Qi and Yin in most cases of this tumor, Jinfukang oral solution (JFK). a remedy mainly for benefiting Qi and nourishing -yin. was formulated. 96 cases were treated with one case of CR. 8 cases PR, 52 NC. the rate of PR + NC being 63. 5%. Another 52 cases were treated with chemotherapy +JFK. with 11 cases of PR, 26 cases NC, the rate of PR + NC being 71. 2%. While 25 cases were treated by chemotherapy alone, the cases of PR was 4; NC 26. the rate of PR + NC being 60. 0%, This demonstrated that the effect of JFK and JFK + chemotherapy were all superior to effect of chemotherapy alone. For 1 - and 2 - year survival rates, the JFK group was 67. 3% and 66. 7% respectively, whereas that of JFK + chemotherapy. 67. 3% and 66. 7%. For the chemotherapy group , 1 - year survival rate was 40. 3%, with no survivals within 2 years. For symptom improvement, body weight gaining, and health condition (KPS evaluation) after treatment, the JFK and JFK + chemotherapy groups were, again, superior to the chemotherapy group. The Immunity indices and blood picture in JFK group after treatment were markedly improved as compared with those before treatment, but, those in the chemotherapy group were decreased, while those of the JFK + chemotherapy group remained unchanged.