Objective To study the effect of rosiglitazone on pathologic changes of ? cell ultrastructure in insulin-resistant (IR) rats. Methods IR rat model was established in 6-8-week-old SD rats by high-glucose feeding (70% calories from glucose) for 6 weeks. The IR rats was then divided into IR group (n=10) and rosiglitazone (RSG) group (n=10). Rats in RSG group were treated with RSG 10 ?mol?kg -1?d -1 for 6 weeks by gavage. Ultrastructure of ? cells was observed with transmission electron microscope. Results Systolic blood pressure and serum levels of triglyceride, free fatty acids and insulin in RSG group were lower than those in IR group (all P

Objective To study the effect of rosiglitazone on pathologic changes in the ultrastructure of aortic endothelia in insulin-resistant (IR) rats. Methods IR rat model was prepared in 6-8-week-old SD rats by high-glucose chaw (70% calories from glucose) for 6 weeks. IR rat model was divided into IR group (n=10) and rosiglitazone (RSG) group (n=10). Rats in RSG group were given rosiglitazone 10 ?mol?kg -1?d -1 (16:00) by gavage for 6 weeks. Ultrastructure of aortic endothelia was observed with transmission electron microscope. Results Systolic blood pressure and serum levels of triglyceride, free fatty acid and insulin in RSG group were lower than those in IR group (both P

To study the effect of melatonin on pathologic changes in beta cells ultrastructure of insulin-resistant(IR) rats, insulin-resistant rat model(n=20) was reproduced in 6-8 week old Sprague-Dawley(SD) rats by feeding with high-glucose diet (70% calories from glucose)for 6 weeks. The IR model rats were divided into an IR group(n=10) and a melatonin(MEL) group(n=10). Rats in the MEL group were given melatonin 10mg?kg -1?d -1(4PM) introgastrically for 6 weeks. Ultrastructure of beta cells was observed with a transmission electron microscope(TEM). The results showed:systolic blood pressure(SBP) and serum triglyceride(TG), low-density lipoprotein(LDL-c),insulin(Ins), malondialdehyde(MDA) in MEL group were lower than in IR group (all P

The aim of this study was to observe the changes of melatonin and its receptor in ischemic cerebral apoplexy(ICA) rats and the effect of melatonin pretreatment on The ICA rats. The ICA rat model was reproduced by using Pulsinelli′s method. Serum corticosterone and melatonin concentration were determined in each group. The radioactivity of melatonin receptor in the brain and peripheral leucocytes was deteced by -Mel radioligand binding assay. Bmax and Kd of melatonin receptor were calculated. The results indieated serum corticosterone in apoplexy group was higher than in sham operation group (P

this paper analyzed the imbalanced distributions of information,also known as information excess and information desert for the 21st century university students,and a series of related straits initiated by the two phenomena.The education of life ethics view for university students in the 21st century is proposed to be strengthened as a countermeasure.

Objective: To study the preventive effect of microecological preparations on enteral nutrition related diarrhea in elderly patients.Methods:Sixty-two eldealy patients with enteral nutrition were divided into treatment group(n=30) and control group(n=32) at random.All patients were fed with Nutrtion Fibre.Treatment group was given with microecological preparations.The incidence rate of diarrhea,and the time to reach standard amount were recorded.Results: The incidence rate of diarrhea in the treatment group was 13.33%,and the incidence rate of diarrhea in the control group was 37.50%(P

Objective To explore the effect and molecular mechanism of N-myc downstream regulated gene 1 (NDRG1) on transforming growth factor-beta (TGF-β)-induced epithelial-mesenchymal transition (EMT) in human lung cancer cells.Methods Lung cancer A549 cells were transfected with NDRG1 overexpressed vector,and then treated with 5 μg/L TGF-β1.The abilities of invasion were detected by Transwell assay.The expressions of NDRG1 mRNA and protein were analyzed by teal-time reverse transcription polymerase chain reaction (RT-PCR) were examined with Western blot.The expressions of EMT-associated markers E-cadherin and Vimentin,and EMT-associated signaling molecules Snail,AKT and Smad were detected with Western blot.Results We found that TGF-β1 treatment could induce morphological alteration of A549 cells from epithelial morphology to mesenchymal morphology.TGF-β1 significantly increased the migration of A549 cells,and increased the expression of mesenchymal maker vimentin and decreased epithelial marker E-cadherin.More importantly,overexpression of NDRG1 significandy reversed the effects of TGF-β1 on A549 cells.Moreover,NDRG1 significandy decreased the levels of phospho-AKT,and suppressed the expression of EMT-related transcription factor Snail.Conclusions NDRG1 could reverse the effects of TGF-β1 on EMT in A549 cells,by which mechanism is related to reduction of the expressions of phospho-AKT and Snail.

Objective To study the effect of melatonin on ultrastructural changes of aorta endothelium of insulin-resistant (IR) rats. Methods Insulin-resistant rat model was established with SD rats (n=20) aged 6-8 weeks by high-glucose diet (70% calories from glucose) for 6 weeks. All the IR rats were divided into 2 groups: with melatonin (MEL group, n=10) and without melatonin (control group, n=10). Rats in MEL group were filled with melatonin (10mg? kg -1?d -1) for 6 weeks. The ultrastructure of aorta endothelium was observed with transmission electron microscope (TEM). Results Systolic blood pressure (SBP), serum triglyceride (TG), low-density lipoprotein (LDL-c), insulin (Ins), and malondialdehyde (MDA) declined while the Serum high-density lipoprotein(HDL-c), superoxide dismutase (SOD) and insulin sensitive index (ISI) elevated in the rats of MEL group than that in control group (P

To investigate the melatonin receptor(MR) gene and protein expression in human HUT78 cell line, total RNA of HUT78 cells was isolated by single-step method of acid guanidinium-thiocyanate-phenol-chloform, and mt 1 and MT 2 mRNA were determined by reverse transcription-polymerase chain reaction(RT-PCR). The Envision method was applied to immunohistochemistry to identify and localize mt 1 and MT 2 protein. The mt 1 cDNA fragment of the expected size of 370bp was determined, but the MT 2 cDNA fragment of the expected size of 320bp was not determinable by RT-PCR. Sequencing result indicated that the positive product coincided with the cDNA of human mt 1. The mt 1 protein was observed by immunohistochemistry. These buffy positive granules were scattered with some areas stronger than the others, and were primarily located in cytoplasm and membrane, with rare location in nucleus; meanwhile the MT 2 protein was not observed. These results demonstrated the mt 1 mRNA and protein expression in HUT78 cell line. It is indicated that melatonin has directly immune-regulative effects on T lymphocyte, The changes of MR in physiological and pathological stages need to be investigated.

Objective To explore the effects of rosiglitazone on the expression of TNF-? in visceral adipose tissue of insulin-resistant rats induced by high-glucose diet. Methods Sprague-Dawley (SD) rats were fed with high-glucose diet for 6 months to reproduce the non-obesity insulin-resistant rat model (abbreviated as IR rats). The model rats were then randomized into insulin resistan (IR) group and rosiglitazone (ROS) group (10 each). Ten rats fed with normal diet were enrolled as controls (CN group). Rats in ROS group were administered with 10?mol/(kg?d) of rosiglitazone for 6 weeks from the 7th week of experiment,and those in CN and IR groups were administered with same amount of distilled water. At the 6th and 12th week,the systolic pressure,body weight,and biochemical parameters (fasting and 2-hour post-glucose load plasma glucose,insulin,free fatty acid,triglyceride,high density lipoprotein cholesterol) were measured,and insulin resistance index (HOMA-IR) was calculated. At the end of the 12th week,the visceral adipose tissue was collected to calculate the ratio of visceral fat to body weight (VF/BW),and to determine the protein and mRNA expressions of tumor necrosis factor-? (TNF-?) with inmmunohistochemical assay and RT-PCR. Results As compared with CN group,the systolic pressure,HOMA-IR,plasma insulin,triglyceride and free fatty acid increased,whereas high density lipoprotein cholesterol decreased (P0.05). Inmmunohistochemical examination showed positive expression of TNF-? in intercellular substance,but not in visceral adipocytes. The expressions of TNF-? protein and mRNA in visceral adipose tissue were dramatically higher in IR group than in CN and ROS groups (P0.05). Conclusion Rosiglitazone may significantly abate insulin resistance,reduce the abnormality of plasma lipid and blood pressure,and inhibit the over expression of TNF-? in visceral adipose tissue in the non-obesity insulin-resistant rats induced by high-glucose diet.