Lipid-adjust therapy is one of basic management of atherosclerosis diseases with approaches like medication,therapeutics life style(TLC),dialysis regimen,surgery operation and gene therapy etc.Along with lipidlowering medication,TLC methods should be used to primary and secondary prevention of coronary artery diseases,by which lipid levels returns to target normal ranges as a result of reducing uptake of cholesterol or saturation fatty foods,improving physical activities,as well as weight control.

Objective To approach the clinical applied value of MSCT coronary angiography in detecting coronary artery disease. Methods 150 patients underwent MSCT coronary angiography, of them, 100 patients were also examined by coronary arteriography. The ability in showing coronary arteriae with MSCT was evaluated comparatively with coronary arteriography. Results The success-ful rate in showing coronary arteriae by MSCT was 95.3%,the excellent rate of images was 82.970%. There was negative correlation between image quality and heart rate(r=0.938). And the accurate rate in evaluating the narrowing of coronary arteriae was 91.2% with MSCT. There was no significant difference in detecting the narrowing of coronary arteriae with MSCT and coronary angiography (P>0.05). Conclusion By controlling the patients' heart rate(below 70/min) and optimizing the technical methods,MACT coro-nary angiography can detect all of the 1～3 branch of coronary artery. MSCT has some particular superiority in diagnosing coronary artery disease and evaluating the effects of coronary artery bypass graft.

Objective To study MRI manifestation of lymphoma of bone marrow. Methods Ten patients(8 males and 2 females) with lymphoma of bone were performed MRI. Two cases were primary (1 HD and 1 NHL) and 8 were secondary NHL. Results (1)Location: All cases involved axial bones; (2)Number: 9 cases involved multiple bones, only 1 primary NHL involved solitary bone; (3)Signal Intensity: The marrow infiltration were showed low and high signal intensity on T 1WI and T 2WI respectively; (4)Shape: 9 cases were showed multiple focal features, another one showed diffuse changes; (5)Pathological Fracture: 3 cases associated with pathological fractures of vertebrae. Conclusion The sensitivity of MRI was excellent for detecting marrow infiltration of lymphoma, but the specificity was poor.

Objective:To study the effect and underlying mechanism of rosmarinic acid in the apoptosis of HepG2 cells. Meth-ods:An MTT method was used to determine the inhibitory effect on the growth of HepG2 cells treated with rosmarinic acid for 48 hours. Flow cytometry was used to detect the cell apoptosis after treated with rosmarinic acid at different concentrations for 24 h and 48 h. Western Blotting was used to detect the expression of P53 and c-Myc protein after treated with rosmarinic acid for 48 h. Results:When HepG2 cells were treated with rosmarinic acid at the concentrations of 12. 50, 25. 00, 50. 00 and 100. 00 μg·ml-1 for 48 h, the cell growth was inhibited and the half-inhibitory concentration (IC50) was 43. 48μg·ml-1. By comparing HepG2 cells treated with rosma-rinic acid at different concentrations for 24 h and 48 h, rosmarinic acid had the effect on the early apoptosis of HepG2 cells after the 48-hour treatment. When treated with rosmarinic acid at the different concentrations for 48 h, the expression of c-Myc decreased with the concentration increase of rosmarinic acid, and the expression of P53 increased with the concentration increase of rosmarinic acid. Con-clusion:The inhibitory effect of rosmarinic acid on the proliferation of HepG2 cells is in a dose-dependent manner, and it can promote the apoptosis of HepG2 cells through the activation of P53 and the cleavage of proto-oncogene c-Myc protein.

Objective To observe the therapeutic efficacy and evaluate the health economics of early rehabilitation intervention applied to patients with cerebral hemorrhage in stroke unit. Methods A total of 131 cerebral hemorrhage patients were randomly assigned to the stroke unit group (n=62) and the control group (n=69). The patients in the stroke unit group received rehabilitation training at a very early stage, while there were no rehabilitation training program and no professional treatment team for patients in the control group. Results of the national institute health stroke scale (NIHSS), activities of daily living (ADL) Barthel index were compared between the two groups. Infection rate, mortality, cost of hospitalization and length of stay were also compared. Results There were better therapeutic effects as revealed by NIHSS (4.3±3.5 vs. 7. 9±5.0, t=-3. 211, P<0.05), ADL Barthel index score (85.9±29.6 vs. 67.1±37.1, t=3.194, P<0.05), lower incidence of infection in lung (8.06% vs. 15.94%, χ~2 =3.901, P<0.05) and in urinary tract (6.45% vs. 11.59%, χ~2 =4. 138, P<0. 05), lower mortality (4.84% vs. 7.25%, χ~2=4. 351, P< 0.05), lower cost of hospitalization[￥ (17506. 90±954.10 ) vs. ￥(21096.49±923.46), t=-20. 786, P<0.01)] and shorter length of stay[(20. 47±7. 03)d vs. (31. 42±8.14)d, t=-8.196, P< 0.01)] in the stroke unit group compared to the control group. Conclusions Early rehabilitation intervention by stroke unit is advantageous to patients with cerebral hemorrhage.

Objective To study the mental experience of nurses from outpatient department in dealing with patient's complaints.Method Using convenient sampling method,15 nurses from 5 sections of the outpatient department were chosen as interviewees,with the interviews on their mental experience in handling the complaints from outpatients.Result The issues from the interviews included:part of the patients were unable to complain accurately,part of the nurses were ineffective and therefore inactive in handling the complaints;some of the nurses were poor at employing communication skills and some others were incapable of specifying the complaints or asking for assistance.Conclusions Complaint handling is part of the nurses' job in the outpatient service.The nurses should take the initiative to improve their ability in complaint handling,so as to handle the complaints in a positive and effective way and improve patient's satisfaction.

Objective To investigate the alteration of left atrial(LA) electromechanical characteristics of patients with hypertensivon and patients with hypertension complicated with paroxysmal atrial fibrillation(PAF). Methods By use of two-dimentional echocardiography and Doppler and tissue Doppler imaging(TDI) techniques,we mensurated and calculated the indexes representing LA structure and systolic and diastolic function and electromechanical characteristics in 30 nornal subjects(group1),25 patients with hypertension lasting less than ten years(group2),24 patients with hypertension lasting more than ten years(group3) and patients with hypertension complicated PAF(group4). Indexes representing LA electromechanical characteristics consisted of the difference in time intervals between the QRS onset and the tricuspid A wave,and the QRS onset and the mitral A wave (DQAT),electromechanical time (P-A),electromechanical time dispersion(P-Ad),the maximum P wave duration,the minimum P wave duration,and P wave dispersion (Pd=Pmax-Pmin) calculated from 12-lead surface electrocardiograms. Results The P-A3 in group 4 was much longer than that in group 1,group 2 and group 3. The P-Ad2 in group 4 was much longer than that in group 1,group 2 and group 3.Conclusion There are prolonged P-A3 and P-Ad2 in patients with hypertension complicated PAF. Some indexes,such as P-A3 and P-Ad2,perhaps can predict the onset of PAF in patients with essential hypertension better.

Objective:To analyze genetic diversity of Fluconazole(FLZ)-resistant and -susceptible Candida albicans(C. albicans) strains isolated from patients with Sjgren's Syndrome (SS). Methods:30 C.albicans strains were isolated from the patients with SS and identified using standard criteria. Microdilution method was performed to determine the minimum inhibitory concentrations (MICs) of C.albicans to FLZ. A composite genotype was generated for each strain through random amplification of polymorphic DNA (RAPD) using three different primers, RSD10, RSD11 and RSD12. Results:The DNA fingerprinting profiles indicated genetic diversity amongst both the FLZ-resistant as well as -susceptible isolates, and no specific features emerged distinguishing the drug-resistant and -susceptible groups. Conclusion:These observations cast doubt on the theory of a clonal origin of FLZ-resistant C. albicans isolates. The emergence of FLZ resistance in SS patients may be associated with continuous exposure to FLZ.

To inquire into the influence on effect, safety and receiving rate of thrombolysis in acute myocardial infarction (AMI) after extending its selective indications. We observed the main indication changes and effect of 201 cases of AMI receiving thrombolytic therapy who from 108 patients with AMI. Results: (1)On basis of the thrombolytic scheme recommended by the committee of Chinese Journal of Cardiology in 1991,further extend the time of onset to medication from ≤6 hr. to ≤12 hour,age from ≤65 yr,to all yr, unlimited. The ratio of cases receiving thrombolysis/all AMI is 49. 3%, more than before extending indications (28. 1% ,P0. 05) between group 6- 12 hr,medication (n- 32) and ≤6 hr's (n= 169) ,but its mortality in both groups above is significantly lower than group non-thrombolysis (18. 4%,both P

A reversed phase high performance liquid chromatographic ( RP-HPLC ) method was proposed by using C18 column tandem crown ether chiral column for simultaneous separation of three kinds of aromatic amino acid enantiomers. The chromatographic conditions, including mobile phase proportion, pH, column temperature and flow rate, were optimized. The experimental results showed that the three aromatic amino acid enantiomers could be successfully separated under the optimal conditions such as perchloric acid solution-acetonitrile ( 86:14, V/V, pH = 2 ) as mobile phase, column temperature of 20℃ and flow rate of 0. 4 mL/min. The separation under four HPLC column connection modes was further investigated. The results revealed that different kinds of amino acids could be separated on C18 column, but not for amino acid enantiomers; amino acid enantiomers could be separated on crown ether chiral column, but the chromatographic peaks of each amino acid enantiomers overlapped seriously; baseline separation of three amino acid enantiomers could be obtained under the connection of two columns, but the separation under different column connection sequence almost had no difference except the peak shape.