Objective To explore the optimum dose of multislice spiral CT scan in the check of patients with chronic obstructive pulmonary disease(COPD).Methods 90 COPD patients were divided into the conventional dose group and low dose group,the quality of conventional-dose and low-dose scan was analyzed respectively.Results The two scanning methods enable images satisfactory requirements in the 90 cases,and low-dose CT radiation dose was significantly reduced(t=5.01,6.82,both P＜0.05).Conclusion For COPD patients using multi-slice spiral CT scan,low-dose scanning can not only meet the requirements of clinical diagnosis,but also significantly reduce the patient radiation dose.

Objective To investigate the CR low dose radiography to COPD in patients with pseudo normal phenomenon.Methods 160 cases of COPD diagnosed,stratified random dividing method according to the pulmonary function test values were divided into A,B,C and D group.Each group had 32 cases,at the same time,the increase in lung function in patients with mild to moderate pulmonary emphysema in 32 cases was E group,wich was treated with normal dose as a control group,in the normal dose and low dose CR photography failed to clear the diagnosis of emphysema,causing false normalization,statistical the case number,and verify the pulmonary function classification criteria,identify false normalization of lesions in such a case,the general rule to judge the feasibility analysis and prevention.Results There was no significant chifference among 5 groups of pseudo normal comparison (x2 =4.56,P ＞0.05).Conclusion Chronic obstructive pulmonary disease with low dose CR photography on severe and extremely severe patients is deteded by emphysema detection with rate high.Moderate,mild COPD detection rate is extremely low.

AIM To study the antiperoxide damage of injectio tanshin during dissolving thrombus to cure the acute myocardial infarction(AMI). METHODS AMI model formatted by electricity to irritate the left ventricular branch of coronary artery of open-chest rabbit,and observation aspects including electrocardiogram(ECG),cardiac output and contractility,serumal enzymatic activities and biochemistry and pathology of lipid peroxide extent of myocardial infarction zone. RESULTS By urinate kinase(UK) combining with injection tanshin,abnormal changes of ECG, descending of cardiac contractiles and cardiac output,going up of serum cardiac damage enzyme(CPK-MB and LDH),and the malondialdehyde(MDA)of serum and myocardial infarction zone could be remarkably reduced or avoided during UK dissolving the thrombus to cure AMI rabbits. CONCLUSION Injection tanshin can clearly reduce or avoid lipid peroxidation damage during UK dissolving the thrombus to cure the acute myocardial infarction.

Objective To investigate the effects of nimesulide, a selective cyclooxygenase-2 (COX-2) inhibitor, on human cholangiocarcinoma QBC939 cell line in vitro. Methods The effects of nimesulide on QBC939 cells were observed with the following techniques: the influence of nimesulide on the proliferation of QBC939 cells was determined by MTT assay; the apoptosis of QBC939 cells was viewed and measured by transmission electron microscopy and flow cytometry, respectively; the expressions of proliferation cell nuclear antigen (PCNA) and COX-2 of cholangiocarcinoma cells were detected by immunocytochemistry. Results Nimesulide inhibited the expressions of PCNA and COX-2 and the proliferation of cholangiocarcinoma QBC939 cells, whose effects intensified as the dose increased and time elongated. Flow cytometry showed that the apoptotic rates of QBC939 cells increased significantly as the dose of nimesulide increased. The typical morphologic features of apoptosis were also observed by transmission electron microscopy. Conclusion Nimesulide significantly inhibits the proliferation of QBC939 cells in vitro by inducting cell apoptosis, which may be associated with the downregulation of COX-2 expression, and it also presents the features of dose and time dependents.

Objective To investigate the effect of a selective inhibitor of COX-2 nimesulide on growth and apoptosis of human cholangiocarcinoma cell line QBC939 in vitro. Methods MTT assay was used to determine the influence of nimesulide on the proliferation of QBC939 cells, apoptosis of QBC939 was measured by transmission electron microscopy and flow cytometry.Expression of apoptosis related genes mRNA and bcl-2 ,bax, survivin were detected by RT-PCR and immunocytochemistry. Results Nimesulide effects a dose-dependent and time-dependent growth inhibition on QBC939 cells. High concentration of nimesulide (200 ?mol/L) not only inhibits the growth of QBC939 cells but also induces apoptosis cell nuclear condensation and apoptotic bodies were seen by transmission electron microscopy. Immunocytochemistry and RT-PCR shows upregulation of bax and down regulation of bcl-2 and survivin. Conclusion Nimesulide significantly inhibits the proliferation of QBC939 in vitro by induction of apoptosis in a dose- and time- dependent manner.

BACKGROUND:Stem cel transplantation is a new method for blinding eye disease. But there is a lack of research about the protective effect of retinal stem cel transplantation on retinal ganglion cel s in glaucoma. OBJECTIVE:To explore the protective effect of retinal stem cel transplantation on retinal ganglion cel s of rats with glaucoma. METHODS:Forty-five Sprague-Dawley rats were randomly divided into three groups (n=15 per group) including control, model and retinal stem cel transplantation groups. Rat models of glaucoma were prepared in the latter two groups, and at 7 days after modeling, rats in the three groups were given intravitreal injection of 1 mL retinal stem cel s (5x106 cel s), the same amount of PBS, and no treatment, respectively. Subsequently, relative indicators were detected at 2 weeks after transplantation. RESULTS AND CONCLUSION:The expressions of brain-derived neurotrophic factor and insulin-like growth factor I protein as wel as the number of retinal ganglion cel s were the highest in the control group, fol owed by the retinal stem cel transplantation group model group, and the lowest in the model group (P<0.05). The number of apoptotic retinal ganglion cel s in model group was significantly higher than that of control group (P<0.05), and which in the retinal stem cel transplantation group was significantly lower than that in the model group (P<0.05), but higher than that in the control group (P<0.05). These results suggest that retinal stem cel transplantation for rat glaucoma can exert a protective effect on retinal ganglion cel s.

Objective To evaluate the effect of ischemic preconditioning(IPC) on ischemia-reperfusion-injury of myocardium and phosphorylated Akt(p-Akt) during the disease course of diabetes in rats.Methods The rat model of diabetes mellitus was reprodused by streptozocin(40mg/kg,i.v.).Myocardial ischemia was then created by temporary ligation of a branch of the left anterior descending(LAD) coronary artery after 2 and 9 weeks,respectively.Thirty six SD rats were randomly divided into six groups(6 each):non-diabetic IPC(NDMIP) group,non-diabetic I/R(NDMIR) group,2-week diabetic IPC(2DMIP) group,2-week diabetic I/R(2DMIR) group,9-week diabetic IPC(9DMIP) group and 9-week diabetic I/R(9DMIR) group.Ischemic/reperfusion was induced by temporary occlusion of LAD coronary artery.After the experiment,creatine kinase-MB(CK-MB) isoenzyme and myocardial infarct size were measured,and the expression of p-Akt was detected by Western-blotting,the arrhythmia score was then evaluated.Results CK-MB level in NDMIP group was significantly lower than that in NDMIR group and 9DMIP group(P0.05);the arrhythmia score in 9DMIP group was significantly higher than that in 2DMIP group(P0.05);the expression of p-Akt in 9DMIP group was significantly lower than that in 2DMIP group(P0.05);the myocardial infarct size in 9DMIP group was larger than that in 2DMIP group.Conclusions IPC can provide significant microvascular protection against prolonged ischemia/reperfusion in acute diabetic rats,but not in chronic diabetic rats.The attenuation of myocardial protection by IPC may be associated with a decrease in p-Akt activation.

AIM For the evaluation of algorithms for circulator drug delivery, a new simulator was developed. METHODS The Clement Yu's non-linear canine circulatory system model was modified for the use of the part of digital dog of the simulator. And the other parts of the simulator were built by the means of object-oriented designing (OOD) with Visual C++ language. The proportion of mean arterial blood pressure in the range of?0.667 kPa of the object value was contracted to T_ MAP , and the average value of integral of excess of the range above was contract to S_ MAP . The difference of T_ MAP and S_ MAP of SPN MMAC(Multiple Model Adaptive Control) controlled hypotension and SPN constant-rate-controlled hypotension had been studied in vivo. This process was repeated in simulation system. The difference of simulator and animal experiments was studied while they were evaluating the SNP MMAC algorithms for controlled hypotension. RESULTS The mean arterial blood pressure graphs of the simulator and animal experiments acted similarly when they evaluated the SNP MMAC algorithms. The graph of mean arterial blood pressure of simulation was similar to that of the animal experiment when the MMAC was evaluated. CONCLUSION The new simulator can evaluate algorithms for circulator drug delivery and it's helpful to the building of such systems.-

AIM: To study the effect of puerarin (Pue) on related cytokines in myocardial hypertrophy induced by isoproterenol (Iso) in rats. METHODS: 40 SD rats were randomly divided into 4 treatments groups: control group, Iso group, Iso+Pue group (Pue group) and Iso+Captoril (Cap) group (Cap group), with 10 rats each group. Ratio of ventricle weight to heart weight, cardiomyocyte diameter, plasma and myocardial tissue NO, ET and Ang Ⅱ levels were measured. RESULTS: Compared with the control group, Iso did induce significant changes in cardiac hypertrophy (P