1.Study of relationship between cervical cancerous and cervical intraepithelial neoplasia and HPV infection
Chinese Journal of Primary Medicine and Pharmacy 2006;0(08):-
Objective To determine the relationship between cervical cancerous and precancerous lesions and human papilloma virus(HPV) infection.Methods 158 female patients with abnormal pap smears underwent colpo- scopically directed biopsies and hybrid captureⅡtest.Results HPV infection was distributed in 8.7 % of cervitis, in 34.7 % of low-grade squamous intraepithelial lesion(LSIL) ,in 68.9 % of high-grade squamous intraepithelial le- sion(HSIL) and in 83.3 % of cervical cancer.Conclusion HPV infection was correlated with cervical(cancerous and intraepithelial neoplasia) lesions and increased wi.th the serious degrees of lesions.Hybrid captureⅡtest can work ef- ficiently as a triage of patients with ASCUS,LSIL and HSIL before colposcopically directed biopsies.
2.Determination of the Content of Berberine Hydrochloride in Weibining Tablets by RP-HPLC
Yuan YAN ; Dan GUO ; Zhiliang CHEN
China Pharmacy 2001;0(07):-
OBJECTIVE:To determine the content of berberine hydrochloride in Weibining tablets METHODS:RP-HPLC was used with Nova-Pak C18 column as stationary phase The mobile phase was 0 033mol/L postassium dihydrogen phosphate-acetonitrile(60∶40) The UV detection wavelength was 265nm RESULTS:There was a good liner relationship within the concentration range of 0 32~1 6?g/ml of berberine hydrochloride(r=0 9 999) The average recovery was 99 07%(n=5,RSD=0 85%) CONCLUSION:This method is simple,fast and accurate
3.A literature analysis of power frequency electric field testing data.
Suli ZHANG ; Zehua GUO ; Xintian YU ; Yan DING ; Zhiliang ZHU
Chinese Journal of Industrial Hygiene and Occupational Diseases 2015;33(6):459-460
OBJECTIVETo analyze the literature on power frequency electric field testing data and to propose views and suggestions for current testing.
METHODSThe literature on power frequency electric field testing data published in the previous years was searched to identify 306 articles involving 193 valid testing data. Mann-Whitney test and Wilcoxon W test were used for analyzing the testing data.
RESULTSThe classification of data was carried out according to one quarter of occupational exposure limit (1.25 kV/m), one half of the exposure limit (2.5 kV/m), and the exposure limit (5 kV/m). The structure of testing data showed a significant difference between the non-power facility group and the power facility group (P<0.05).
CONCLUSIONAs occupational hazard factors, the radiation exposure from power frequency electric field is extensive. However, the power frequency electric field testing data in actual workplaces except high-voltage power facilities are far less than the occupational exposure limit with little harmfulness. There is a phenomenon of excessive testing at present.
Electricity ; Electromagnetic Fields ; Humans ; Occupational Exposure ; standards ; Workplace
4.Application of selective intra-arterial brain hypothermia in acute ischemic stroke
Zhiliang GUO ; Shuhong YU ; Xin CHEN ; Wusheng ZHU
International Journal of Cerebrovascular Diseases 2015;(9):699-703
Therapeutic hypothermia has been shown to improve neurological outcomes after global ischaemia/hypoxia in patients who have had cardiac arrest. Therapeutic hypothermia is one of the most extensively studied and influential therapeutic strategies of acute ischemic stroke. Previous studies of therapeutic hypothermia mainly focused on whole-body cooling. However, delayed induction and systemic complications have limited the clinical application of whole-body cooling. As a selective cerebral hypothermia, the selective intra-arterial brain hypothermia treatment has the characteristics of rapid and even induction of hypothermia, and less affecting the core body temperature. Therefore, it has become a promising treatment modality. This article reviews the application of selective intra-arterial brain hypothermia in acute ischemic stroke and its advantages and limitations in order to provide reference for further experimental studies and future clinical trials.
5.Signal transduction pathway for cardiac myocyte contraction induced by Substance K in rats
Min CHENG ; Liping YANG ; Zhenyu WEI ; Yuan GAO ; Zhiliang GUO ;
Journal of Third Military Medical University 2003;0(09):-
Objective To study the signal transduction pathway for myocyte contraction induced by Substance K (SK) in rats. Methods The fluorescent Ca 2+ indicator Fluo 3AM was used to quantitate the calcium signal directly in the primary cultured myocardial cells. Changes of free Ca 2+ concentration in cardiac myocytes were detected by flow cytometry. The phospholipase C (PLC) inhibitor, neomycin and IP3 receptor antagonist, heparin, were used to block signal system of phosphates Ca 2+ in order to investigate whether they took part in the SK induced changes of [Ca 2+ ]i in myocytes. Results SK(1.78?10 -5 mol/L) elevated [Ca 2+ ]i in myocytes. The effects could be blocked by neomycin and heparin. Conclusion SK can elevate [Ca 2+ ]i in myocyte, which may be mediated by a signal system of phosphates Ca 2+ .
6.Determination of Tinidazole in Yintai Cleaning Solution by HPLC
Dan GUO ; Yuan YAN ; Pingbo MA ; Zhiliang CHEN
China Pharmacy 1991;0(04):-
OBJECTIVE:To establish a HPLC method for determining the content of tinidazole in Yintal cleaning solu?tion.METHODS:Nova-Pak C 18 column was used.The mobile phase was methanol-water-glacial acetic acid(22∶78∶0.1). Internal standard was metronidazole.The UV detection wavelength was310nm.RESULTS:There was a good linerarity within the concentration range of20~100?g/ml of tinidazole in Yintai solution(r=0.9999).The average recovery was101.2%(n=5,RSD=1.16%).CONCLUSION:This method is simple,fast and accurate.
7.Genetic polymorphisms of interleukin-28B are associated with spontaneous clearance of hepatitis C virus infection
Junqiang XIE ; Xiaoyan GUO ; Xinhua LI ; Jie LUO ; Zhiliang GAO ; Zhixin ZHAO
Chinese Journal of Infectious Diseases 2012;30(9):538-541
Objective To explore the association between interleukin (IL)-28B variation and spontaneous clearance of hepatitis C virus (HCV) infection.MethodsA total of 280 HCV infected patients including 200 chronic hepatitis C (CHC) patients and 80 spontaneous clearance patients of HCV infection were enrolled in the study.The rs8099917 single nucleotide polymorphyism (SNP) of IL-28B gene was detected and the association between IL-28B variation and spontaneous clearance of HCV infection was analyzed.The data were compared using chi square test.ResultsThe genotype frequencies of TT and non TT (TG and GG) of IL-28B rs8099917 between CHC patients and spontaneous clearance patients were not significantly different ( x2 =15.874,P < 0.01 ). The prevalence of TT genotype in the spontaneous clearance group was 86.2%,which was higher than that in CHC group (62.0%).T and G allele frequencies in CHC group were 78.0% and 22.0%,respectively,and those in spontaneous clearance group were 92.5% and 7.5%,respectively,which indicated that T allele was dominant in spontaneous clearance patients. The allele frequency of rs8099917 was statistically different between the two groups (P<0.01).The spontaneous HCV clear rate in rs8099917 TT patients was 2.84 folds of non-TT patients,while the risk of chronicity of non-TT (TG+GG) patients was 1.36 folds of TT patients.Conclusion The IL-28B (rs8099917) TT genotype is associated with spontaneous clearance of HCV infection,which can be an important factor to predict the spontaneous clearance of HCV.
8.Effect of coenzyme complex on cardiac and renal functions of patients with type 2 cardiorenal syndrome
Jia WANG ; Ruofeng CHEN ; Jianhao GUO ; Liyao ZHOU ; Zhujun TAN ; Zhiliang LI
The Journal of Practical Medicine 2015;31(16):2648-2650
Objective To investigate the effect of coenzyme complex on the cardiac and renal functions of patients with type 2 cardiorenal syndrome. Methods Sixty-two patients with type 2 cardiorenal syndrome were enrolled in from June 2013 to December 2014 in Zhujiang Hospital , Southern Medical University. These patients divided were into routine group (n = 31) and coenzyme complex (n = 31). The therapy scheme of coenzyme complex group was on the basis of routine group with coenzyme complex intravenous drip , 400 U/day for 2 weeks. The cardiac function was determined by New York Heart Function Assessment, and the cardiac ultrasound, the levels of BNP. The renal function was determined by serum creatinine and urine volume. Results Compared with routine group, the rates of NYHA Ⅲ and Ⅳ were reduced, the LVEF levels were increased and the levels of BNP were increased (P < 0.05). The serum creatinine was decreased and urine volume were increased in the coenzyme complex group (P<0.05). Conclusion Coenzyme complex could improve the cardiac and renal functions of the patients with type 2 cardiorenal syndrome.
9.En-bloc kidney transplantation from small infant donors to adult recipients: a report of 22 cases
Lan ZHU ; Bin LIU ; Rula SA ; Zhiliang GUO ; Huibo SHI ; Fanjun ZENG ; Gang CHEN
Chinese Journal of Organ Transplantation 2021;42(1):3-7
Objective:To summarize the clinical outcomes of en-bloc kidney transplantation from small infant donors to adult recipients at a single center.Methods:A total of 22 en-bloc transplantations from pediatric donors to adult recipients were performed from July 2013 to October 2017 in Institute of Organ Transplantation Affiliated Tongji Hospital Tongji Medical College Huazhong University of Science. Clinical data were retrospectively analyzed. The average age of 22 donors was (2.9±1.7) months with an average weight of (4.9±1.4) kg and 15 of them were aged under 3 months. The average weight of 22 adult recipients was (46.3±5.6) kg and most recipients were female. The causes of early graft failure and recipient death were examined during follow-ups. The recipients with functioning grafts post-transplantation were divided into single kidney survival group and en-bloc kidney survival group based upon the occurrence of unilateral vascular thrombosis. Medium/long-term renal graft function was compared between two groups.Results:Early graft failure occurred in 4 recipients. The causes were bilateral renal vascular thrombosis ( n=2), renal rupture ( n=1) and multiple organ failure followed by death ( n=1). Eighteen recipients were discharged after a recovery of renal graft function. One case had a removal of bilateral renal grafts due to new-onset graft tumor and another two died from interstitial pneumonia and complicate systematic disorder respectively during follow-ups. Among the remaining 15 recipients, 10 achieved bilateral renal survival (median follow-up: 59 months) and 5 unilateral renal survival (median follow-up: 48 months). The average eGFR of bilateral renal survival group was significantly higher than that of unilateral renal survival group at Year 1 post-transplantation (95±27 vs 61±24 ml/min/1.73 m 2, P<0.05) while the gap narrowed at Year 3 and there was no statistical significance (95±21 vs 69±31 ml/min/1.73 m 2, P=0.12). Conclusions:Although en-bloc kidney transplantation from infant donors can expand organ donor pool, there is a higher risk of early graft failure and unilateral renal vascular thrombosis. Nevertheless, satisfactory renal transplant outcomes may be achieved in patients with unilateral renal graft survival.
10.Vascular endothelial factor C and E-cadherin relationship with the invasion and metastasis of human gastric cancer
Weiming LIU ; Xuefeng LI ; Jian GUO ; Zhiliang SHI ; Guoqiang ZHOU ; Jian PENG ; Chuandan WAN
Clinical Medicine of China 2016;32(6):502-505
Objective To investigate the expression of vascular endothelial factor C(VEGF-C) and E-cadherin in gastric cancer and explore the relationship between expression of VEGF-C and E-cadherin and the pathogenesis of gastric cancer.Methods Real-time quantitative reverse ranscriptase-polymerase chain rection was applied on 40 cases of gastric cancer and corresponding adjacent noncancerous tissues,in order to detect mRNA expression of VEGF-C and E-cadherin gene.VEGF-C and E-cadherin protein expression in gastric cancer and adjacent normal gastric mucosa were detected by immunohistochemistry.Statistical analysis was carried out to analyze the correlation among VEGF-C,E-cadherin and protein expression with various clinical parameters in these gastric cancer patients.Results The expression of VEGF-C protein in 40 cases of gastric cancer's cancer tissues was 0.461±0.012,significantly higher than that in the adjacent normal tissues(0.036+0.023;t=1.101,P<0.05),but E-cadherin expression was significantly lower than that of the adjacent normal tissues (0.079±0.002 vs.0.321±0.005;t=1.844,P<0.05).There was correlation between VEGF-C mRNA expression with histological grading,TNM staging,lymph node and distant metastasis (t=-1.621,-1.474,-2.378,-1.966,P<0.05).There was correlation between E-cadherin mRNA expression with tumor size,histological grading,TNM stage,there was a significant difference (t=1.875,1.673,1.544,P<0.05).VEGF-C and E-cadherin protein expression was negatively correlated(r=-0.688,P<0.05).Conclusion Up-regulated of VEGF-C gene and decreased expression of E-cadherin may play an important role in the carcinogenesis of gastric cancer