Objective To evaluate the clinical efficacy of cutaneous scraping and cupping therapy for the treatment of hyperplasia of mammary glands. Methods Eighty patients with hyperplasia of mammary glands were randomized into test group(42 cases) and control group(38 cases). Both groups were given conventional basic treatment including psychological counseling and oral use of Chinese patent medicine, and the test group additionally received cutaneous scraping and cupping therapy on acupoints of Xuanji(CV21), Danzho ng (CV17), Jianjing(GB21), Kufang(ST14) , Tianzhong(SI11), Dazhui(GV14), and Ashi(focus of mammary mass and pain). The scores of pain visual analog scale(VAS) and World Health Organization Quality of Life Scale Brief Version(WHOQOL-BREF) were observed, and the clinical efficacy of the two groups was evaluated. Results (1) The total effective rate of the test group was 88.10%, and that of the control group was 65.79%, the inter-group difference being statistically significant (P<0.01). (2) After treatment, VAS scores of the test group were decreased obviously(P<0.01), and the control group showed a decreasing trend(P>0.05). Test group had better effect on improving VAS scores than the control group(P<0.05).(3) After treatment , the scores in physiological dimension of WHOQOL-BREF were much increased(P<0.05), and the changes in the scores of other dimensions were not obvious(P>0.05). The control group showed no obvious changes in the scores of each dimension of WHOQOL-BREF before and after treatment(P>0.05). The inter-group comparison showed that the test group had better effect on improving the scores in physiological dimension of WHOQOL-BREF (P<0 . 05). Conclusion Cutaneous scraping and cupping therapy is effective on relieving pain and improving QOL of patients with hyperplasia of mammary glands.

ObjectiveAnalyze the mechanism of traditional Chinese medicine Salvia miltiorrhiza in treating coronary heart disease (CHD).MethodsThe animal experiment using actual research, namely the selection of rat isolated heart perfusion model, rat model of myocardial ischemia and ventricular tachycardia in rabbits model, then the effects of different animal groups in hemodynamics, coronary flow, serum superoxide dismutase (SOD) content, malondialdehyde (MDA) the results of the content.ResultsAfter treatment, the content of SOD was significantly increased and the content of MDA was significantly decreased in the treatment group, and the difference between the two groups was statistically significant (P<0.05).ConclusionTraditional chinese medicine Salvia miltiorrhiza has significant effect on the treatment of coronary heart disease, which is beneficial to the recovery of patients' condition, and is worthy of clinical application.

Objectives:To investigate the method of generating recombinant adenovirus vector containing human p16(Ad-p16) efficiently.　Methods:Recombinant adenovirus vector was generated with HEK 293 cells, and the titer of the adenovirus was detected.　Results:The titer of generated adenovirus is about 5×108 PFU/ml.　Conclusions:Recombinant adenovirus vector was generated with HEK 293 cells with high titer. This set the foundation of adenovirus mediated gene-transfer.

Objective To study the prevalence of intestinal metaplasia (IM) ,dysplasia and carcinoma at the distal esophagus and esophagogastric junctions as well as the relationship between SCJ and RE. Methods In 391 patients the main symptoms , RE and its degree by gastro endoscopy, and biopsy specimens from the mucosa just below SCJ were investigated. Results The prevalence of IM in LSBE,SSBE and GEJ is 26. 53% , 33.85% and 34.00% respectively; It increases with age after 40 years old and no difference occurs between' the male and female. ;12 cases of dysplasia were diagnosed ( low -grade 7 cases , medium and high-grade 5 cases) . Sixteen cases of cardia carcinomas and 1 case of esophageal adenocarcinoma were diagnosed; The longer the SCJ moved upward above GEJ, the higher the prevalence and severeness of RE . Conclusions 1. There is no difference about the prevalence of IM among LSBE,SSBE and GEJ; 2. It is important to pay attention to the diagnosis of dysplasia in the distal esophagus and esophagogastric junctions; 3. The prevalence of cardia carcinomas is much higher than that of esophageal adenocarcinoma.

Objective To evaluate the accuracy of high-aensitivity C-reactive protein (hs-CRP) in the prediction of perioperative cardiovascular accidents (CVAS) in patients with diabetes mellitus. Methods Ninetyone type 2 diabetic patients undergoing elective non-cardiac surgery were divided into 3 groups according to their blood concentrations of hs-CRP: low-concentration group ＜ 1.0 mg/L ( group Ⅰ , n = 9); median-concentration group 1.0-2.9 mg/L (group Ⅱ ,n= 16) and high-concentration group 3.0-10.0 mg/L (group Ⅲ ,n =66). Risk factors for perioperative CVA were analyzed using Spearman rank correlation analysis. Various risk factors including sex, age, ASA physical status, BMI, NYHA claasification, smoking history ,preoperative ECG changes, duration,treatment and complications of diabetes mellitus, hypertension and blood lipids were correlated with perioperative CVA. The risk factors of which P values were less than 0.05 would enter the binary logistic regression analysis to stratify CVA-related risk factors. Area under the ROC curve was used to analyze the accuracy of the risk factors in prediction of perioperative CVA. Results The incidences of perioperative CVAs were 0, 31% and 35 % in Ⅰ ,Ⅱ and Ⅲ groups respectively. It was determined by Spearman rank correlation analysis that preoperative ECG,NYHA classification, age, preoperative cardiovascular color doppler ultrasound, blood hs-CRP concentration and ASA classification of physical status were important risk factors ( P ＜ 0.05 or 0.01 ). Binary logistic regression .malysis indicated that preoperative ECG, NYHA classification and age were highly related to perioperative CVA (P ＜0.05 or 0.01).The area under ROC curve of preoperative ECG, NYHA classification, age and blood hsCRP concentration was 0.855, 0.755, 0.702 and 0.605 respectively. Conclusion The accuracy of blood concentration of hs-CRP in prediction of perioperative CVA in patients with diabetes mellitus is lower than that of the preoperative ECG, NYHA classification or age.

Objective To explore the protective effects of geranylgeranylacetone (GGA) on acute renal failure tats induced by isehemia reperfusion (IR) and the possible mechanism. Methods GGA (400 mg/kg) was administered to induce overexpression of heat shock protein 72 (HSP72) in the kidney of Sprague-Dawley (SD) rats. IR model was generated by temporary clamping the left renal artery for 45 minutes followed by right nephrectomy and 24 h reperfusion. A sham-operated group was used as normal control. 24 h after reperfnsion, rats were sacrificed. Blood was collected for measurement of serum creatinine (Scr) and blood urea nitrogen ( BUN ). Paraffin-embedded sections of the kidney were stained with PAS. Histological changes due to tubular damage were quantitated as tubular damage score. TUNEL assay was used to detect the apoptosis, and Western-blot was used to detect the expression of XIAP. Results After renal IR, the increased level of BUN and Scr, the tubular injury and the apoptosis of renal tubular epithelial cells were observed (P＜0.01). At the same time, the decreased level of XIAP was observed (P＜ 0.01). Compared with the control groups, the level of HSP72 expression was up-regulated in oral administration of GGA group (P＜0.05). The expression levels of BUN and serum creatinine were significantly decreased after IR injury in pre-conditioned rats with over-expression of HSP72 (P＜ 0.01 ). Kidney morphology was better preserved in GGA group. Rats with over-expression of HSP72 also revealed reduction of apoptotic cells by TUNEL stain and XIAP degradation by Western blot (P＜0.05). Conclusion GGA attenuates renal IR injury at least in part through inhibiting tubular cell apoptosis by decreasing XAIP degradation and restoring XIAP protein level.

Objective To analyze the epidemud growth factor receptor(EGFR)mutations in NSCLC patients in Fujian province.Methods Fresh specimens of lung cancer and corresponding normal lung tissue were collected from 50 cases of NSCLC patients.After DNA extraction,nested polymerase chain reaction (nested PCR)and direct deoxyribonucleic acid(DNA)sequencing were used to analyze EGFR gene mutations in NSCLC patients.Results EGFR mutations in tumors were identified from 13 of 50(26%)patients,including 10 cages of in-frame deletion in exon 19 and 3 cases of amino acid substitution in exon 21.Conclusion The mjor type of EGFR mutation in NSCLC patients in Fujian is in-frame deletion in exon 19.

Objective To investigate ultrasonographic (US) features of mucinous breast carcinoma with pathological correlation and to improve the early diagnosis for the mucinous breast carcinoma.Methods Twenty-two patients with 23 focuses mucinous carcinoma of the breast confirmed by pathology were included in this study,retrospectively evaluate sonographic features according to the American College of Radiology(ACR) Breast Imaging Reporting and Data System(BI-RADS) Lexicon,all cases were classified by BI-RADS before operation.Histological type of mucinous breast carcinoma included mixed and pure two forms,the latter was classified into cellular variants and hypocellular variants.The correlation between the ultrasonographic findings of mucinous carcinoma and histologic features in different types were analysed.Results On US examination,all 22 cases presented as solid mass.In pure form,they showed well-defined margins with no pseudocapsule and isoechogenic or hypoechoic internal echo pattern relative to that of subcutaneous fat in 86.7％ (13/15) focuses which histological type were pure type.93.3％ (14/15) of pure type focuses had posterior enhancement.All pure type focuses demonstrated that the orientation of the mass is parallel to the skin line.75.0％ (6/8) of mixed type focuses and 13.3％ (2/15) of pure type focuses,however,demonstrated ill-defined and or spiculated margins with more hypoechogenic structure (P ＜0.01).Mixed type focuses have more aggressive malignant features.65.2％ (15/23) of cases being classified as BI-RADS 4 or 5 were suspected as malignancy,which included 6 mixed type focuses and 9 pure type respectively accounting for 75.0％ (6/8) and 60.0％ (6/15) of each type focuses.Conclusions Mucinous breast carcinoma especial pure type did not have the typical malignant breast masses sonographic features,which showed some benign masses sonographic features except no pseudocapsule benign feature.Mixed type mucinous carcinomas tumors have more aggressive margin imaging characteristics.It is suggested that mucinous breast carcinoma should be classified category 4 by BI-RADS to avoid diagnosis delay.

Objective: To provide clinicians with diagnostic strategy and techniques for adrenal tumor by evaluating various imaging studies.Methods: The accuracy of localizing and qualitative diagnosis was analyzed by comparing various imaging examination with pathological findings after surgery combined with centesis by BUS.The clinical data of 112 patients with adrenal tumors were analyzed.Results: The pre operation lesion localizing accuracy with BUS,CT and MRI was 85.71%, 95.54%, and 98.14%,respectively.The qualitative accuracy with the same modalities as above were 33.33%,65.69%, 79.63%, respectively.For combining different imaging examination group, the accuracy of localizing and qualitative diagnosis was 100% and 86.92%,respectively.Tolally 102 cases were treated by operation and were surgically cured, of which 9 cases were treated with intervention therapy,30 cases had large tumors(exceeding 10.0 cm? 5.0 cm? 4.0 cm). Conclusion:The diversified diagnostic imaging should be applied orderly and rationality.BUS can be used as a screening method.CT and MRI are accurate in localization and differentiation of benign and malignant.Combination of various examinations has the advantages of different modalities and greatly increase the diagnostic accuracy.We should select right incision. Sufficient preparation during perioperation can reduce the complication.

Objective: To investigate the effect of intravenous immunoglobulin in highly sensitized patients awaiting organ transplantation. Methods: IVIG was used to reduce donor specific anti HLA alloantibodies in vitro and in vivo . Fifteen patients received IVIG′s suppressive experiment in vitro by random panel lymphocytotoxicity test. The serum of patients were divided into 2 groups: one was diluted with equal volume of IVIG and the other was diluted with PBS solution, and then reacted with lymphocytes from healthy donors randomly. Of them 5 patients received the treatment by IVIG. Four patients were administrated with 0.5 g/kg. Period of treatment was 4 weeks. One patient received 8 weeks infusion in same dose, 2 patients resulted in PRA drop to 10% had received kidney and pancreas kidney transplantation. Results: The percentage of RPLT in experimental group was lower than that in control group. After large dose of infusion of IVIG, the patients showed a reduction in absolute PRA of 2% 51% (mean decrease: 23%). Two patients had undergone subsequent transplantation and no serious rejection occurred. Conclusion: Treatment with IVIG is a valuable tool for the transplantation of immunized patients. The effect of IVIG is dose dependent and can be achieved in 3 weeks.