Objective To explore the relationship between the rs3785143 polymorphism of norepinephrine transporter gene,and investigate the influence of interaction effect between NET gene polymorphism and childhood abuse in adolescents with violent behavior.Methods Adolescent male offenders were divided into violent group (124 samples) and nonviolent group (120 samples).The rs3785143 polymorphisms of NET gene were measured by used SNaPshot SNP technology in 244 samples.Chi-square test was used to compare the distribution difference between three groups of each gene type and the allele frequency distribution.Results ①There was no statistically significant difference among violent group and nonviolent group in the genotype and allele frequencies of rs3785143 polymorphism (x2=0.753,0.022; all P＞0.05).②64.5％ violent adolescents have experience of childhood abuse,and 35.5％ had no history of abuse ;48.3％ nonviolent adolescents have experience of childhood abuse,and 51.7％ had no history of abuse(x2=6.50,P＜0.05).③There was not significant interaction between NET gene polymorphism and childhood abuse((OR=0.386,P＞0.05) 95％ CI (0.457-4.739)).Conclusions The NET gene polymorphism is not associated with violent behavior in male adolescents,and there was not interaction between NET gene polymorphism and childhood abuse.

Objective To study the relationship between self-esteem and childhood abuse, life events among male violent adolescent. Methods According to the modified overt aggression scale, juvenile delinquents were divided into violent group (n = 128 )and nonviolent group (n = 118 ). All the participants were investigated by Self-Esteem Scale (SES), Childhood Trauma Questionnaire-28 Item Short Form (CTQ-SF) and Adolescent SelfRating Life Events (ASLEC) and conducted correlation and regression analysis. Results ①Violent group had lower self-esteem scores ( 22.73 ± 3.30 ) than the nonviolent group ( 23.81 ± 3.30, P ＜ 0.05 ). Violent group had higher scores in physical abuse, sexual abuse, total life events, interpersonal relationship, punishment, and other (8.30 ±4.07, 7.23 ±2.26, 54.48 ±18.60, 10.09 ±3.84, 14.43 ±5.87, 4.93 ±3.15, 9.93 ±3.64), compared to the nonviolent group (7.27 ± 3.27, 6.60 ± 2.09, 45.40 ± 18.45, 8.42 ± 4.13, 11.07 ± 5.75, 3.66 ±2.81, 7.84 ±3.66, P＜0. 05). ②The self-esteem was significantly negative correlated with the childhood abuse and life events( r= -0. 143 ～ -0. 358, P＜ 0.01 ) among violent male adolescents. ③The physical abuse and punishment had significant prediction function of 15.6% on self-esteem. Conclusions Violent adolescents have low self-esteem, which is closely related to childhood abuse and adolescent life events.

Objective To explore the relationship between the rs1611115 and rs739398 polymorphism of dopamine-β-hydroxy-lase genes and violent behavior in adolescents .Methods Adolescent male offenders were divided into violent group (107 samples) and nonviolent group (107 samples) .Besides ,107 male adolescents in vocational school were served as normal controls .The rs1611115 and rs739398 polymorphisms of DβH gene were measured by used SNaPshot SNP technology in 321 samples .Chi-square test was used to compare the distribution difference between three groups of each gene type and the allele frequency distribution . Results There was statistically significant difference among violent group ,nonviolent group and normal control group in the geno-type and allele frequencies of DβH gene rs1611115 polymorphism(P<0 .05) ,but there was no statistically significant difference be-tween violent group and nonviolent group(P>0 .05) .There was no statistically significant difference among violent group ,nonvio-lent group and normal control group in the genotype and allele frequencies of rs739398 polymorphism(P>0 .05) .Conclusion No association was found between the rs739398 polymorphism of DβH gene and adolescents with violent behavior .But the rs1611115 polymorphism of DβH gene was related to adolescent criminals with violent and nonviolent behavior .

Dental Implantation, Endosseous ; methods ; Dental Implants ; Dental Prosthesis, Implant-Supported ; methods ; Humans ; Surgery, Computer-Assisted

Objective To assess the prevalence and severity of burnout in medical staffs,and investigate associated demographic,perceived social support and job burnout.Methods Totally 618 medical staffs form Sichuan province people's hospital were selected by the systematic sampling method,and were tested by general state questionnaire,job burnout questionnaire and perceived social support scale.Results ①The prevalence of job burnout was 75.6％,including mild job burnout (36.1％),moderate (32.2％),severe (7.3％).②The scores of emotional exhaustion in the female medical staffs(20.50±8.35) was higher than the male medical staffs (19.07±7.76,P＜0.05).Divorced medical staffs had higher scores(18.28±7.13) than that of single and married medical staffs (15.05±5.00) in low personal accomplishment (P＜ 0.01).The medical personnel of different ages were statistically significant difference in total job burnout scores,emotional exhaustion and depersonalisation (F=4.475,3.061,3.942,all P＜0.05).The medical personnel of different post,professional title and working years were statistically significant difference in total job burnout scores and emotional exhaustion (F=6.920,13.968; 3.506,3.391;3.190,3.300,all P＜ 0.05).The medical personnel of different educational attainment were statistically significant difference in total job burnout scores,emotional exhaustion and low personal accomplishment(F=6.218,2.837,2.850,all P＜0.05).)Correlation analysis found that emotional exhaustion,depersonalisation,low personal accomplishment and total job burnout respectively with family support,friend support,and other support and total social support was significantly negative correlation (r=-0.126～-0.403,P＜0.01).④Regression analysis showed that education,family and friends support were protective factors for total job burnout(OR=-0.248,95％ CI =0.625-0.975 ; OR=-0.084,95％ CI =0.862-0.980 ; OR=-0.091,95％ CI =0.860-0.968 ; all P＜0.05).Conclusion The prevalence of job burnout is high in medical staffs.The medical staffs of different sex,age,title,jobs,education,working years,marital status are showed the differences job burnout scores,which is closely related to the perceived social support.

Objective:To explore the association between dopamine-β-hydroxylase (DβH), norepinephrine transporter (NET) gene polymorphisms and panic disorder(PD).Methods:The structured clinical interview for the diagnostic and statistical manual of mental disorders fourth edition (DSM-Ⅳ) axis Ⅰ disorders was administered by trained clinical psychiatrist, 139 patients with PD(PD group) and 196 healthy controls(control group) were enrolled in the study.Single nucleotide polymorphism(SNP) genotyping was performed using an improved multiplex ligation detection reaction technique.SPSS 16.0 and PLINK softwares were used to compare the allele frequency and genotype distribution.Results:(1)Compared with control group, PD group carried more G allele(76.3% vs 68.4%) and fewer A allele(23.7% vs 31.6%) in NET rs5569, and the difference was significant(χ 2=4.986, OR=0.67, 95% CI: 0.47-0.95, P<0.05). However, the correlation was no longer significant after adjusting for Bonferroni’s multiple testing( P>0.05). (2)The additive model of NET rs5569 showed a association with PD ( OR=0.68, 95% CI: 0.48-0.96, P<0.05). And the recessive model of DβH rs1611114 showed a association with PD( OR=0.42, 95% CI: 0.18-0.96, P<0.05). However, these correlations were no longer significant after adjusting for Bonferroni's multiple testing( P>0.05). (3)No matter allele or genotype, there were no significant differences in DβH (rs129882, rs1611114, rs1611115) and NET (rs2242446, rs28386840) gene polymorphisms between panic disorder group and control group(all P>0.05). Conclusion:The present study indicates that there is no significant association of DβH and NET gene polymorphisms with PD.

ObjectiveTo explore the status of social anxiety and study the relationship between social anxiety and self-consciousness,self-esteem among primary students.MethodsUsing stratified randomization sampling method,568 primary students in grade 4 ～ 6 were investigated with social anxiety scale for children( SASC ),self-consciousness scale for children(PHCSS) and self-esteem scale(SES).Results①The score of SASC in the group of primary students was obviously higher than that of Chinese norm (P＜0.01).②The SASC and negative evaluation scores in the female were higher than the male (P ＜ 0.05 ),the negative evaluation scores in the sixth grade (3.22 ± 2.82) was higher than the fifth grade (2.53 ± 2.76,P ＜ 0.01 ) and fourth grade ( 2.54 ± 2.68,P＜ 0.05 ).The primary students in migrant workers elementary school,the single parent family and the relatively low income family had higher social anxiety scores than others(P＜0.05 ).③The SASC and two major factors were significantly negative correlated with the self-consciousness and each subscale( r =-0.202 ～ -0.511,P ＜ 0.01 ),and positive correlated with the self-esteem( r =0.282,0.270,0.305,P ＜ 0.01 ).④)The anxiety and gregariousness had significant prediction function of 25.5％ on negative evaluation,and 28.2％ on SASC,anxiety,gregariousness,intelligence and school situation had significant prediction function of 20.4％ on social avoidance and distress.ConclusionsPrimary students in grade 4 ～ 6 existed social anxiety.There are significant differences for their level of anxiety in different gender,grade,elementary school,family structure,monthly income.And social anxiety is closely related to self-consciousness and self-esteem.

Objective To explore the mediating effects of security on the severity of panic disorder and life events.Methods Security Questionnaire(SQ),life event scale(LES)were used to investigate 97 cases of patients with panic disorder and 108 cases of normal control group. The severity of panic disorder was assessed by panic disorder severity scale(PDSS).And the correlation and hierarchical regression analy-sis were used.Results ①The panic disorder patients' positive life stimulation(7.95±6.00)were less than that the normal control group's(18.06± 13.60),negative life stimulation and total life events stimulation ((36.64±29.98),(44.59±31.24))were higher than that of the normal control group(respectively(10.19± 7.89),(28.25±14.51)),the differences were statistically significant(all P<0.05).Panic disorder patients' interpersonal security certainty in control and total score(respectively(18.89 ± 8.66),(17.88 ± 7.58), (36.76±13.47))were lower than that the normal control group(respectively(26.64±9.33),(24.34±8.33), (50.98±15.31)),the differences were statistically significant(all P<0.01).②Severity of panic disorder and positive life events were negatively related to positive events,the total score of security,interpersonal securi-ty,certainty in control(r=-0.262--0.392);severity of panic disorder were positively related to the negative life stimulation and total life event(r=0.346,0.280)(all P<0.01).③Security played a partial mediating role between the negative life events and the severity of panic disorder(beta value decreased from 0.346 to 0.253).Conclusion The patients with panic disorder have more negative life events,lower security.And negative life events and lower security are related to the severity of panic disorder.And security plays a partial mediating effect between the negative life events and the severity of panic disorder.

Objective To explore the association between monoamine oxidase A (MAOA) variable number tandem repeat (VNTR) polymorphism and panic disorder,and then to compare panic disorder(PD) severity patient with different MAOA VNTR genotypes.Methods The structured clinical interview for the diagnostic and statistical manual of mental disorders fourth edition (DSM-Ⅳ) Axis I Disorders (SCID-1) was administered by a trained clinical psychiatrist,135 patients with PD and 195 healthy controls were recruited.MAOA-VNTR polymorphism were measured by fluorescent tags amplification product length polymorphism technology,Chi-square test was used to compare the distribution difference between each genotype and the allele frequency distribution.Results ①Whether male or female,there was no statistically significant difference between case group and healthy control group in the genotype and allele frequencies of MAOA-VN-TR polymorphism (x2=1.574,1.894,3.588;all P＜0.05).② There was no statistically significant difference between genotypes and panic disorder severity in the male with panic disorder ((14.46± 3.53),(14.15 ± 4.02);t=-0.247,P＞0.05).③)However,there was significant difference between genotypes and panic disorder severity in the female with panic disorder((13.15±3.47),(16.57±4.34),(15.27±4.91);F=4.222,P＜ 0.05).MAOA VNTR-L/L carriers experienced more serious panic (16.57 ± 4.34) than the patient with MAOA VNTR-H/H (13.15±3.47) (P＜0.01) by LSD multiple test.Conclusion No association between MAOA-VNTR polymorphism and panic disorder is found in Chinese Han population,but low activity homozygous genotype may be related to the severity of panic disorder in female patient with panic disorder.

Anxiety disorders are one of the most common mental health problems, with a high burden of disease, high morbidity and poor adherence to medication. Cognitive-behavioral therapy (CBT) is an evidence-based type of psychotherapy that works well for anxiety disorders, but it differs greatly across individuals. Studies have shown that the efficacy of CBT for anxiety disorders is related to a variety of genes, and these genes play different roles in the efficacy of CBT. There are few relatively studies in China. By exploring the possible mechanism of these genes in the effect of CBT in patients with anxiety disorders, biological markers of anxiety disorders can be further explored, which can provide reference for domestic research in this field and be applied to the diagnosis and treatment of anxiety disorders. This paper summarized the efficacy of CBT in the treatment of anxiety disorders through HPA axis related genes, 5-hydroxytryptamine system related genes, monoamine oxidase system related genes, and neurodevelopmental related genes, and found that these genes were related to the efficacy of CBT in the treatment of anxiety disorders. It mainly included methylation of FK506 binding protein 5 promoter, methylation and polymorphism of 5-HT transporter gene, gene polymorphism of tryptophan hydroxylase 2, gene polymorphism of catechol oxymethyltransferase, and gene polymorphism of monoamine oxidase A, and so on. By studying the relationship between genetics and CBT efficacy in anxiety disorders, we can explore the related loop of how pathogenic genes of anxiety disorders affect CBT efficacy, further clarify the mechanism of genetic factors in the occurrence and development of anxiety disorders, and explore the genetic predictors of CBT efficacy.