A questionnaire survey on the awareness and knowledge about stroke was conducted in 392 residents of Fangzhuang community.The results showed residents in Fangzhuang had high awareness rate about stroke,the rates of knowing early symptoms of stroke were from 57.91％ to 79.08％.83.16％ (326) of residents knew to dial 120 or 999 at emergency.The community doctors were thought as the main resource of gaining the knowledge of stroke (78.32％ ).The results indicate that Fangzhuang community health service center plays an important role in health education and guidance for emergent management of stroke in the community.

Objective:To investigate the cytokine profile expressed by CIK cells.Methods:CIK cells were induced from peripheral blood mononuclear cells in the presence of IFN-?,IL-1?,IL-2 and mAb against CD3. The phenotype and characterization of CIK cells were identified by flow cytometric analysis.Cytokine expression profiles were determined at mRNA level by semi-quantitative RT-PCR.Results:The percentage of CD3~+CD56~+ positive cells reached up to or higher than 50%.CIK cells couldn't express IFN-?、IFN-? and IFN-? under this kind of condition. But they could express IFNs principally on day 10 to 20. The level of ILs went down after steadily expressed on day 6 to 10(or 20).TNF-? and TRAIL constitutively expressed during the culture period in vitro. TNF-? maintained high expression on day 20.TGF-?_1 mainly expressed on day 30.Conclusion:CIK cells can widely express various kinds of cytokines in vitro.

Objective:To investigate the effect and elucidate the mechanism of the selective cyclooxygenase-2(COX-2)inhibitor NS-398 on apoptosis and survivin, XIAP and c-IAP1 expressions of hepatocarcinoma cell lines. Methods:The proliferation of hepatocarcinoma BEL-7402 cells treated with NS-298 at different concentrations were evaluated by MTT assay. The apoptosis was detected by flow cytometry (FCM) and TUNEL assay. Expressions of COX-2, survivin, XIAP and c-IAP1 were analyzed by immunocytochemical staining. Results: NS-398 significantly inhibited cell proliferation of BEL-7402 cells and induced apoptosis. Immunocytochemisty indicated that the expressions of COX-2, survivin, XIAP and c-IAP1 were significantly down-regulated in BEL-7402 cells by NS-398 treatment compared with untreatment group (P<0.01). Conclusion:NS-398 inhibits the proliferation and induced apoptosis of BEL-7402 cells. The mechanism may be related with down-regulation of the survivin, XIAP and c-IAP1 expression.

Objective To observe curative effect of lobaplatin plus etoposide,the new antitumor platinum drugs,on the untreated esophageal small cell of carcinoma.Methods Sixteen patients with esophageal small cell of carcinoma were treated using the lobaplatin/vp16 regimen.Results The complete response (CR) rates were 31.3%,partial response (PR)rates were 37.5%,stable disease (SD)rates were 25%,progressive disease (PD) rates were 6.25%,overall response rate (ORR)68.8%.Languor was the most common symptom in the grade -ⅠtoⅣ adverse reactions.All patients had symptoms of languor.Other related symptoms included the anaemia (41.7%), leukocytopenia (39.7%),thrombocytopenia (20.9%),anorexia,vomiting,nausea (29.3%)and increase of ALT and AST (14.5%).The toxic effect was not serious damage.Conclusion Lobaplatin is a new and safe antitumor platinum drug with a promising clinical effect,has a good tolerance in patients,and it can try to be used in the patients who have esophageal small cell of carcinoma.

Objective To develop information system for radiotherapy. Methods The radiotherapy information the system adopts B/S structure mode,ACCESS 2010 as the database Server at the front desk, running on the hospital local network,background database is called Oncentra TPS and SQL Server 2008 in Mosaiq system, using ASP programming language network, the system is in Macromedia Dreamweaver 8 platform development. Based on the Internet information services ( IIS) 6. 0 6. 0 build system server service components,IE browser is used to implement the client access server capabilities. Results The information system including system Settings module,physics teacher module,the doctor module,technician module,data statistics and analysis, data download, seven modules such as video teaching. Conclusions The Radiotherapy information system is real?time performance, data security, stable operation, is the key construction for efficient utilization of resources in radiotherapy.

Objective To construct the angiotensinogen (AGT)-targeting RNA interfering plasmids, and to explore the effect of AGT gene on the differentiation of preadipocytes 3T3-L1 in the mice.Methods The small hairpin RNA (AGT-shRNA)interference plasmids,with a scrambled Non-shRNA as control,were constructed on the basis of the activity of U6 promoter-driven expression vector psiRNA-U6.1/Neo and transfected into the mouse preadipocytes 3T3-L1.Two cell lines were generated from stable transfections.RT-PCR and SDS-PAGE were performed to monitor the mRNA and protein expressions of AGT gene,respectively.The effect of adipose AGT on the differentiation of 3T3-L1 was investigated through microscopic observation and profiling of adipocyte differentiation markers. Results Both AGT-shRNA and Non-shRNA interference vectors were successfully constructed;the expression of AGT mRNA was inhibited (P < 0.05 ) and the expression of AGT protein was 43.86% of control.Both adipocyte differentiation markers,intracytoplasmic lipid levels and glycerol-3-phophate dehydrogenase (GPDH)activities were increased during the differentiation of preadipocytes 3T3-L1,but the data were lower than those in control group (P <0.05).Conclusion AGT gene silencing can significantly inhibit the differentiation of preadipocytes 3T3-L1,which demonstrates that there is important relationship between adipocyte metabolism and renin-angiotensin system (RAS)in adipose tissue.

Objective To establish HPLC fingerprint of Radix Gentianae Crassicaulis.Methods The fingerprints were obtained on an Ultimate XB-C18 column(250 mm?4.6 mm,5 ?m)with the gradient elution solvent system composed of acetonitrile and 0.05% phosphoric acid.The flow rate was 1.0 mL/min,the column temperature was maintained at 25 ℃,and the detection wavelength was set at 240 nm.Results Ten common peaks were selected as the fingerprint peaks and the fingerprints also were evaluated by the Similarity Evaluation System for Chromatographic Fingerprint with the correlation coefficient of 0.98—1.0.Conclusion This method with good precision and reproducibility is reliable for the quality control of Radix Gentianae Crassicaulis.

Pharmacokinetics of Tetramethylpyrazine Hydrochloride (TMPH) in rat was studied by using Ultraviolet Spectrophotometry. After a bolus iv injection of TMPH 30 mg/kg to rat, the pharmacokinetic characteristics are found to fit a two-compartment open model. The Pharmacokinetic parameters are: t 1/2? = 0 .1441h, t 1/2? = l .6953h, K21=2.1850h-1, K10=0.8605h-1, K12= 2 .0723h-1, AUC = 83 .3660mg?L -1h, CL = 0.3599L?kg-1h-1, Yc =0 .4182L?kg-1 , Vss =0 .7975L.kg-1

In order to improve the result of clinical practice for undergraduate students of sto-matology, the standardized training program was carried out before clinical practice by the teaching and researching Office of Periodontology of Affiliated Stomatological Hospital of Chongqing Medical University, in this program, based on our own realities, the structure and content of teaching was designed to guide the transformation of students to doctors, collective teaching and personalized teach-ing was combined to reform the conventional model of teaching and various student-oriented teaching methods were explored, including mimic forum of disciplines, intractable case discussion, clinical practice summary, course completion test and so on, thus promoting the result of clinical practice. Be-sides, the construction of teaching staff was strengthened, and the evaluation and management system was established to ensure the teaching quality, thus summarizing a set of efficient and effective clinical teaching methods presented in this paper.

Objective: To investigate the effects of the cooperative actions of isoflavone, vitamin C and E on the expression of acute C reactive protein (CRP) and interleukin 6 (IL-6) in the patients with typeⅡdiabetic mellitus (DM). Method: Ninety six patients with DM were divided randomly into 4 groups, DM control group(B), and groups receiving low(C), medium (D) , and high (E) dose of the compound antioxidant of isoflavone, vitamin C and E respectively. Another 24 healthy subjects served as normal control group (A). Observe the changes of the levels of CRP and IL-6 during oral glucose tolerance test (OGTT). Results: The peak of serum levels of CRP and IL-6 in the patients of DM control group and lower dosage group appeared 1 h after OGTT and were significantly higher than the 0 h value (P