Objective:To investigate the potential value and feasibility of MG132 as a new therapy method for the ovarian cancer and a cisplatin chemotherapy-synergistic agent.Methods:The mode of the nude mice transplanted tumor tissues of ovarian epithelial carcinoma were established,20 nude mice were randomly divided into four groups and were all intrapedtoneal injected,once a day,a total of seven days:①Control group(0.2 ml saline),②MG132 group(2 mg/kg),③Cisplatin group(1 mg/kg),④Combination group[MG132(2 mg/kg) + cisplatin (1 mg/kg)].The weight inhibitory rates of tumors in each group were compared after four weeks.The expressions of Caspase3,Beclin1 in each group were detected by IHC,FIA,western blot and RT-PCR.Results:①The inhibitory rate of tumors in cisplatin group,MG132 group,and combination group was 53.85％,15.38％,88.46％,respectively,the additive effect of cisplatin and MG132 combination therapy was 60.95％.②IHC,FIA,western blot detected that compared to control group,the positive expressions of Beclin1,Caspase3 were increased in cisplatin group,MG132 group,and combination group,among which combination group increased more.③RT-PCR detected that the mRNA relative quantity of Beclin1 in cisplatin group,MG132 group,combination group respectively were higher than that of control group(P＜0.05);and it was higher in combination group than that of cisplatin group and MG132 group(P＜0.05).Conclusions:The growth of nude mice transplanted tumor tissues of ovarian epithelial carcinoma can be inhibited by MG132,and has a synergistic effect for treating ovarian cancer by combination with cisplatin,it is expected to be an effective anti-tumor drug for platinum resistant refractory ovarian cancer.

Objective To study in vitro ICAM 1 and P selectin expressions of endothelial cells treated with sera from intrahepatic cholestasis of pregnancy (ICP). Methods HUVEC were cultured and stimulated with sera, and the expression of ICAM 1 and P selectin by flow cytometry were measured. Results (1) The expressions of ICAM 1 of P selectin on the HUVEC surface after stimulation with sera from women with ICP were higher than those of without stimulation in normal pregnancy ( P

Objective To study the effect of thalidomide(Thd) used alone and in combination with cytoxan (CTX) on the growth and angiogenesis of human ovarian cancer transplanted subcutaneously in nude mice. Methods Human ovarian cancer model transplanted subcutaneously in nude mice was established, and divided into 3 groups: control group, Thd group, and Thd+CTX group.Tumor volume and weight,vascular endothelial growth factor(VEGF) mRNA, VEGF protein, microvascular density(MVD) were detected. The level of VEGF mRNA in tumor tissue was determined by relative quantative reverse transcription polymerase chain reaction. VEGF protein level in serum was determined by enzyme-linked immunosorbent assay (ELISA). MVD was calculated by immunohistochemistry. Results (1)Tumor volumes in Thd group and Thd+CTX group were smaller than those in control group(P

Objective To investigate the expression of p53 in human ovarian neoplasms by tissue array technique Methods The expression of p53 protein in various ovarian tissues was studied by tissue array and immunohistochemistry Results The expression rate of p53 was 33% in ovarian cancers There were no expressions in normal ovarian tissues, benign ovarian neoplasms and borderline ovarian neoplasms( P 0 05) while p53 expression was associated with tissue types( P

Objective To study the angiogenesis in serous ovarian neoplasms. Methods Immunohistochemical method was used to examine microvessei density (MVD) and the expressions of vascular endothelial growth factor (VEGF) in 76 cases of serous ovarian neoplasm. The relationship between MVD and VEGF were investigated. Results MVD in the tissues of cystadenoma, bordline cystadenoma and cystadenocarcinoma were 7.8±2.4, 17.5±3.3 and 27.6±7.5; VEGF expressions were (16.1±3.9)%, (36.7±14.2)%, (52.3±22.8)%; the differences were significant respectively. Of cystadenocarcinoma grade 1, grade 2 and grade 3, MVD were 18.3+3.4, 24.2±3.1, and 32.3±6.4, VEGF expressions were (30.9±16.2)%, (48.0±18.4) % and (62.1±21.3) %, the differences were significant respectively. Of FIGO stage Ⅲ～Ⅳ and stage Ⅰ～Ⅱ, MVD were 28.9±4.7 and 22.0±4.7, VEGF expressions were (55.1±22.9) % and (40.18±18.0) %, the differences were significant respectively. There was significant positive correlation between VEGF expression and MVD. Conclusion Angiogenesis is very important in the development of serous ovarian neoplasms.

OBJECTIVEMinimal deviation adenocarcinoma (MDA) is frequently underdiagnosed in pathology tests due to its benign histopathological features. Therefore, its clinical and pathological characters need to be further analyzed and to establish useful immunohistochemical markers to improve the accuracy of pathological diagnosis.

METHODSClinical pathology data were of 13 MDA cases collected and analyzed in the First and Second Hospitals attached to the University, the pathological characters were compared with matched benign hyperplastic and atypical hyperplastic controls of the glandular epithelial cells to find histochemical and immunohistochemical indices valuable for the pathological diagnosis. 8 of these 13 cases were followed and their conditions were discussed.

RESULTSThe main clinical symptoms were watery leucorrhagia, enlargement of the cervix with erosion and hardening. Thickened ligaments were also detected in some cases. The pathological findings included hyperplasia and mild abnormality of the glands, invasion effects into the stroma could be observed in some glands and abortive glands with desmoplastic changes, or edema and inflammatory infiltration around the glands were also observed. The invasion presented in the deep part of the cervix as well, some were found near larger blood vessels or in the small vessels. Compared with benign glands, the immunohistochemical indices of most MDA glands were demonstrated as cancer embryo antigen (CEA) + + +, Ki-67 +--+ + +, P53 +--+ + +, AB/PAS +/+ +, and focal defect on the basement membrane.

CONCLUSIONSOnce any of the diagnostic characters summarized as above was determined, the probability of MDA should be seriously considered, at least atypical hyperplasia in cervical glands must be diagnosed, and follow-up on the patients and histological biopsy of deep tissues (> 5 mm) of the cervix will be necessary to determine the inclusion/exclusion of MDA. Obviously, a correct pathological diagnosis is extremely important for the patient to receive proper treatment in time and thereby improve her quality of life.

Adenocarcinoma ; pathology ; Adult ; Aged ; Cervix Uteri ; Diagnosis, Differential ; Female ; Humans ; Immunohistochemistry ; Middle Aged ; Uterine Cervical Neoplasms ; pathology

This study was designed to construct THY1 eukaryotic expression plasmid and assess its effects on epithelial ovarian cancer cell line SKOV3. The gene fragment coding for THY1 was inserted into pcDNA3.1(+) for constructing the recombinant plasmid pcDNA3.1(+)-THY1. The eukaryotic expression plasmid was analyzed by PCR, restriction endonucleases digestion and DNA sequencing. The recombinant plasmid pcDNA3.1(+)-THY1 was transfected into SKOV3 cells by liposome protocol. The experimental cells were classified into three groups: SKOV3-THY1, SKOV3-Null and SKOV3. The pcDNA3.1(+)-THY1 has been transfected into SKOV3 cells by RT-PCR and immunohistochemistry. The cell inhibitory rate of SKOV3-THY1 (56.6% at the fifth day) was higher than that of SKOV3-Null (12.5%), there was significant difference between them (P<0.05). The ratios of G1 phase of SKOV3 cells after transfection were increased and the ratios of S phase were decreased significantly. There was significant difference between SKOV3-THY1 and SKOV3-Null or SKOV3 (P<0.05), but there was no significant difference between SKOV3-Null and SKOV3 (P>0.05). We have constructed the recombinant plasmid pcDNA3.1(+)-THY1 sucessfully. THY1 transfection can inhibit the growth of SKOV3 cells in vitro.
Cell Line, Tumor ; Cell Proliferation ; drug effects ; Cloning, Molecular ; Female ; Genetic Vectors ; genetics ; Humans ; Ovarian Neoplasms ; pathology ; Plasmids ; genetics ; Recombinant Proteins ; biosynthesis ; genetics ; Thy-1 Antigens ; biosynthesis ; genetics ; Transfection

The shRNA expression vectors were constructed and transfected via lipofectamine into HeLa cells. Real time-ploymerase chain reaction (RT-PCR) and Western blot were used for detecting the expression of mRNA and protein of Beclin1 in transfected cells. Flow cytometry was employed to observe the effect of transfection on the apoptosis and cell cycle of HeLa, and proliferation was analyzed by MTT assay. The expression of caspase-9 in transfection cells was also detected by RT-PCR and Western blot. The constructed vectors significantly inhibited the expressin of mRNA and the protein of Beclin1 in HeLa cells. The growth of transfected cells was promoted, and less apoptosis cells were identified in these cells. After transfection of the constructed vectors into HeLa cells, the expression of caspase-9 was effectively inhibited. All of these indicate that autophagy and apoptosis are two types of programmed cell death, that autophagy gene Beclin 1 plays an important role in these two types, and that defect of autophagy and apoptosis may be important in tumor genesis.
Apoptosis ; genetics ; Apoptosis Regulatory Proteins ; genetics ; metabolism ; Autophagy ; genetics ; Beclin-1 ; Caspase 9 ; metabolism ; Down-Regulation ; Genetic Vectors ; HeLa Cells ; Humans ; Membrane Proteins ; genetics ; metabolism ; RNA Interference ; RNA, Messenger ; genetics ; RNA, Small Interfering ; genetics ; Transfection

Objective To determine the clinicopathologic characteristics and prognostic factors that may be used to predict the poor outcome of patients with borderline ovarian tumors. Methods All cases with borderline ovarian tumors treated in the West China Second University Hospital from January 2001 to June 2007 were analyzed retrospectively for elinicopathologic features, treatment parameters and outcome of treatment. Univariate and multivariate analyses were used to assess independent prognostic factors using the logistic regression model. Results The median age of 234 patients was 40. 1 years with a range of 14 to 80 years. There were 101 (43.2%), 94 (40.2% ) , 19 (8.1% ), 12 (5.1%) , 8 (3.4%) cases of serous, mutinous, mixed, endometrioid and clear cell tumors, respectively. Out of 234 cases, 182 (77.8%) underwent laparotomy and 45 ( 19.2% ) underwent laparoscopy. Seven women underwent laparoconversion. Fertility sparing surgery was performed on 119 cases (50.9% ) and radical surgery was performed on 115 cases (49.1% ). Totally 161 (68.8% ) patients had stage Ⅰ , 19 ( 8.1% ) had stage Ⅱ, 54 ( 23.1% ) had stage Ⅲ, and none had stage Ⅳ disease. Sixty-four women received postoperative chemotherapy. The median follow-up was 40 months with a range of 8 to 78 months. Recurrence was found in 26 cases (11.1%) during follow-up, and no tumor-related death was reported. The logistic regression model showed that surgery procedure ( OR=2.304, P=0.024), cyst rupture ( OR=2.213, P=0.038 ), stage ( OR= 4.114, P<0.01 ), microinvasion ( OR=2.291, P=0.046) and peritoneal implants ( OR=2.101, P = 0.016) were the five independent prognostic factors affecting recurrence. Conclusions Although patients with borderline ovarian tumors have an excellent prognosis, the risk of recurrence remains in some patients. Emphasis should be put on these patients with high risk factors and preventive strategies should be taken to prevent their progression.

Anti-Inflammatory Agents ; pharmacology ; Cells, Cultured ; Cholestasis, Intrahepatic ; metabolism ; pathology ; Dexamethasone ; pharmacology ; Female ; Fluorescent Antibody Technique ; Humans ; Intercellular Adhesion Molecule-1 ; drug effects ; metabolism ; P-Selectin ; drug effects ; metabolism ; Pregnancy ; Pregnancy Complications ; Tumor Necrosis Factor-alpha ; pharmacology