Objective To observe the effect and safety of ambroxol combined with antibiotic in treatment the elderly diabetic patients complicated with pneumonia.Methods 86 elderly diabetic patients with pneumonia were selected and divided into observation group and control group according to different treatment options.All the patients were given oxygen,suction,nutrition therapy,postural drainage and basic treatment for diabetes.Cefoperazone sulbactam was given in control group,5.0g dissolved in 150 ml saline,1 time/d.Ambroxol hydrochloride injection was added in observation group,40mg/kg dissolved in 150 ml saline,1 time/d.Body temperature,sputum,leukocyte and chest X-ray were observed and recorded,and the recovery time was recorded.Results 37 cases were cured,6 cases improved in observation group,and the total effective rate was 100.00％.28 cases were cured,12 cases improved in control group,and the total effective rate was 93.02％.The effective rate of the observation group was higher than the control group(P ＜0.05).The incidence rate of adverse reactions in observation group was 6.98％,while 4.65％ in control group,and there was no statistical difference between the two groups (P ＞ 0.05).Conclusion Ambroxol hydrochloride added to antibiotic routine therapy in treatment the elderly diabetic patients complicated with pneumonia couldimprove the cure rate and shorten the healing time.It was worthy of clinical application.

Objective To analyze the clinical treatment effect of edaravone on vascular Parkinson syndrome. Methods 78 patients in our hospital who accepted treatment were selected as the study samples.They were divided into two groups which included treatment group (n =39)and control group (n =39)by random number table meth-od.The control group were given routine treatment,mainly including madopar,aspilin and cripar,however,the treat-ment group administered edaravone on the basis of conventional treatment.After total 2 periods of treatment,the score of UPDR,therapeutic efficacy and adverse reactions were recorded and compared.Results The therapeutic effective rate was 92.31% in the treatment group,which was significantly higer than that of the control group (66.66%),and the difference was statistical significance (χ2 =4.251,P <0.05).After treatment,scores and motor function in two groups of patients with activities of daily living score were decreased,but the difference was no statistical significance (P >0.05).The patients in the observation group mental,e -motional and behavioral score were lower than those of the control group (t =4.568,4.113,4.345,all P <0.05),and there was no adverse reaction in the treatment group. Conclusion The treatment of edaravone in vascular Parkinson syndrome can obviously enhance the therapeutic effect,improve the treatment efficiency,has less adverse reaction and good tolerance,it is worth for clinical reference.

Objective To study the effect of thalidomide(Thd) used alone and in combination with cytoxan (CTX) on the growth and angiogenesis of human ovarian cancer transplanted subcutaneously in nude mice. Methods Human ovarian cancer model transplanted subcutaneously in nude mice was established, and divided into 3 groups: control group, Thd group, and Thd+CTX group.Tumor volume and weight,vascular endothelial growth factor(VEGF) mRNA, VEGF protein, microvascular density(MVD) were detected. The level of VEGF mRNA in tumor tissue was determined by relative quantative reverse transcription polymerase chain reaction. VEGF protein level in serum was determined by enzyme-linked immunosorbent assay (ELISA). MVD was calculated by immunohistochemistry. Results (1)Tumor volumes in Thd group and Thd+CTX group were smaller than those in control group(P

Objective To develop infant thermal container with forced protection circuit so as to ensure its security. Methods Temperature database was set in thermal container and Cygnal F005 was used as intelligent controller. The state of the sensor was judged by comparison precisely. Double temperature controller was adopted to ensure normal working temperature. When the container went wrong, it could automatically switch to protection circuit to keep the temperature for infant. Results With precise temperature control and steady working station, the new type thermal container for infant could provide a safe and comfortable condition with clean air and fitting temperature and humidity. Conclusion The container is steady, accurate, safe and convenient.

Objective To analyze the change of surgical route and range of hysterectomy in the past 10 years.Methods From January 2000 to December 2010,the clinical data of patients with hysterectomy were analyzed retrospectively,including clinical features,surgical path and scope of operation.Results There were 1980 patients.The predominant reason for hysterectomy was leiomyomas (52.88％,1047/1980),adenomyosis (17.78％,352/1980),cervical intraepithelial neoplasia (12.17％,241/1980).The age of the patients with hysterectomy for abdominal,transvaginal and laparoscopic was (46.54 ± 7.35),(58.73 ± 10.49),(44.10 ±5.12) years.In 2000-2005,the proportion of abdominal,transvaginal and laparoscopic proportion were 81.78％ (615/752),8.64％ (65/752) and 9.57％ (72/752).In 2006-2010 were 64.50％ (792/1228),6.51％ (80/1228) and 28.99％ (356/1228).In 2006-2010,the proportion of abdominal significantly decreased and laparoscopic significantly rised compared with those in 2000-2005,there were statistically significant differences (P ＜ 0.01).In 2000-2005,unilateral and bilateral annex removal ratios were 15.43％ (116/752),12.23％ (92/752),in 2006-2010 were 8.63％ (106/1228),7.49％(92/1228).Unilateral and bilateral annex removal ratios in 2006-2010 were significantly lower than those in 2000-2005,there were statistically significant differences (P ＜ 0.01 or ＜ 0.05).Conclusions Over the past 10 years,hysterectomy route and range changed with the percentage of laparoscopic increasing,and concomitant unilateral or bilateral annex removal decreased.

In the past ten years, 52 patients with double-chambered right ventricle (DCRV) were operated on in Xijing Hospital. They constituted 2.2% (52/1881) of all patients with congenital heart diseases. Of them, there were 32 males and 20 females, whose ages ranged from 4.5 to 30 years old. In one patient there was pure DCRV, and the remaining 51 patients DCRV was associated with other congenital cardiac anomalies, in which VSD was the most common (50), followed in order by pulmonary stenosis (6), aortic valve prolapse (4), atrial septal defect (2), and subaortic stenosis (1). The repair of DCRV and other intracardiac defects was done through the right ventricolotomy in 47 cases and through the right ventricolotomy plus atriotomy in 2 cases. In another 2 cases the operation was done via the right atriotomy and 1 case pulmonary arteriotomy, and only VSDs were closed, but DCRVs were missed. The major postoperative complications included cardiac arrhythmia (11) and low output syndrome (8). Three patients died, with the operative mortality of 5.8%. In this series, according to characteristics of the anomalous muscle bundle and its resulting obstruction, we divided 52 case DCRVs into two types: fibromuscular diaphragmatic type (24 cases, 44.7%) and muscular bundle-gap type (28 cases, 55.7%). In both types, there were not only hypertrophied anmalous muscle bundles on the septal side, but also hypertro-phied ventricoinfundibulum fold on the parietal side. In a few cases, the ventricoinfundibulum fold was more hypertrophic than the anomalous muscle bundle on the septal side.

Objective To explore the effect of separate management of income and expenditure (SMIE) on standard community healthcare services. Methods Health management records of 137 community health service ( CHS) institutions in Beijing during 2006 and 2009 were retrospectively analyzed. Paired t test was used to compare annual healthcare services at urban and rural areas before or after SMIE was adopted. Results The standard community healthcare services was reduced from 1 081 965. 53 to 971 793. 98 at urban areas and from 157 492. 23 to 149 305. 25 at rural areas (P >0. 05). Urban healthcare center-based standard services was decreased from 59. 94% to 52. 14% (P < 0. 05 ); while out-hospital services were increased from 13. 50% to 18. 88% (P<0. 05). In urban areas,standard services for women healthcare, chronic disease profiling, health education and information management were significantly increased (I <0. 05). However,Temporary observation and endemic disease prevention and treatment were the main job of rural healthcare. Conclusions SMIE in CHS institutions could accommodate to the need of health management, supporting policies should be further improved.

Objective To study the angiogenesis in serous ovarian neoplasms. Methods Immunohistochemical method was used to examine microvessei density (MVD) and the expressions of vascular endothelial growth factor (VEGF) in 76 cases of serous ovarian neoplasm. The relationship between MVD and VEGF were investigated. Results MVD in the tissues of cystadenoma, bordline cystadenoma and cystadenocarcinoma were 7.8±2.4, 17.5±3.3 and 27.6±7.5; VEGF expressions were (16.1±3.9)%, (36.7±14.2)%, (52.3±22.8)%; the differences were significant respectively. Of cystadenocarcinoma grade 1, grade 2 and grade 3, MVD were 18.3+3.4, 24.2±3.1, and 32.3±6.4, VEGF expressions were (30.9±16.2)%, (48.0±18.4) % and (62.1±21.3) %, the differences were significant respectively. Of FIGO stage Ⅲ～Ⅳ and stage Ⅰ～Ⅱ, MVD were 28.9±4.7 and 22.0±4.7, VEGF expressions were (55.1±22.9) % and (40.18±18.0) %, the differences were significant respectively. There was significant positive correlation between VEGF expression and MVD. Conclusion Angiogenesis is very important in the development of serous ovarian neoplasms.

OBJECTIVE Toinvestigatethedifferenceofcytotoxiceffectsofhydroxycamptothecin(HCPT)onhuman lungcancercelsA549andhumanembryolungfibroblastcelsMRC-5.METHODS A549celsandMRC-5celswere treated with HCPT 20-200 μmol·L-1 for 24,48 and 72 h,or pulse treated with HCPT 50-400 μmol·L-1 for 24 h along with 5 d release.cellsurvival was detected by MTT assay.Morphological changes for both types of cells were observed under an inverted phase-contrast microscope.cellcycle and apoptosis in both cells treated with HCPT 50 μmol·L-1 for 48 h weredeterminedbyflowcytometry.RESULTS HCPT20-200μmol·L-1inhibitedthesurvivalofbothcelsinaconcen-tration-dependent manner and more cytotoxicity was observed in A549 cells for 48 h.The concentration-effect correlation coefficient(r)of HCPT in A549 and MRC-5 cells for 48 h was 0.898 (P=0.015)and 0.996 (P=2.56E-5)respectively. The inhibition rates were significantly different between A549 and MRC-5 cells with treatment of HCPT 20,50,80,1 00, 1 60 and 200 μmol·L-1 for 48 h (P<0.05).The IC50 of HCPT on A549 and MRC-5 cells was (24.00 ±0.69)μmol·L-1 and (1 23.63 ±3.89)μmol·L-1 respectively,indicating that A549 cells were 5-fold more sensitive to HCPT than MRC-5 cells at 48 h.After exposure to HCPT 50 μmol·L-1 for 48 h,some A549 cells were rounded up and shrank dramatical y, and some cells underwent membrane blebbing or lysing while MRC-5 cells had no obvious changes.cellcycle and apop-tosis analysis showed that A549 cells were arrested at both S and G2/M phases and apoptosis occurred but MRC-5 cells were just arrested at S phase.In the recovery growth curve,the growth of A549 cells was inhibited to a larger extent than MRC-5 cells and the growth retardation stil existed for 24 h in both cells.The survival of MRC-5 cells was faster than that ofA549cels,althoughtherewasnocompleterecoveryineithercel.CONCLUSION A549celsaremoresensitiveto HCPT than MRC-5 cells due to the fact that HCPT induces cellcycle arrest at both S and G2/M phases and apoptosis in A549 cells,but only triggers S phase arrest in the MRC-5 cells.

Objective To evaluate the application value of 256 slice spiral CT angiography (CTA)in the bridge vessels lesions af-ter grafts operation in lower extremity arteriosclerosis obliterans patients.Methods Taken DSA as the gold standard,using 256 slices spiral CTA to analysis the post-processing image of 76 article Bridge vascular which can be assessed in 65 cases patients with lower extremity arteriosclerosis obliterans,and to evaluated the diagnosis accuracy,sensitivity,specificity,positive predictive rate and negative predictive rate of postoperative bridge intravascular lesions.Results Using 256 slices spiral CTA,the bridge vascular of grafts operation patients can be clearly displayed,stenosis or unobstructed,and the anastomosis of the bridge vascular.5 graft occlu-sion,22bridge intravascular plaque were diagnosed correctly,3 vascular lesions were undiscovered.The accuracy,sensitivity,speci-ficity,positive predictive rate and negative predictive rate were 98.15%,96.15%,97.90%,92.59% and 97.96% respectively. Conclusion Using 256 slices spiral CTA can clearly show the endovascular lesions of lower extremity arteriosclerosis obliterans after grafts operation,and has high accuracy and specificity.It can be safely used as one of noninvasive follow-up means after grafts oper-ation.