BACKGROUND:Simple medication therapy can not promote the healing of articular cartilage defects effectively;the insufficient sources of autogenous cartilage also limited cartilage transDlantation.OBJECTIVE:The types of articular cartilage injury and local micro-environment changes were analyzed to summarize the progress of tissue engineered seed cell transplantation and cell transplantation therapy for repairing articular cartilage injury.METHODS:The databases of PubMed database(http://www.ncbi.nlm.nih.gov/sites/entrez/)and CNKI(http://www.cnki.net/)were retrieved with the key words of"Tissue engineering,cell transplantation,articular cartilage defects",and the literature was limited to English and Chinese languages.Studies concerning articular cartilage injury or was closely related to tissue engineered seed cells transplantatiOn were included.Repetitive studies were excluded Survival and migration of seed cells,as well as the recovery of joint function and adverse reactions were served as evaluation index.RESULTS AND CONCLUSION:A total of 201 literatures were seized by computers,according to the inclusion criteria,papers concerning tissue engineering cells for repairing articular cartilage injury were analyzed.Clinically,articular cartilage damage was commonly resulted by traumatic arthritis,which is difficult to repair due to the poor self-repair capacity of articular cartilage.The emergence of tissue engineered cell transplantation brought new hope for articular cartilage injury healing Cell transplantation has obtained good effects in articular cartilage repair,but the technology is still room for improvement.How to technically improve the tissue-engineered 3 elements,namely,cells,scaffold materials,and biological activity factors were the research focuses.Autologous cartilage regeneration was the theoretical support for articular cartilage defects repairing,which needs to optimize its regeneration and to maintain a more stable chondrocyte phenotype in further research The tissue-engineered cells had received good effects in repairing articular cartilage injury,and it is expected to be a new clinical treatment for articular cartilage injury with the in-depth study of tissue engineering.

Brain-computer interface(BCI) was a communication system that did not depend on the brain's normal output pathways of the peripheral nerves and muscles.BCI provided a new communication channel for the patients with severe neuromuscular disorders,and also had potential values in virtual reality,entertainment and aviation.Recently,BCI developed rapidly,and its researches mostly concentrated on feature extraction and translation algorithm.Cortical evoked potentials such as steady-state visual evoked potential(SSVEP) and P300 were widely applied in BCI because of simple feature extraction and requiring little training.The progress in SSVEP-based and P300-based BCI was reviewed,and its experimental paradigms and limitations were analyzed in this paper.

Objective Though X-ray measurement of metacarpal bones of right hand in children aged 7-12 in Kaschin-Beck disease (KBD) endemic areas in Xinghai County,Qinghai Province,to provide scientific basis for KBD diagnosis and research on growth and development of children in KBD endemic areas.Methods In 2012,277 children aged 7-12 were carried out clinical examination and right hand X-ray photos in KBD endemic areas,Xinghai County,Qinghai Province.Metacarpal bones were measured by electronic digital vernier caliper,and the axis length and width of the metacarpal bones and its metacarpal bone index were calculated.All data were analyzed by SPSS 19.0 software.Results The axis lengths of the metacarpal bones were in an order of 2.metacarpal ＞ 3.metacarpal ＞ 4.metacarpal ＞ 5.metacarpal ＞ 1.metacarpal in 6 different age groups and different genders; the width of 1.metacarpal bone was the widest; the width of 4.metacarpal bone was the smallest,and the rest of metacarpal bones were similar.The metacarpal bone indexes of female [(7.96 ± 0.76),(8.24 ± 0.92),(8.24 ± 0.71),(8.26 ± 0.92),(8.60 ± 1.02),(8.67 ± 0.84)mm] were higher than those of male's[(7.30 ± 0.78),(7.53 ± 0.77),(7.98 ± 0.90),(7.79 ± 0.56),(7.96 ± 0.64),(8.17 ± 0.92)mm,F =4.302,P ＜ 0.05].Conclusion The axis length,width and index of metacarpal bones of right hand in children between KBD endemic areas and no-KBD endemic areas are similar.

ObjectiveTo explore the relation of the expression of intron 9 in CD44 gene,nm23 gene and the serum levels of TGFα and TNFα to the development, metastasis and prognosis of epithelial ovarian cancer. Methods The expressions of intron 9 in CD44 gene, nm23 gene, TGFα and TNFα in epithelial ovarian cancer were measured by semi -quantitative RT - PCR, immunohistochemical technique and radioimmunoassay ( RIA ). Results There was positive expression of intron 9 in CD44 gene,nm23 gene,TGFα and TNFα in 36 cases of epithelial ovarian cancer. The higher levels of nm23 gene and TGFa were obvious in early stage of ovarian cancer. ConclusionTo study the ovarian cancers biologic behavior with these multipe factors can provide theoretical basis for early diagnosis, gene therapy and predicting prognosis.

Objective: To establish the quality control method for ephedrine in Herba Ephedrae and its preparation Zhisoulixiao Tablets (Herba Ephedrae, Pericarpium Papaveris, Semen Armeniacae Amrum, Radix Glycyrrhizae, Gypsum Fibrosum, Semen Lepidii, Semen Raphani, etc.) and Zhisoulixiao Capsules. Methods:Ephedrine hydrochloride contents in Herba Ephedrae and its preparation Zhisoulixiao Tablets and Zhisoulixiao Capsules were determined by TLC scanning with a mixture of chloroform ethyl acetate methanol water(13∶40∶22∶10)as a developer and single wavelength reflection sawtooth scanning. Results: The methodological study showed that a good linear correlation in the range from 0.29112?g to 1.4556?g of the ephedrine hydrochloride applied. The average recovery of ephedrine hydrochloride in Herba Ephedrae, Zhisoulixiao Tablets and Zhisoulixiao Capsules was 78.74%( n=5 ),99.46%( n=5) and 99.14%( n=5) ,respectively. Conclusion: The method established is simple,accurate with good reproducible and high precision.The method can be used for the quality control of Herba Ephedrae and its preparation

AIM: To establish a simple and rapid method for the quality control of Chinese traditional medicine. METHODS: Pericarpium Citri Reticulatae,Fructus Psoraleae,Rhizoma Atractylodis macrocephalae and Radix Codonopsis in Wenweishu Tablets were identified by TLC at two thin-layer chromatographic plates.The contents for hesperidin,psoralen and isopsoralen in this preparation were determined simultaneously by HPLC. RESULTS: The methodological study showd that a good linear correlation existed in the range of 0.039 6-0.495 ?g(r=(0.999 99)) of hesperidin,0.018 5-0.231 ?g(r=0.999 98) of psoralen and 0.019 7-0.246 2 ?g(r=0.999 91) of isopsoralen applied respectively.The average recovery of hesperidin was 98.42%(n=9),RSD was 2.69%;The average recovery of psoralen was 98.29%(n=9),RSD was 1.08%.The average recovery of isopsoralen was(98.93%)(n=9),RSD was 2.41%. CONCLUSION: The method is simple、rapid and practicle.

Objective To estimate the prevalence of erectile dysfunction (ED) among the patients undergoing manintenance hemodialysis (MHD), and evaluate the efficacy and the adverse effects of sildenafil for ED. Methods The international index of erectile function questionnaire (IIEF) was used to estimate 22 male MHD cases. ED patients were administered sildenafil with the initial dose of 25 mg/d, then maximum of 100 mg/d for 12 weeks. Results 72. 73% (16/22) of MHD patients complained of ED, and the total effective rate of sildenafil was 87.5% (14/16) with common adverse effects-headache and flushing. Conclusion Sildenafil in MHD patients has similar efficacy and safety as the others.

Objective To observe the clinical effects of different doses of Xuebijing injection in the treatment of sepsis. Methods Eighty patients with sepsis from First Center Clinical College of Tianjin Medical University were selected and randomly divided into four groups:the control group,Xuebijing small,middle and large dose groups(each, 20 patients). All patients in the four groups were given Western medicine conventional treatment,whereas in the Xuebijing small,middle and large dose groups,additionally they were intravenously dripped with Xuebijing injection 50 mL, 2 times a day,100 mL,2 times a day and 100 mL,4 times daily respectively. The patients' vital signs,blood routine, interleukin-18(IL-18),procalcitonin(PCT)and C- reactive protein(CRP)were detected before treatment(0 d)and 3,7 and 14 days after Xuebijing injection,28-day and 60-day death rate were observed. Results With prolonged treatment,the body temperature,heart rate(HR),respiratory rate(RR),white blood cell count(WBC),IL-18,PCT, CRP levels were all decreased in each group(all P<0.05),and on 14 days they reached the valley;when compared with middle,small dose Xuebijing groups and control group,in the Xuebijing large dose group they were decreased the most significantly over the same period〔temperature (℃):36.22±0.55 vs. 36.55±0.99, 36.87±1.05, 36.93±1.07,HR(beats/min):82.11±8.99 vs. 93.45±10.02,109.02±11.44,113.13±10.34,RR(breaths/min):15.48±6.45 vs. 17.33±6.94,20.35±7.19,22.34±6.67,WBC(×109/L):5.35±1.37 vs. 8.35±2.98,10.54±3.22, 13.33±4.78,IL-18(ng/L):107.36±55.89 vs. 136.55±80.19,177.78±102.32,193.93±98.07,PCT(μg/L):0.33±0.19 vs. 0.66±0.22,1.62±0.44,2.78±0.34,CRP(mg/L):15.40±1.37 vs. 48.34±8.98,100.45±30.67, 131.92±49.53〕,the differences all being statistically significant(all P<0.05). The 28-day and 60-day death rate in Xuebijing large dose group were significantly lower than those in control group,Xuebijing small,middle dose groups (28-day:25% vs. 40%,40%,45%;60-day:30% vs. 45%,40%,50%). Conclusion The time for onset of therapeutic effect in large dose Xuebijing group is short,and reduce the levels of inflammatory mediators significantly, and improve the prognosis of patients with sepsis,therefore it can be used clinically.

Objective To investigate the prevalence of Kaschin-Beck disease in Wulan and Dulan counties,and to provide basic data in distribution of Kashin-Beck disease.Method In 2015,according to the Diagnosis of Kaschin-Beck Disease (WS/T 207-2010),in 18 villages of Wulan (Tongpu Town,Xiligou Town) and Dulan (Xiariha Town,Xiangride Town) counties,children aged 7-15 and adults over 16 years old who had bone and joint dysfunction underwent epidemiological,clinic and X-ray examination.Results A total of 3 800 children were examined,6 children were found with clinic syndrome of degree Ⅰ Kaschin-Beck disease,the detection rate was 0.16％.By X-ray examination,one student with Kaschin-Beck disease was from other place;other 5 students' X-ray signs were normal.A total of 222 adults older than 16 years old who had bone and joint dysfunction were examined,none of them was found with clinic syndrome of degree Ⅰ Kaschin-Beck disease,the detection rate was 0.Conclusion There is no patient with Kaschin-Beck disease in Wulan and Dulan counties,Qinghai Province.