In Pro.Fan Yongsheng’s view,the causa morbi and mechanism of systemic lupus erythematosus(SLE) is based on kidney deficiency,showing heat toxin and blood stasis; on the basis of differentiating disease and syndromes,to treat it with combination of TCM and western medicine,as well as by removing toxin,stasis and nourishing kidney,has good curative effect.

By means of introducing four medical records to elucidate Pro.Fan Yongsheng' experience that is actively applied in cooling blood to remove apthogenic heat and acting blood to remove stasis to treat stagnated heat stagnation syndrome in systemic lupus erythematosus.

[Objective]To investigate the clinical effect of saddle-shaped condylar plate in treating the fracture of the femoral intercondylar and supracondylar.[Method]Thirty-two cases with the fracture of the femoral intercondylar and sapracondylar,who were selected randomly were fixed by saddle-shaped condylar plates after reduction,of which 8 cases of patellar fractures accompanied with ipsilateral fracture of the femoral condylar.[Result]All cases were completely healed during the follow-up study of 4 months to 2 years,all cases achieved bone union in 5～9 months.[Conclusion]The design of the plate conforms to the principle of biomechanics and is good for the heal of the fracture.The saddle-shaped structure makes the plate and the cannular bone tangent as fixing so it can reduce the putrescence of the bone which is caused by pressure;The arch-bridge-shaped juncture and the mechanical bolts which are fixed across can strengthen the power of anti-bending and anti-rotation of the broken ends of the fractured bone.The stability of the fixation enables the patient do the functional exercise after the operation as soon as possible.It has definite clinical value and is worth of popularization.

Objective To develop an anatomically based method of accurate pedicle screw insertion for cervical spine fixation. Methods Forty adult cervical spine were mounted onto a specially designed jig. Parameters including height, width, length, and axis angulation of pedicles,depth of lateral fovea, height of superior posterior border of arcus vertebrae and angles of posterior articular prominance as well were mea sured with vernier calibre or goniometer either directly or radiographically. Results Lateral fovea did exist in all C2-C7 vertebrea. The pedicles of the cervical spine were wide and long enough to accept 28 mm long 3.5 mm screws. The abduction angle of the screw was 20? at the C1 level, 25? superiorly and 44? inferiorly at the C2 level, 45? to 47? from level C3 to C5, and 40? from level C6 to C7. The angle of posterior plane of articular mass was minus 2.30? at C3,it was 2.32? to 2.42? from level C4 to C6, and it was 15.22? at C7. The stability of cervical spine fixation and neurological improvement were satisfactory. Conclusion Transpedic ular screw fixation for the cervical spine is practicable. Anatomical symbol of the superior posterior border of the arcus vertebrae and the posterior plane of articular mass and the lateral fovea is easy to be exposed and identified in the operation. Transpedicular screw can be precisely inserted easily and safely for the cervical spine fixation with limited exposure and without using fluoroscopic guidance.

Objective To detect the frequency of peripheral blood CD4+ CD25high Tr and CD4+CD25lowT cells and expression of programmed death-1 ( PD-1 ) on their surface in the patients with systemic lupus erythematosus(SLE) and their clinical signifcance is analyzed.Methods The expression of PD-1 on the CD4+ CD25highTr and CD4+CD25lowT cells was examined in patients with 33 active SLE,18 inactive SLE and 38 healthy controls(HC) by flow cytometry.Clinical manifestations and laboratory findings of SLE were collected.Patients were divided into two groups according to their disease activity.SLE disease activity index(SLEDAI) score≥10 was defined as high disease activity and ＜10 as inactivity.The percentage of CD4+ CD25highTr and CD4+CD25lowT cells and proportions of expression of PD-1 on their surface were compared between not only inactive or active SLE patients and healthy controls (HC),but also between patients with lupus nephritis and without lupus nephritis.Correlation with clincal manifestations and laboratory findings was analyzed.Results (1)The proportions of CD4+CD25highTr and CD4+CD25low T cells were significantly decreased in active and inactive SLE patients as compared with HC.(2)The percentage of CD4+ CD25lowPD-1 +Tr and in active and inactive SLE patients were higher than HC.The proportions of CD4+CD25lowPD-1+ T cells were significantly increased in HC and inactive SLE patients as compared with inactive SLE patients.(3) The proportions of CD4+CD25highPD-1+Tr in SLE patients with nephritis were higher than those in patients without nephritis.But the percentage of CD4+ CD25 lowPD-1 + T cells was significantly decreased in SLE patients with nephritis as compared those without nephritis.(4)A positive correlation was observed for proportions of CD4+CD25highPD-1 + T cells with SLEDAI score.Proportions of CD4+ CD25high Tr,CD4+ CD25low PD-1 + T cells was inversely correlated with SLEDAI score.Conclusion The aberrations of proportions of CD4+CD25highpD-1+,CD4+CD25lowPD-1+,CD4+CD25high and CD4+CD25low T cells were observed in patients with SLE.The abnormality of PD-1 and PD-L1 pathway may play an important role in the function and number of Tr.

OBJECTIVE To study resistant mechanisms in a pan-resistant Pseudomonas putida strain.METHODS To detect the susceptibility of antimicrobial agents by MIC,6 aminoglycoside-modifying enzymes(AMEs) genes of 1 strain of P.putida were measured by PCR,and verified by DNA sequencing and similarity searches of DNA sequencing data banks using BLAST.RESULTS In the strain,there were 4 positive kinds of AMEs resistant genes(aac(6′)-Ⅰb,aac(3)-Ⅱ,ant(3″)-Ⅰand ant(2″)-Ⅰ).Sequence analysis revealed that the 4 PCR products were AMEs,the aac(6′)-Ⅰb was a new type(GenBank EU 137667).CONCLUSIONS The mechanisms of resistance to aminoglycosides of the pan-resistant P.putida are mainly related to 4 kinds of AMEs,the aac(6′)-Ⅰb is a new type.

OBJECTIVE To study 6 kinds of aminoglycoside-modifying enzymes (AMEs) genes in Chryseobacterium spp isolates. METHODS The isolates were identified by API20NE Gram-negative identification cards,and the susceptibility of antimicrobial agents was detected by MIC kits ( bioM?rieux ) 6 AMEs genes of 2 strains of Chryseobacterium spp were measured by PCR,and verified by DNA sequencing and sequence analysis. RESULTS In the 2 strains,2 kinds of resistant genes [aac(6′)-Ⅱ and ant(2″)-Ⅰ] were positive,and 4 kinds genes of AMEs [aac (6′)-Ⅰb,aac(3)-Ⅱ,ant(3″)-Ⅰ and aac(3)-Ⅰ] were negative.The amplicons were purified,sequenced and analyzed with BLAST 2.0 and found to be identical to aac(6′)-Ⅱ and ant(2″)-Ⅰ. CONCLUSIONS There are AMEs in Chryseobacterium spp isolates. This is the first report on AMEs genes [coexistance of aac(6′)-Ⅱ and ant(2″)-Ⅰ] in Chryseobacterium spp.

OBJECTIVE To know more clearly about the situation of Hepatitis B virus markers in clinical medical workers and take further interventional strategies to protect high risk medical workers.METHODS Hepatitis B virus markers in doctors,nurses and medical checkers who have contacted with patients′ blood,body fluid,or other occupational hazard situation,were detected by of ELISA.RESULTS Among the 587 medical workers detected,311 were with deficiency of active immunity(52.98%),196 were HBV infectors(33.39%).CONCLUSIONS Medical workers are in high risk groups of HBV infection.Medical institutions should attend to their self-protection and encourage them to take HBV vaccine to prevent iatrogenic transmission.

OBJECTIVE To study 40 kinds of resistant-related genes in a pan-resistant Pseudomonas aeruginosa.METHODS To detect the susceptibility of antimicrobial agents by MIC,40 resistant-related genes including 29 ?-lactamases genes,porin oprD2 genes,6 aminoglycoside-modifying enzymes(AMEs)genes,chlorhexidine/sulfadiazine resistant gene(qacE△1-sul1)and intergron(intⅠ1,2,3),etc,form 1 strain of P.aeruginosa were measured by PCR,and verified by DNA sequencing.RESULTS In the strain,there were positive of 6 kinds of resistant-related genes(blaTEM,blaOXA10,aac(6′)-Ⅱ,aac(3)-Ⅱ,qacE△1-sul1 and intⅠ1),but without oprD2 genes.Twenty-seven kinds of ?-lactamases genes,4 kinds of AMEs(aac(6′)-Ⅰb,aac(3)-Ⅰ,ant(3″)-Ⅰ and ant(2″)-Ⅰ),and 2 kinds of intⅠ(intⅠ2 and intⅠ3) were negative.CONCLUSIONS The multi-resistant mechanisms of pan-resistant P.aeruginosa are mainly related to 7 kinds of resistant-related genes(blaTEM,blaOXA10,oprD2,aac(6′)-Ⅱ,aac(3)-Ⅱ,qacE△1-sul1 and intⅠ1).

Objective To explore the effects of Jiedu Quyu Ziyin Recipe (JQZR) on the apoptosis and expressions of bcl-2 and bax mRNA of peripheral-blood lymphocyte in MRL/lpr mice. Methods 80 MRL/lpr mice were randomly divided into model group,TCM group, Western medicine group and TCM and Western medicine group,20 mice in each group, meanwhile,20 Kunming mice were selected as normal group, then intragastrically administered normal sodium, JQZR apozem, prednisone suspension and JQZR apozem and prednisone suspension, 0. 5ml every time,once daily for 12 weeks respectively. At the end of the 12th week, peripheral-blood lymphocytes of every mice purified by gradient centrifugation were cultivated for 48 hours ,then the apoptosis was detected by flow cytometry. Furthermore,the expressions of bcl-2 and bax mRNA of peripheral-blood lymphocyte were detected by RT-PCR. Results At Oh or 48h,the apoptosis ratios of PBLC in normal group, TCM group,Western medicine group and TCM and Western medicine group are higher than model group and the differences are significant(P <0. 05 ,P <0. 01 ) ,while,at Oh,which are not significant within Western medicine group, normal group and TCM and Western medicine group ( P > 0. 05) ,even if which are significant between TCM group and TCM and Western medicine group or between model group and TCM and Western medicine group( P < 0. 05 ). At 48h, the differences of apoptosis ratios of PBLC are significant between Western medicine group and TCM and Western medicine group( P < 0. 05 ), and between TCM group and TCM and Western medicine group or between model group and TCM and Western medicine group which are significant, too( P < 0. 01 ). The ratios of bcl-2/bax mRNA of normal group,TCM group and TCM and Western medicine group are significantly lower than which of model group or Western medicine group ( P < 0. 05, P < 0. 05, P <0. 01 ,respectively) ,but there is no significant difference between which of model group and Western medicine group.Conclusion JQZR has adjustable effects on the apoptosis and expressions of bcl-2 and bax mRNA of peripheralblood lymphocyte in MRL/lpr Mice.