The expression of C-myc, K-ras and H-ras oncoproteins in lymphocytesand monocytes, and NK subsets were studied in 21 patients with Behcet's syndrome (BS)and 40 healthy individuals. CD16~+,57~- cells of NK from BS patients were much decreasedwhen compared to the control. The positive percentages of three kinds of oncoprotein in BS lymphocytes and C-myc and K-ras oncoproteins in BS monocytes were significantly higher thanthat of the control. Positive correlations were found not only between C-myc expression oflymphocytes and serum ?-globulin%, IgM and IgG levels in BS, but also between C-mycexpression of monocytes and ESR. Expression of K-and H-ras oncoproteins in lympho-cytes and monocytes from BS patients were found to be negatively correlated with serumIgA level. Negative correlation was also found between C-myc expression in monocytesand CD16~+,57~- subset of NK. The significance of these results and the possible correlationsbetween BS and autoimmunity were discussed. It was suggeted that autoimmunity mightbe an important mechanism underlying BS.

mbus in IVC. Interventional treatment can prolong the survival time and promote the quality of life.

AIM:To investigate the kinetics of tumor necrosis factor alpha(TNF-?)in an animal model of chronic P.aeruginosa biomembrane associated with lung infection.METHODS:Rats were challenged with 0.1 mL of PAO579(1012 CFU/L)in alginate beads or 0.1 mL of planktonic PAO579(1012 CFU/L).After challenging for 3,7 and 14 d,bacteriological and pathological features,and TNF-? expression in lung tissue were observed.RESULTS:(1)CFU/lung in alginate beads group was significantly higher than that in planktonic bacteria group(P

Objective To study the changes of insulin-like growth factor-1(IGF-1) in serum of patients with obstructive jaundice.Methods The clinical data of 20 patients with obstructive jaundice were collected and the measurement of serum TNF-?,ALT, ALP, endotoxin and IGF-1 were performed. Results The serum IGF-1 in obstructive jaundice was significantly lower than that in gallbladder stone(P

Objective To analyze the causes of recurrent hemoptysis after interventional treatment and to provide the reference to improve the interventional radiology technique in dealing with the hemoptysis.Methods From Oct.2000 to Oct.2008,repeated interventional procedure was carried out in 22 patients with recurrent hemoptysis after initial interventional treatment.After the bronchial arterial angiography and collateral branch arterial angiography were performed,embolization of diseased arteries with gelatin sponge particle,PVA particle or microcoil was conducted.Results Of 22 patients,re-canalization of the bronchial arteries was seen in 4,blood supply from additional bronchial artery other than from the already embolized bronchial artery in 3,and blood supply from the collaterals in 15.Technical success of the second interventional treatment was achieved in all the 22 cases.Bleeding ceased immediately after the second interventional treatment and no recurrent hemoptysis occurred during the follow-up period of 3 months to 4 years.Conclusion Complete embolization of all arteries related to the hemoptysis together with actively dealing with the primary disease can effectively improve the successful rate of interventional treatment for the massive hemoptysis.

Purpose To assess the efficacy and prognostic factors of alpha-fetoprotein (AFP) negative hepatocellular carcinoma (HCC) by transcatheter arterial chemoembolization (TACE). Materials and Methods 67 AFP negative HCC patients and 67 AFP positive HCC patients underwent TACE, the survival of two groups was compared, and the prognostic factors were also analyzed. Results All 134 HCC patients were followed up for 24 months, the one-year, two-year and five-year survival rate of AFP negative and AFP positive HCC patients were 86.6%, 58.2%, 31.3%; and 81.6%, 37.8%, 13.4%, respectively, with median survival time of 34.0 months and 19.0 months, respectively. The survival rate were statistically different between AFP negative and AFP positive HCC patients (P<0.001). In AFP positive group, the median survival time of patients with AFP>400 ng/ml and AFP ≤400 ng/ml were 18.0 months and 31.0 months, respectively with statistical difference (P<0.05). Univariate analysis showed that Child-Pugh classification, histological grade, tumor size, tumor number, portal vein tumor thrombus, BCLC staging and AFP level were independent risk factors influencing the prognosis of HCC patients. Conclusion AFP negative and AFP positive (AFP ≤400 ng/ml) HCC patients achieves good efficacy and prognosis by TACE, Child-Pugh classification, portal vein tumor thrombus, BCLC staging and AFP level were risk factors influencing the prognosis of HCC patients.

Objective To evaluate the efficacy and the safety of retrograde gastrorenal shunt balloon occlusion combined with percutaneous transhepatic gastric varices embolization to treat the gastric varices with spontaneous gastrorenal shunt. Methods From Nov. 2006 to Jun. 2010, retrograde gastrorenal shunt balloon occlusion combined with percutaneous transhepatic gastric varices embolization was performed on 8 patients who had gastric varices with spontaneous gastrorenal shunt. All the patients were men and the age ranged from 40 to 61 years. The balloon catheter was inserted into the spontaneous gastrorenal shunt through the right femoral vein, then percutaneous transhepatic splenic vein venograghy was performed to identify the number and morphology of gastric varices. After that gastric varices embolization was performed while the balloon catheter was dilated, which was withdrawn one day after the procedure. Results Technical success of interventional treatment was achieved in all 8 cases with no significant complications. The increase of average portal venous pressure was 5.5 cm H2O (1 cm H2O = 0. 098 kPa,preoperative 35.0 to 41.0 cm H2O,postoperative 39.0 to 45.5 cm H2O). After follow up of 1 to 46 months, no recurrence haemorrhage occurred. Conclusion Retrograde gastrorenal shunt balloon occlusion combined with percutaneous transhepatie gastric variees embolization can be safely performed and could be one of the effective choices for patients who had gastric varices with spontaneous gastrorenal shunt, which is not suitable to treat by the endoscopic sclerotherapy.

Human cytomegalovirus (HCMV) is the most common cause of congenital infection, resulting in birth defects such as microcephaly. In this study, RT-PCR and Western Blotting were performed to quantify the regulation of endogenic nerve growth factor expression in neuroglia cells by HCMV infection. The results showed that basal, endogenous NGF expression in U251 was unchanged during early HCMV infection. NGF expression is strongly down-regulated during the latent phase of infection. These results suggest that HCMV can depress the NGF expression in U251 cells.

Objective To study the expression and significance of Th17 cells from peripheral blood of patients with rheumatoid arthritis (RA). Methods Intracelluar flow cytomete detection of IL-17/IFN-γ and IL-17/IL-6 was established using anti-CD3/Anti-CD28/IL-23 as stimulators after isolation of untouched human CD4~+T cells from PBMC. There were three groups in the present study: ①healthy controls group; ② RA stable group; ③RA active group. Results The isolation of untouched human CD4~+T cells from PBMC was effective and its purity was over 90%. The percentage of intracelluar IL-17 in CD4~+ T cells from RA patients was increased significantly. Such percentage in active group (1.54±0.41) was higher than that of stable group (0.70±0.21, P<0.01) and both of them were higher than those of healthy controls (0.42±0.12, P<0.01). Under anti-CD3/Anti-CD28/IL-23 stimulation, the percentage of intracelluar IL-17 was also increased significantly(P<0.01). The porcentage of intracellular IFN-γ was similar to that of IL-17, while that of IL-6 was not significantly different. There is an correlation between IL-17 and IFN-γ or IL-6. Conclusion There is an abnormal expression of IL-17 and IFN-γ in human CD4~+T cells in RA patients, which is related to disease activity . Th17 cells may be used as a new marker for the assessment of RA activity.