Objective To construct the recombinant adenovirus encoding mouse wild type FGFR3 cDNA. Methods Mouse FGFR3 cDNA obtained from MoFR3/SV was subcloned into plasmid pBluescript KS and further cloned into plasmid pAdTrack-CMV. The plasmid (pAdTR3) was transferred into BJ5183 cells which contained the adenovirus plasmid (pAdeasy-1) to produce recombinant adenovirus vector encoding FGFR3 cDNA (pAdE-FGFR3). The recombinant adenovirus vector was identified and transfected into the adenoviral packaging cell HEK293 by lipofectamine 2000 to get recombinant adenovirus particles. The adenovirus was confirmed by polymerase chain reactin (PCR) and its titer was determined. Then the recombinant vector was transfected into HT29 cells. The expression of FGFR3 mRNA and protein in HT29 cells was assayed by RT-PCR and Western blotting. Results The recombinant adenovirus vector encoding FGFR3 cDNA was correctly constructed and confirmed by restriction endonuclease analysis and DNA sequencing analysis. The transfected HEK 293 cells were lysed by freeze-thawing to obtain the recombinant adenovirus in the lysate. Further, PCR product of the lysate confirmed the presence of recombinant adenovirus. The viral titer was 3?109pfu/ml. RT-PCR and Western blotting showed that pAdE-FGFR3 transfected HT29 group expressed FGFR3 higher than that of GFP controlled group. Conclusion Infective recombinant adenovirus encoding FGFR3 cDNA was successfully obtained by plasmid homogenous recombination in bacteria, and highly expressed in HT29 cells after transfection, which paves a way for studying the effect of FGFR3 in bone fracture healing.

Objective To explore the effect of fibroblast growth factor receptor 3(FGFR3)on the expressions of CbfaⅠ and Collagen Ⅰ in mesenchymal stem cells(MSCs)after Ad-FGFR3 transfection.Methods After Ad-FGFR3 and Ad-GFP were respectively transfected into mouse mesenchymal stem cells,the expression of FGFR3,CbfaⅠ and Collagen Ⅰ mRNAs and proteins were detected by RT-PCR and Western blot.Results The expressions of FGFR3,CbfaⅠ and Collagen Ⅰ were significantly higher in Ad-FGFR3 transfected MSCs than in Ad-GFP group.Conclusion FGFR3 can up-regulate the expression of CbfaⅠ and Collagen Ⅰ.

In multilayer spiral CT heart and coronary artery imaging, image data are acquired by ECG gating technology when heart beats slowest so as to restrain pulsation pseudo-image. ECG gating technology includes foresight ECG gating and retrospective ECG gating. The principle, methods and mutual image reconstruction are introduced in this paper.

ve To assess the protective effect of fentanyl on primary cultured myocardial cells against injury induced by anoxia-reoxygenation. Methods Ventricular myocardial cells enzymatically isolated and cultured in 1640 culture medium for 6 days were randomly divided into 5 groups: group A received no anoxia served as control; group B received 2 hours anoxia followed by half an hour reoxygenation; and group C, D and E received 10ng ? ml-1, 30 ng ? ml-1 and 50 ng? ml-1 fentanyl respectively before anoxia-reoxygenation. The cell viability, myocardial intracellular content of malondialdehyde (MDA) and the activities of lactic dehydrogenase (LDH) and creatine kinase(CK) were measured at the end of the experiment. Results Anoxia-reoxygenation caused dramatic decrease in cell viability, and increases in myocardial intracellular MDA content and the LDH and CK activities as compared with those in control group. Fentanyl 30 ng?ml-1 and 50 ng?ml-1 significantly attenuated the increases in LDH, CK activities and MDA content, and decrease in cell viability caused by anoxia-reoxygenation. Fentanyl 10ng?ml-1 did not produce any significant changes. Conclusions Fentanyl can produce protective effects on primary cultured cardiomyocytes against anoxia-reoxygenation injury.

Objective:To survey the antibody response to live and inactive attenuated hepatitis A vaccine immunized by gastrogavage in mice.Methods:The mice were immunized by gastrogavage with live or inactive vaccine of hepatitis virus A with or without gelatin.The sera and intestinal fluid were collected after two weeks at last immunization.The specific IgG,IgA were assayed by ELISA.Results:Both of live and inactive HAV vaccine can induce the antibody response in local and system after gastrogavage immunization,the level of antibody in serum of mice is higher than that immunized by intramuscularly injection (P

Objective To analyse the lung function parameters of the patients with chronic obstructive pulmonary disease (COPD) to explore the clinical significance of lung function in the diagnosis and evaluation of COPD. Methods The lung function of 558 patients with COPD from January 2000 to April 2003 in our hospital was retrospectively analysed by using SPSS 10 0 software. Results The average ages of the 558 patients were 57 6?9 7 years, 78 9% of which were male. Patients with grades Ⅱ and Ⅲ COPD accounted for 70 2%. There was a negative relationship between FEV 1, FEV 1/FVC and smoking index(r=-0 039,-0 305,P

Retinopathy of prematurity (ROP) is a potentially blinding retinal vascular disease that occurs in premature infants and low birth weight infants.Along with the development of perinatal medicine,the incidence of ROP has increased.If infants with ROP could get treatment in time,the disability rate due to ROP would significantly reduce.Now,the present situation and advances in treatment of ROP were reviewed.

Objective To explore if obesity is related to bronchial hyperresponsiveness in different sexuality.Methods A total of 1180 patients with provocation test positive were studied.We analysed the relation of body mass index(BMI)and FVC,FEV1,the relation of BMI and PC_20 FEV1 in all patients and in the men and the women,respectively.Results There was negative correlation between BMI and FVC,FEV_1 of the whole patients.There was also negative correlation between BMI and PC_20FEV_1 of the whole patients and the women but no correlation of the men.PC_20 FEV_1 of men was higher than that of women.Conclusion Obesity is related to the decrease of lung function and the increase of airway responsiveness,this affection is obviously in women.The airway responsiveness of women is higher than that of man.

The important function of the gastrointestinal tract in monitoring and sealing the host intestine from intruders is called the gut barrier.A variety of specific and nonspecific mechanisms are in operation to establish the host barrier,including luminal mechanism,secretion of the digestive enzymes,the epithelial cells together with tight junctions between them and the gut immune system.The pathogenesis of gut barrier dysfuntion is multifactorial.They can lead to the translocation of the intestinal bacteria.Sometimes,even the multiple organ dysfunction syndrome can happen.Nitric oxide,one of the smallest products of the cell,is known to have a very large role in diverse physiologic and pathological processes.Recent study shows its toxic metabolite,peroxynitrite(ONOO~(-)),play a major role in the intestinal mucosal injury.The purpose of this literature review is to provide a better understanding of the mechanism of the protection and injury to the gut barrier function about nitric oxide and peroxynitrite.

Objective: To investigate target organ response by recording mean arterial blood pressure (MAP) fluctuation corresponding to nerve-tract discharges from the nerve innervating acupoint of Zusanli (ST 36) in the hind limb evoked by MA in anesthetized rats. Methods: Male SD rats anesthetized with chloral hydrate were randomly divided into 3 groups which were treated with manual acupuncture (MA), injection of lidocaine followed by MA and injection of normal saline (NS) followed by MA, respectively. The right carotid artery was canulated for persistent measurement of the blood pressure and meanwhile nerve discharges from the nerve-tract were recorded for analysis with amplitude spike counts for every 5 s. Results: The results showed that there were significant nerve discharges recorded from the nerve-tract when applying MA at Zusanli (ST 36) and simultaneous decrease in the MAP, while there was no response when inserting a needle into the Zusanli (ST 36) without manipulation (P<0.05). Furthermore, the reduction of MAP during MA could be completely abolished after blockade of peripheral nerve discharges with an injection of lidocaine into the tissue around Zusanli (ST 36) but not with that of normal saline (NS). Conclusion: These results indicate that MA at Zusanli (ST 36) can elicit the peripheral nerve discharges from the nerve innervating the acupoint; such kind of nerve discharges may contain acupuncture signal regulating blood pressure via somato-cardiovascular reflex.