A gas chromatographic method was established for the analysis of fatty acid composition of oils. After methlation of the samples, the retention time of the fatty acid esters in the glass capillary coated with crosslin-ked methyl silicone was closely related to the number of carbon atoms.The reproducibity of this method was satisfactory. The relative standard deviation was 0.4-1.5%. Six kinds of vegetable oils were analyzed by this method, and the results were consistent with those reported by Canadian Cereal Association. For analysis of the free fatty acid composition of becte-ria, the results were comparable with those obtained from thin layer chroma-tography and chemical analysis.

Objective To improve recognition of radiologic manifestations, mechanism, classification and differential diagnosis with the gastric benign tumor prolapsing into duodenal bulb. Methods We retrospectively analyzed and studied gastric bengin tumor prolapsing into duodenal bulb,data 8 cases performed GI series and gastroscopic examination in our hospital which all were proved by surgery and pathology. Results Radiologic manifestations of the gastric benign tumor prolapsing into duodenal bulb included①filllng defect in the duodenal bulb and in the duodenal descending segment;②pulling mucusal bridge sign and widened pyloric channal ;③revealed smooth of tumor surface mucosa and/or niche shadowes--″bull eyes″ sign;④changed of the entire gastric shape;⑤manifestation of tumor prolapsing vs retured into stomach ;⑥distribution of the primary gastric benign tumors:gastric antrum 6 cases, gastric body and fundus varied 1 case. Pathologic diagnosis the gastric benign tumors prolapsing into duodenal bulb, leiomyoma 4 cases,lipoma and neurolemmoma varied 1 case, prolapsing into duodenal decending segment, leiomyoma 2 cases. Conclusion Radiological diagnosing signs of the gastric benign tumor prolapsing into duodenal bulb include①filling defect in the duodenal bulb or duodenum ;②pulllng mucosal bridge sign ;③widened pyloric channel;④changed of the entire gastric shape.

The principle, basis, necessity and significance of formulating the local standard of Microtu fortis as a laboratory animal were described in this paper, and the standard was compared with the relationship between this standard of Microtu fortis as laboratory animal and the existing laws, regulations of other standards of laboratory animals.The specific procedures and the degree of adoption of domestic standards and advanced foreign standards were introduced.Furthermore, the proposal and the reasons of recommendatory standards were presented.

Objective: To improve recognition of radiologic manifestations, mechanism, classification and differential diagnosis with the gastric benign tumor prolapsing into duodenal bulb. Methods We retrospectively analyzed and studied gastric bengin tumor prolapsing into duodenal bulb,data 8 cases performed GI series and gastroscopic examination in our hospital which all were proved by surgery and pathology. Results Radiologic manifestations of the gastric benign tumor prolapsing into duodenal bulb included①filling defect in the duodenal bulb and in the duodenal descending segment;②pulling mucusal bridge sign and widened pyloric channal;③revealed smooth of tumor surface mucosa and/or niche shadowes - -"bull eyes" sign; ④changed of the entire gastric shape; ⑤manifestation of tumor prolapsing vs retured into stomach;⑥distribution of the primary gastric benign tumors:gastric antrum 6 cases, gastric body and fundus varied 1 case. Pathologic diagnosis the gastric benign tumors prolapsing into duodenal bulb,leiomyoma 4 cases,lipoma and neurolemmoma varied 1 case,prolapsing into duodenal decending segment,leiomyoma 2 cases. Conclusion Radiological diagnosing signs of the gastric benign tumor prolapsing into duodenal bulb include①filling defect in the duodenal bulb or duodenum;②pulling mucosal bridge sign;③widened pyloric channel:④ changed of the entire gastric shape.

ObjectiveThe aim of this study was to establish women previously undergoing cesarean section again puerperal ways.Methods170 pregnant women who had previously undergone 1 or 2 cesarean section were studied. ResultsAmong the 170 cases, 93 cases were trial of labor. The success was 81 cases, the success rate was 87%. One patient with preuterine scar ruptured. There was no maternal death. When the repeat cesarean group was compared with the trial of labor group, in the blood loss and after delivery febrile morbidity were significantly higher in women with repeat cesarean (P＜0.05). The Apgar score of newborns was no difference (P＞0.05). ConelusionWe should control the indications for cesarean section. A trial of labor after previous cesarean is safe, less damage and can be rceommended in the majority of cases. All eligible women would allowed to deliver vaginally after previous cesarean.

Objective To study the clinical characteristic and antifungal drug sensitivity of neonatal Candida parapsilosis infections in blood and to provide reference for clinical prevention and therapy.Methods The drug resistance of Candida parapsilosis which were isolated from 8 neonatal blood specimen on the laboratory were analyzed and clinical characteristic of infections in neonates were investigated retrospectively.The blood cultured with BD9120.The fungi were isolated with Sabourand Dextrose Agar and CHROMagar colored medium and identified with API20C.The susceptibility test was then performed with FUNGUS3 micro dilution plate.Results Candida parapsilosis susceptibility results to antifungal drugs showed that 5-flucytosine≤ 4 μg/ml,amphotericin B ≤ 0.5 μg/ml,fluconazole ≤ 1 ～ 2.0 μg/ml,itraconazole ≤ 0.125 ～0.125 μg/ml,voriconazole ≤0.06 ～0.06 μg/ml.Seven cases were preterm infants(low birth weight infants or extremely low birth weight infants),one case was term infant after operation of congenital pyloric atresia.Before blood culture,all the 8 cases of Candida parapsilosis sepsis had received broad-spectrum antibiotics and intravenous nutrition.All the 8 cases had received peripherally inserted central catheter,and 3 cases had received mechanical ventilation.Four cases with fluconazole,3 cases with fluconazole and amphotericin B,1 case with fluconazole at the onset but changed VFend,all 8 cases were cured.Conclusion Candida parapsilosis has become the one of the main pathogens of neonatal infection fungal in blood of premature or low birth weight infants,which is sensitive to 5 kinds of antifungal drugs in vitro susceptibility test.Early detection and antifungal therapy can improve the prognosis.

Objective To evaluate the distribution of Schmorl's nodes (SN) in patients with low back pain or radiculopathy,and to analyze the correlation between SN and degeneration of lumbar intervertebral discs.Methods In 2012,1024 patients with low back pain or sciatica were examined by magnetic resonance (MR) scan and plain film.There were 448 males and 576 females,with an average age of (54.3 ± 12.7) years (range,22-90).The features distribution of SN in lumbar endplate on age,sex,segment and the type and grade of intervertebral disc degeneration were analyzed retrospectively.Results Among 5120 lumbar intervertebral segments of the 1024 patients,295 (28.8％) cases and 532 (5.2％) endplates were involved with SN,302 located in the cranial and 230 in the caudal endplate.According to percent prevalence per lumbar segment,L1,2 was the most common level (29.1％),followed by L3,4 (23.7％) and L2,3 (21.1％).The incidence of SN was positively correlated with elder age,but not with sex,body weight,height,or body mass index.SN occurred more often in bulging,extrusion,Modic changes,spondylolisthesis and osteophyte comparing with normal disc or protrusion or high intensity zone.The distributions of the grade of intervertebral disc degeneration were significantly different between groups with and without adjacent SN.Intervertebral discs with adjacent SN were more degenerated than those without adjacent SN.The number,size and volume of SN were associated with the degrade of intervertebral discs degeneration.SN was divided into two types,i.e.,acute edematous SN and non-acuteSN,according to the signal type on T1-and T2-weighted MR images,and the degeneration of the corresponding intervertebral discs was more severe in the latter than the former group.Conclusion SN occurred more often in the upper lumbar spine and cranial endplate in patients with low back pain or radiculopathy.SN were correlated with elder age and the degeneration of lumbar intervertebral discs.The number,size,volume and signal type on MR images of SN impacted on its correlation with lumbar intervertebral disc degeneration.

Objective To investigate causes and treatment strategy of cage retropulsion after lumbar interbody fusion,in particular the correlations between the injury of the posterior part of endplate cranially adjacent to fusion and cage retropulsion.Methods Data of 7 patients with cage migration after lumbar interbody fusion from August 2011 to July 2014 in our hospital were retrospectively analyzed.There were 4 males and 3 females,aged from 45-74 years (average,53.6 years).All patients took X-ray and CT scans examinations 3 d postoperatively,and they were followed up at 3,6,12 months after operation.The characteristics of the patients and the injury of the endplate adjacent to fusion were analyzed to identify possible risk factors of cage retropulsion.Moreover,corresponding managements were performed and outcomes were recorded.Results All 7 cases of cage retropulsion occurred within 3 months post-operatively (rang,15-67 d).Posterior part of the endplate superiorly neighboring to fusion was found injured on the decompression side in all cases with cage retropulsion,and that led to an increase in the posterior intervertebral space height at the fusion segment.Other factors,including pear-shape type endplate,and improper operative manipulations may add the risk of cage retropulsion.These 7 patients complained mild back pain or no back pain,and received corresponding treatments including bed rest,activity limitation,and oral administration of anti-inflammatory analgesics.The clinical symptoms and degrees of cage retropulsion were not aggravated during follow-up.Conclusion The injury of the posterior margin of end-plate superiorly neighboring to fusion on the decompression side which caused increased posterior intervertebral space height may be key to postoperative cage retropulsion.Prevention of possible iatrogenic injury to the endplate may be effective to reduce the risk of cage retropulsion.

Objective Applying color Doppler ultrasonic test on the first dorsal metatarsal arteries,to acquire relevant Doppler bloodstream parameters,and in the end,to explore the clinical value of this approach.Methods Applying GE-LOGIQ-400 color Doppler high-frequency ultrasonic test on the first dorsal metatarsal arteries of 100 diabetes patients,contrasting to those of 60 normal persons,observing the origination,trend,smooth of bloodstream,angioplerosis Status,analyzing bloodstream spectrum(including the interior diameter of blood vessel,spectrum status,bloodstream speed,drag index,erc.),and contrasting the resuits.Results The first dorsal metatarsal arteries of diabetes patients have narrower interior diameter,faster bloodstream speed,higher drag index than those of non-diabetes patients.Conclusion The high-frequency color Doppler ultrasonography can provide objective clinical evidence on the test of the first dorsal metatarsal arteries diseases.