A gas chromatographic method was established for the analysis of fatty acid composition of oils. After methlation of the samples, the retention time of the fatty acid esters in the glass capillary coated with crosslin-ked methyl silicone was closely related to the number of carbon atoms.The reproducibity of this method was satisfactory. The relative standard deviation was 0.4-1.5%. Six kinds of vegetable oils were analyzed by this method, and the results were consistent with those reported by Canadian Cereal Association. For analysis of the free fatty acid composition of becte-ria, the results were comparable with those obtained from thin layer chroma-tography and chemical analysis.

Objective To improve recognition of radiologic manifestations, mechanism, classification and differential diagnosis with the gastric benign tumor prolapsing into duodenal bulb. Methods We retrospectively analyzed and studied gastric bengin tumor prolapsing into duodenal bulb,data 8 cases performed GI series and gastroscopic examination in our hospital which all were proved by surgery and pathology. Results Radiologic manifestations of the gastric benign tumor prolapsing into duodenal bulb included①filllng defect in the duodenal bulb and in the duodenal descending segment;②pulling mucusal bridge sign and widened pyloric channal ;③revealed smooth of tumor surface mucosa and/or niche shadowes--″bull eyes″ sign;④changed of the entire gastric shape;⑤manifestation of tumor prolapsing vs retured into stomach ;⑥distribution of the primary gastric benign tumors:gastric antrum 6 cases, gastric body and fundus varied 1 case. Pathologic diagnosis the gastric benign tumors prolapsing into duodenal bulb, leiomyoma 4 cases,lipoma and neurolemmoma varied 1 case, prolapsing into duodenal decending segment, leiomyoma 2 cases. Conclusion Radiological diagnosing signs of the gastric benign tumor prolapsing into duodenal bulb include①filling defect in the duodenal bulb or duodenum ;②pulllng mucosal bridge sign ;③widened pyloric channel;④changed of the entire gastric shape.

Objective: To improve recognition of radiologic manifestations, mechanism, classification and differential diagnosis with the gastric benign tumor prolapsing into duodenal bulb. Methods We retrospectively analyzed and studied gastric bengin tumor prolapsing into duodenal bulb,data 8 cases performed GI series and gastroscopic examination in our hospital which all were proved by surgery and pathology. Results Radiologic manifestations of the gastric benign tumor prolapsing into duodenal bulb included①filling defect in the duodenal bulb and in the duodenal descending segment;②pulling mucusal bridge sign and widened pyloric channal;③revealed smooth of tumor surface mucosa and/or niche shadowes - -"bull eyes" sign; ④changed of the entire gastric shape; ⑤manifestation of tumor prolapsing vs retured into stomach;⑥distribution of the primary gastric benign tumors:gastric antrum 6 cases, gastric body and fundus varied 1 case. Pathologic diagnosis the gastric benign tumors prolapsing into duodenal bulb,leiomyoma 4 cases,lipoma and neurolemmoma varied 1 case,prolapsing into duodenal decending segment,leiomyoma 2 cases. Conclusion Radiological diagnosing signs of the gastric benign tumor prolapsing into duodenal bulb include①filling defect in the duodenal bulb or duodenum;②pulling mucosal bridge sign;③widened pyloric channel:④ changed of the entire gastric shape.

The principle, basis, necessity and significance of formulating the local standard of Microtu fortis as a laboratory animal were described in this paper, and the standard was compared with the relationship between this standard of Microtu fortis as laboratory animal and the existing laws, regulations of other standards of laboratory animals.The specific procedures and the degree of adoption of domestic standards and advanced foreign standards were introduced.Furthermore, the proposal and the reasons of recommendatory standards were presented.

ObjectiveThe aim of this study was to establish women previously undergoing cesarean section again puerperal ways.Methods170 pregnant women who had previously undergone 1 or 2 cesarean section were studied. ResultsAmong the 170 cases, 93 cases were trial of labor. The success was 81 cases, the success rate was 87%. One patient with preuterine scar ruptured. There was no maternal death. When the repeat cesarean group was compared with the trial of labor group, in the blood loss and after delivery febrile morbidity were significantly higher in women with repeat cesarean (P＜0.05). The Apgar score of newborns was no difference (P＞0.05). ConelusionWe should control the indications for cesarean section. A trial of labor after previous cesarean is safe, less damage and can be rceommended in the majority of cases. All eligible women would allowed to deliver vaginally after previous cesarean.

Objective To evaluate the distribution of Schmorl's nodes (SN) in patients with low back pain or radiculopathy,and to analyze the correlation between SN and degeneration of lumbar intervertebral discs.Methods In 2012,1024 patients with low back pain or sciatica were examined by magnetic resonance (MR) scan and plain film.There were 448 males and 576 females,with an average age of (54.3 ± 12.7) years (range,22-90).The features distribution of SN in lumbar endplate on age,sex,segment and the type and grade of intervertebral disc degeneration were analyzed retrospectively.Results Among 5120 lumbar intervertebral segments of the 1024 patients,295 (28.8％) cases and 532 (5.2％) endplates were involved with SN,302 located in the cranial and 230 in the caudal endplate.According to percent prevalence per lumbar segment,L1,2 was the most common level (29.1％),followed by L3,4 (23.7％) and L2,3 (21.1％).The incidence of SN was positively correlated with elder age,but not with sex,body weight,height,or body mass index.SN occurred more often in bulging,extrusion,Modic changes,spondylolisthesis and osteophyte comparing with normal disc or protrusion or high intensity zone.The distributions of the grade of intervertebral disc degeneration were significantly different between groups with and without adjacent SN.Intervertebral discs with adjacent SN were more degenerated than those without adjacent SN.The number,size and volume of SN were associated with the degrade of intervertebral discs degeneration.SN was divided into two types,i.e.,acute edematous SN and non-acuteSN,according to the signal type on T1-and T2-weighted MR images,and the degeneration of the corresponding intervertebral discs was more severe in the latter than the former group.Conclusion SN occurred more often in the upper lumbar spine and cranial endplate in patients with low back pain or radiculopathy.SN were correlated with elder age and the degeneration of lumbar intervertebral discs.The number,size,volume and signal type on MR images of SN impacted on its correlation with lumbar intervertebral disc degeneration.

Background and purpose: Human epididymis protein 4 (HE4) has been suggested to be a novel biomarker of epithelial ovarian cancer (EOC). The present study aimed to evaluate and compare HE4 with the commonly used marker, carbohydrate antigen 125 (CA125) and imaging examinations in detecting the recurrence of postoperative EOC. Methods:In this study, 92 patients with the recurrence of postoperative EOC were analyzed retro-spectively. The treatments method included the secondary operation (48 patients) and chemotherapy (44 patients). The sensitivity of CA125, HE4 and CT/MRI examinations in detecting the recurrence of postoperative EOC were analyzed and correlated with surgical pathology and clinical follow-up results. Results:The sensitivity of CA125 and HE4 were 58.7%and 61.9%with no signiifcant difference (P>0.05). The diagnostic sensitivity of CA125 combined with HE4 (80.4%) was statistically higher than that of 2 tumor marks being applied independently (P>0.05). The diagnostic sensitivity of CT/MRI examinations (88.0%) was statistically higher than that of CA125 and HE4 (P<0.05), and no signiifcant difference was observed between CT/MRI and combined CA125 and HE4 (P>0.05). The highest diagnostic sensitivity (97.8%) was attained by combining of CA125, HE4 and CT/MRI examinations, which was statistically high-er than that of CA125 combined with HE4 (P<0.01), and CT/MRI examinations (P<0.05). Conclusion:Serum HE4 is an effective tumor marker in detecting the recurrence of postoperative EOC as well as serum CA125. HE4 and CA125 may improve the diagnostic sensitivity statistically. The highest diagnostic sensitivity was attained by combining of CA125, HE4 and CT/MRI examinations, which is the suitable strategy in screening the patient of postoperative EOC.

Objective To analyze the pathogen distribution and drug resistance of candida isolated from children with blood infections in our hospital,and to provide reference for clinical effective prevention and treatment.Methods The blood specimens of pediatric patients were collected between January 2009 and December 2015,and were cultured using BacT/ALERT 3D and BD9140 instruments.The candida were separated with Sobaurandps agar culture medium,and identified with chromogenic medium,API 20CAUX test strips or VITEK-2 compact YST card.The minimal inhibitory concentration of 5 drugs were determined by ATB FUNGUS 3 system.Results In 176 cases,92 strains (52.3％) were from neonatal ward,and 46 strains (26.1％) were from PICU.In newborn group,85 strains were isolated from premature,which contained the low and very low birth weight infants (37 strains),pneumonia(20 strains),neonatal respiratory distress syndrome(9 strains).In PICU,the strains were commonly isolated from children with severe infection.Among 176 strains of candida,71 strains (40.3％) were C.albicans,62 strains (35.2％) were C.parapsilosis,16 strains(9.1％) were C.glabrata,9 strains(5.1％) were C.tropicalis,and 18 strains(10.2％) belonged to other candida.Conclusion Candida blood infections can happen at all age of chlidren.The most common strains detected from blood were C.albicans,followed by C.parapsilosis.Most of these strains are susceptible to antifungal drugs,such as fluconazole,except C.glabrata.The sensitive rates to commonly used antifungal drug are more than 93％.The selection of antifungal drugs should be based on the species of strains.

Objective To study the clinical characteristic and antifungal drug sensitivity of neonatal Candida parapsilosis infections in blood and to provide reference for clinical prevention and therapy.Methods The drug resistance of Candida parapsilosis which were isolated from 8 neonatal blood specimen on the laboratory were analyzed and clinical characteristic of infections in neonates were investigated retrospectively.The blood cultured with BD9120.The fungi were isolated with Sabourand Dextrose Agar and CHROMagar colored medium and identified with API20C.The susceptibility test was then performed with FUNGUS3 micro dilution plate.Results Candida parapsilosis susceptibility results to antifungal drugs showed that 5-flucytosine≤ 4 μg/ml,amphotericin B ≤ 0.5 μg/ml,fluconazole ≤ 1 ～ 2.0 μg/ml,itraconazole ≤ 0.125 ～0.125 μg/ml,voriconazole ≤0.06 ～0.06 μg/ml.Seven cases were preterm infants(low birth weight infants or extremely low birth weight infants),one case was term infant after operation of congenital pyloric atresia.Before blood culture,all the 8 cases of Candida parapsilosis sepsis had received broad-spectrum antibiotics and intravenous nutrition.All the 8 cases had received peripherally inserted central catheter,and 3 cases had received mechanical ventilation.Four cases with fluconazole,3 cases with fluconazole and amphotericin B,1 case with fluconazole at the onset but changed VFend,all 8 cases were cured.Conclusion Candida parapsilosis has become the one of the main pathogens of neonatal infection fungal in blood of premature or low birth weight infants,which is sensitive to 5 kinds of antifungal drugs in vitro susceptibility test.Early detection and antifungal therapy can improve the prognosis.