Objective To study and discuss the effect of double hydrogen artesunate on Mcl-1 expression and its inducing effect on cancer cell apoptosis in the patients with cholangiocarcinoma.Methods Bile duct cancer cell lines QBC939 preserved in our hospital from June 2010 to December 2014 were randomly selected and divided into the control group and observation group for conducting experiments.The cells were cultured by using the conventional cultivation and double hydrogen artemisinin culture.Then the Mcl-1 expression and apoptosis of cancer cells were performed the statistical analysis and comparison.Results Statistical comparison showed that the expressions of MCL1-001 and-MCL1 201 at 12,24,48 h in the observation group were significantly higher than those in the control group,the comparison between groups were statistically significant (P<0.05).MCL1-002 expression had little difference between at 12 h and 24 h (P>0.05),but which at 48 h in the observation was significantly higher than that in the control group,the difference was statistically significant(P<0.05).And the mortality rate at 6,12,24,48,72 h in the observation group was significantly higher than that in the control group,the difference was statistically significant (P<0.05).Conclusion Double hydrogen artemisinin has obvious up-regulation effect on Mcl-1,moreover can effectively induces bile duct cancer cell apoptosis.

Objective To evaluate the effect of endothelin A receptor antisense oligodeoxynucleotides (ETAR-ASODN)on the growth of human prostatic stromal cells. Methods Primary cultured prostatic stromal cells were derived from patients with benign prostatic hyperplasia (BPH).The cells of passages 4～6 were routinely used for this study after identification.ETAR-ASODN at the concentrations of 5,10 and 15 ?mol/L were added into the culture cells with lipofectin, and cell proliferation was measured by MTT assay. The expression of ETA receptor was tested by 125 I-ET-1 radioligand banding assay. Results MTT assays showed a significant decrease in cell proliferation in stromal cells after 5,10 and 15?mol/L ETAR-ASODN were added with the A 540 values being 0.304?0.082,0.296?0.008 and 0.194?0.061,respectively.The proliferative activity was significantly decreased compared with control group ( P

Objective To investigate the change of levels of activated Nuclear Factor ?B (NF ?B) in the blood of patients with acute coronary syndrome (ACS) and its significance Methods Seventy six patients were divided into four groups: control group,stable angina pectoris group (SAP), unstable angina pectoris group (UAP), and acute myocardial infarction group (AMI) NF ?B was measured with ELISA Results The level of activated NF ?B was (0 61?0 35) ?g in control group and (0 59?0 39) ?g in SAP group, and (1 12?0 10) ?g, (1 41?0 18) ?g, (1 18?0 13) ?g, (0 82?0 18) ?g in UAP group and (1 28?0 14) ?g, (1 69?0 41) ?g, (1 55?0 45) ?g, (0 89?0 06) ?g in AMI group at 0～12 h, 12～24 h, 24～48 h, and 1 w time intervals respectively The levels of activated NF ?B were higher in UAP and AMI groups than that in control group or SAP group ( P

Objective To investigate the changes in gastrointestinal motility and cholinergic nervous system in the gastric antrum and intestine of rats with cirrhosis. Methods 20 Wistar rats were randomly divided into control group and cirrhosis model group. The changes in gastrointestinal motility of rats were assessed by Dextran blue-2000 as an indicator; the cholinergic nerves in antro-jejunal myoenteric plexus were observed with acetylcholinesterase histochemical staining and analysed with a computer. Results Compare with control group, the gastrointestinal motility of rats was markedly retarded (P

Objective To evaluate the efficiency of prevention of Oncomelania snail dispersal with the mode of leading water from middle level of water body by the culvert pipelinet in the electric pumping station. Methods The snails were detected in the floats by riverhead and the irrigation areas, and the snails were hold up by the block in the exit of pump. The snail investigation was carried out in the irrigation system areas and the results were compared between the study area and the control area. Results The snails were discovered in the floats of riverhead of the electric pumping station. However, there were no any snails in the exit of pump after the block snail treatment and no any snails in the irrigation areas, either. There were snails in the control areas Conclusion The mode of leading water from middle level of water body by the culvert pipelinet is very effective for the prevention of Oncomelania snail dispersal.

Objective To study the proliferation and apoptosis and investigate the expression of Indian hedgehog (Ihh),parathyroid hormone-related peptide (PTHrp),smoothened (Smo) protein and mRNA in the cultured rat primary chondrocytes exposed to different doses of NaF.Methods The third generation articular chondrocytes of neonate rat were cultured in vitro and treated with 0 (control),5,10,20 and 40 mg/L of fluoride.The proliferation activities of cells at different times (24,48 and 72 h) were tested by Thiazolyl Blue Tetrazolium Bromide (MTT).The apoptosis rate was determined by flow cytometry.The expressions of protein and mRNA of Ihh,Smo and PTHrp at 48 h were determined by Western blotting and semi-quantitative RT-PCR,respectively.Results After exposed to 5 mg/L of fluoride for 24,48 and 72 h,the proliferation rates were significantly increased [(1.17 ± 0.07)％,(1.20 ±0.06)％,(1.16 ± 0.08)％] compared with those of control group [(1.10 ± 0.08)％,(1.13 ± 0.08)％,(1.15 ± 0.08)％],but the proliferation activity at 48 and 72 h in 40 mg/L group [(0.72 ± 0.11)％,(0.68 ± 0.04)％] was significantly lower than those in control group (all P ＜ 0.05).Compared with the control group,apoptosis rate of cartilage cell in fluoride treatment group increased gradually [(1.47 ± 0.05)％,(19.87 ± 3.03)％,(25.30 ± 1.28)％,(45.73 ± 4.63)％,F =123.328,P ＜ 0.01].Western blot analysis and RT-PCR results showed that the Ihh,PTHrp,Smo mRNA and protein expression increased in the fluoride groups at 48 h (Ihh protein:0.77 ± 0.08 vs.0.98 ±-0.07,1.23 ± 0.06,1.37 ±0.07,1.34 ± 0.07;PTHrp protein:0.68 ± 0.04 vs.0.89 ± 0.05,0.83 ± 0.05,1.29 ± 0.05,1.16 ± 0.08;Smo protein:0.37 ± 0.01 vs.0.64 ± 0.06,0.67 ± 0.03,0.96 ± 0.06,0.69 ± 0.06;Ihh mRNA:0.77 ± 0.05 vs.0.98 ± 0.05,1.09 ±0.05,1.27 ± 0.03,1.46 ± 0.06;PTHrp mRNA:0.67 ± 0.07 vs.0.97 ± 0.05,1.07 ± 0.08,1.37 ± 0.05,1.45 ± 0.05;Smo mRNA:0.45 ± 0.03 vs.0.63 ±-0.04,0.71 ± 0.05,0.81 ± 0.01,1.00 ± 0.02,all P ＜ 0.05).Conclusions Low doses of fluoride can promote the proliferation of chondrocytes cultured in vitro,and high doses of fluoride can promote the apoptosis of chondrocytes cultured in vitro.The expression of Ihh signaling pathway RNAs and proteins of the cartilage cells are increased following increased levels of fluoride.The results suggest that fluorine has activated the Ihh signaling pathway in chondrocytes and promoted the proliferation and apoptosis processes which might be involved in chondrocytes injury.

Objective To improve the purifying method of Jo-1 antigen from rabbit thymus used for detection of anti-Jo-1 antibody by dot-blotting immunoassay(DB).Methods The rabbit thymus glands were cut into pieces,homogenized and extracted by PBS.Total protein was precipitated by acetone to get acetone powder(RTAP).The RTAP was solved in PBS and separated by an by anti-Jo-1 IgG affinity column.Results 5～7 g RTAP was obtained from 100g rabbit thymus glands.There was 19%～24% of protein in RTAP.Jo-1 antigen was enriched around 1900 folds through affinity chromatography,with 2.5% recovery of antigenic activity.In this preparation,there were several bands on SDS-PAGE,but only one band about 50 ku,reacted with anti-Jo-1 antisera on immunoblotting.Dot-blotting also showed that the antigen only reacted with Jo-1 antisera.The purified Jo-1 antigen was not stable for long time,but the antigenic activity could maintain for a long time when there was MgCl2 in the solution.Conclusion Affinity chromatography was a simple and easy method for purifying Jo-1 antigen from rabbit thymus.The antigen purified by affinity chromatography could meet the requirement for detecting Jo-1 antibody bydot-blotting.

Objective To evaluate the cost-benefit of the project with the mode of collecting water from middle layer of water body by the culvert pipelinet.Methods The cost-benefit analysis was carried out by the way of benefit-cost ratio for the project with the mode of collecting water from middle layer of water body by the culvert pipelinet without indirect cost and indirect benefit,and according to the price of 2005.Results The direct costs was 34 thousand yuans in the project with the mode of collecting water from middle layer of water body by the culvert pipelinet.The saved costs of prevention and treatment were 1011 thousand yuans,the net benefit was 977 thousand yuans and the net benefit-cost ratio was 28.7∶1 following the project implemented.Conclusion The investment is small but the benefit is high in the project of collecting water from middle layer of water body by the culvert pipelinet.The technique has the notable worthiness of applications.

Nisin, produced by several strains in the growth process of Lactococcus lactis, is a natural antimicrobial polypeptide.Now, Nisin has served as an effective and safe food additive extensively used in food industry in many countries and regions because of its excellent antimicrobial activity.However, the current production of Nisin is largely fermented by lactobacillus and its industrialized production still can not meet enormous market needs, therefore establishing reasonably high-yield Nisin strains is of great significance.This review mainly summarizes the development pathway of molecule based on the functional expression of Nisin biosynthetic genes and regulation of gene expression, and also the study status on high Nisin-producing strains which provides practical foundation for further study on expected strains as well as some useful guidance for large-scale industrialized production of Nisin.

BACKGROUND:Rehabilitation training equipments play an important role in the rehabilitation treatment. Because of poor muscle strength and joint mobility in patients, we must guarantee the safety of rehabilitation training equipments.
OBJECTIVE:To design a new suitable man-machine interface that ensures patients can use rehabilitation equipments and even parts of fitness equipments safely.
METHODS:Through user experience research, we found the flaws of the existing rehabilitation equipment. Depending on the principles of ergonomics, we designed a new man-machine interface for upper limb exercise through survey and computer-aided design.
RESULTS AND CONCLUSION:The new man-machine interface program achieves the rapid wear and discharge between patients and rehabilitation training equipment, and importantly, it can automatical y separate people from the equipment when the patient's body discomforts or equipment failure appears. What’s more, this man-machine interface can be promoted to other fitness equipments. As a result, rehabilitation training for patients wil be more convenient.