Objective This study is to examine the effect of different breathing patterns (abdominal respiration,natural respiration)on skin temperature(TEMP),skin conductivity(SC) and heart rate variability (HRV) in college students.Methods We use single blind trial in the study,and randomly select thirty students,including fourteen male and sixteen female,aged 19.6±0.77 years,and then randomly divide them into two groups:the intervention group (abdominal respiration group,6 males and 9 females) and the control group(natural respiration group,8 males and 7 females).In the study three phases are included:baseline phase(five minutes),test phase(fifteen minutes),recovery phase(five minutes).Data of above indicators was collected at 3-5 min,14.5-19.5 min,and 23-25 min,respectively,during the study.Results (1) There are no significant difierences of all three indicators between the two groups in the baseline phase(P<0.05).(2)The SC of the test phase in the intervention group is lower than in the control group (P=0.05),while the TEMP and HRV of the two groups show no significant differences(P>0.05).(3)There are no significant differences of TEMP and the HRV in the recovery phase between the two groups(P>0.05),except the SC (P<0.05).Conclusion No significant difference is observed between the influences of abdominal and natural respiration on the emotion-related biological indicators.

Objective To evaluate protective function of ischemia preconditioning for myocardium during percutaneous transluminal coronary angioplasty (PTCA), using cardiac troponin I (cTnI) as myocardial injury marker. Methods One hundred and fifty patients with coronary artery disease (CAD) undergoing PTCA were divided into two groups: Group Usual Cure (G-UC), including 120 cases, and Group Ischemia Precondition (G-IP), including 30 cases. Serum cTnI were measured before and 6, 12, 24, 48 and 72 hours after the procedure respectively. The relative factors were analyzed. The cardiac events were followed-up.Results The serum cTnI levels of 29 cases elevated in G-UC, while those of 2 cases elevated in G-IP. There was statistical difference on elevated cTnI levels between the two groups (P

Objective To investigate the change of levels of activated Nuclear Factor ?B (NF ?B) in the blood of patients with acute coronary syndrome (ACS) and its significance Methods Seventy six patients were divided into four groups: control group,stable angina pectoris group (SAP), unstable angina pectoris group (UAP), and acute myocardial infarction group (AMI) NF ?B was measured with ELISA Results The level of activated NF ?B was (0 61?0 35) ?g in control group and (0 59?0 39) ?g in SAP group, and (1 12?0 10) ?g, (1 41?0 18) ?g, (1 18?0 13) ?g, (0 82?0 18) ?g in UAP group and (1 28?0 14) ?g, (1 69?0 41) ?g, (1 55?0 45) ?g, (0 89?0 06) ?g in AMI group at 0～12 h, 12～24 h, 24～48 h, and 1 w time intervals respectively The levels of activated NF ?B were higher in UAP and AMI groups than that in control group or SAP group ( P

Objective To evaluate the efficacy and safety of transplantation of autologous bone marrow mesenchymal stem cells (BMSCs) and mononucleus cells (BMMNCs) for treating coronary heart disease. Methods Ten patients who had suffered from coronary heart disease with myocardial infarction, were selected. In 7 patients BMSCs+BMMNCs were transplanted immediately following PCI and 3 patients only accepted transplantation of BMSCs+BMMNCs without PCI. Autologous BMSCs+BMMNCs were isolated and cultured for 2-3 weeks. And (0.9～3.5)?106 BMSCs+1.6～6.1?106 BMMNCs were transplantated through intracoroary way. Cardiac functions were determined by 2-D echocardiography、 technetium- 99m methoxyisobutylisonitrile ( 99m Tc-MIBI) and ECG Holter monitor before and 6 months after the procedure. Results Left ventricular ejection fraction (LVEF) was significantly increased by 10.5% (4.0%-18.0%) and left ventricular diastolic diameter(LVDd) reduced by 2.2 mm (-4 mm-8 mm) and neither obvious arrhythmia nor complication was observed during the 6-12 months′ follow up in all the 10 patients. Conclusion The preliminary study showed that in the patients who suffered from coronary heart disease complicated with myocardiac infarction, transplantation of BMSCs+BMMNCs could improve cardiac function and cardiac perfusion without significant complication and arrhythmia during the 6-12 months′ follow-up.

Objective To study the ameliorative effect on postinfarction heart failure of Ad_5-HGF (adenovirus-mediated human hepatocyte growth factor) in swine models. Methods Totally 12 young swine were randomly divided into 2 groups which were: Ad_5-HGF group (treatment group) and null- Ad_5 group (control group). At four weeks and seven weeks after left anterior descending coronary artery ligation in swine hearts, coronary angiography and Gate cardiac perfusion imaging were performed to evaluate the degree of collateral artery growth (Rentorp), cardiac perfusion, and heart function. The expression of HGF was examined by ELISA. Immunohistochemistry was performed to illustrate the angiogenesis of SMA~+ vessels. Results (1) In comparison with the null- Ad_5 group, high expression of human HGF was observed in the myocardium of the Ad_5-HGF group. (2) The improvement in LVEF was more obvious in the Ad_5-HGF group than the control group in 7 weeks. Cardiac perfusion was significantly improved in the Ad_5-HGF group. (3) In the Ad_5-HGF group, growth of collateral blood vessels and also the number of SMA~+ vessels per mm~2 were significantly greater. Conclusion (1) High expression of human HGF is observed in the myocardium through intracoronary transfection. (2) HGF can improve collateral artery formation and ameliorate heart function. (3) HGF can increase the number of vessels that are coated with SMC.

50%.Myocardial infarction(MI) models were created by ligating the distal left anterior descending artery in swines(n=18).Either BM-MSCs cells(5?106/mL)(n=6) or Ad5-HGF(4?109 pfu)(n=6) were transfused into infracted area via noninfarction-related artery at four weeks after MI.IMDM fluid was injected into the noninfarction-related artery in the control(n=6).Gate cardiac perfusion imaging was performed at four and seven weeks after LAD ligation respectively to evaluate the heart function and cardiac perfusion.Morphologic and histologic characteristics of the hearts were also studied.Results(1) New vessels firnation was found around the infarction area in all the three groups.By means of immno-histological staining,the density of capillaries and vessels with function in the BM-MSCs group and the Ad5-HGF group were 102.4?8.6/mm2 and 105.3?7.7/mm2,as well as 52.1?4.1/mm2 and 66.0?3.3/mm2 respectively.Both vessel density were higher than those of the control(55.5?4.7/mm2 and 16.4?3.5/mm2,P

Objective This study investigated into the relationship between single nucleotide polymorphisms(SNPs)of thrombospondin-1 gene(TSP-1)and acute coronary syndrome(ACS).Methods From Nov.2003 to Sep.2005,fragment of exon thirteen in TSP-1 gene from 323 cases of ACS and 273 subjects without coronary heart disease were analysed by polymerase chain reaction and restriction fragment length polymorphism,and sequence analysis was done for confirmation in the First Affiliated Hospital of Nanjing Medical University.Results Of the 596 subjects participating in the study,only 6 of the heterozygotes and none of the homozygotes were detected for the 700S allele.The prevalence of the 8831G in the healthy controls was less frequent than those of the west populations(0.4%vs 11%,P

Objective To study the effect application,method,time, and indwelling double J internal tube in the operation of upper urinary tract.Methods The double J of tubes were used as internal drainage and internal stent for 1～2 months in the operation of upper urinary tract in 104 patients with nephrolithiasis, ureteral calculi,PUJ obstruction,megaloureter syndrome and bladder tumor.Result No complications such as wound infection,leakage of urine occurrence after operation.Conclusions The double J tubes had the dual functions of internal sent and internal drainage. The double J tubes can be indwelled easily and safely.

Objective To evaluate the possible effect of percutaneous transluminal coronary angioplasty(PTCA) on myocardial injury. Methods Serum cTnI and CK MB levels were measured in 173 patients with coronary artery disease undergone PTCA before and 6, 12, 24, 48 and 72 hours after the procedure. Cardiac events during follow up in these patients were recorded. Results Serum cTnI level was increased after PTCA in 42 patients, remained normal in 84, and was over baseline level before and after the procedure in 47. Serum CK MB level was above baseline before and after the procedure in one patient and increased in 10. Compared with normal cTnI group, elevated cTnI was related to total balloon inflation time, total pressure, number of dilation and stents deployed, contrast medium dose and occurrence of angina during balloon inflation ( P 0.05). Conclusion cTnI was more sensitive and specific than CK MB in identifying minor myocardial injury during PTCA. This injury was related to the intensity of PTCA, but not enough to make worse influence on overall outcome.

Objective To study the angiogenic effects of adenovirus mediated human vascular endothelial growth factor 165 and human angiopoietin-1 (Ad 5-VEGF 165 , Ad 5-Ang-1) in rat models of hindlimb ischemia. Methods Rat models of hindlimb ischemia were established by ligation and peeling off rat′s femoral arteries. Ad-VEGF 165 and Ad-Ang-1 were intramuscularly transferred into the rat ischemic hindlimbs. The expression of VEGF 165 and Ang-1 were examined by Western blotting. Immunohistochemistry was performed to illustrate the effects on rat ischemic muscles after gene transferring. Results (1) Western blotting showed a high expression of VEGF 165 and Ang-1 in the ischemic hindlimb muscle transferred with Ad 5-VEGF 165 and Ad 5-Ang-1 VEGF 165 respectively. (2) There was no significant difference between groups on 7 days after the transfer. The capillary to muscle fiber ratio was significantly higher in the treating groups than that in control groups and were significantly higher in VEGF 165 +Ang-1 group than in VEGF 165 or in Ang-1 groups on day 14th and day 21th. (3) Many structured vessels surrounded by a layer of smooth muscle cells were found in Ad-VEGF 165 and Ad-VEGF 165 +Ad-Ang-1 groups, the number of SMA + vessel per muscle fiber was obviously higher than those in control groups. (4) Enormous cells positive for BrdU appeared in treated muscles in Ang-1, VEGF 165 , VEGF 165 +Ang-1 groups and many of them were positive for C-Kit, an antigen expressed by pluripotent marrow stem cells. Some C-Kit+ cells were incorporated in sites of neovascularization. Conclusion (1) Vascular endothelial growth factor 165 and angiopoietin-1 can promote neovascularization in rat models of hindlimb ischemia and the angiogenic effect is much more significant in Ad-VEGF+Ad-Ang-1 group. (2) VEGF 165 can increase the number of vessles that are coated with smooth muscle cells which shapes are similar to arterioles. (3) Not only angiogenic effect but perhaps vasculogenic effct also contribute to the neovascularization.