Objective To study the relationship between matrix metalloproteinase-9 in syncytiotrophoblast and placenta pathological change of fetal growth restriction. Methods The placental samples were collected from 30 patients with FGR and 30 normal pregnant women who delivered in our hospital from January 2001 to September 2001. The tissue sections were dealt with HE stain and PAS stain and then observed by microscope to find the morphological change. 5 another samples from each group were observed by 500H model electron microscope. Immunohistochemical analysis was employed to demonstrate the positive expression of the MMP-9 in syncytiotrophoblast of FGR placenta. Results There are significantly pathological change in 22 placentas of FGR and abnormal rate is 73.33%. The count of stroma fibrosis and fibrinoid necrosis of villi(63.3%), villous vascular decrease and gore(70.0%), villous syncytial knots(43.3%), cytotrophoblasts increase(50.0%) were more significant in the placenta of FGR group than that of normal group(6.7%,10.0%,3.3%,13.3%)(P

Objective To investigate the role of vascular cell adhesion molecule 1 (VCAM 1) in pathogenesis of pregnancy induced hypertension (PIH) complicated by fetal growth restriction (FGR). Methods The placenta samples were collected from 30 pregnant women with PIH complicated by FGR, 28 FGR patients without PIH and 30 normal pregnant women. Immunohistochemical analysis was used to demonstrate the positive expression of VCAM 1 in placenta vascular endothelium and syncytiotrophoblast of these samples. Results In placentas of PIH complicated by FGR (80%),expression of VCAM 1 in the decidual vascular endothelium was significantly higher than those without PIH (25%) and normal placentas (15%)( P

50%.Myocardial infarction(MI) models were created by ligating the distal left anterior descending artery in swines(n=18).Either BM-MSCs cells(5?106/mL)(n=6) or Ad5-HGF(4?109 pfu)(n=6) were transfused into infracted area via noninfarction-related artery at four weeks after MI.IMDM fluid was injected into the noninfarction-related artery in the control(n=6).Gate cardiac perfusion imaging was performed at four and seven weeks after LAD ligation respectively to evaluate the heart function and cardiac perfusion.Morphologic and histologic characteristics of the hearts were also studied.Results(1) New vessels firnation was found around the infarction area in all the three groups.By means of immno-histological staining,the density of capillaries and vessels with function in the BM-MSCs group and the Ad5-HGF group were 102.4?8.6/mm2 and 105.3?7.7/mm2,as well as 52.1?4.1/mm2 and 66.0?3.3/mm2 respectively.Both vessel density were higher than those of the control(55.5?4.7/mm2 and 16.4?3.5/mm2,P

Objective To investigate the pharmacodynamics of rocuronium in infants with obstructive jaundice. Methods Thirty infants aged 1 to 4 months without neuromuscular diseases or renal dysfunction were divided into two groups with 15 cases each. The patients in group A were diagnosed with biliary atresia and scheduled for the kasai operation, and those in group B were with normal liver function and scheduled for abdominal surgery as the controls. Anestheisa was induced with propofol 2 mg/kg and fentanyl 3 g/kg. Rocuronium 0. 6 mg/kg was given and neuromuscular block was recorded with acceleromyography. Results Comparing with group B, The onset time of rocuronium was significantly longer in group A than that in group B[(81±35) s vs (51±28)s](P< 0. 05). There were no significant differences between the two groups in the maximum depression time [(49±15) min vs. (48±12) min]or the clinical duration of action[(76±20) min vs. (71±14) min]. Conclusion The onset time of rocuronium is prolonged in infants with obstructive jaundice.

Objective]To investigate the thought of manipulating The Three Blood Statsis Removal Decoctions(Taohe Chengqi Decoction, Di Dang Decoction, Di Dang Pills) of ZHANG Zhongjing.[Method]Collect and analyse the explanations of the scriptures involving the Three Blood Statsis Removal Decoctions of ShangHanLun interpreters from past to nowadays, combine with the theory said in Huang Di Nei Jing, knowledge of exegetics and personal view, in order to summarise the original intention of manipulating the Three Blood Statsis Removal Decoctions by ZHANG Zhongjing. [Result] The main purpose of Taohe Chengqi Decoction is to purge heat, since heat is the main reason of stagnation of blood. It applies to the preliminary stage of combination of heat and blood clot. The main purpose of Di Dang Decoction is to attack the rigid blood-heat stagnation in lower-energizer, it is applied to the late stage of combination of heat and blood clot. Di Dang Pill is the milder prescription of Di Dang Decoction, which is applied to the milder situation of blood-heat combination. [Conclusion]Comparing the Three Blood Statsis Removal Decoctions,“ Superficial”and“ deep”words should be used to differentiate the application of Taohe Chengqi Decoction and Di Dang Decoction, while“ light”and“ severe”words should be used to differentiate that of Di Dang Decotion and Di Dang Pills.

Objective:To establish the emergency medical equipment rental center, not only can reduce the repeated purchase for medical equipment, but also can achieve resource sharing, reduce equipment maintenance cost, and improve utilization efficiency.Methods: To establish emergency medical equipment rental center, which adopted lease mode and centralized management mode to use equipment. Department of medical engineering managed this center and this department is responsible to ensure every clinical department can use equipment by lease method.Results: The center has optimized the resource allocation of hospital, obviously reduced equipment input cost, improved utilization rate of medical facility since it were established. This mode provided reliable service for clinical departments.Conclusion: The established rental center of emergency medical equipment has changed the traditional management mode to a new feasible mode which can improve centralized management for medical devices, reduce waste for medical resource and achieve resource sharing.

Objective: To research the application of perspective imaging of intense laser proton beam in tumor cells, experi-ments are carried out on an ultra-intense picosecond pulsed laser facility, and the results of perspective imaging on biological samples by high energy proton beam, which is excitated among the interaction of picosecond laser with solid target, is showed. Methods: The laser energy is 12 J, the pulse width of laser is 1.5 ps, the laser focal spot diameter is 15 μm, the coefficientf or the off-axis paraboloid lens is 1.5. The target used in the experiment is made by coating a CH film with 1 μm on the back of the 15 pan-thick Cu. The distance between the proton source (laser spot) and the ant sample is 7 mm (i.e. the object distance), the distance between the sample and the RC film is 63 mm (i.e. the image distance). The detector is a stack of RCF or CR-39 films. Results: The ant is taken as the sample, a high energy proton beam, which is excitated as above on the rear surface of target, makes a 2-dimensional perspective imaging of an ant with high contrast, nanometer spatial resolution and density reso-lution. Conclusions: The result provided possibility of the application of perspective imaging of intense laser proton beam in tumor cells.

Exosomes are extracellular nanovesicles secreted by a variety of cell types such as cardiomyocyte, hepatocytes, and stem cells. They carry specific sets of mRNA, microRNA, and proteins, which play a role in intercellular communication in almost each physiological and pathological process. Exosomes, which are released after tissue cell injury, can initiate repair/regeneration responses by triggering inflammation and active fibroblast, and finally lead to tissue fibrosis. However, exosomes released by stem cells can retard tissue fibrosis by enhancing cell survival and reducing apoptosis. In this paper, we reviewed the research progress in the relationship between exosomes and tissue fibrosis.

Objective To study the ameliorative effect on postinfarction heart failure of Ad_5-HGF (adenovirus-mediated human hepatocyte growth factor) in swine models. Methods Totally 12 young swine were randomly divided into 2 groups which were: Ad_5-HGF group (treatment group) and null- Ad_5 group (control group). At four weeks and seven weeks after left anterior descending coronary artery ligation in swine hearts, coronary angiography and Gate cardiac perfusion imaging were performed to evaluate the degree of collateral artery growth (Rentorp), cardiac perfusion, and heart function. The expression of HGF was examined by ELISA. Immunohistochemistry was performed to illustrate the angiogenesis of SMA~+ vessels. Results (1) In comparison with the null- Ad_5 group, high expression of human HGF was observed in the myocardium of the Ad_5-HGF group. (2) The improvement in LVEF was more obvious in the Ad_5-HGF group than the control group in 7 weeks. Cardiac perfusion was significantly improved in the Ad_5-HGF group. (3) In the Ad_5-HGF group, growth of collateral blood vessels and also the number of SMA~+ vessels per mm~2 were significantly greater. Conclusion (1) High expression of human HGF is observed in the myocardium through intracoronary transfection. (2) HGF can improve collateral artery formation and ameliorate heart function. (3) HGF can increase the number of vessels that are coated with SMC.

0.05). The NAA/Cr and NAA/Cho in the Group A were significantly higher than those in the Group B (t=4.235, P=0.011; t=2.832, P=0.031), and the Cho/Cr in the Group A was significantly lower than that in the Group B (t=-2.323, P=0.042). Conclusions ~1H magnetic resonance spectroscopy can improve the diagnostic accuracy of glioma.