Aim To investigate the changes of serum metabolism after the treatment of DOA and DOO on myocardial ischemia reperfusion ( MI/R ) injury in rats, and to explore the pathogenesis of MI/R injury and drug action mechanism. Method The serum samples of Sham group, Model group, DOA group and DOO group of rats were acquired, gas phase time of flight mass spectrometry ( GC-TOF-MS) was applied to analyze the metabolic profiles of the samples. After da-ta preprocessing, they were processed into SIMCA 14. 1 software for multivariate statistical analysis. Results By principal components analysis ( PCA ) , partial least squares analysis ( PLS-DA) and orthogonal partial least squares analysis ( OPLS-DA ) , the Model group and Sham group were obviously separated, the drug in-tervention group and Model group were separated and close to Sham group. The therapeutic effect of DOO and DOA on MI/R injury in rats was proved. The ex-perimental results identified 13 endogenous biomark-ers, which were related to the glucose metabolism,lipid metabolism and amino acid metabolism pathway. Con-clusion DOA and DOO may protect the MI/R injured rats by regulating the glucose metabolism, lipid metab-olism and amino acid metabolism pathway.

Objective To investigate the ecological habit and characteristics of Trichobilharzia cercariae in the Huaihe river system. Methods During June of 2002-May of 2003, Radix auricularia snails were collected monthly in Yaohe Fishery of the branch of the Huaihe River. The cercariae, released from the positive snails and collected by Shade Drop Bottle, were examined by direct press. Ducklings were infected by cercariae with different ages and examined for the infection by miracidia hatching method.Results The results showed a typical seasonal fluctuation in infection rate of the snails, i.e. the cercariae detection rate was 0.81% in July, 0.65% in June, 0.07%-0.26% in April, May, August, September and October respectively, with a significant difference (? 2=26.73, P

In vivo Mammalian Bone Marrow Micronucleus Test is included in the standard battery genotoxicity testing,with great application prospects in medicine,public health,food and drug safety evaluation fields.Establishing standardized experimental methods and conditions in GLP condition and accumulating a certain range of background data could effectively ensure the reliability of the test system,and also provide strong basis to support the experimental data.We herein summarized the background data of mouse and rat bone marrow micronucleus tests performed from 2007 to 2015,to expound the standardized data collection method for rodent animal bone marrow micronucleus test.

OBJECTIVE: To explore the influence of negative emotion on the professional identity of nurses in Guangdong Province and the moderating role of social support during COVID-19 pandemic. METHODS: A convenience sampling method was used to select 436 nurses in Guangdong Province as study subjects. The Depression-Anxiety-Stress Scale, Nurse Professional Identity Scale and Perceived Social Support Scale were used to investigate the negative emotion, professional identity and social support of these nurses. RESULTS: The median, the 25 th and 75 th percentile scores of the negative emotion total scores of nurses was 10.0(3.0,17.8). The total scores of professional identity and social support were(106.6±16.9) and(56.9±13.2), respectively. The professional identity and social support were negatively correlated with negative emotion(P<0.01), while the professional identity was positively correlated with social support in these nurses(P<0.01). The negative emotion of nurses can negatively predict their professional identity, and social support plays a moderating role in it.CONCLUSION: The higher the degree of social support, the less negative emotional impact on professional identity of nurses during COVID-19 pandemic. Nurses should be given more social supports.

The objective of this study was to elucidata the correlation of 16-detector row CT features with the complications and anatomic-pathology of the disease in patients with idiopathic pulmonary fibrosis (IPF). The 16-detector row CT appearances and predominant anatomic distribution were analyzed in 38 cases of IPF. Of the 14 patients (36.8%) with honeycomb lung, 10 (71.4%) had honeycomb appearances predominantly distributed in the peripheral zone of the middle and lower lobes, particularly in the basal segments of the lower lobes. 11 patients (78.6%) had the clinical duration of honeycomb lung for more than 3 years. The interlobular interstitial thickening was seen in 16 cases (42.1%), the interlobular septa thickening in 20 cases (52.6%); and the thickened bronchus-vascular bundle in 9 cases (23.7%). The above-mentioned three kinds of CT features observably distributed in the peripheral zone of the middle and lower lobes, in the basal segments of the lower lobes, and in the lower lobes and middle lobe of right lung in the middle of the zone, respectively. In 15 patients (39.5%), ground-glass opacity was predominantly distributed in the peripheral zone of lung and in the basal segments of the lower lobes; and the clinical duration lasted less than 1 year was seen in 10 cases (66.7%). These results indicated that some characteristic manifestations with pathologic variation and predominant anatomic distribution of IDF can be shown on 16-detector row CT.
Adult ; Aged ; Female ; Humans ; Idiopathic Pulmonary Fibrosis ; diagnostic imaging ; pathology ; Male ; Middle Aged ; Tomography, Spiral Computed ; methods

OBJECTIVE:To establish a method for simultaneous determination of fatty acids in Isaria cicadae,Cordyceps militaris and C.sinensis,and to compare the difference of the contents of fatty acids among above medicinal herbs.METHODS:GC-MS method was adopted.Chromatographic condition:the determination was performed on TG-5MS gas phase capillary column with carrier gas of nitrogen at the flow rate of 1.2 mL/min.The inlet temperature was 290 ℃ by splitlesssampling;valve opening time was 1 min,and volume of sample was 1 μL.Mass spectrometry condition:the ion source is an electrospray ionization source.The temperatures of ion source and transmission line were 280 ℃,the initial temperature of the chromatographic column was 80 ℃ (gradient elution),ionization voltage was 70 eV.Solvent delay time was 5 min,and scan mass range was m/z 30-400.RESULTS:The linear ranges of tetradecanoic acid,pentadecanoic acid,palmitic acid,palmitoleic acid,heptadecanoic acid,heptadecenoic acid,docosahexaenoic acid,methyl oleate,linoleic acid,arachidonic acid,eicosenoic acid,diolefinic acid,eicosatrienoic acid,heneicosanoic acid,behenic acid,tricosanoic acid,lignoceric acid were 1.400-44.520 μg/mL(r=0.999 8),2.091-93.721 μg/mL(r=0.999 7),3.146-85.856 μg/mL(r=0.998 2),1.664-61.444 g/rnL(r=0.998 7),1.773-64.983 g/mL(r=0.999 5),1.781-68.421 μg/ mL (r=0.999 7),1.706-55.606 μg/mL (r=0.999 8),1.439-47.989 μg/mL (r=0.999 6),1.738-66.908 μg/mL (r=0.999 6),2.086-94.206 μg/mL(r=0.999 5),1.356-44.966 μg/mL(r=0.999 4),1.444-56.814 μg/mL(r=0.999 7),1.375-52.335 μg/mL(r =0.999 8),1.512-60.312 μg/mL(r=0.999 5),1.450-59.760 μg/mL(r=0.999 7),1.427-58.757 μg/mL(r=0.999 1),1.269-58.109 μg/ mL(r=0.999 3),respectively.The limit of quantitation was no more than 1 764.71 μg/mL,and the limit of detection was no more than 529.42 μg/mL.RSDs of precision,stability and reproducibility tests were all lower than 2.0％.The recoveries were 84.87％-108.93％ (RSD ranged 0.19％-2.23％,n=6).There were 17 fatty acids in C.militaris,16 fatty acids in Ⅰ.cicadae and 16 fatty acids in C.sinensis.The contents of unsaturated fatty acids in above medicinal herbs were higher than that of saturated fatty acids.The content of fatty acids in artificial cultivated Ⅰ.cicadae was mostly higher than other medicinal herbs.COCLUSIONS:The method is simple,accurate,stable and reproducible.It can be used for simultaneous determination of fatty acids in I.cicadae,C.militaris and C.sinensis.Above 3 kinds of medicinal materials.From the perspective of fatty acid content,the quality of artificial cultivated I.cicadae is best.

Workplace spirituality, as a powerful indicator of workplace outcomes, plays an important role in stabilizing nursing talents and improving nursing quality. This article aimed to summarize the overview, measurement tools, related factors and strategies of workplace spirituality from the field of healthcare, so as to promote nursing managers ′ understanding of workplace spirituality and provide a reference for improving the nursing environment.

Objective To study the clinical application effect of X-ray computed tomography (DR) and spiral CT in the diagnosis of emergency thoracic and abdominal trauma. Methods A total of 174 patients with emergency thoracic and abdominal trauma admitted in our hospital from November 19, 2016 to November 19, 2017 were enrolled in this study. All patients underwent DR and spiral CT examinations. The pathological diagnosis coincidence rate between the two diagnostic methods and the satisfaction with the diagnostic methods between the two groups was compared. Results In terms of pathological diagnosis, the coincidence rate of spiral CT in subcutaneous emphysema, rib fractures, scapular fractures, clavicle fractures, pulmonary contusion, pleural effusion, pneumothorax, atelectasis, mediastinal emphysema, liver injury, spleen injury, kidney injury, pancreas injury, celiac hemorrhage, mesentery laceration and diaphragmatic injury was 100. 00％. Except for pulmonary contusion, mediastinal emphysema, hematoma and kidney injury and pancreatic injury, the pathological diagnosis coincidence rate of spiral CT in the other chest trauma was higher than that of DR diagnosis, and the difference was statistically significant (P＜0. 05). The patients' satisfaction score of spiral CT diag nosis was significantly higher than that of DR diagnosis(P＜0. 05). Conclusion The spiral CT in the diagnosis of emer gency thoracic and abdomen trauma is better, which can improve the clinical diagnosis of coincidence rate, and is worthy of further recommendation.

Objective To analyze the diagnostic value of multi-slice spiral CTin patients with emergency chest pain. Methods 81 patients with chest pain admitted in the Department of Emergency in our hospital from October 27, 2016 to October 27, 2017 were investigated. All patients underwent multi-slice spiral CTexamination. The examination results of multi-slice spiral CTexamination were compared with those of conventional coronary angiography. At the same time, the patient's heart rate and satisfaction during different examinations was compared. Results There were 64 patients with coronary artery stenosis, 9 patients with aortic dissection, 8 patients with pulmonary embolism were detected by multi-slice spiral CT, accounting for 79. 01％, 11. 11％ and 9. 88％ respectively. There were 64 cases of coronary artery stenosis, 10 cases of aortic dissection and 7 cases of pulmonary embolism detected by routine coronary angiography examination, accounting for 79. 01％, 12. 35％ and 8. 64％, respectively. There was no significant difference between the results of the two examination methods (P＞0. 05). There was no significant difference in the level of heart rate between patients with multi-slice spiral CTand those with conventional coronary angiography(P＞0. 05). The patient's satisfaction with multi-slice spiral CTwas 96. 30％, which was significantly higher (P＜0. 05). Conclusion For patients with emergency chest pain, the detection of multi-slice spiral CTis similar to that of routine coronary angiography in the cause of the disease, and has little influence on the patient's heart rate, which is an ideal method for clinical diagnosis of chest pain.

Objective:To analyze the influential factors for inpatient care among residents in Shanghai and to provide evidence for health policy.Methods:Data were obtained from the Fifth National Health Services Survey,the independent variables were selected based on the framework of Andersen Model,and the two models were used to analyze the determinants-relevant hospitalization probability and the cost among residents in Shanghai.Results:The hospitalization rate among permanent residents over 18 years old was 6.1％ in 2013 in Shanghai.For the hospitalized population,its average expenses (median) were 10 000.00 yuan.Gender,age,education,marriage,smoking,drinking,exercise,the travel time,family annual income,Engel coefficient and the number of chronic diseases were the determinants of hospitalization probability.Gender,age,smoking,drinking,exercise,Engel coefficient,medicaid and level of medical institution were the influential factors for the hospitalization costs.Conclusion:The utilization of inpatient care among residents in Shanghai is determined by many factors.Thus,the corresponding health policies should be formulated for different populations.