Minichromosome maintenance proteins (MCMs) are the primary control factors for eukaryotes DNA replication.MCMs play important roles in the starting place and the extending process of DNA replication.MCM2,one member of MCMs,expresses little in stationary phase while highly in proliferative and transformational phase.MCM2 can accurately reflect the cell proliferation activity and is considered as a specific maker for carcinoma and precancerous lesions.The overexpression of MCM2 is closely correlated with the genesis and development of tumors,and it maybe a good maker for early screening and prognosis assessment in many cancers and used in clinical.

Objective Through the English newspaper reading activities to create the information transmission platform based on the medium of English,so as to improve the students' ability of receiving,understanding,conversion,processing,and output information.Methods Two natural classes of seven-year program students of Grade 2011 with no difference in Englishproficiencywere selected at randomas control group(30) and the experimental group(30).And reform on reading course has been performed in the experimental class by incorporating English newspaper reading into traditional teaching routine,while the control class maintain the traditional teaching routine.After the teaching,through CET 4 test scores to evaluate the teaching effect.Independent t-test was conducted on the performances of CET 4 between the experimental class and controlled class with the level of test α=0.05.Results The control group was higher than the experimental group inthe total scoreof CET 4 and the performance oflistening and reading,and there was significant difference between the two groups(P of totalscore=0.000,P of listening score=0.002,P of reading score=0.001).But there was no statistically significant difference in writing subtests between the two groups(P=0.072).Conclusion By adopting English newspaper reading and broadcasting in class,students' overall ability of using English has been greatly enhanced,especially the listening comprehension and reading comprehension; however,there is no significant change in the development of students' writing ability due to the tasks designed.

Objective To explore the effects of interest-induced and psychological intervention in pediatric operating room nursing.Methods Totally 150 cases of children undergoing surgery in our hospital were recruited and randomly divided into the experimental group(n=75)and the control group(n=75).The experimental group received routine care as well as interest-induced and psychological intervention in anesthesia induction room.The control group only received routine care.The children's anxiety degree,anesthesia induction cooperation and parent satisfaction were measured.Results The scores of anesthesia induction cooperation and anxiety in the experimental group were significantly lower than those in the control group (P＜0.05 for both),and parent satisfaction in the experimental group was significantly higher than that in the control group(P＜0.05).Conclusion The interest-induced and psychological intervention in pediatric operating room nursing can effectively alleviate preoperative anxiety,promote anesthesia induction cooperation and increase parent satisfaction.

TCM is the treasure of Chinese culture, and the world's cultural treasure as well. 21st century is the age of information, so China has been using information technology to speed up the internationalization of TCM. With overall expanding of system engineering of TCM informatization, wisdom medicine has become a growth industry, which leads to the demand for high-end information technology talents and management talents. Based on the data of informatization talents training in TCM colleges and universities in China, this article made a statistical analysis, and carried out the related discussion on the cultivation model for characteristics and high-end interdisciplinary TCM informatization talents.

Cervical artery dissection (CeAD) is characterized by an intramural hematoma of the internal carotid artery or the vertebral artery.CeAD is the major cause of ischemic stroke in young adults.Carotid artery dissection is a major cause of stroke.Early recognition and treatment of CeAD is important for the prevention of ischemic stroke.

Objective To discuss the influence of tibolone on near and long-term postoperative effect of osteoporosis patients with bone cementless type artificial total hip replacement effect.Methods 136 patients with hip fracture by bone cementless type artificial total hip replacement were selected.According to the situation of osteoporosis,all patients were divided into osteoporosis group and control group.The osteoporosis group received tibolone treatment,followed up for 12 weeks.The intraoperative blood loss,operating time were analyzed.Enzyme-linked immunosorbent assay was used to determine serum bone alkaline phosphatase(sBAP),osteocalcin(sOC),C-terminal peptide collagen cross-linking Ⅰ type(sCTx).Harris hip score was applicated to evaluate hip function.Before and after treatment,lumbar vertebra bone mineral density(BMD) was detected by dual-energy X-ray absorption metry method.Results The intraoperative blood loss,operating time between the two groups had no significant differences(all P>0.05).The serum sBAP and sOC levels of the osteoporosis group were (28.41±6.13)U/L,(22.74±5.87)g/L,which were significantly lower than those of the control group[(35.18±7.21) U/L,(27.42±6.38)g/L],the serum sCTx level[(0.56±0.21)ng/mL]was higher than that of the control group[(0.42±0.11)ng/mL].The differences were statistically significant(tsBAP=5.36,tsOC=6.62,tsCTx=6.71,all P<0.05).On behalf of the hip joint function in the osteoporosis group was 58.82%,which was lower than 71.76% in the control group(χ2=6.78,P<0.05).The lumbar spine BMD of the control group at 12 weeks was higher than the osteoporosis group,but there was no statistically significant difference(P>0.05).Conclusion The type of bone cement after total hip arthroplasty patients with osteoporosis bone metabolism and bone mineral density in osteoporosis patients are poor in the hip joint function recovery is poorer,postoperatively for drug resistance to osteoporosis treatment can effectively avoid further loss of bone mass,it is worthy of further clinical promotion.

Objective To explore the influence of integrated Chinese and western medicine therapy on anxiety,sugar metabolism and subjective well-being for type 2 diabetes Patients associated with anxiety.Methods Use glycated hemoglobin (HbAlc),trait anxiety inventory(TAI),and Memorial University of Newfoundland Scale of Happiness (MUNSH) to evaluate the condition of 60 patients with type 2 diabetes associated with anxiety.The patients participated in the study were randomly divided into Western medicine therapy group(control group)and integrated traditional Chinese and Western medicine therapy group (experimental group),with 30 cases in each group.The control group was treated with paroxetine while the experimental group was treated with paroxetine plus Jiawei-Xiaoyaowan.6 weeks after treatment,we tested the HbAlc level.TAI score and MUNSH score of those patients.Results After 6 weeks,the TAI score of patients in both experimental group and control group have obviously decreased (before treatment:54.07±6.41 and 56.33 ±6.01,respectively; after treatment:38.89±6.70 and 45.29±6.55,respectively) and the difference was statistically significant (P＜0.05).And the TAI score of the two groups after treatment was significantly different (P＜0.05).For both experimental and control groups,the HbA1e level were not obviously changed before and after the study with P＞0.05 (before treatment:8.14±2.69 and 8.07±2.11,respectively; after treatment:8.21±2.07 and 7.92± 1.90,respectively).Comparison between groups also showed no significant difference.MUNSH score have obviously increased in experimental group (before treatment:40.49±5.22; after treatment:44.53 ± 6.28,P＜0.05).The result of comparison between the two groups after treatment shows that MUNSH score of patients in the experimental group were obviously improved compared with patients in the control group (57.56 ±6.39),and the difference was statistically significant (P＜0.05).Conclusion The integrated traditional Chinese and western medicine therapy can obviously release anxiety and enhance subjective well-being in patients with cerebral infarction associated with anxiety.

Objective:To observe the relationship between the vascular endothelial growth factor (VEGF) and the survival of flap in the rat back which received radiotherapy. Methods: Forty Wistar rats were divided into four groups. A 3×4 cm~2 full thickness dorsal flap with pedicle was prepared at the posterior end. The skin flaps were given~(60)Co radiotherapy in the 2, 4, 6 day postoperation. Group A was given of VEGF in the third, fifth, seventh day. Group B was given a high quantity of VEGF. Group C was the control of Group A,Group D was the control of Group B. Control groups received normal saline. Results: Pathological observation revealed that the survival of flap and blood vessel lumen diameter of group A were higher than that of group B(P<0.05). Group A showed remarkable difference compared with group C(P<0.01). Group B showed remarkable difference compared with group D(P<0.05). Results of fluorescence staining and SDH examination showed that three times injection had better results than one time injection. Conclusion: Three times injection of VEGF has better effect on the viability of rat skin flap than one time injection.

BACKGROUND: Nowadays, aerobic exercise has been a theme of mod ern body-building. Therefore, there are practical significance of under standing and mastering the effect of aerobic exercise on the constitution of body builder in instructing body-building practice. OBJECTIVE: To observe the effect of aerobic exercise on body composi tion, cardiore spiratory function and body figure of female college students. DESIGN: Non-randomized concurrent control experiment. SETTING: China Female College. PARTICIPANTS: The experiment was conducted from May 2003 to Oc tober 2004. 100 female college students were randomly selected from Chi na Female College and other three colleges and taken as the aerobic exer cise group. Another 100 female college students of the same age without systematic aerobic exercise were taken as the control group. METHODS: ①The course for aerobic exercise group were mainly aero bics, ballroom dancing and hexagram boxing, including 5-15 minutes of warming up, 30-45 minutes of aerobic exercise [the target heart rate in (75% maximal heart rate) exercise lasted for at least 15 minutes] and 15-20 minutes of cooling down, which were conducted for 60 minutes each time and twice a week. The exercise should be performed at 2.5 hours before supper for 1.5 years.②Subjects in the control group were not inter fered but kept in same diet and resting time as the aerobic exercise group. MAIN OUTCOME MEASURES: ①Body figure indexes: body height, body mass, chest circumference, waistline, hip circumferences.②Skinfold thickness, fat mass index and lean body mass. ③ Lung capacity, maximal breathing capacity in each minute, stroke volume, cardiac output, ejection fraction, heart rate, ejection time, mean normalized systolic ejection rate (MNSER) and left velocity of circumferential fiber shortening (LVCf). RESULTS: A total of 200 subjects, who had accomplished all tests were involved in the analysis of results. ①The waistline was smaller after exer cise in the aerobic exercise group than that in the control group, while the chest circumference and hip circumferences were bigger than those in the control group (P ＜ 0.05), and the waist-to-hip ratio was smaller than that in the control group (6.50±10.48, 70.39±11.24, P ＜ 0.05). There was no sig nificant difference in body mass index between the two groups (P ＜ 0.05). ②The skinfold thickness in rump, scapular region, thoracic region, abdom inal region as well as femoral region and region of shank were smaller after exercise in the aerobic exercise group than those in the control group (P＜0.05-0.005), and the body fat percentage was significantly smaller than that in the control group [(20.5±3.45)%, (26.5±4.58)%,P ＜ 0.005]. However, the lean body mass in the aerobic exercise group was greater than that in the control group [(41.8±9.86), (36.9±8.69) kg,P ＜ 0.01].③ After exercise, the lung capacity, maximal breathing capacity in each min utes, stroke volume, MNSER and LVCf in the aerobic exercise group were remarkably greater than those in the control group (P ＜ 0.05). CONCLUSION: ①Aerobic exercise can effectively ameliorate the body figure of female college students and coordinate three measurements with each other.②Aerobic exercise can greatly reduce the in vivo fat contents of female students and increase their lean body mass. ③Aerobic exercise can remarkably enhance the lung capacity, pulmonary ventilation volume as well as cardiac functions of blood-pumping and contraction etc. In female college students.

Objective To elucidate the clinical features, diagnosis and treatment of Aspergillus necrotizing bronchitis. Methods Medical records and investigation data from 5 eases of Aspergillus necrotizing bronchitis were retrospectively analyzed, and related literatures were reviewed. Results Five patients (3 males and 2 females) with mean age of 63.6 y were enrolled in this study. All patients had histopathologically proved necrotizing aspergillosis of the large airways. Common presentations included cough, short of breath, sputum production, fever and haemoptysis. Tracheoscopy presentations included necrosis, mucous hyperemia, focal airway constriction and neoplasm. The CT findings included bronchial wall thickening, narrowing of the bronchial lumen, which was often associated with distal obstructive pneumonia. Conclusions The clinical manifestations of Aspergillus necrotizing bronchitis are nonspecific, and histopatholagical diagnosis is essential for a definite diagnosis and can be supported by tracheoscope or imaging techniques.