ObjectiveTo investigate the selection of dilivery of pregnant women and related factors.MethodsThe selection of delivery modes for pregnant women was investigated by analyzing the associated factors of the infants registered into a computerized health care managing system from January 1,2008 to December 31,2010 at a local community of Shanghai.The information associated with delivery modes of their mothers was collected and the choice of delivery modes investigated through a standardized questionnaire by interviewing the mothers at two months after birth.ResultsThe cesarean section (C/S)rate of pregnant women residing in this local area was 58.3 ％ (224/384).In the C/S group,the percentages of delivery age ≥24 yr,white-collar workers,education level at high school and above and monthly income ≥ 3000 yuan were 73.2％ ( 164/224 ),38.4％ ( 86/224 ),79.5％ ( 178/224 ) and 80.8％ ( 181/224 )respectively.And they were all higher than those of vaginal delivery (63.1％,n =101; 32.5％,n =52;69.4％,n =111; 70.6％,n =113 ).The choices of C/S depended on the existences of maternal,fetal,social-psychological and others factors during pregnancy.Most pregnant women and families who chose C/S without medical indications were afraid of pain ( 33.9％ ) or a failure of vaginal delivery ( 30.5％ ).ConclusionsThe social-psychological factors of pregnant women are important in the choices of delivery modes.Unnecessary C/S could be reduced by improving their knowledge of delivery with meticulous prenatal health care consultations.

0.05).CONCLUSION:Domestic octreotide acetate have same effect to imported octreotide acetate in the treatment of Esophageal and gastric Varices Bleeding which is caused by portal hypertension in patients with decompensated liver cirrhosis.Stopping bleeding rate of the achieved the similar level with.

Tropane alkaloids,such as hyoscyamine (or its more stable racemate atropine) and scopolamine,are remembered as the oldest drugs in medicine with wide pharmaceutical applications clinically for their mydriatic,antispasmodic,anticholinergic,analgesic and sedative properties.Presently,the supply of tropane alkaloids are entirely rested on their isolation from plant materials,yet the low contents of tropane alkaloids in plants raise the requirement to breed new varieties of high-yield tropane alkaloids and improving the production of tropane alkaloids in hairy root cultures or plants by biotechnology has be a research focus in the field of secondary metabolism.For the past decade,there have been important progresses on biosynthesis of tropane alkaloids and their molecular biology,some new pathway genes have been identified and many achievements have also been acquired in metabolic engineering of tropane alkaloids.This review summarized the recent advances in above aspects,and the problems and orientations of future research are also discussed and proposed.

Objectives To observe the effect of inhibition of transforming growth factor (TGF)?1 on the growth and aggression of colon cancer cells by RNA interference. Methods HCT116 colon cancer cells were transfected with small interfering dsRNA (siRNA), the effect of the inhibition of TGF?1 was tested by semi-quantitative RT-PCR, and changes in cell cycle distribution were analyzed by flow cylometry, the ability of aggression was detected by soft agar colony assay. Results The inhibition of the expression of TGF?1 was significantly detected after the transfection of siRNA against TGF?1, which induced the increase of S phase by 59.0%-82. 5% and decrease in G2 phase, colony assay further demonstrated that siRNA against TGF?1 led to a significant reduction in colony formation as compared with the control group. Conclusions The present data suggested that the expression of TGF?1 was conveniently and rapidly blocked by siRNA transfection, and this may lead to the cell cycle arrest and inhibition of the growth and tumorigenesis of colon cells.

Twenty six healthy males, 60-70 years old, having the same vitamin C nutritional status detected by saturation test were selected for this study. They were divided into 4 groups and supplemented orally with various amounts of vitamin C for 15 days. The intake of each group was 59, 94, 149 and 223 ing/day per capita respectively. After comparing the vitamin C contents of 4-hour saturation test urine and early morning 1 hour fasting urine at the end of the experiment, it seems that the optimal daily requirement for the elderly is about 95 mg, and the saturated daily requirement is a little higher than 223 mg.

Objective To study the effect of matrine on proliferation and metastasis associated the abilities of human highly metastatic ovarian cancer HO-8910PM cells and its possible mechanism.MethodsThe trypan blue staining and MTT assay were used to examine the effect of matrine on proliferation of HO-8910PM cells;Transwell chamber assay was performed to determine the effect of matrine on the invasive and migratory abilities of the cells;Coomassie brilliant blue staining assay was used to detect the changes in cytoskeleton treated by matrine at different concentrations and immunocytochemical methods were used to observe the expression of Ezrin protein in HO-8910PM cell line befores and after matrine treatment.Results There was an obvious dose-dependent and time-consuming effect on the inhibition of HO-8910PM cells proliferation by matrine;1.5 g/L matrine inhibited cell proliferation significantly,for 6 h after the treatment in vitro,the invasive and migratory abilities of HO-8910PM cells were also obviously down-regulated with the inhibitory rates(41.52?10.76)% and(39.73?10.67)%,respectively;Matrine distinctly changed the cytoskeleton and up-regulated the expression of Ezrin protein in HO-8910PM cell line treated with 1.0 g and 1.5 g/L matrine for 24 h.Conclusion Matrine inhibits the proli-feration,migration,and invasion abilities of HO-8910PM cell line in vitro,which may associate with the changes of cytoskeleton and Ezrin protein expression.

Objective To discuss the advantages of balloon combined with stent-assisted steel-coil embolization in treating intracranial wide-necked aneurysms located at artery bifurcation sites. Methods The clinical data of 20 patients with intracranial wide-necked aneurysms located at artery bifurcation sites (25 aneurysms in total), who were admitted to the First Affiliated Hospital of Kunming Medical University during the period from January 2014 to May 2014 to receive balloon combined with stent-assisted steel-coil embolization treatment, were retrospectively analyzed. Among the 20 patients, 16 had a history of subarachnoid hemorrhage and 4 had no history of subarachnoid hemorrhage. A total of 25 intracranial aneurysms located at artery bifurcation were detected; the locations included basilar terminal bifurcation (n=14), middle cerebral artery (MCA) bifurcation (n=8) and internal carotid artery (ICA) bifurcation (n=3). Three dimensional DSA was performed immediately and three months after the treatment , and the manifestations were evaluated according to Raymond classification. The clinical efficacy at three months after the treatment was assessed with the modified Rankin scale (mRS). Results Three dimensional DSA performed immediately after the balloon combined with stent-assisted steel-coil embolization treatment showed that among the 25 intracranial wide-necked aneurysms located at artery bifurcation 21 belonged to Raymond gradeⅠ, 2 belonged to Raymond grade Ⅱand 2 belonged to Raymond grade Ⅲ. Follow-up DSA performed three months after the treatment revealed that 20 aneurysms were Raymond grade Ⅰ, 3 aneurysms were Raymond gradeⅡand 2 aneurysms were Raymond grade Ⅲ. Three months after the treatment the mRS score was 0 point in 17 patients and one point in one patient, and the prognosis of these patients was good; the mRS score was 4-6 points in 2 patients, and the prognosis of the two patients was poor. Conclusion For the treatment of intracranial wide-necked aneurysms located at artery bifurcation sites, balloon combined with stent-assisted steel-coil embolization has obvious advantages.

Objective To analyze the impact of rs3826392 polymorphism in MKK4 promoter on prognosis of colorectal cancer cases (CRC) receiving adjuvant chemotherapy. Methods The associations between rs3826392 genotype of 203 CRC cases receiving adjuvant chemotherapy and clinicopathologic factors,overall survival (OS), disease free survival (DFS) were analyzed retrospectively. Results No association was found between rs3826392 genotype and clinicopathologic factors (P > 0.05). TG+GG genotype had better OS (P = 0.018) and DFS (P =0.019) when compared with TT genotype. Cox multivariate model showed rs3826392 TG+GG genotype remained independent favorable factor for OS(HR = 0.389;95%CI = 0.177-0.855) and DFS(HR=0.491;95%CI = 0.271-0.890) respectively. Conclusion -1304G variant genotypes (i.e., TG+GG) in rs3826392 may be the biomarker of better prognosis in CRC receiving adjuvant chemotherapy.

Objective To comparatively study the difference between thromboelastography (TEG)and light transmission ag-gregometry (LTA)in monitoring clopidogrel effect in patients with acute coronary syndrome.Methods 68 patients with a-cute coronary syndrome,who were in hospital from February to December 2013,were enrolled in this study.They received TEG and LTA to determine platelet aggregation.Results Clopidogrel effect was (47.84±26.04)% and (45.64±20.92)%respectively by TEG and LTA.There were negative correlation between LTAADP and TEGADP(r=-0.752,P<0.001),pos-itive correlation between LTAADP and MAADP(r=0.789,P<0.001),negative correlation between TEGADP and MAADP(r=-0.820,P<0.001).Conclusion There was a good correlation between the two methods.TEG can be used to evaluate thera-peutic effect of Clopidogrel effect.

Objective To investigate the expression and activity of transforming growth factor-β1 (TGF-β1)and endothelial nitric oxide synthase(eNOS) in the cavernous vascular structural remodeling of diabetic rats.Methods 52 adult male SD rats were randomly assigned to experimental group(DM) and control group(NDM).In DM group,diabetes was induced in rats 4 days after intraperitoneal injection with streptozotocin.HbA1c was measured on 2,4,8,and 16 weeks after injection.Penile tissues were harvested.The protein expression of TGFβ1 and eNOS in situ was evaluated by the Envision immunohistochemistry.Results Compared to NDM group,the expression of the HbA1c increased significantly in DM group on the 16th week(P ＜0.01),both the penile erection rate and penile erectile times decreased significantly in DM group on the 16th week (P ＜ 0.01),and the value of eNOS decreased on the 16th week(P ＜0.01).The expression of TGF-β1 went up in DM group compared to NDM group on the 8th week (P ＜ 0.01) and sustained to the 16th week (P ＞ 0.05).In DM group,we found that TGF-β1 protein in cavernous body of penis was positively related with age in weeks(r =0.947,P ＜0.01) ; and eNOS in cavernous body of penis was negatively related with age in weeks (r =-0.945,P ＜ 0.01).Meanwhile,the expression of TGF-β was negatively related with the eNOS(r =-0.891,P ＜0.01).Conclusion Our results indicate that TGF-β1 plays an important role in the pathogenesis of diabetic erectile dysfunction(ED) and TGF-β1 inhibition may be a promising strategy to prevent development of diabetic ED.