ObjectiveTo explore the influences of parental rearing pattern and adult attachment on narcissism of college students.Methods 344 college students from two universities in Hebei Province were sampled randomly and tested by the Chinese version of EMBU,the Narcissistic Personality Inventory and the Experiences in Close Relationships Inventory (ECR) in Chinese version.Results ( 1 ) The whole score of overt narcissism correlated with paternal warming,punishment,interference,rejection and maternal warming( r =0.163,0.130,0.184,0.109,0.206),but not with adult attachment; covert narcissism correlated with paternal punishment,over-interference,rejection,over-protection,maternal over interference and protection,rejection,and punishment,and anxiety and aviodance of adult attachment positively ( r =0.135,0.169,0.210,0.123,0.220,0.194,0.215,0.183,0.269).(2) The parental reaming pattern affected overt narcissism directly,while that affected covert narcissism directly with adult attachment,and indirectly through adult attachment.ConclusionCotraditory rearing patterns between father and mother lead to overt narcissism,and negative rearning patterns lead to covert narcissism with the mediator role of adult attachment.

ObjectiveTo explore the influence of narcissism of mothers of middle school students on maternal consciousness and their rearing patterns,and to provide theoretical support for family education.Methods361 pairs middle school students and their mothers were sampled randomly and tested by middle school students' mother maternal consciousness scale and the narcissistic personality Inventory for the mothers,and the mother's subscale of EMBU for the students respectively.The data were analyzed by ANOVA,product-moment correlation and path analysis.Results 1 ) Overt narcissism had significant differences among different levels of income(F=6.501,P＜0.001 ); 2)overt narcissism was correlated positively with emotional warmth and understanding of mother and mother' s over-interference,over-protection ( r =0.121,0.126,P ＜ 0.05 ) ; covert narcissism was correlated negatively with maternal consciousness and emotional warmth and understanding of mother,and positively with over-protection,refusal and deny,and severe punishment; 3 )narcissism of mothers not only would influence their rearing patterns through maternal consciousness indirectly( β=0.319,P=0.000),but would influence that directly ( covert narcissism:β =0.281,P =0.000 ; overt narcissism:β =- 0.278,P =0.000) ; and overt narcissism had positive effect,while covert narcissism had negative effect.ConclusionNarcissism of mothers of middle school students influences maternal consciousness and rearing patterns,and effect of overt narcissism is positive,and that of covert narcissism is negative.So it is important to perfect mother's character for ameliorating their rearing pattern.

Objective To study the effect of breviscapine injection on cerebral blood perfusion in patients with hypoperfusion/decreased embolic clearance type cerebral infarction.Methods 100 patients with hypoperfusion/decreased embolic clearance type cerebral infarction in our hospital were selected and randomly divided into two groups.Both group were treated with conventional western medicines, and the experimental group was additionally treated with breviscapine injection.The plasma D-dimer (D-D), fibrinogen (Fib), systolic and diastolic blood pressure and recovery of neurological function were compared between the two groups.Results D-D and Fib levels and NIHSS score in the experimental group were significantly lower than those in the control group (P< 0.05), while the systolic blood pressure was significantly higher than that in the control group (P< 0.05).Conclusion Breviscapine injection can reduce the plasma D-D and Fib levels, increase cerebral vascular blood flow and improve the neurological function in patients with hypoperfusion/decreased embolic clearance type cerebral infarction.

Objective To analyze the clinical characteristics of spinal cord injury patients with postoperative adhesive intestinal obstruction, and summarize the clinical experience of diagnosis and treatment. Methods 35 spinal cord injury patients with postoperative adhesive intestinal obstruction in our department from August 2008 to August 2013 were reviewed. Results All of them had a history of abdominal surgery for various reasons, 22 cases received sigmoid colon pouch procedure and 13 cases received cholecystectomy. 25 cases suffered T10-L1 spinal cord injury, 10 cases suffered cervical spinal cord injury; 26 cases (74.3%) received non-operative treatment and recovered, 9 cases (25.7%) were transferred to surgery. Conclusion There is no typical clinical symptom and sign in spinal cord injury patients with adhesive intestinal obstruction after abdominal surgery, so early diagnosis is difficult, and the imaging examination is necessary. When non-operation treatment is not effective to these patients, surgical treatment should be timely carried out.

Objective To investigate the relationship of serum levels of macrophage migration inhibitory factor (MIF), matrix metallo-proteinase-9 (MMP-9), hypersensitive C-reactive protein (hs-CRP) with the severity of acute cerebral infarction (ACI). Methods 101 pa-tients with ACI were envolved and divided into 3 groups according to the scores of National Institute of Health Stroke Scale (NIHSS):mild group (group A, n=38, NIHSS score<4), moderate group (group B, n=36, NIHSS score 4-15) and severe group (group C, n=27, NIHSS score>15). Meanwhile, 44 non-ACI inpatients with atherosclerosis were included as control group (group D). Their serum MIF and MMP-9 were detected with ELISA, and hs-CRP with immunoturbidimety. Results The levels of serum MIF, MMP-9 and hs-CRP ranged from more to less as groups C, B, A and D (P<0.05). There was positive correlation of MIF with MMP-9 (r=0.301, P<0.01) and hs-CRP (r=0.309, P<0.001). Conclusion Serum MIF, MMP-9 and hs-CRP levels increased with the severity in ACI patients, which may be prediction of athero-sclerotic plaque instability.

Objective:To analyze the relationship between virulent phenotypes and the expression of antigens.Methods:The authors observed virulent phenotypes of different Shigella and its mutant by Congo red test(Pcr),contacting hemolysis test (CHT),invading HeLa cells test(Inv) and keratoconjunctivits test(Ser) and analyzed genetic background of the virulence of Shigella.Took the convalescent sera of monkeys infected with Shigella flexneri 2a to analyze the virulence associated antigens of different Shigella and enteroinvasive E.coli2 by BA immunoblot methods.Results:All virulent Shigella strains have a 120～140 MegaDalton(MD) plasmid and are Pcr(+),CHT(+),Inv(+),Ser(+).While all virulent Shigella strains are negative.All virulent strains contain four invasive plasmid antigens(IpaA?B?C?D),while nor do virulent strains.The virulence of Shigella is related not only with Ipas,but also with LPS antigen.Conclusion:The virulence of Shigella is associate with biology phenotypes of Shigella.Only with common expression of the two antigens(Ipas and LPS),the bacteria express the virulence.

Objective: To study the effects of nano red elemental selenium on liver protection, tumor inhibition and immune regulation. [WT5FZ]Methods: [WT5BZ]Models of acute liver injury induced by CCl 4, S 180 tumor growth and immune regulation were used. Results: Nano red elemental selenium could increase blood and liver Se contents, inhibit the increase of liver MDA and serum AST induced by CCl 4, decrease tumor weight and increase phagocytic ratio and NK cell activity in S 180 bearing mice, increase immune activities in normal mice. All these improvements were statistically significant (P

ObjectiveStromal cell-derived factor -1 (SDF-1 ) is closely related to the biological characteristics of breast cancer. We aimed to explore whether estrogen affected breast cancer by SDF-1. MethodsThe breast cancer cell line MCF-7 and MRC5 were chosen, and divided into three groups: the control group, the estrogen group and the estrogen + estrogen receptor blocker group. Each group was cultured with different physiological concentrations of 17-β estrogen at certain time, and the same alcohol concentration of 17-β estradiol at different time points, and then the enzyme-linked immunosorbent assay (ELISA) was used to measure the concentration of SDF-1 in culture medium, and the semi-quantitative reverse transcriptionpolymerase chain reaction (RT -PCR) was used to detect the expression of SDF-1 mRNA in each group.ResultsSDF-1 can be detected in the culture medium of both MCF-7 and MRC5 cell lines. All different concentrations of 17-β estradiol may increase the secretion of SDF-1 in MCF-7 cells. When adding 17-β estradiol to the concentration of 107mol/L, the secretion of SDF-1 reached the peak in 2 hours, which was 6 times and 2.7 times that of control group ( P ＜ 0.01 ). The effect could be ehminated by pure estrogen receptor ICI182,780. In addition, the mRNA expression of SDF-1 was consistent with the SDF-1 protein levels-l07 mol/L group. The expression of SDF-1 mRNA was higher than both that of the control group and the blocking group in 2 hours (P ＜ 0.05 ). ConclusionsIn some breast cancer cell lines, physiological concentrations of estrogen can increase the secretion of SDF- 1, and this effect is mainly achieved through the estrogen receptor. Estrogen can influence the biological characteristics of breast cancer by SDF-1.

Objective: To select the telomerase positive cancer cell lines of gastrointestinal tract and to provide a convinced methodology for future telomerase study. Methods: Fifteen cancer cell lines (carcinoma of stomach 4, of liver 6, of pancreas 2, of colon 3) were cultured and telomerase activity were detected by TRAP-ELISA. The normal hepatic cells were taken as control. Results: Thirteen cell lines were telomerase positive in the 15 lines(86.7%). Conclusion: Most of gastrointestinal tract cancer lines express telomerase, indicating the detection of telomerase activity has clinical potential.

Clinical, radiological and pathological presentations in two children with epidermal nevus syndrome were analyzed and relevant literature was reviewed.Two patients both had typical epidermal nevus and abnormal cerebral radiography, which was associated with mental retardation, epilepsy, language and movement retardation.One case was complicated with an ocular tumor.Pathological investigations of the epidermal nevus revealed papilliform proliferation in squamous epidermis.The disorder may have a systemic involvement besides cutaneous lesions, with a predilection for the central nervous system.Early diagnosis and therapy may help to improve patients life quality.