Objective:To investigate the antitumor effect of Low-Frequency Pulse Forcusd Ultrasound(LFPFU) exposure in human cell line COC1,and to clarify its probable mechanism.Methods:The growth inhibitory effect of ultrasound exposure[0.8MHz,83.6W/cm 2,pulse duration(PD) 50?s;sonication time of 10s] on COC1 cell was observed by MTT colormetric assay.Apoptosis was analyzed using flow cytometry(FCM) and terminal deoxynucleotidyl transferase-mediated dUTP-digoxin nick end labeling(TUNEL),and fluorescent microscope and electron microscope were applied to detect the morphological changes of COC1 cell.The protein expression of bcl-2 and bax affected by LFPFU were determined by FCM and immunofluorescent techniques.Results:Sonication could obviously suppress proliferation of COC1 cells surviving ultrasound exposure.The absorbance value(A 570 )of MTT decreased significantly at 12h,24h,36h and 48h after sonication as compared with control group.The hightest apoptosis rate was found to reach 23.57?4.62% by FCM,and brown-colored positive apoptotic cells were observed with TUNEL method at 6h after sonication.Typical morphological changes of apoptosis such as apoptotic body was found under fluorescent microscope and transmission electron microscope.Fluorescence index(FI) of bcl-2 decreased,and Fl of bax increased after LFPFU exposure.Conclusion:LFPFU can induce apoptosis in vitro in human ovarian cell line COC1 surviving ultrasound exposure.The bcl-2 and bax protein may be involved in the apoptotic process.

0.05).Conclusion:HepG2/Adm/nude xenograft established by HepG2/Adm cells transplantation retains the characteristics of MDR phenotype.It can provide an ideal animal model for the study of human tumor MDR.

According to Wiener's cybernetics,the author deduces the fundamental principle of negative feedback teaching and builds its model.On the basis of the model,author raises the approach of negative feedback in pathophysiology teaching.

Objective To explore an effective treatment for perimenopausal dysfunctional uterine bleeding.Methods 136 patients with perimenopausal dysfunctional uterine bleeding were received mifepristone treatment.Hemoglobin,thickness of the endometrium and sex hormones indicators,including progesterone (P),estrogen (E2),the corpus luteum generated hormone(LH),follicle hormone(FSH),prolactin(PRL) and testosterone(T),were detected before and after treatment.Results The treatment effective rate was 89.7％.After taking a course of treatment,89 cases decreased to restore normal menstrual cycle ;33 cases entered menopause after taking a course of treatment.After treatment,hemoglobin level was significantly increased compared with before treatment (t =3.899,P ＜ 0.05),and endometrial thickness significantly reduced (t =5.652,P ＜ 0.05).After treatment,the P,E2,FSH,LH levels were significantly lower than before treatment(t =5.320,4.309,3.872,3.641,all P ＜ 0.05).T,PRL levels had no significant differences.Conclusion The mifepristone treatment for perimenopausal uterine bleeding have good clinical efficacy,less adverse reactions,and worthy of clinical application.

Total hip and knee replacements, which have developed for abou t forty years, have been widely used as effective surgical treatments for severe hip and knee diseases. This article discusses the recent advance in techniques of hip and knee replacement Chiefly in the aspects of hip or knee prosthesis des ign, improvement of materials and surgical techniques. In total hip replacement, second-generation cementing technique, new types of metal-on-metal prosthesi s and minimally invasive technique have been applied to enhance the stability, r educe the wear rate and facilitate the functional recovery. In knee replacement, mobile bearing and high flexion are great advancements made in design. The comp uter-aided operational technique has greatly improved the replacement.

To investigate the effects of Carvedilol on neurohormone and heart function in patients with congestive heart failure(CHF) by double blinded, randomized clinical trial. 35 congestive heart failure patients were double blinded and randomly divided into 2 groups, Carvedilol group and placebo group. 15 healthy persons constitute the normal control group. We measured the plasma concentration of norepinephrine(NE), angiotensin (Ang) and plasma renin activity (PRA) , meanwhile left ventricular ejection fraction (LVEF) , left ventricular dimension and volume were observed before and after treatment. The plasma concentrations of NE, Ang Ⅱ and PRA were found to be higher in patients with CHF than those of healthy control group ( P

Objective:To examine the effects of different concentrations of Isoflurane on the level of surfactant protein A(SP-A) and the expression of SP-A mRNA in the lung of rats.Methods: Thirty-two male Wistar rats were randomly divided into 4 groups: control group received 40%(volume fraction) O_2 inhalation(40% O_2,n=8);0.7% isoflurane group(n=8),1.5% isoflurane group(n=8).They and 2.0% isoflurane group(n=8).They were treated with 40%O_2 with 0.7%,1.5% and 2.0% isoflurane respectively.In each group,the rats inhaled experimental gas for 8 hours,and then were put to death immediately.Morphological changes of type Ⅱ pneumocytes were observed by transmission electron microscopy.Surfactant protein content in broncho-alveolar lavage fluid(BALF) was measured with Western Blotting.The level of intracellular SP-A was examined with immunohistochemistry(IHC).To assess the relative levels of SP-A mRNA in lung tissue,reverse transcriptase-polymerase chain reaction(RT-PCR) was used with the co-amplification of the "housekeeping" gene GAPDH as internal control.Results: Morphological changes of type Ⅱ pneumocytes were distinct in 1.5% isoflurane and 2.0% isoflurane groups.Isoflurane,after three doses,significantly reduced the SP-A content in BALF.Control group,(437 112)?25 654;0.7%Isoflurane group,355 789?28 116;1.5%Isoflurane group,238 554?31 531;(2.0%) Isoflurane group,223 632?25 710(P

BACKGROUND: Hypogonadism happens gradually with body aging. Traditional Chinese medicine views that deficiency of essential qi is the chief reason for body aging. OBJECTIVE: To observe the influence of tiannianyin, the Chinese herb , on serum testosterone, superoxide dismutase (SOD) activity in testicular tissue and expression of proliferating cell nuclear antigen of spermatogenic cells in testes in subacute senile rats induced by D-galactose. DESIGN: Randomized controlled experiment. SETTING: Basic Medicine Institute of Chengde Medical College. MATERIALS: The experiment was perlormed in Basic Medicine Institute of Chengde Medical College from April to July 2003, in which, 40 adult male SD rats were employed and randomized into 4 groups, 10 rats in each .In normal group, no any management was done. In model group, medication group and negative control, D-galactosie was used for abdominal injection continuously at 200 mg (kg·d), once a day, totally for 40 days to prepare the model of subacute senile animal. In model group, no any management was done after modeling success. In medication group, gastric infusion with tiannianyin. Tiannianyin was administrated everyday after modeling success, once a day, totally for 30 days. In negative control, gastric infusion with physiological saline of same dosage was administrated after modeling success for same days. METHODS: Enzyme linked immunosorbent assay (ELISA) was used for determination of serum testosterone content, xanthine oxidase assay was used for determination of SOD activity in testicular tissue and immunohistochemical method was used to observe expression of proliferating cell nuclear antigen in rats of every group. MAIN OUTCOME MEASURES: ① serum testosterone content; ② SOD activity in testicular tissue; ③ expression of proliferating cell nuclear antigen of spermatogenic cells. RESULTS: Forty rats all entered result analysis. ① Serum testosterone content: That in model group was lower than normal group(P ＜ 0.05), and that in medication group was higher than model group(P ＜ 0.05). ② SOD activity in testicular tissue: That in model group was lower remarkably than normal group(P ＜ 0.01), and that in medication group was higher remarkably than model group(P ＜ 0.01). ③ Expression of proliferating cell nuclear antigen of spermatogenic cells: That in model group was lower than normal group(P ＜ 0.05), and that in medication group was higher remarkably than model group(P ＜ 0.05). CONCLUSION: Tiannianyin delays degeneration of reproductive function and aging of certain degree.

BACKGROUND: In follow of the gradual decrease of senile gonad function in the body, traditional Chinese medicine views that deficiency of essential qi is the chief reason of body senility. OBJECTIVE: To observe the effects of tian nian yin on serum testosterone content, superoxide dismutase activity in testicular tissue and apoptosis of testicular seminiferous cell in subacute senile rats. DESIGN: Randomized controlled experiment. SETTING: Human Anatomy Department of Chengde Medical College. MATERIALS: The experiment was performed in Institute of Basic Medicine of Chengde Medical College from April to July 2003, in which,40 SD male rats were employed and randomized into 4 groups, 10 rats in each group. In model group, medicinal group and the control, D-galactose was injected 200 mg/(kg·d) to prepare subacute senile animal model, once a day, continuously for 40 days. No any management was given in model group after modeling success. In medicinal group, gastric infusion with tian nian yin [composed of zhi heshouwu (Radix Polygoni Mutiflori Preparatea),huainiuxi (Radix Achyranthis Bidentatae), roucongrong (Herba Cistanchis),fuling (Poria), danshen (Radix Salviae Miltiorrhizae) and yinyanghuo (Herba Epimedii), it was decocted till 1.5 g/mL with raw herbs contained] was administrated once a day, totally for 30 days after modeling success. In the control, gastric infusion was administrated with physiological saline of same usage as the medicinal group after modeling success. INTERVENTIONS: In every group, enzyme linked immunosorbent assay (ELISA) was used to determine testosterone content in serum. Xanthine oxidase assay (XOD) was used to determine superoxide dismutase activity in testicular tissue and immunohistochemical method (IHC) was used to observe apoptosis of testicular seminiferous cell. MAIN OUTCOME MEASURES: ① Testosterone content in serum. ②Superoxide dismutase activity in testicular tissue. ③ Apoptosis of testicular seminiferous cell RESULTS: Forty rats all entered result analysis. ① Testosterone content in serum: Testosterone content in model group was lower than normal group [(0.52±0.15) μg/L, (1.26 0.32) μg/L, (t=3.004, P ＜ 0.05)], and that in medicinal group was higher than model group [(1.16±0.32) μg/L,(0.52 0.15) μg/L, (t=2.321, P ＜ 0.05)]. ② Superoxide dismutase activity in testicular tissue: Superoxide dismutase activity in model group was lower remarkably than the normal group [(107.22±9.33) kNU/g, (147.73±11.90) kNU/g,(t=13.339, P ＜ 0.01)], and that in medicinal group was higher remarkably than model group [(141.05±14.57) kNU/g, (107.22±9.33) kNU/g, (t=9.970,P ＜ 0.01)]. ③ Apoptosis of testicular seminiferous cell: Percentage of apoptotic seniniferous tubule and apoptotic rate of positive cell in model group were higher remarkably thannormal group respectively [(53.95 ±2.02)％compared with (34.21±2.10)％, (8.67±0.80)％ compared with (3.86±0.52)％,(x2=7.9, P ＜ 0.01, x2=5.01, P ＜ 0.05)], and those in medicinal group were lower remarkably than model group[(35.33±2.18)％ compared with (53.95±2.02)％, (4.68±0.74)％ compared with (8.67±0.80)％, (x2=7.02, P ＜ 0.01, x2=3.96, P ＜ 0.05)]. CONCLUSION: The apoptosis of testicular seminiferous cell was increased in senile modelgroup. Tian nian yin in gastric infusion decreased apoptotic rate of positive cell. It is explained that tian nian yin eliminates surplus oxygenic free radical, decreases apoptosis and improves seminiferous function in animal model.

Objective To observe the effect of neoadjuvant chemotherapy combined with tumor cells reduction and postoperative intraperitoneal perfusion chemotherapy on the efficacy and quality of life of ovarian cancer.Methods From October 2010 to October 2013 at the Department of gynecology,Taizhou Municipal Hospital,65 cases of ovarian cancer patients for the treatment of stage Ⅲ and Ⅳ were randomly divided into intervention group (n =32) and control group (n =33).Patients in the intervention group received neoadjuvant chemotherapy after tumor cell reduction cytoreductive surgery and received postoperative intraperitoneal hyperthermic perfusion chemotherapy and conventional surgery after chemotherapy.Patients in the control group received conventional tumor cell cytoreductive surgery plus postoperative chemotherapy.Surgical status,the effectiveness of treatment,and adverse reactions were compared between two groups.Moreover,survival quality score,relapse rate,and mortality differences were compared between two groups.Results For the intervention group,operation status and effective treatment were better than the control group.The proportion of adverse reactions had no significant difference between two groups.Survival quality had no statistically significant difference between before treatment and after receiving treatment.Quality of life in the intervention group was better than that of the control group.Relapse rate,and mortality rate in the intervention group was lower than the control group.Conclusions Accepted neoadjuvant chemotherapy combined with tumor cytoreductive surgery and surgery after intraperitoneal hyperthermic perfusion chemotherapy can improve the effectiveness of treatment of ovarian cancers,and improve the quality of life,with better short-term effect.