Objective To explore an effective treatment for perimenopausal dysfunctional uterine bleeding.Methods 136 patients with perimenopausal dysfunctional uterine bleeding were received mifepristone treatment.Hemoglobin,thickness of the endometrium and sex hormones indicators,including progesterone (P),estrogen (E2),the corpus luteum generated hormone(LH),follicle hormone(FSH),prolactin(PRL) and testosterone(T),were detected before and after treatment.Results The treatment effective rate was 89.7％.After taking a course of treatment,89 cases decreased to restore normal menstrual cycle ;33 cases entered menopause after taking a course of treatment.After treatment,hemoglobin level was significantly increased compared with before treatment (t =3.899,P ＜ 0.05),and endometrial thickness significantly reduced (t =5.652,P ＜ 0.05).After treatment,the P,E2,FSH,LH levels were significantly lower than before treatment(t =5.320,4.309,3.872,3.641,all P ＜ 0.05).T,PRL levels had no significant differences.Conclusion The mifepristone treatment for perimenopausal uterine bleeding have good clinical efficacy,less adverse reactions,and worthy of clinical application.

Objective:To investigate the antitumor effect of Low-Frequency Pulse Forcusd Ultrasound(LFPFU) exposure in human cell line COC1,and to clarify its probable mechanism.Methods:The growth inhibitory effect of ultrasound exposure[0.8MHz,83.6W/cm 2,pulse duration(PD) 50?s;sonication time of 10s] on COC1 cell was observed by MTT colormetric assay.Apoptosis was analyzed using flow cytometry(FCM) and terminal deoxynucleotidyl transferase-mediated dUTP-digoxin nick end labeling(TUNEL),and fluorescent microscope and electron microscope were applied to detect the morphological changes of COC1 cell.The protein expression of bcl-2 and bax affected by LFPFU were determined by FCM and immunofluorescent techniques.Results:Sonication could obviously suppress proliferation of COC1 cells surviving ultrasound exposure.The absorbance value(A 570 )of MTT decreased significantly at 12h,24h,36h and 48h after sonication as compared with control group.The hightest apoptosis rate was found to reach 23.57?4.62% by FCM,and brown-colored positive apoptotic cells were observed with TUNEL method at 6h after sonication.Typical morphological changes of apoptosis such as apoptotic body was found under fluorescent microscope and transmission electron microscope.Fluorescence index(FI) of bcl-2 decreased,and Fl of bax increased after LFPFU exposure.Conclusion:LFPFU can induce apoptosis in vitro in human ovarian cell line COC1 surviving ultrasound exposure.The bcl-2 and bax protein may be involved in the apoptotic process.

0.05).Conclusion:HepG2/Adm/nude xenograft established by HepG2/Adm cells transplantation retains the characteristics of MDR phenotype.It can provide an ideal animal model for the study of human tumor MDR.

According to Wiener's cybernetics,the author deduces the fundamental principle of negative feedback teaching and builds its model.On the basis of the model,author raises the approach of negative feedback in pathophysiology teaching.

To investigate the effects of Carvedilol on neurohormone and heart function in patients with congestive heart failure(CHF) by double blinded, randomized clinical trial. 35 congestive heart failure patients were double blinded and randomly divided into 2 groups, Carvedilol group and placebo group. 15 healthy persons constitute the normal control group. We measured the plasma concentration of norepinephrine(NE), angiotensin (Ang) and plasma renin activity (PRA) , meanwhile left ventricular ejection fraction (LVEF) , left ventricular dimension and volume were observed before and after treatment. The plasma concentrations of NE, Ang Ⅱ and PRA were found to be higher in patients with CHF than those of healthy control group ( P

Total hip and knee replacements, which have developed for abou t forty years, have been widely used as effective surgical treatments for severe hip and knee diseases. This article discusses the recent advance in techniques of hip and knee replacement Chiefly in the aspects of hip or knee prosthesis des ign, improvement of materials and surgical techniques. In total hip replacement, second-generation cementing technique, new types of metal-on-metal prosthesi s and minimally invasive technique have been applied to enhance the stability, r educe the wear rate and facilitate the functional recovery. In knee replacement, mobile bearing and high flexion are great advancements made in design. The comp uter-aided operational technique has greatly improved the replacement.

Objective:To examine the effects of different concentrations of Isoflurane on the level of surfactant protein A(SP-A) and the expression of SP-A mRNA in the lung of rats.Methods: Thirty-two male Wistar rats were randomly divided into 4 groups: control group received 40%(volume fraction) O_2 inhalation(40% O_2,n=8);0.7% isoflurane group(n=8),1.5% isoflurane group(n=8).They and 2.0% isoflurane group(n=8).They were treated with 40%O_2 with 0.7%,1.5% and 2.0% isoflurane respectively.In each group,the rats inhaled experimental gas for 8 hours,and then were put to death immediately.Morphological changes of type Ⅱ pneumocytes were observed by transmission electron microscopy.Surfactant protein content in broncho-alveolar lavage fluid(BALF) was measured with Western Blotting.The level of intracellular SP-A was examined with immunohistochemistry(IHC).To assess the relative levels of SP-A mRNA in lung tissue,reverse transcriptase-polymerase chain reaction(RT-PCR) was used with the co-amplification of the "housekeeping" gene GAPDH as internal control.Results: Morphological changes of type Ⅱ pneumocytes were distinct in 1.5% isoflurane and 2.0% isoflurane groups.Isoflurane,after three doses,significantly reduced the SP-A content in BALF.Control group,(437 112)?25 654;0.7%Isoflurane group,355 789?28 116;1.5%Isoflurane group,238 554?31 531;(2.0%) Isoflurane group,223 632?25 710(P

Objective: To investigate the relationship between peripheral blood absolute lymphocyte count (ALC) and patients with multiple myeloma (MM). Methods:We obtained clinical features and follow-up data of 102 patients with MM to analyze the prog-nostic value of peripheral blood ALC. Results:Patients with ALC>1.51×109/L were designated as group 1, whereas patients with ALC<1.51×109/L were designated as group 2. Group 1 had significantly lower age as well as concentrations of lactate dehydrogenase (LDH) andβ2-MG than group 2 (P<0.05). The overall survival (OS) of group 1 was significantly higher than group 2 (P<0.05). Sex, ALB, Du-rie-Salmon stage, isotype, subgroup, and stage according to the International Staging System in group 1 were not significantly different compared with group 2 (P>0.05). Conclusion:Lower ALC is a poor prognosis factor. ALC may be an important prognostic factor of MM.

Aim To develop a sensitive, rapid and ac-curate LC-MSn method for determination of midazolam/1′-hydroxymidazolam and their pharmacokinetic char-acteristics in rat brain. Methods SD rats received in-travenous injection of midazolam ( 5 mg · kg-1 ) via femoral vein, a probe drug of cytochrome P450 3A. The microdialysis ( MD ) samples in situ brain were collected every 8 mins at 2. 0μl·min-1 in 2. 4 hours. Analytes in brain dialysate were quantified by the pro-posed LC-MSn method. Gradient elution was performed on an Agilent Eclipse Plus-C18 column ( 2 . 1 × 50 mm, 3. 5 μm). The mobile phase consisted of 2 mmol ·L-1 ammonium acetate and acetonitrile. The analyte was detected using electrospray ionization ( ESI ) in multiple reaction monitoring ( MRM) modes. The reac-tion selected ions were 326 . 1/291 . 1 m/z for midazo-lam, 342. 1/324. 1 m/z for 1′-hydroxymidazolam and 285. 1/154. 0 m/z for diazepam as internal standard. Result The linear ranges of midazolam and 1′-hydroxymidazolam were 0 . 78~100 and 0 . 195 ~12 . 5μg·L-1 respectively. The lower limit of quantification was 0 . 2 μg · L-1 . The RSD of intra- and inter-batch precisions was less than 7 %. The RSD of accuracy was from -1 . 34 to -8 %. Conclusion This sensi-tive and rapid LC-MSn method is suitable for determi-nation of midazolam/1′-hydroxymidazolam in rat brain dialysate. MD combined with LC-MSn method may give assistance to deep and further studies of drug metabo-lism and CYP3A enzyme in brain.

Objective The aim of the study was to investigate the effects of inflammation on the biological be-haviors of the VF -MSCs and provide the theoretic basis for the repair of the vocal folds which were damaged by in-flammation .Methods The inflammatory vocal fold tissues and normal vocal fold tissues were respectively derived from the vocal cord polyp and normal tissues of the hypopharyngeal carcinoma patients .The HE staining ,masson trichrome staining and elastin van gieson (EVG) staining were performed to detect the effects of inflammation on the collagenous fiber and elastic fibers of the vocal fold lamina propria .The cell colony formation analysis and MTT cell growth curve were used to detect the effects of inflammation on the proliferation of VF -MSCs .The effects of in-flammation on the multi-directional differentiation of VF -MSCs were evaluated by inducing the VF -MSCs to dif-ferentiate into osteoblasts and lipoblasts .Results The results of masson trichrome staining and EVG staining showed that in the inflammatory vocal fold lamina propria collagen fibers became thicker and the amount of collagen fibers increased ,while elastic fibers became thinner and the amount of elastic fibers decreased .Compared with the vocal fold mesenchymal stem cell (VF-MSCs) in normal vocal folds ,VF-MSCs in inflammatory vocal folds pro-liferated more significantly ,but the osteogenic differentiation and adipogenic differentiation of VF -MSCs in inflammatory vocal folds were restrained .Conclusion Inflammation enhanced the compressive resistance ,abated the elasticity , and restrained the multi -directional differentiation of VF -MSCs .