After the practical experiences with embedded subject service for research in nursing science in Library , The First Affiliated Hospital of Chongqing Medical University was described , such as constructing and recommending resources for nursing staff , helping them to search and read related resources , providing training service of how to write and submit papers , the models of embedded subject service for research in nursing science were summarized , such as laying equal stress on construction and recommendation of resources , systematic service in the embedding processes , and tackling problems by groups in coordination .

The visual angle of active participation reference model was not scientific and its contents were not sys-tematic in the past EBM information service. A new EBM information service model was thus established by compa-ring the finding process of evidence and the transforming process ofinformation-knowledge-intelligence with the need of evidence-based decision making in clinicians as a logic starting point in order to provide the theory of what to do andhow to scientifically do for clinical librarians and clinicians, to effectively direct clinical librarians to perform EBM information service, and to help clinicians to scientifically make their decision.

Objective To observe the efficacy of acupuncture at Hegu (LI4) in treating central facial palsy due to cerebral ischemia, and to explore the dose-effect correlation and the optimal acupuncture protocol.Method Totally 150 patients with central facial palsy due to ischemic stroke were randomized into four groups to receive acupuncture at Hegu with different stimulation parameters plus the conventional integrated Chinese and Western medicine (group A, B, C, D) according to the acupuncture time and direction, and a control group (group E) only to receive the conventional Chinese and Western medicine treatment. The treatment lasted 14 d. The House-Brackmann facial nerve grading system, Toronto Facial Grading System and facial palsy grading scale were adopted as the evaluation indexes.Result After intervention, the scores of the three indexes were all significantly increased in the five groups (P<0.05), and the inter-group comparisons showed significant differences in comparing the improvement rate (P<0.05).Conclusion The study shows that acupuncture at Hegu is effective in treating central facial palsy due to cerebral ischemia, and with the inverse insertion of the needle and manipulation for 5 min at Hegu should be the optimal protocol regarding the improvement of the symptom score and effective rate.

OBJECTIVE:To strengthen quality management of drug microbiology laboratory and provide reference for relevant inspection institutions. METHODS:Taking measures from the laboratory document management,personnel training,quality con-trol,quality supervision,process control and biological safety,the elements of laboratory quality management were analyzed,and effectively measures were put forward to improve the management level. RESULTS & CONCLUSIONS:The laboratory should es-tablish strict,standardized,systematic laboratory management rules and regulations,quality documents,operating procedures, work guide,etc. to achieve documented management;develop various forms of annual training and assessment programs to con-struct a professional team;strengthen internal(developing an annual internal quality monitoring plan,conducting quality control ac-tivities,regularly checking the suitability of the medium) and external (capability testing,inter-laboratory comparison) manage-ment control level,and reasonably formulate the contents and frequency by combing with actual situation. Besides,the laboratory should strengthen inspection process control,attach great importance to the biological safety management to reduce the risk of labo-ratory quality to a minimum.

Objective To analyze the dermatology journals indexed by SCI‐E and papers published in recent 5 years ,to pro‐vide advices on periodical selection and contribution .Methods We mainly adopted the bibliometrics methods and word frequency a‐nalysis method to analyze 10 targets in 62 SCI‐E dermatology journals .Results Main publishing nationality were United States , England ,Denmark and Germany ,publishing language was English ,main publishing cycle period was bimonthly journals ,the highest impact factor was 6 .372 .The theme of published content was relatively concentrated ,reviewers′duration was fast and reasonable , 80 .7% submission of the journal was easy .Main type of literature published in recent 5 years is article ,review ,editorial and letter . 58 SCI‐E dermatology journals accept articles from China .Conclusion We suggest that the authors should firstly check the name of dermatology and cross‐disciplinary journals indexed by SCI‐E ,and evaluate the theme of published content ,rate of publishing article contributed from China ,difficulty degree for contribution ,reviewers′duration ,publishing cycle and impact factor when contribution articles .

Objective To investigate the correlation between the distribution of the charactcristics of TCM syndrome of alcoholic liver fibrosis and alcoholic consumption. Methods Our group studied clinical cases in multi-center prospective method. In virtue of Factor Analysis, we explored the characteristics of TCM syndrome of alcoholic liver fibrosis, and the forming characteristics of alcoholic liver fibrosis in inland. Rerults Among the subjects, the characteristics of TCM syndrome of alcoholic liver fibrosis mainly manifested excess syndrome with the common manifestations of deficiency and excess. The visceral position was mainly in liver and spleen, related to gallbladder and stomach. The result suggested that stagnation of Qi stagnation, blood stasis, damp heat, with deficiency of genuine Qi were the interpretation of the causes of alcoholic liver fibrosis. The total alcoholic consumption was one of the important factors which effected on the distribution of thecharacteristics of TCM syndrome of alcoholic liver fibrosis. Total bilirubin had mostly striking effect on the distribution of the characteristics of TCM syndrome. Conclusions The total alcoholic consumption was one of the important factors which effected on the distribution of the characteristics of TCM syndrome of alcoholic liver fibrosis.

Objective To investigate the association between third-trimester gestational weight gain rate (GWGR) and both maternal and neonatal health outcomes in a normal glucose tolerance obstetric population.Methods This was a retrospective cohort study of full-term singleton live births (n=1 967) in women with a normal oral glucose tolerance test (OGTT) tested at 24-28 gestational weeks,who gave birth at Beijing Obstetrics and Gynecology Hospital,Capital Medical University,between January and December in 2013.The subjects were divided into three groups based on third-trimester GWGR category of the 2009 Institute of Medicine (IOM) guidelines.Each group was divided into three subgroups by pre-pregnancy body mass index (BMI):low (＜18.5),normal (≥ 18.5-＜25.0),and high (≥ 25.0).One-way analysis of variance,Chi-square or Fisher's exact test,Logistic regression and corrected analysis were performed for statistical analysis.Results (1) Of the 1 967 women analyzed,third-trimester weight gain distribution was normal in 575(29.2％),excessive in 982(49.9％),and insufficient in 410(20.8％).No significant differences were found in terms of age,parity,education level,family history of diabetes or hypertension among the GWGR groups (all P＞0.05).(2) The mean third-trimester weight gain in the 1 967 normal OGTT women was (0.56±0.23) kg/week and the mean neonatal birth weight was (3 442±396) g.The above two parameters were linearly correlated (Y=103.839X+3383.752,r=0.621,P＜0.01).The excessive GWGR group had higher birth weight infants than the normal GWGR group [(3 463.1±417.3) vs (3 427.4±376.1) g,F=4.901,P=0.014].Women in the insufficient GWGR group had lower birth weight infants (3 375.1 ±370.1) g than those in the normal GWGR group (F=4.408,P=0.021).Compared to the normal GWGR group,the excessive GWGR group was associated with an increased risk of fetal macrosomia (OR=1.59,95％CI:1.10-2.30) and low birth weight infants (OR=2.25,95％CI:1.03-4.94),and decreased odds of normal birth weight deliveries (OR=0.81,95％CI:0.77-0.95).The insufficient GWGR group was associated with an elevated risk of low birth weight infants (OR=3.21,95％CI:2.56-7.51,P＜0.01),but not related to the risk of fetal macrosomia on normal birth weight deliveries (all P＞0.05).(3) Compared to the normal GWGR group,the excessive GWGR group had an increased incidence of cesarean section [30.2％ (297/982)vs 22.2％ (128/575)] and hypertensive disorders of pregnancy [4.0％ (39/982) vs 1.9％ (11/575)] (all P＜0.01).No significant differences in the risk of surgical delivery and pregnancy-related hypertension were observed in the insufficient GWGR group compared to the normal GWGR group.No significant differences in the odds of neonatal intensive care unit admission were noted among the three GWGR groups (P＞0.05).(4) In the normal and insufficient GWGR groups,no differences in neonatal birth weight or risk of small-for-gestational age (SGA)or large-for-gestational age (LGA) were seen in any of the BMI subgroups (all P＞0.05).In the excessive GWGR group,the high pre-pregnant BMI subgroup showed higher neonatal birth weight than the normal pre-pregnant BMI subgroup [(3 552.3±445.0) vs (3 481.8±416.1) g,P＜0.01],and the low pre-pregnant BMI subgroup showed lower neonatal birth weight (3 352.7 ± 371.2) g than the normal pre-pregnant BMI subgroup (P＜0.01).Moreover,the high pre-pregnant BMI subgroup in the excessive GWGR group had an increased risk of fetal macrosomia (OR=1.60,95％CI:1.11-2.81).Conversely,the low pre-pregnant BMI subgroup in the excessive GWGR group had a decreased risk of fetal macrosomia (OR=0.52,95％CI:0.29-0.97) (all P＜0.05).The high BMI subgroup had a greater risk of hypertensive disorders of pregnancy than the normal BMI subgroup in all GWGR groups (allP＜0.05).The incidence of surgical delivery or NICU admission was not significantly different among the three GWGR subgroups.Conclusions Excessive weight gain in third-trimester is common in normal OGTT women.Excessive gestational weight gain is associated with adverse maternal and neonatal outcomes.Thus,gestational weight gain in the third-trimester should be adequately monitored and a balance in weight gain within the range recommended by the 2009 IOM guidelines should be established in normal OGTT pregnant women.

Objective To establish an HPLC method for the simultaneous determination of hydroxysafflor yellow A, rutin, quercetin, and kaempferol in Carthamus tinctorius L..Methods The Agilent ZORBAX SB-C18 column (4.6 mm × 250 mm, 5 μm) was used with diode array detection. The mobile phase was 0.5% phosphoric acid (A) and methanol (B) to separate the four components by gradient elution at 30℃; the flow rate was 0.8 mL/min; the injection Volume was 10 μL; the detection wavelength was at 360 nm.Results The linear ranges of hydroxysafflor yellow A, rutin, quercetin, and kaempferol were 0.0776–0.7760 μg (r=0.9999), 0.0460–0.4600 μg (r=0.9996), 0.0064–0.0640 μg (r=0.9999) and 0.0128–0.1280 μg (r=0.9997), respectively. The average recoveries of four components were 99.68% with RSD=2.29% (n=6), 99.78% with RSD=1.52% (n=6), 102.03% with RSD=1.02% (n=6), 97.03% with RSD=2.05% (n=6), respectively.Conclusion The method is convenient, accurate, sensitive, stable and repeatable, which can be a method for quality control of Carthamus tinctorius L..

To investigate the role of AQP9 in brain edema, the expression of AQP9 in an infectious rat brain edema model induced by the injection of lipopolysaccharide (LPS) was examined. Immunohistochemistry and reverse transcription-polymerase chain reaction (RT-PCR) analysis demonstrated that the expressions of AQP9 mRNA and protein at all observed intervals were significantly increased in LPS-treated animals in comparison with the control animals. Time-course analysis showed that the first signs of blood-brain barrier disruption and the increase of brain water content in LPS-treated animals were evident 6 h after LPS injection, with maximum value appearing at 12 h, which coincided with the expression profiles of AQP9 mRNA and protein in LPS-treated animals. The further correlation analysis revealed strong positive correlations among the brain water content, the disruption of the blood-brain barrier and the enhanced expressions of AQP9 mRNA and protein in LPS-treated animals. These results suggested that the regulation of AQP9 expression may play important roles in water movement and in brain metabolic homeostasis associated with the pathophysiology of brain edema induced by LPS injection.
Aquaporins/genetics ; Aquaporins/*metabolism ; Blood-Brain Barrier/metabolism ; Brain/drug effects ; Brain/physiology ; Brain Edema/chemically induced ; Brain Edema/*metabolism ; Lipopolysaccharides ; Rats, Sprague-Dawley ; Water/physiology

Objective To provide evidence for diagnosis and treatment by investigating the pathogens and drug resistance of immunocompromised host (ICH)pneumonias.Methods Statistical method was used to analyze retrospectively the data of pathogens and drug resistance of ICH pneumonia treated in our hospital in 2014. Results We confirmed 187 cases of ICH pneumonia by pathogen study of 324 patients.Gram-negative bacillus (68.42%)was the main pathogen of ICH pneumonia.Acinetobacter baumanii (AB)accounted for the first place. Staphylococcus aureus infection was still the first of Gram-positive coccus.Conclusion Gram-negative bacillus was the main pathogen of ICH pneumonia in our hospital and the isolated bacteria show strong antibiotic resistance. Therefore,it is necessary to strengthen dynamic monitoring of pathogens in the secretions from ICH pneumonia patients’ lower respiratory tract and drug resistance.It is suggested that clinicians make anti-infection treatment cover drug-resistant bacteria.