Objective:To review the clinical efficacy of botulinum toxin type A(BTX-A)in the treatment of trigeminal neuralgia (TN).Methods:The main bibliographic databases were searched from English and Chinese literatures on the topic and the refer-ences of the identified articles were also searched for additional studies.2 reviewers assessed the quality of the included studies and extracted data duplicate.Results:3 Chinese and 1 English reports with 203 participants were included.However,substantial hetero-geneity(I2 =77%)precluded meta-analysis and the results were individually described.3 trials reported that BTX-A was more effec-tive than the control and 1 showed no difference between groups.Adverse effects of BTX-A were reported in 4 reports and were mild to moderate.Conclusion:BTX-A is effective in treating TN with few adverse effects.However,the evidence is weak because of a few studies included and most of them with low methodological quality.Further studies with high quality are needed to testify the evidence.

Objective The aim of the study was to investigate the effects of inflammation on the biological be-haviors of the VF -MSCs and provide the theoretic basis for the repair of the vocal folds which were damaged by in-flammation .Methods The inflammatory vocal fold tissues and normal vocal fold tissues were respectively derived from the vocal cord polyp and normal tissues of the hypopharyngeal carcinoma patients .The HE staining ,masson trichrome staining and elastin van gieson (EVG) staining were performed to detect the effects of inflammation on the collagenous fiber and elastic fibers of the vocal fold lamina propria .The cell colony formation analysis and MTT cell growth curve were used to detect the effects of inflammation on the proliferation of VF -MSCs .The effects of in-flammation on the multi-directional differentiation of VF -MSCs were evaluated by inducing the VF -MSCs to dif-ferentiate into osteoblasts and lipoblasts .Results The results of masson trichrome staining and EVG staining showed that in the inflammatory vocal fold lamina propria collagen fibers became thicker and the amount of collagen fibers increased ,while elastic fibers became thinner and the amount of elastic fibers decreased .Compared with the vocal fold mesenchymal stem cell (VF-MSCs) in normal vocal folds ,VF-MSCs in inflammatory vocal folds pro-liferated more significantly ,but the osteogenic differentiation and adipogenic differentiation of VF -MSCs in inflammatory vocal folds were restrained .Conclusion Inflammation enhanced the compressive resistance ,abated the elasticity , and restrained the multi -directional differentiation of VF -MSCs .

Objective This paper aimed to evaluate the effect of preoperative embolization on the intraoperative blood loss of sacrum chordoma.Methods forty patients involved with the spinal chordoma were retrospectively analyzed in this study.The preoperative embolization group consisted of 16 sacrum chordoma patients were embolizationed by gelatin sponge particles (10 male and6 female)age from 35 to 71 years old,(mean 57.9 years) ; and the control group consisted of 4 sacrum chordoma patients (3 male and 1 female),age from 43 to 61 years old(mean 51.8 years).analyzed the predictive value of preoperative embolization in correlation with the intraoperative blood loss.Results The mean blood loss (2025.0 ±818.5)ml of sacrum chordoma in the preoperative embolization group was insignificantly than that (1950.0 ± 802.1) ml in the control group,(P ＞ 0.05).Conclusion The study shows that the preoperative feeding artery embolization of the spinal tumor has no significant effect on the intraoperative blood loss in the surgical excision of the sacrum chordoma.

Objective To know the eutrophic state of Fenhe 1 reservoir. Methods The eutrophic level of Fenhe reservoir 1 was evaluated through measuring transparence,the total concentration of nitrogen(TN),phosphorus(TP),chlorophyll-a level(Chla) and the total count of the algal cells and calculating water TLI(∑). Results Water transparence in low water period was higher than that in common water period,TN concentration in low water period was higher than that in common water period,and it obviously exceeded the related standard limit in Surface Water Environment Quality Standard (GB3838—2002); TP concertration in common water period and low water period did not exceed the limit; Chla level was low;TLI(∑) in common water period and low water period was lower than 50. The total count of the algal cells was 1.67?106/L in low water period,which was much more than that(9.5?104/L) in the common water period. Conclusion Fenhe reservoir 1 is in mesotropher state.

Objective To explore the value of the square for intracranial puncture orientation applying in the intracranial hematoma microinvasive craniopuncture scavenging technique. Methods Fifty-one patients with hypertensive cerebral hemorrhage were randomly enrolled into the orientation square group( n=27)and the CT orientation routine group (n=24). The puncture point was fixed by certain wayin each group. Both groups received the same intracranial hematoma microinvasive craniopuncture scavenging technique. The punctural precision,the time spended for puncture orientation and the curative effect in two group were observed and compared. Results The deflected rate(18.5%)of the orientation square group was lower than that routine group significantly(50.0%)(P

Objective To identify the outcomes and effect of applying the health check-up nursing quality control system developed as supervised by JCI quality assessment standards.Methods The monitoring system for nursing quality in the health check-up department is established within the framework of the hospital quality improvement committee and in line with characteristics of the department.Quality improvement tools may be called into play for analysis and decision making to revolve critical problems found in health check-up nursing,upgrading nursing quality and customer satisfaction.Results Significant rise of health check-up quality and customer satisfaction for nurses,and the nurses are trained in quality control knowledge and get further involved in quality management of their department.Conclusion Health check-up nursing management system under the JCI standard is conducive to raising the nursing quality,and helps nurses with problem analysis and solution.

In recent years,many new microtubule-associated proteins are found or studied extensively in the treatment of breast cancer.End-binding protein 1 (EB1)has the promotional effects on cell proliferation and enhances the carcinogenesis of breast cancer,and also can affect the chemosensitivity of the treatment. MAP7 domain-containing protein 3 (Mdp3)expresses in breast epithelial cells and plays an important role on the growth and metastasis.Nucleolar and spindle-associated protein (NuSAP)gene is up-regulated in breast cancer and is considered as a biomarker for breast cancer.Tau expression level has a strong correlation with estrogen receptor (ER),progesterone receptor (PR)and human epidermal growth factor receptor-2 (HER-2), and it remains controversial in the results of the effect of paclitaxel chemotherapy and hormone treatment.

Objective To compare postoperative complications between endoscopic thyroidectomy via breast areola approach and open thyroidectomy.Methods From June 2011 to June 2014,475 patients underwent thyroidectomy.Patients were divided into traditional open group (251 cases) and endoscopic thyroidcctomy group (224 cases).Postoperative complications were recorded and compared.Results All operations were successfully completed.There was no case in endoscopic thyroidectomy group converted to traditional open surgery.There was no serious postoperative complication occurred,including injury to trachea,mediastinal emphysema,intraoperative massive hemorrhage and cardiopulmonary accidents.The difference of postoperative complication between two groups had no statistical significance (all P ＞0.05) as to permanent recurrent laryngeal nerve injury,Homer syndrome,permanent hypoparathyroidism,chyle leakage,postoperative hemorrhage and infection of incision.However,postoperative ecchymosis was more often in endo group (P ＜0.05).Conclusions Compared to traditional open thyroidectomy,endoscopic thyroidectomy via breast areola approach has significant cosmetic advantages without increasing the incidence of major postoperative complications.

Objective To evaluate the effect of immune fluorescence chromatography on glycosylated hemoglobin (HbA1c). Methods The precision of immune fluorescence chromatography was evaluated with samples of 6.0% and 8.0% fixed value. Group of High performance liquid chromatography (HPLC) as control, HbA1c for 200 samples of EDTA-K2 anti-coagulated whole blood were detected by immune fluorescence chromatography to synchronous blinded trial. Results As to the precise of immune fluorescence chromatography in the samples of 6% and 8%, values of coefficient of variation were 5.1% and 5.3%, respectively. The linear regression equation of immune fluorescence chromatography and HPLC was Y=-0.110+1.021X and the correlation coefficient was 0.982. 6.0% and 8.0% as the cut-off value, kappa values were 0.950 (P < 0.001) and 0.922 (P < 0.001), respectively. Conclusion Immune fluorescence chromatography and HPLC is consistent with detection of HbA1c, which can be used for clinical detection of HbA1c.

AIM: To study the effects of disulfiram/copper complex (DSF/Cu) on ultrastructures and mechan-ical properties of human breast cancer and normal breast epithelial cells by atomic force microscopy (AFM) based on the nanoscale resolution and piconewton force measurement level.METHODS: The change of cell cycle and apoptotic rate of MCF-7 cells and MCF-10A cells induced by DSF/Cu were compared by flow cytometry.The cell surface morphology, ultra-structure, height, width and roughness were detected by AFM.The effects of DSF/Cu on the hardness (Young’s modu-lus) of the 2 kinds of cells were determined by AFM with indentation technique.RESULTS: DSF/Cu significantly in-duced apoptosis of the MCF-7 cells in a dose-dependent manner, whereas had little effect on the MCF-10A cells.The cell cycle analysis showed that DSF/Cu induced G2 /M arrest in the MCF-7 cells, but led to G0 /G1 arrest in the MCF-10A cells.The AFM images showed that the MCF-7 cells shrank and showed smaller and smoother morphology, and the filopo-dia were retracted obviously, even some became into lamellipodia, or disappeared completely after treated with DSF/Cu at concentrations of 400 and 800 nmol/L.The quantitative analysis indicated that the MCF-7 cells showed smaller width and
larger height, and the root mean square roughness and average roughness were decreased significantly in a dose-dependent manner after treated with DSF/Cu at concentrations of 400 and 800 nmol/L.However, little effect in the MCF-10A cells was observed.The biomechanics test at a single cell level demonstrated that the Young’s modulus of the MCF-7 cells and MCF-10A cells were both increased, yet the proportion increased in the MCF-7 cells was much higher than that in the MCF-10A cells after treated with DSF/Cu at concentrations of 400 and 800 nmol/L.CONCLUSION: DSF/Cu has strong antitumor effect on breast cancer with high efficiency and low toxicity by changing the properties of the biomechanics specifically.