Objective:To observe the clinical efficacy of Xihuang Pills combining with chemotherapy in treating breast cancer and the effects on CD+4,CD+8.Methods:Selecting 60 patients with breast cancer,the control group of 30 was treated with chemotherapy,the treated group of 30 was treated with Xihuang Pills combining with chemotherapy for two chemotherapy cycles,before and after treatment the CD+4,CD+8 levels were detected.Results:The traditional Chinese medicine syndrome and immune function in treated group was superior to those in control group,the difference between those two groups was statistically significant(P

Esophageal cancer is one of the most high incidence of malignant tumors in China. However, the evaluation of chemoradiotherapy curative effect for esophageal cancer is lack of accurate and uniform criteria. In recent years, the imaging evaluation methods for chemoradiotherapy response in esophageal cancer have made some progress. The methods mainly include X-ray barium examination, endoscopic ultrasonography (EUS), computed tomography (CT), magnetic resonance imaging (MRI), positron emission tomography-computed tomography (PET-CT), and so on. Imaging examination has the advantages of safety, non-invasion and repeatability, and so on, which is a progressing tool for curative effect evaluation. Current status of the application of medical imaging which is used to evaluate esophageal cancer chemoradiotherapy curative effect were reviewed in this paper.

?Objective: To study the effects of endogenous bone morphogenic protein 2(BMP 2) and 6(BMP 6) in rat facial nerve after injury. Methods: Facial nerve trunk was cut and then anastomosed in 30 adult male Sprague Dawley rats,another 6 rats without operation were used as the controls. Every 6 rats were killed 6 ,72 h,1, 2 and 4 weeks respectively after operation and nerve specimens were immunohistochemically examined for BMP 2 and BMP 6 expression. Result:In the control nerve the gray level of BMP 2 and BMP 6 were 210.89?8.21 and 232.03?9.25 respectively. 6, 72 h,1, 2 and 4 weeks after operation the gray level of BMP 2 were 242.83?11.01,240.67?7.91,234.46?5.28,232.12?7.27 and 220.71 ?10.19;that of BMP 6 210.75?5.19,204.08?10.85,198,91?8.58,186.37?4.11 and 184.62? 8.45 ,respectively. Conclusion: BMP 2 may play a role in the early irritable actions when facial nerve was injured. However, BMP 6 might have functions in the regeneration of facial nerve during later period after injury.

Objective:To investigate the characters of ERP in undergraduates with different trait anxiety during exam stress. Methods: By the State Trait Anxiety Inventory, one group of 15 undergraduates with high trait anxiety and another group with low trait anxiety were chosen from 200 participants in Band 4 College English Test. Their change of P3 were observed through NeuroScan 32 Channel ERP System during pre- exam and post- exam. Results: The amplitude of P3 of the group with low trait anxiety was enhanced while another group with high trait anxiety had no significant difference after exam; The latency of P3 of the group with low trait anxiety were decreased while another group with high trait anxiety had no significant difference after exam; There weren’t significant differences in amplitude and latency of P3 between high trait anxiety group and low trait anxiety group before exam; There were significant enhance of amplitude of P3 and decrease of latency of P3 in low trait anxiety group after exam as compared with high trait anxiety group. Conclusion: The result suggests different trait anxiety groups have different cognitive functions. When confronting with the same stress.

Objective To formulate paclitaxel loaded polymer nanoparticle and evaluate it's application in treatment of bladder.Methods Paclitaxel loaded Poly (lactide-co-glycolide) (PLGA) nanoparticles were formulated with microemulsion method,Polyvinyl alcohol(PVA) was used as surfactant.Transferrin (Tf) was used to modify the nanoparticles.The size,Z-potential,drug loading,drug release,cytotoxicity of bland nanoparticles and paclitaxel-loaded nanoparticles on bladder cancer cell line J-82 were measured.Results The size of nanoparticles was about 200 nm,Z-potential was-24 mV,drug loading was about 6.5％ (w/w),cumulative drug release showed two phase curve.The size of Tf modified nanoparticles was a little bigger than no modified nanoparticles.The Z-potential,drug loading,drug release was similar.Two kinds of blank nanoparticles showed no cytotoxicity on bladder cancer cell line J-82.However,both paclitaxel-loaded nanoparticles had significantly higher cytotoxicity on J-82 compared to paclitaxel solution.Conclusions PLGA nanoparticle is a promising drug delivery vehicle,which could significantly improve the anticancer effect of paclitaxel on bladder cancer.

To investigate the role of AQP9 in brain edema, the expression of AQP9 in an infectious rat brain edema model induced by the injection of lipopolysaccharide (LPS) was examined. Immunohistochemistry and reverse transcription-polymerase chain reaction (RT-PCR) analysis demonstrated that the expressions of AQP9 mRNA and protein at all observed intervals were significantly increased in LPS-treated animals in comparison with the control animals. Time-course analysis showed that the first signs of blood-brain barrier disruption and the increase of brain water content in LPS-treated animals were evident 6 h after LPS injection, with maximum value appearing at 12 h, which coincided with the expression profiles of AQP9 mRNA and protein in LPS-treated animals. The further correlation analysis revealed strong positive correlations among the brain water content, the disruption of the blood-brain barrier and the enhanced expressions of AQP9 mRNA and protein in LPS-treated animals. These results suggested that the regulation of AQP9 expression may play important roles in water movement and in brain metabolic homeostasis associated with the pathophysiology of brain edema induced by LPS injection.
Aquaporins/genetics ; Aquaporins/*metabolism ; Blood-Brain Barrier/metabolism ; Brain/drug effects ; Brain/physiology ; Brain Edema/chemically induced ; Brain Edema/*metabolism ; Lipopolysaccharides ; Rats, Sprague-Dawley ; Water/physiology

BACKGROUND: The degree of neurofilament (NF) phosphorylation is closely correlated with the occurrence of Alzheimer disease (AD), and apolipoprotein E4 (apoE4) is a generally acknowledged liability factor for AD, but the effect and mechanism of apoE4 on the NF phosphorylation in neurons are not very clear. It has been reported that in the neurons cultured in vitro and in brain tissue of AD patients, the amino acid residues of apoE4 protein C terminal (272-299) could be truncated by hydrolysis,and produce truncated-apoE4 fragment. The latter interacts with the NF phosphorylation in neurofibrillary tangles (NFTs), which are the characteristic pathological changes of AD.OBJECTIVE: To observe the effect of truncated-apoE4 overexpression on the NF phosphorylation in the cultured neurons.DESIGN: A non-randomized controlled observation.SETTING: Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Tongji Medical College, Huazhong University of Science and Technology.MATERIALS: The experiment was carried out in the Laboratory of Biochemistry and Molecular Biology, Tongji Medical College, Huazhong University of Science and Technology in December 2005. The mice neuroma cell strain N2a was provided by Dr. Xu.METHODS: The truncated-apoE4(△272-299) cDNA was subcloned into pEGFP-c3 to form pEGFP-T-apoE4 recombinant. Then pEGFP-c3, pEGFP-apoE4 and pEGFP-T-apoE4 were transfected into N2a cells by lipofectamine2000 respectively. NF phosphorylation was detected by Western blot assay. The activities of glycogen synthase kinase-3 (GSK-3) and cyclin-dependent kinase 5(CDK-5) were measured.MAIN OUTCOME MEASURES: The degree of NF phosphorylation and the activities of GSK-3 and CDK-5 were mainly observed.RESULTS: In the transfected groups, the contents of phosphorylated NF were significantly increased, the GSK-3 activities were significantly increased, which were the most significant in the pEGFP-T-apoE4 transfected group (P＜0.05), but the CDK-5 activities were not significantly different from that in th e control group (P＞0.05).CONCLUSION: These results indicate that in vitro overexpression of truncated-apoE4(△272-299) can lead to NF hyperphosphorylation by activating of GSK-3 but not CDK-5, which may be the underline mechanism of AD induced by truncated-apoE4(△272-299).

Objective To explore the expression of small GTP-binding protein TC10 during nerve injury and construct eukaryotic expression vector carrying rat TC10 cDNA. Methods Total RNA was extracted from rat facial nucleus, and the change of rat TC10 full-length cDNA was investigated by RT-PCR. PCR product was inserted into eukaryotic expression vector pcDNA3 to construct the pcDNA 3-TC10. The plasmid was identified by restriction enzyme analysis and sequencing analysis. Results After injury, the expression of TC10 rapidly increased, Restriction enzyme assay and sequencing assay showed that the construct we got was rat TC10 full-length cDNA construct.

Objective:To examine the influence of exam stress on the sustained attention of students with different levels of trait anxiety. Methods: With the State-Trait Anxiety Inventory (STAI), 10 students with high trait anxiety (high trait anxiety group, H group) and 10 with non-high trait anxiety (non-high trait anxiety group, NH group) were randomly sampled from 400 students taking the coming College English Test-Band 4 (CET-4). Their attention was measured with digit cancellation test and the event related potentials during the control phase (the period was chosen 2 weeks after the beginning of the semester and there was no examination in the following 3 months) and the stress phase ( from 2 weeks to 1 day before CET-4 examination). Results: The right cancellation number of the NH group was higher than that of the H group(F=8.178, P=0.007). During the control phase the P300 amplitude and latency were similar between the 2 groups (F= 0.125, 0.127, respectively;P= 0.728, 0.726, respectively). During stress phase the P300 amplitude of the H group was significantly lower than that of the NH group(F=12.913, P=0.002)and the P300 latency of the H groups was longer than that of the NH group(F=16.952, P= 0.001). In NH group, the P300 latency in the stress phase was significantly shorter than that of the control phase (F= 6.514, P=0.020). Conclusion: Exam stress has different influences on the sustained attention of people with different levels of trait anxiety, which suggests that people with high trait anxiety are the target population for stress management.