OBJECTIVE: To establish HPLC fingerprint of flavonoids from Bauhinia blakeana and to provide reference for harvest, application and quality evaluation of B. blakeana. METHODS: RP-HPLC method was used to determine the content of flavonoids from 10 batches of B. blakeana from different habitats and in different harvest periods with rutin and quercetin as control. The similarity was calculated using TCM fingerprint similarity evaluation system. The separation was performed on Hypersil ODS C18(250 mm?4.6 mm,5 ?m) column with mobile phase consisted of methanol-0.4% phosphoric acid solution (gradient elution) at flow rate of 0.7 mL?min-1 lasting for 40 min. The UV detection wavelength was set at 360 nm and column temperature was at 25℃. RESULTS: The results showed that 14 peaks were common. The similarity index of 10 batches B. blakeana samples were between 0.88 and 0.94. The results indicated chemical components were similar but content of the compounds was different. CONCLUSION: Established HPLC fingerprint of B. blakeana is stable, reliable and reproducible, which is valuable for harvest, application and quality evaluation of B. blakeana.

Objective To research the consonant pronunciation of patients with spastic dysarthria as a result of cerebral palsy and supply a theoretical basis for their rehabilitation.Methods Twenty-eight cerebral palsy patients with spastic dysarthria were examined using a dysarthria examination scale developed by the China Rehabilitation Research Center.The whole process of the tests was recorded using Cool Edit recording software.Praat4 was used to evaluate their pronunciation according to the pronunciation criteria of “experimental phonology”.Their error rates on 21 consonants were counted and the characteristics of the articulation errors were summarized.Results According to the location of articulation,the inaccuracy rates on labials,dentals,apicals,blade-palatals,palatals and velars in the 28 patients were 21％,71％,53％,71％,54％ and 36％ respectively.Conclusions Patients with spastic dysarthria as a result of cerebral palsy have the most difficulty pronouncing dentals and blade-palatal consonants.They find labial consonants the easiest.

Recent years have witnessed great development of TLA (Total Laboratory Automation) technology, however, its application hit the bottleneck of high cost and openess to other parties' instruments. Specifically speaking, the initial purchase of the medical devices requires large sum of money and the new system can hardly be compatible with existing equipment. This thesis proposes a new thought for system implementation that through incremental upgrade, the initial capital investment can be reduced and through open architecture and interfaces, the seamless connection of different devices can be achieved. This thesis elaborates on the standards that open architecture design should follow in aspect of mechanics, electro-communication and information interaction and the key technology points in system implementation.
Automation, Laboratory ; Computer Systems

Objective This report investigate the intervention effect of the water extract of Alpinia oxyphylla Miq. fruit (AOF)on memory impairment and the mechanism in cerebral ischemia rats. Methods 48 Rats were randomly divided into sham-operated group(n=12), ischemia group(n=12), AOF group Ⅰ( n= 12)and AOF group Ⅱ (n= 12). The model of transient cerebral ischemic/reperfusion was made by bilateral occlusion of common carotid arteries in rats and combination with reducing blood pressure with an abdominal injection of so-dium nitroprusside. Learning-memory ability was observed by step through test. The content of NO and the activi-ties of NOS were measured in hippocampus. Results In step through test,the latency in ischemia group[(143.8±65.2)s]significantly decreased, the number of errors (8.9±4.2 ) significantly increased compared with sham-operated group [latency: (257.2±67.1 ) s; number of errors: (1.7±1.1 ), P<0.01 ]. The latency in AOF group Ⅰ and AOF group Ⅱ[(186.5±46.2) s, (193.4±43.7 ) s ] significantly increased, the number of errors (6.1±2.9,5.2±2.1 ) significantly decreased compared with ischemia group(P<0.05, P<0.01). In hippocampus, the content of NO and the activities of NOS in ischemia group [(56.53±27.42) nmol/mg prot, (17.23±5.64) nmol/mg prot] significantly increased compared with sham-operated group[ (40.02±17.9 ) nmol/mg prot, ( 10.46±6.15)nmol/mg prot], and in AOF group Ⅰ and AOF group Ⅱ [content of NO:group Ⅰ (46.60 ±20. 26)nmol/mg prot,group Ⅱ (42.38±21.23) nmol/mg prot ;activities of NOS:group Ⅰ (13.98±5.13 ) nmol/mg pint,group Ⅱ(13.61±5.27) nmol/mg prot] significantly decreased compared with ischemia group(P<0.05). Conclu-sion AOF could significantly ameliorate the memory impairment in cerebral ischemic rats. Its effects may be in-volved in the decrease of content of NO and activities of NOS in hippocampus.

BACKGROUND: Hypogonadism happens gradually with body aging. Traditional Chinese medicine views that deficiency of essential qi is the chief reason for body aging. OBJECTIVE: To observe the influence of tiannianyin, the Chinese herb , on serum testosterone, superoxide dismutase (SOD) activity in testicular tissue and expression of proliferating cell nuclear antigen of spermatogenic cells in testes in subacute senile rats induced by D-galactose. DESIGN: Randomized controlled experiment. SETTING: Basic Medicine Institute of Chengde Medical College. MATERIALS: The experiment was perlormed in Basic Medicine Institute of Chengde Medical College from April to July 2003, in which, 40 adult male SD rats were employed and randomized into 4 groups, 10 rats in each .In normal group, no any management was done. In model group, medication group and negative control, D-galactosie was used for abdominal injection continuously at 200 mg (kg·d), once a day, totally for 40 days to prepare the model of subacute senile animal. In model group, no any management was done after modeling success. In medication group, gastric infusion with tiannianyin. Tiannianyin was administrated everyday after modeling success, once a day, totally for 30 days. In negative control, gastric infusion with physiological saline of same dosage was administrated after modeling success for same days. METHODS: Enzyme linked immunosorbent assay (ELISA) was used for determination of serum testosterone content, xanthine oxidase assay was used for determination of SOD activity in testicular tissue and immunohistochemical method was used to observe expression of proliferating cell nuclear antigen in rats of every group. MAIN OUTCOME MEASURES: ① serum testosterone content; ② SOD activity in testicular tissue; ③ expression of proliferating cell nuclear antigen of spermatogenic cells. RESULTS: Forty rats all entered result analysis. ① Serum testosterone content: That in model group was lower than normal group(P ＜ 0.05), and that in medication group was higher than model group(P ＜ 0.05). ② SOD activity in testicular tissue: That in model group was lower remarkably than normal group(P ＜ 0.01), and that in medication group was higher remarkably than model group(P ＜ 0.01). ③ Expression of proliferating cell nuclear antigen of spermatogenic cells: That in model group was lower than normal group(P ＜ 0.05), and that in medication group was higher remarkably than model group(P ＜ 0.05). CONCLUSION: Tiannianyin delays degeneration of reproductive function and aging of certain degree.

BACKGROUND: In follow of the gradual decrease of senile gonad function in the body, traditional Chinese medicine views that deficiency of essential qi is the chief reason of body senility. OBJECTIVE: To observe the effects of tian nian yin on serum testosterone content, superoxide dismutase activity in testicular tissue and apoptosis of testicular seminiferous cell in subacute senile rats. DESIGN: Randomized controlled experiment. SETTING: Human Anatomy Department of Chengde Medical College. MATERIALS: The experiment was performed in Institute of Basic Medicine of Chengde Medical College from April to July 2003, in which,40 SD male rats were employed and randomized into 4 groups, 10 rats in each group. In model group, medicinal group and the control, D-galactose was injected 200 mg/(kg·d) to prepare subacute senile animal model, once a day, continuously for 40 days. No any management was given in model group after modeling success. In medicinal group, gastric infusion with tian nian yin [composed of zhi heshouwu (Radix Polygoni Mutiflori Preparatea),huainiuxi (Radix Achyranthis Bidentatae), roucongrong (Herba Cistanchis),fuling (Poria), danshen (Radix Salviae Miltiorrhizae) and yinyanghuo (Herba Epimedii), it was decocted till 1.5 g/mL with raw herbs contained] was administrated once a day, totally for 30 days after modeling success. In the control, gastric infusion was administrated with physiological saline of same usage as the medicinal group after modeling success. INTERVENTIONS: In every group, enzyme linked immunosorbent assay (ELISA) was used to determine testosterone content in serum. Xanthine oxidase assay (XOD) was used to determine superoxide dismutase activity in testicular tissue and immunohistochemical method (IHC) was used to observe apoptosis of testicular seminiferous cell. MAIN OUTCOME MEASURES: ① Testosterone content in serum. ②Superoxide dismutase activity in testicular tissue. ③ Apoptosis of testicular seminiferous cell RESULTS: Forty rats all entered result analysis. ① Testosterone content in serum: Testosterone content in model group was lower than normal group [(0.52±0.15) μg/L, (1.26 0.32) μg/L, (t=3.004, P ＜ 0.05)], and that in medicinal group was higher than model group [(1.16±0.32) μg/L,(0.52 0.15) μg/L, (t=2.321, P ＜ 0.05)]. ② Superoxide dismutase activity in testicular tissue: Superoxide dismutase activity in model group was lower remarkably than the normal group [(107.22±9.33) kNU/g, (147.73±11.90) kNU/g,(t=13.339, P ＜ 0.01)], and that in medicinal group was higher remarkably than model group [(141.05±14.57) kNU/g, (107.22±9.33) kNU/g, (t=9.970,P ＜ 0.01)]. ③ Apoptosis of testicular seminiferous cell: Percentage of apoptotic seniniferous tubule and apoptotic rate of positive cell in model group were higher remarkably thannormal group respectively [(53.95 ±2.02)％compared with (34.21±2.10)％, (8.67±0.80)％ compared with (3.86±0.52)％,(x2=7.9, P ＜ 0.01, x2=5.01, P ＜ 0.05)], and those in medicinal group were lower remarkably than model group[(35.33±2.18)％ compared with (53.95±2.02)％, (4.68±0.74)％ compared with (8.67±0.80)％, (x2=7.02, P ＜ 0.01, x2=3.96, P ＜ 0.05)]. CONCLUSION: The apoptosis of testicular seminiferous cell was increased in senile modelgroup. Tian nian yin in gastric infusion decreased apoptotic rate of positive cell. It is explained that tian nian yin eliminates surplus oxygenic free radical, decreases apoptosis and improves seminiferous function in animal model.

Objective To summarize the clinical features of chronic ruptured patellar tendon, and introduce the augmentation technique of repair of chronic ruptured patellar tendon with high strength polyester sutures and postoperative rehabilitation.Methods Six cases of chronic patellar tendon rupture were treated and reinforced with high strength polyester sutures from 2002 to 2007.Patellar tendon ruptures was primary end Ho -end repaired, and reinforced with 4 polyester sutures that were passed through in-traosseous tunnels within the patella and tibial tubercle.The surgery reestablished knee extensor continuity and restored the length of patellar tendon.Augmentation techniques were reliable, and allowed patients to walk and bend the knee, regain adequate quadriceps strength early.Postoperative rehabilitation: on the 1st day after surgery, walking upright and passive flexion of 90° was allowed, and brace protection was unnecessary; 3 weeks later, straight leg raise; 6 weeks later, brisk walking; 12 weeks later, squatting and climbing stairs; 6 months later, sports was allowed, including the jump and kick.Results All patients were followed up from 1 to 5 years.No patient sustained a re-rupture.All patients satisfied with their result.The Lysholm score were 100.Kach knee had restored full range of motion 6 months later, and regained adequate quadriceps strength.Conclusion Augmentation techniques with high strength polyester sutures is reliable and demonstrate good intermediate to long-term results.It can avoid re-operation for removal of the cerclage wire, allow patients to walk and bend the knee, regain adequate quadriceps strength early.

Objective To evaluate the clinical effects of total parenteral nutrition in the treatment of severe neonates.Methods 45 severe neonates were treated with their essential while injected nutritious liquid.At the same time some concemed targets were tested.Results 2 cases died in 45 neonates,server infections and sudden stop of heartthrob and breath being the causes of death,and the others achieyed satisfactory result.The average body mass increased by 15/30g/d during parenteral nutrition.Conclusion Total parenteral nutrition was clinically significant for increasing the body weight of severe neonates,and it was safe,effective and supportive.

Objective To compare the glycemic control of different management approaches in type 1 diabetic mellitus (T1 DM) children,and to evaluate the influence factors associated with glycemic control.Methods This survey included 68 T1DM children from January 2012 to December 2014 in the Affiliated Hospital of Qingdao University of more than 1 year management.Hemoglobin A1c (HbA1c) levels were detected to assess glycemic control.Twenty-three T1DM children who participated in the World Diabetes Foundation(WDF) management program (group A) were compared with 45 T1DM children who were managed by themselves (group B).The clinical data were analyzed to explore the correlations with glycemic control.Results The HbA1c of 68 children was (8.86 ± 2.00)％,and only 21 cases (30.9％) had optimal HbAlc (HbAlc ＜ 7.5％).The level of HbA1 c of group A (7.60 ± 1.57)％ was significantly lower than that of group B[(9.40 ± 1.91) ％] (t =-4.045,P =0.001).Group A had less fluctuation in blood glucose than group B,which the duration more than 3 years.Duration (F =2.277,P =0.004),frequency of self-monitoring blood glucose (F =9.375,P =0.001),diabetic education (F =3.276,P =0.002),and diabetic diet (t =-2.046,P =0.025) were associated with glycemic control.Conclusions The glycemic control of T1 DM children at the Affiliated Hospital of Qingdao University was at the medium level,most of them haven't reached the target levels.The WDF management program could significantly and continuously monitor the glycemic level.Diabetic education and more frequent self-monitoring blood glucose and diabetic diet are associated with better glycemic control.

Objective To study the difference of transverse incision and longitudinal incision for B-T-B graft harvesting in reconstruction of anterior cruciate ligament.Methods Sixty four cases of reconstruction of anterior cruciate ligament from January 2004 to December 2004 were observed.Transverse incisions were used in 32 cases,and longitudinal incision were chosen in the other 32.The operation time,the length of incision,the thickness of scar,the injury of saphenous nerve and the joint function of knee were compared.Results All patients were followed up more than 1 year.The average duration of surgical procedure with longitudinal incision was 11 minutes shorter than that with transverse incision.The scar of transverse incision is smaller and thinner than that of longitudinal incision.The injury of saphenous nerve and knee joint function of knee revealed no notable difference in the 2 groups.Conclusions Transverse incision for B-T-B graft harvesting in reconstruction of anterior cruciate ligament is a better choice.