Aim To observe the expression of cyclooxygenase COX-1 and 2 in brain after spared nerve injury(SNI).Methods Pain thresholds by hot-plate test were determined before,1 h,12 h,1d,3 d,7 d,14 d,30 d and 60 d after SNI.Three methods were used to evaluate the change of brain COX expressionafter SNI,radioimmunoassay for measurement of PGF_(1?)(catalyzed by COX-1)and PGE_2(catalyzed by COX-2),RT-PCR for mRNA and Western blotting for protein.Time points for assay were before SNI,1 h,12 h,1 d,3 d,7 d,14 d,30 d and 60 d after SNI.Results Pain thresholds of mice decreased significantly during the period of 60 d after SNI(all P

Objective To isolate and identify the antitumor bioactive components from the fermentation of the fungus Penicillium auratiogriseum Sp-19 derived from sponge Mycale plumose.Methods The antitumor components were isolated by bioassay-guided fractionation and using solvent extraction,silica gel column chromatography and preparative HPLC.Their structures were established by physicochemical properties and spectral analyses.The cytotoxicities of compounds were evaluated by SRB method.Results and Conclusion Five alkaloid compounds were isolated and identified as fructigenines A(1),deacetyl fructigenines A(2),fructigenines B(3),1,4-benzodiazepine-2,5-diones(4) and cyclopenin(5),respectively.Compound 4 and 5 showed cytotoxicity at the concentration of 3 ?g?mL~(-1)against tsFT210 cell line.

Objective To revise the disclosure expectations scale and to exam its reliability and validity.Methods Based on researching foreign related questionnaire and preparing test,university students from different grades and majors were chosen as subjects to exam the reliability and validity of questionnaire of stigma for seeking professional psychological help.Results The Cronbach's alpha coefficients of the questionnaire were 0.7412 and 0.7423.The retest reliability of the questionnaire were 0.7527 and 0.7662.There was a significantly positive correlation between risk factor and the self-concealment scale(r=0.6554,P<0.01).There was a significantly negative correlation between benefit factor and the self-concealment scale(r=-0.6752,P<0.01).Confirmatory factor analysis verified the 2 dimensional models.Conclusion The revised disclosure expectations scale has good reliability and validity,and Can satisfy the domestic relevant research and applied demand.

ObjectiveTo explore the weaning method of mechanical ventilation in the treatment of acute severe respiratory failure caused by organophosphate poisoning.MethodsThe clinical features and weaning situation of 51 patients with acute severe respiratory failure caused by organophosphate poisoning by the treatment of mechanical ventilation were retrospectively analyzed.ResultsIn 51 cases,45 cases were cured,the survival rate was 88.2％ and 6 cases died,the fatality rate 11.8％.Conclusion Early ventilator to rescue and choose opportunity for weaning could directly impact on the qualitv of life of patients,and improve outcomes,and reduce mortality.

According to Wiener's cybernetics,the author deduces the fundamental principle of negative feedback teaching and builds its model.On the basis of the model,author raises the approach of negative feedback in pathophysiology teaching.

Objective To evaluate the efficacy of bevacizumab combined with chemotherapy in the treatment of advanced colorectal cancer. Methods 100 cases of patients with advanced colorectal cancer from March 2015 to December 2016 in our hospital were selected as the object, and randomly divided into observation group and control group, 50 cases in each group. Group A was given routine chemotherapy, group B was given combined with chemotherapy and bevacizumab treatment, compared the two groups of patients with clinical efficacy. Results The total effective rate was 28.0％, group B was 54.0％, the difference between the two groups was statistically significant (P<0.05). Conclusion Combined chemotherapy and bevacizumab is effective in the treatment of advanced colorectal cancer and is worthy of promotion.

Objective To find out about the effect of the trustee policy on the quality of medical workers' material life so as to further improve the trustee reform. Methods An investigation was made via questionnaires on the annual income, consumption structure and housing area of 320 medical workers in hospitals that had adopted trusteeship. Results After the adoption of trusteeship in 2002, the quality of all the medical workers' material life was improved, but there was a signiticant difference among the doctors, nurses and medico-technicians (P

Objective To investigate potential mechanism of arsenic trioxide (As2O3) and/or adriamycin (ADM) induces apoptosis in hepatocellular carcinoma (HCC) cells. Methods Human hepatocellular (carcinoma) cells, HepG2, were treated with As2O3 combined with/without ADM in various concentrations for various time, and then the expression of survivin was detected by immunocytochemistry and RT-PCR (respectively). Results (1)Before the cells were interfered with drugs, survivin was highly expressed in HepG2 cells. (2)Expression of survivin was reduced in cells treated with various concentrations of As2O3 or ADM , and the reduction was dependent on durg concentration and time, these two factors were interrelated. (3)Expression of survivin was reduced more markedly in cells treated with ADM than in those treated with As2O3 and the reduction was additionally increased in cells treated with a combination of the two compounds. Conclusions (1)Either As2O3 or ADM can downregulate the expression of survivin in HepG2 cells in a concentration-or time-dependent manner; (2)At the same concentration, ADM is more effective than As2O3 on the downregulation of survivin expression in HepG2 cells, but the effect can be enhanced by combination of these two drugs. (3)As2O3 and ADM may induce apoptosis in HepG2 cells through the downregulation of survivin expression and thus exert their anti-cancer effect. Their combination may enhance their anti-tumor effect, so that in clinical use, the dosage of each durg may be reduced.

Objective To explore an effective treatment for perimenopausal dysfunctional uterine bleeding.Methods 136 patients with perimenopausal dysfunctional uterine bleeding were received mifepristone treatment.Hemoglobin,thickness of the endometrium and sex hormones indicators,including progesterone (P),estrogen (E2),the corpus luteum generated hormone(LH),follicle hormone(FSH),prolactin(PRL) and testosterone(T),were detected before and after treatment.Results The treatment effective rate was 89.7％.After taking a course of treatment,89 cases decreased to restore normal menstrual cycle ;33 cases entered menopause after taking a course of treatment.After treatment,hemoglobin level was significantly increased compared with before treatment (t =3.899,P ＜ 0.05),and endometrial thickness significantly reduced (t =5.652,P ＜ 0.05).After treatment,the P,E2,FSH,LH levels were significantly lower than before treatment(t =5.320,4.309,3.872,3.641,all P ＜ 0.05).T,PRL levels had no significant differences.Conclusion The mifepristone treatment for perimenopausal uterine bleeding have good clinical efficacy,less adverse reactions,and worthy of clinical application.

Renal interstitial fibrosis closely correlates with deteriotation of renal function.The experiments on animal models are necessary for studying renal interstitial fibrosis.Researchers should find right animal models according to different objectives.Research advancement of widely-used eight animal models of renal interstitial fibrosis,such as unilateral ureteral obstruction,cyclosporine A nephropathy and chronic aristolochic acid nephropathy,are reviewed in this paper.