Aim To observe the expression of cyclooxygenase COX-1 and 2 in brain after spared nerve injury(SNI).Methods Pain thresholds by hot-plate test were determined before,1 h,12 h,1d,3 d,7 d,14 d,30 d and 60 d after SNI.Three methods were used to evaluate the change of brain COX expressionafter SNI,radioimmunoassay for measurement of PGF_(1?)(catalyzed by COX-1)and PGE_2(catalyzed by COX-2),RT-PCR for mRNA and Western blotting for protein.Time points for assay were before SNI,1 h,12 h,1 d,3 d,7 d,14 d,30 d and 60 d after SNI.Results Pain thresholds of mice decreased significantly during the period of 60 d after SNI(all P

Objective To explore an effective treatment for perimenopausal dysfunctional uterine bleeding.Methods 136 patients with perimenopausal dysfunctional uterine bleeding were received mifepristone treatment.Hemoglobin,thickness of the endometrium and sex hormones indicators,including progesterone (P),estrogen (E2),the corpus luteum generated hormone(LH),follicle hormone(FSH),prolactin(PRL) and testosterone(T),were detected before and after treatment.Results The treatment effective rate was 89.7％.After taking a course of treatment,89 cases decreased to restore normal menstrual cycle ;33 cases entered menopause after taking a course of treatment.After treatment,hemoglobin level was significantly increased compared with before treatment (t =3.899,P ＜ 0.05),and endometrial thickness significantly reduced (t =5.652,P ＜ 0.05).After treatment,the P,E2,FSH,LH levels were significantly lower than before treatment(t =5.320,4.309,3.872,3.641,all P ＜ 0.05).T,PRL levels had no significant differences.Conclusion The mifepristone treatment for perimenopausal uterine bleeding have good clinical efficacy,less adverse reactions,and worthy of clinical application.

Objective To evaluate the efficacy of bevacizumab combined with chemotherapy in the treatment of advanced colorectal cancer. Methods 100 cases of patients with advanced colorectal cancer from March 2015 to December 2016 in our hospital were selected as the object, and randomly divided into observation group and control group, 50 cases in each group. Group A was given routine chemotherapy, group B was given combined with chemotherapy and bevacizumab treatment, compared the two groups of patients with clinical efficacy. Results The total effective rate was 28.0％, group B was 54.0％, the difference between the two groups was statistically significant (P<0.05). Conclusion Combined chemotherapy and bevacizumab is effective in the treatment of advanced colorectal cancer and is worthy of promotion.

Objective To isolate and identify the antitumor bioactive components from the fermentation of the fungus Penicillium auratiogriseum Sp-19 derived from sponge Mycale plumose.Methods The antitumor components were isolated by bioassay-guided fractionation and using solvent extraction,silica gel column chromatography and preparative HPLC.Their structures were established by physicochemical properties and spectral analyses.The cytotoxicities of compounds were evaluated by SRB method.Results and Conclusion Five alkaloid compounds were isolated and identified as fructigenines A(1),deacetyl fructigenines A(2),fructigenines B(3),1,4-benzodiazepine-2,5-diones(4) and cyclopenin(5),respectively.Compound 4 and 5 showed cytotoxicity at the concentration of 3 ?g?mL~(-1)against tsFT210 cell line.

Objective:To investigate the antitumor effect of Low-Frequency Pulse Forcusd Ultrasound(LFPFU) exposure in human cell line COC1,and to clarify its probable mechanism.Methods:The growth inhibitory effect of ultrasound exposure[0.8MHz,83.6W/cm 2,pulse duration(PD) 50?s;sonication time of 10s] on COC1 cell was observed by MTT colormetric assay.Apoptosis was analyzed using flow cytometry(FCM) and terminal deoxynucleotidyl transferase-mediated dUTP-digoxin nick end labeling(TUNEL),and fluorescent microscope and electron microscope were applied to detect the morphological changes of COC1 cell.The protein expression of bcl-2 and bax affected by LFPFU were determined by FCM and immunofluorescent techniques.Results:Sonication could obviously suppress proliferation of COC1 cells surviving ultrasound exposure.The absorbance value(A 570 )of MTT decreased significantly at 12h,24h,36h and 48h after sonication as compared with control group.The hightest apoptosis rate was found to reach 23.57?4.62% by FCM,and brown-colored positive apoptotic cells were observed with TUNEL method at 6h after sonication.Typical morphological changes of apoptosis such as apoptotic body was found under fluorescent microscope and transmission electron microscope.Fluorescence index(FI) of bcl-2 decreased,and Fl of bax increased after LFPFU exposure.Conclusion:LFPFU can induce apoptosis in vitro in human ovarian cell line COC1 surviving ultrasound exposure.The bcl-2 and bax protein may be involved in the apoptotic process.

Objective:To observe the effects of hydroxychloroquine on insulin sensitivity in high-fat feeding rats.Methods:After administrating hydroxychloroquine,the glucose infusion rate (GIR) determined by euglycemic clamp was used to measure the insulin sensitivity,and determining fasting plasma glucose,lipid and insulin.Results:(1)In high-fat feeding rats,the GIR60-120 was lower and the fasting plasma glucose,insulin,lipid levels were higher than those in normal feeding rats.(2)Hydroxychloroquine,rosiglitazone and metformin increased insulin sensitivity (the value of GIR60-120 was 9.3?1.2,11.6?1.4 and 9.8?1.3 mg.kg/min,respectively) in insulin resistance (IR) models.Moreover,the fasting glucose and some plasma lipid indices were improved in the IR rats with hydroxychloroquine administration.Conclusion:It is indicated that hydroxychloroquine could raise insulin sensitivity of IR rats with high-fat feeding,which was similar to metfomin but lower than rosiglitazone.In addition,hydroxychloroquine also could improve the glucose and blood lipid of the IR rats in some extent.

0.05).Conclusion:HepG2/Adm/nude xenograft established by HepG2/Adm cells transplantation retains the characteristics of MDR phenotype.It can provide an ideal animal model for the study of human tumor MDR.

Objective:To establish a hyperinsulinemic euglycemic clamp technique for the study of insulin sensitivity in Chinese.Methods:A hyperinsulinemic euglycemic clamp technique was used to the study of methodology in 8 normal weight subjects with normal glucose tolerance.Results:(1)When a higher level of insulin was created during maintaining fasting euglycemia,the secretion of endogenous insulin and hepatic glucose production were completely inhibited.The secretion of counter-regulatory hormones(cortisol,growth hormone and glucagon) was not significantly stimulated..(2)During the steady-state of hyperinsulinemic euglycemic clamp,the glucose disposal rate was 9.25?0.25mg/(kg.min)Conclusion:The hyperinsulinemic euglycemic clamp technique was established successfully in Chinese.During the steady-state of exogenous insulin and glucose metabolism,glucose utilization increased.

Objective To investigate potential mechanism of arsenic trioxide (As2O3) and/or adriamycin (ADM) induces apoptosis in hepatocellular carcinoma (HCC) cells. Methods Human hepatocellular (carcinoma) cells, HepG2, were treated with As2O3 combined with/without ADM in various concentrations for various time, and then the expression of survivin was detected by immunocytochemistry and RT-PCR (respectively). Results (1)Before the cells were interfered with drugs, survivin was highly expressed in HepG2 cells. (2)Expression of survivin was reduced in cells treated with various concentrations of As2O3 or ADM , and the reduction was dependent on durg concentration and time, these two factors were interrelated. (3)Expression of survivin was reduced more markedly in cells treated with ADM than in those treated with As2O3 and the reduction was additionally increased in cells treated with a combination of the two compounds. Conclusions (1)Either As2O3 or ADM can downregulate the expression of survivin in HepG2 cells in a concentration-or time-dependent manner; (2)At the same concentration, ADM is more effective than As2O3 on the downregulation of survivin expression in HepG2 cells, but the effect can be enhanced by combination of these two drugs. (3)As2O3 and ADM may induce apoptosis in HepG2 cells through the downregulation of survivin expression and thus exert their anti-cancer effect. Their combination may enhance their anti-tumor effect, so that in clinical use, the dosage of each durg may be reduced.

Objective To revise the disclosure expectations scale and to exam its reliability and validity.Methods Based on researching foreign related questionnaire and preparing test,university students from different grades and majors were chosen as subjects to exam the reliability and validity of questionnaire of stigma for seeking professional psychological help.Results The Cronbach's alpha coefficients of the questionnaire were 0.7412 and 0.7423.The retest reliability of the questionnaire were 0.7527 and 0.7662.There was a significantly positive correlation between risk factor and the self-concealment scale(r=0.6554,P<0.01).There was a significantly negative correlation between benefit factor and the self-concealment scale(r=-0.6752,P<0.01).Confirmatory factor analysis verified the 2 dimensional models.Conclusion The revised disclosure expectations scale has good reliability and validity,and Can satisfy the domestic relevant research and applied demand.