Objective:To investigate the anti-intestinal effect and mechanism of Wnt/β-catenin signaling pathway in Wuzhishan CallicarpanudifloraHook.etArn in Hainan Province.Methods:The colony formation assay was used to detect the effect of Wuzhishan CallicarpanudifloraHook.etArn on the proliferation of intestinal cancer cells in Hainan Province. The effect of Wuzhishan CallicarpanudifloraHook. etArn on the colony growth of intestinal cancer cells was detected by HE staining. The effect of Wuzhishan CallicarpanudifloraHook.etArn on transmembrane protein of intestinal cancer cell line IFITM1 was detected by laser confocal microscopy. Immunocytochemistry was used to detect the effect of Wuzhishan CallicarpanudifloraHook.etArn on the tumor suppressor protein of intestinal cancer cells. Western Blot was used to detect the expression of Wnt/β-catenin signaling pathway-related proteins.Results:With the increase of Wuzhishan CallicarpanudifloraHook.etArn in Hainan Province concentration, the colony number of SW480 and SW620 cells was down-regulated and the inhibition rate was up-regulated in a dose-dependent manner. Compared with that in control group(con group), the colony size of SW480 and SW620 cells in group Wuzhishan CallicarpanudifloraHook.etArn in Hainan Province group (Lhzz group) decreased, the cell morphology was blurred and the number of colony cells decreased significantly. Compared with that in con group, the green fluorescence intensity of IFITM1 transmembrane protein expression in SW480 and SW620 cells in Lhzz group decreased, the number of colony formation decreased, the volume decreased, and the nucleus showed pyknosis and irregular shape. The expression of tumor suppressor protein DCC in SW480 cells in Lhzz group was significantly higher than that in con group (2.13 ± 0.33 vs. 1.05 ± 0.16, t = 3.542, P < 0.05). The expression level of tumor suppressor protein DCC in SW620 cells in con group was significantly higher than that in con group (1.89 ± 0.26 vs. 0.96 ± 0.12, t = 3.466, P < 0.05). The expression level of oncoprotein DCC in SW620 cells in con group was significantly higher than that in con group (0.64 ± 0.11 vs. 0.38 ± 0.05, t = 3.154, P < 0.05), and the expression level of tumor suppressor protein in SW620 cells in con group was significantly higher than that in con group (0.52 ± 0.09 vs. 0.24 ± 0.03, t = 3.143, P < 0.05), and the expression level of β-catenin in SW480 and SW620 cells in Lhzz group was significantly higher than that in con group ( P < 0.05). Conclusions:Wuzhishan CallicarpanudifloraHook.etArn in Hainan Province can inhibit the proliferation of intestinal cancer cells by activating Wnt/β-catenin signaling pathway.