1.Clinical Effect of Renal Artery Embolization for Renal Graft Dysfunction
Zhihao YANG ; Zhidong YE ; Peng LIU
Chinese Journal of Minimally Invasive Surgery 2001;0(04):-
Objective To explore the clinical effect of renal artery embolization for renal graft dysfunction.Methods A total of 17 patients with renal graft dysfunction were treated by embolizing the renal graft artery.Coil embolization was performed on 7 of the cases,and gelfoam combined with coil was used in the other 10.Results Among the 17 patients,14 discontinued immunosuppressant in 3 weeks after the embolization,and 2 discontinued after receiving low-dose cortisones for about 6 months,and showed good recovery during follow-up.The other patient underwent resection of the renal graft because of continuous fever and graft pain.After the embolization,14 cases developed 'post-embolization syndrome'.Coil migration occurred in 1 case during the procedure.One patient experienced angina during the operation,and developed myocardial infraction the day after the embolization.Conclusions Renal artery embolization is safe and effective for patients with renal graft dysfunction.By using the method,open surgery can be avoided.Attention should be paid to potential complications.
2.Determination of hyperoside in Zhidai tablet by HPLC
Zhihao LI ; Xinjian SUN ; Peng LI
International Journal of Traditional Chinese Medicine 2014;36(2):135-137
Objective To establish a HPLC method for the determination of hyperoside in Zhidai tablet.Method The HPLC system used the Phenomenex LUNA-C18 (4.6 mm× 250 mm,5 μm)column,the mobile phase consisted of acetonitrile (10)-0.1% H3PO4 (90),the flow rate was 1.0 ml/min,the column temperature was set at 30℃,the detection wavelength was set at 360 nm.Results The linear response range was 1.2~24.0 μg/mL(r=0.9998).The average recovery of mangiferin was 98.4%,and RSD was 0.93%.Conclusions The method is simple,rapid,accurate and repeatable.It can be applied in the determination of hyperoside in Zhidai tablet.
3.Determination of lobetyolin in Shengmai Granules by HPLC
Zhihao LI ; Peng LI ; Jin WU ; Xinjian SUN
International Journal of Traditional Chinese Medicine 2011;33(2):134-136
Objective To establish an HPLC method for the determination of lobetyolin in Shengmai Granules. Methods ZORBAX SB-C18(250mm×4.6 mm,5μm) column was used, the mobile phase consisted acetonitrile:0.1% acetic acid (22:78, v/v), the flow rate was 0.8 ml/min, the column temperature was 30℃ and the detecting wavelength was 267 nm. Results The cablibration curve showed good linear relation within a range of 0.082~ 1.640 mg/ml, the average recovery was 98.5% and the RSD was 0.70%.Conclusion The method was simple, repeatable and accurate. It can be applied in quantitative determination of lobetyolin in Shengmai Granules.
4.Determination of hesperidine inErchen mixture by HPLC
Jing LIU ; Peigen HE ; Zhihao LI ; Peng LI
International Journal of Traditional Chinese Medicine 2014;(10):909-911
Objective To establish a method for the determination of hesperidine inErchen mixture. Methods HPLC method was used with Fortis C18(250 mm×4.6 mm,5μm)column and the mobile phase consisted of acetonitrile-acetic acid-water(22∶4∶74). The flow rate was 1.0 ml/min,the column temperature was 30℃, and the UV detector was set at 283 nm.Results The linear ranges of hesperidine was 2.86-57.20μg(r=0.999 8). The average recovery of Aloin was 99.4%.Conclusion The method is rapid, reliable, simple, repeatable and with high specificity. It can be an important supplement for Erchen mixture elevated standard.
5.Research on preparation technology of ligustilide liposomes
Zhihao LI ; Peng LI ; Xuesong ZHU ; Fang ZHENG
Chinese Traditional and Herbal Drugs 1994;0(04):-
Objective To optimize the prescription and preparation technclogy of ligustilide liposomes. Methods Ligustilide liposomes were prepared by ethanol injection method. The encapsulation efficiency was taken as inspection target and the preparation of liposomes was optimized by orthogonal design. HPLC was used to measure the encapsulation efficiency. Results The best prescription was ligustilide-lecithin (1:10),lecithin-cholesterol (2:1),the water phosphate buffer solution (pH 7.5),the hydration temperature was 35 ℃. Conclusion The optimized formulation of ligustilide is reasonable in prescription and practicable in technology.
6.Simultaneous Determination of Chlorogenic Acid and Luteoloside in the Leaves of“Wudang No.II”Flos Lonicerae by HPLC-DAD
Peng LI ; Fang ZHENG ; Cong LI ; Zhihao LI ; Linjie HUANG ; Xuesong ZHU
Herald of Medicine 2015;(5):660-663
Objective To establish a HPLC method for simultaneous determination of chlorogenic acid and luteoloside in the leaves of“Wudang No.II” flos lonicerae. Methods Phenomenex C18(4. 6 mmí250 mm, 5μm) was used;the mobile phase was acetonitrile( A) and 0. 4% phosphoric acid aqueous solution( B) by gradient elution mode; the detection wavelength was 350 nm and the flow rate was 0. 8 mL·min-1;the column temperature was set at 32℃. Results The calibration curve of chlorogenic acid and luteoloside was linear in the range of 0. 285-2. 280μg(r=0. 999 3), and 0. 124-1. 240μg(r=0. 999 4), respectively. The mean recovery of chlorogenic acid and luteoloside was 98. 9%, RSD=1. 59% and 98. 8%, RSD=1. 84%, respectively. Conclusion This method was found to be accurate, quick and reproducible. It can be used for simultaneous determination of chlorogenic acid and luteoloside in the leaves of “Wudang NO.II”flos lonicerae.
7.Study macroporous adsorptive resins on purification technology of polysaccharides from Lonicera japonica Thunb
Zhihao LI ; Yinhua CHEN ; Peng LI ; Fang ZHENG ; Jun ZHU ; Linjie HUANG ; Cong LI
International Journal of Traditional Chinese Medicine 2015;(4):345-348
Objective To study the effect of macroporous adsorptive resins on the decoloration technology ofLonicera japonica Thunb. polysaccharides(FLP).Methods The effects of 6 kinds of macroporous adsorptive resins i.e. HPD-400A, AB-8, HPD-750, HPD-100, D3520, D301T, S8 on the decolorization technology ofLonicera japonica Thunb. polysaccharides were compared with single factor test in terms of temperature, polysaccharide concentration, pH, adsorption flow, and eluant.Results The decolorization rate and polysaccharide retention rate of S8 were optimal. The best decoloration conditions were as follows: temperature of 40℃, polysaccharide concentration of 5 mg/ml, pH value of 6, flow rate of 1 ml/min, distilled water with pH=6 as eluant. The adsorption rate was 83.2%,and polysaccharide retention rate was 72.1%.Conclusion High decolorization ratio and the high retention rate ofLonicera japonica Thunb. could be obtained by means of decoloration with S8 macroporous adsorptive resins..
8.Effect of Low Dose of Chicken Infectious Anemia Virus in Attenuated Vaccine on SPF Chicken Body Weight and Vaccine Immune Antibody.
Lichun FANG ; Xiaohan LI ; Zhihao REN ; Yang LI ; Yixin WANG ; Zhizhong CUI ; Shuang CHANG ; Peng ZHAO
Chinese Journal of Virology 2016;32(2):190-194
In order to observe the effect of the immune and weight of chickens after use the attenuated vaccine with low dose of chicken infectious anemia virus (CIAV). In this study, the effects of low dose of CIAV on the weight of SPF chickens and NDV antibody production were observed by simulated experiments. The results showed that 10 EID50 and 5 EID50 CIAV per plume attenuated NDV vaccines were used to cause the weight loss of SPF chickens. Compared with the use of the non contaminated vaccine group, it has significant difference. And NDV antibody levels compared with the use of the non contaminated groups also decreased after use the vaccine with two doses of CIAV contaminated. It has significant difference. A certain proportion of CIAV antibody positive was detected at the beginning of the second week after use the NDV vaccine with two doses of CIAV contaminated. The detection of a high proportion of CIAV nucleic acid was detected in the first week after the use of a contaminated vaccine. The results of the study demonstrate the effects of CIAV pollution on the production and immune function of SPF chickens, and it is suggested that increasing the detection of viral nucleic acid can help save time and improve the detection rate in the detection of exogenous virus contamination by SPF chicken test method.
Animals
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Antibodies, Viral
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immunology
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Chicken anemia virus
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genetics
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immunology
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physiology
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Chickens
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Circoviridae Infections
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immunology
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veterinary
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virology
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Poultry Diseases
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immunology
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virology
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Specific Pathogen-Free Organisms
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Vaccines, Attenuated
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administration & dosage
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genetics
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immunology
9.Application of case three-dimensional teaching method combined with standardized patient in the clinical clerkship teaching reform of gastrointestinal surgery
Mingkai ZHANG ; Haitao GENG ; Jianping WANG ; Zhihao PENG ; Yanbin LI ; Yuming LI
Chinese Journal of Medical Education Research 2021;20(2):196-198
Objective:To explore the application value of case three-dimensional teaching method combined with standardized patient (CTTM+SP) in the clinical clerkship teaching reform of gastrointestinal surgery.Methods:A total of 120 students of clinical medicine having clerkship in Binzhou Medical University Hospital were recruited in this study, and they were randomized to the experimental group and the control group, with 60 students in each group. The experimental group took the CTTM+SP teaching method, and the control group adopted the lecture-based learning teaching method. The comprehensive ability of the two groups was evaluated by department graduation examination and satisfaction survey.Results:The examination performance and the satisfaction rate of students in the experimental group were significantly higher than those of the control group ( P < 0.05). Conclusion:CTTM+SP teaching method is helpful for the probationers to master the theoretical knowledge of gastrointestinal surgery systematically, improve the clinical thinking ability and the ability to solve practical problems, mobilize the students' learning motivation and interest, and improve the teaching effect of gastrointestinal surgery clinical clerkship.
10. Minimally invasive plate oseosynthesis assisted by a self-designed lower limb axial tractor for treatment of complex tibial plateau fractures
Haolong YUAN ; Zhigang WANG ; Long JIA ; Zhaolin WANG ; Kai ZHANG ; Gangqiang DU ; Peng LI ; Shengyuan JIANG ; Zhihao GONG
Chinese Journal of Orthopaedic Trauma 2019;21(11):959-965
Objective:
To investigate the clinical efficacy of minimally invasive plate oseoynthesis (MIPO) assisted by a self-designed lower limb axial tractor in the treatment of complex tibial plateau fractures.
Methods:
The data were retrospectively analyzed of the 31 patients who had undergone surgical treatment for complex tibial plateau fractures from June 2015 to April 2018 at Department of Orthopeadics, Hospital Affiliated to Binzhou Medical College. MIPO was conducted with the assistance of the axial tractor in 17 of them. They were 12 men and 5 women with an age of 50.8±14.7 years, with 8 cases of Schatzker type Ⅴ and 9 ones of Schatzker type Ⅵ. MIPO was conducted without the assistance of the axial tractor but with the assistance of manipulation reduction in the other 14 patients. They were 9 men and 5 women with an age of 48.9±9.5 years, with 6 cases of Schatzker type Ⅴ and 8 ones of Schatzker type Ⅵ. The 2 groups were compared in terms of operation time, intraoperative bleeding, and Rasmussen scores for knee function and medial angle of the proximal tibia at the last follow-up.
Results:
The 2 groups were comparable due to insignificant differences in the preoperative general data between them (