This experiment was made on 4-6 week-old nude mice(NM). The CNE-2 cells were inoculated subcutaneously into each side of the neckback for the original generation. Implanted fresh transplanted NPC tissue to NM's neck-back with cannula for next passage. We passed up to 8 generations and the transplantability was 100%. We observed dynamicrly the changes of NM's immune status after they have bearing the NPC as following: (1) The net weight was significantly reduced. (2) Splenic index(S. I.) and tumor weight(T. W.) increased. S. I. was highly related to T. W. (r=0.8395). (3) Splenic NK activity slightly increased in 5 days, began to decrease in 10 days and significantly decreased in 15 or more days. NK activity was negative related to T. W. and S. I. markedly (r=-0.7132, r=-0.8237). (4) Splenic NK cells had excellent responsiveness to human IFN in vitro in 5 days and still had some in 10 days, but it had not any after 15 days. (5) Natural proliferative ratio of tumor bearing NM's splenic cells was higher than that of control group, but the responsiveness to ConA was much lower. (6) Ai Ke Xing suppressed the growth of transplanted NPC by means of decreasing sera MDA enhancing host's splenic NK activity and increasing mononuclear infiltration of the transplanted NPC tissue. So we concluded that in NM whose T cells were deficient splenic NK cells were important immunocompetent cells for resisting the growth of transplanted NPC.

Objective To develop and test a stressor scale for in-patients with spinal cord injury (SSIPSCI). Methods Based on in-depth interviews and the second-level International Classification of Functioning, Disability, and Health (ICF) categories, a 39-item SSIPSCI was generated and pilot tested. It was then used with 284 inpatients with spinal cord injury. Principal components factor analysis and correlation analysis were performed.Results The exploratory components factor analysis revealed 7 significant stress factors: social activity, economy security, individual activity, relationship with others, physical disorders, bowel and bladder problems and medical fees. Internal consistency was high, with a Cronbach's alpha coefficient of 0. 950 overall and 0. 741 ～ 0. 920 for the individual factors. Test-retest reliability was 0.848. The correlation between SSIPSCI scores and Beck depression inventory (BDI) scores was strong ( r = 0. 665 ). Conclusions The reliability and validity of the SSIPSCI are both satisfactory enough to meet psychometric requirements.

In recent years, studies have shown that transplanted neural stem cells (NSCs) help neural tissues regenerate and return to normal through paracrine action rather than just replacing cells. Exosomes are essential paracrine mediators that can participate in cell communication through substance transmission. This review focuses on NSCs regulated by exosomes and their application in treatment of nervous system diseases, in order to provide important references for further research and clinical application of NSCs exosomes..