Objective:To monitor the effects of asarone injections on the expression of IL-25, IL-27 and eosinophils cationic pro-tein ( ECP) in the serum of asthma patients. Methods:Totally 100 patients with bronchial asthma were randomly divided into the ob-servation group ( 55 cases ) and the control group ( 45 cases ) . The control group was treated with the conventional symptomatic treat-ment ( oxygen inhalation, spasmolysis and anti-asthma, and anti-infection, etc) . The observation group was given asarone injections 24 mg in 250 ml 0. 9% sodium chloride infusions, once a day for 14 days on the basis of the conventional therapy. The changes in symp-toms, and pulmonary function in the patients were observed with simultaneous detection of IL-25, ECP and IL-27 levels. Results:To-tal effective rate of the observation group was 92. 7%,which was higher than that of the control group (P<0. 05). Asarone injections could decrease IL-25, ECP and IL-27 levels in the observation group compared with those in the control group with statistically signifi-cant difference (P<0. 05 or 0. 01). Conclusion:Asarone injections can decrease the levels of IL-25, IL-27and ECP in the serum of asthma patients and inhibit the formation of inflammatory cells to alleviate the symptoms of airway inflammation.

Complexity algorithm and wavelet package transform are discussed in this paper. To improve the classification ability of complexity algorithm in motion pattern recognition, it's used together with wavelet package transform. This method shows a better performance than a single complexity algorithm.

ObjectiveTo study the effect of heat stress on the cytoskeleton and cell cycle of human umbilical vein endothelial cell (HUVEC) in vitro.Methods HUVEC was cultured in vitro in 5%CO2 medium at 37℃ (control group) or 43℃ (heat stress group) for 1 hour. Coomassie brilliant blue R-250 staining was used to determine the effect of heat stress on the cytoskeleton. The cells in heat stress group were subsequently cultured at 37℃in 5%CO2 medium after heat stress for 1 hour, and cell cycle of HUVEC was determined at 0, 6, 12, 18 and 24 hours with flow cytometry.Results Under light microscopy normal cytoskeleton was observed in control group, but thicker and shorter cytoskeleton was found after a rise of temperature, and stress fibers were found in heat stress group. The DNA content of HUVEC at all time points in G0/G1 stage was 38.07%-55.19% after heat stress. The DNA content in control group was 48.57%, and it was 54.06%, 55.19%, 48.23%, 38.07%, and 41.03% at 0, 6, 12, 18, 24 hours in G0/G1 stage in heat stress group. DNA content in S phase was 35.33%-48.18%. The DNA content in control group was 44.62%, and it was 35.33%, 39.50%, 42.50%, 48.18%, and 47.99% at 0, 6, 12, 18, 24 hours in S stage in heat stress group. DNA content in G2/M phase was 5.31%-13.75%. The DNA content in control group was 6.81, and it was 10.61%, 5.31%, 9.27%,13.75%, and 10.98% at 0, 6, 12, 18, 24 hours in G2/M stage in heat stress group. It was demonstrated that compared with control group, the DNA content in G0/G1 stage was significantly increased when the HUVEC were separated from heat stress within 6 hours, and it recovered at a similar level as control group at 12 hours.Conclusion Heat stress can change the cytoskeleton of HUVEC, and cause stagnation at G0/G1 stage in cell cycle.

Objective To know the current application status of reference interval of cystatin C andβ2-microglobulin in medi-cal institutions of China.Methods Every laboratory which attended 2014 specific protein external quality assessment pro-gram of national center of clinical laboratories has been received an internet questionnaire to survey reference intervals of cystatin C andβ2-microglobulin.After eliminating outliers the trimmed data was statistical analyzed by Microsoft Excel 2007 and SPSS 13.0.The resources of RIs,traditional statistical analysis,normalized analysis,comparison between overall median and lower and upper limits of different measurement system,and verification status of RIs were included.Results 106 and 127 medical laboratories submitted the questionnaire and the number of province with submission of RIs for the two renal function measurement items were 24 and 29,respectively.Less than 5 laboratories grouped their RIs according to age and/or gender.Approximately 75% of laboratories had been using the RIs referring to reagent manufacturers’instructions.All me-dians of upper or lower limits of analytes were as same as the Mode values of the same group.The 2.5th percentiles had tre-mendous differences compared with 97.5th percentiles.More than half of laboratories did not verify their RIs beforeusing them.The overall medians of lower and upper limits of RIs were (in serum):Cystatin C (mg/L)0.55 and 1.09;β2-micro-globulin (μg/ml)1.00 and 2.80.Conclusion The RIs of cystatin C and β2-microglobulin used in medical institution had some differences between each other,and there were too many kinds of reagents and instruments which made different meas-urement systems.The consistent and standardized reference intervals should be established in a short time.The RI for cysta-tin C was 0.55~1.09 mg/L,forβ2-microglobulin was 1.00~2.80 μg/ml considered by this study.

Objective To analyze the results of external quality assessment (EQA) and internal quality control (IQC) of first trimester maternal serum prenatal screening,and to investigate methods to improve the quality of prenatal screening.Methods Based on the reagent,laboratories which participated in the EQA project by National Center for Clinical Laboratories in May and October in 2013 were divided into PerkinElmer (PE) group (n=44) and Roche group (n=14).Five human serum quality control samples were distributed to each laboratory in every session to test free β-human chorionic gonadotropin (β-hCG) and pregnancy associated plasma protein A (PAPP-A).The M,P75 and P25 of each sample were calculated.The robust coefficient of variation (CV) was defined as [0.7413 × (P75-P25)]/M× 100％.In five samples of each EQA session,if the results of four or more samples were within the M (1± 30％),the laboratory passed the session.If the laboratory passed both sessions,the laboratory passed the EQA project of 2013.The CV of in control quality control and cumulative CV of in control quality control results in May 2013 session were gathered.Results (1) Results of EQA:The robust CVs of all samples of two sessions were 5.0％-7.2％ for free β-hCG in PE group,and 9.5％-188.4％ in Roche group.The robust CVs were 6.4％-116.9％ for PAPP-A in PE group,and 10.5％-165.3％ in Roche group.Fifty five (94.8％,55/58) laboratories passed the EQA project of 2013 for free β-hCG.Forty four (75.8％,44/58) laboratories passed the EQA project of 2013 for PAPP-A.(2) Results of IQC:Twenty eight and twenty six laboratories reported IQC results for free β-hCG and PAPP-A respectively.In eighteen (55.1％,18/28) and fourteen (50.0％,14/28) laboratories,the CV of in control quality control and cumulative CV of in control quality control results for free β-hCG were less than 5％ respectively.In Fifteen (57.7％,15/26) and thirteen (50.0％,13/26) laboratories,the CV of in control quality control and cumulative CV of in control quality control results for PAPP-A were less than 5％ respectively.Conclusions There are differences of the testing quality for first trimester maternal serum prenatal screening in China.EQA and IQC can help laboratories to find problems,improve quality and maintain the testing consistency.

Objective To calculate and describe the decision limits of 2014 external quality assessment(EQA) programs in D-Dimer of 225 labs.Methods To collectedthe information of decision limits which come from the 2014 External Quality Assessment ( EQA) programs in D-Dimer.All data from maternal and child care service centers , children′s hospitals and corporations were excluded .Then all the abnormal values and errors were eliminated either .Data of 225 labs statistics were processed by Microsoft Excel 2007, which include the mean, median, maximum value, minimum value, P2.5 and P97.5.Weanalyzed the sources of decision limits and then get the data filtered .The reagent manufacturer 's instructions data are reservedas a reference source and data are analyzed according to differentreagents .Results According to the surveys of decision limits sources ,most of thereference sources use the reagent manufacturer′s instructions. According to the surveys,the mean and median of each test project is close .The P2.5 and P97.5 distribution ranges are: D-Dimer(DDU) 0.3 -1.33 μg/ml,D-Dimer(FEU) 0.23 -1.50 μg/ml,with significant differences.According to the surveys , most P2.5 and P97.5 reagent distributions are significantly different . Conclusion The decision limitsof the mean and median of each test item are approximate ,and the P2.5 and P97.5 distributions of each test item are poorly consistent .

Objective To study the applicability of a new analytical specification defined in WS/T 403-2012 in the external quality assessment schemes and the external comparision of internal quality control .Methods It was a quality management method study.Total error allowable criterions listed in WS/T 403-2012 and GB/T 20470-2006 were selected to assess the results of 23 analytes in the 1st challenge of 2013 routine chemistry external quality assessment.The acceptable rate of 23 analytes were calculated with the two specifications.Criterions of imprecision derived from the two standards were applied to assess the coefficient of variation with internal quality control data.Results With the specification based on WS/T 403-2012, the ratio of laboratories that all five samples were passed in the 1st challenge for 23 analytes ranged from 55.5%to 94.7%.The ratio of laboratories with 80%or more samples passed in 2013 EQA ranged from 73.9%to 98.5%.While ratios of two kinds described above evaluated based on GB /T 20470-2006 ranged from 63.0%to 99.2%, and from 90.0% to 99.7%, respectively.The acceptable rate of CV according to the two criterions ranged from 55.5% to 94.7% and 63.0% to 99.2%, respectively.Conclusions As evaluation criterions of external quality assessment allowable total error and internal quality control imprecision in routine chemistry , the specification in WS/T 403-2012 can be used to assess the analytical performance of clinical laboratory more objectively and comprehensively.It can help laboratories to identify the latent problems for further quality improvement.

OBJECTIVE:To observe the efficacy and safety of breviscapine combined with double shock in the treatment of lu-pus encephalopathy. METHODS:70 patients with lupus encephalopathy were randomly divided into control group and observation group. Control group was treated with Methylprednisolone sodium succinate for injection 500 mg for continuous 3 d,iv,once a day,Cyclophosphamide for injection 1.0 g in the 4th day,iv,and Methylprednisolone sodium succinate for injection 80 mg was used for maintaining treatment in the 5th day for continuous 15 d,once a day. Observation group was treated with Breviscapine for injection 50 mg for continuous 20 d based on the treatment of control group,iv,once a day. The SLE activity(SLEDAI) score, IL-1β,IL-6,IL-8 and IFN-γ were observed,cases of neurological and psychological symptoms,the fatality rate and incidence of adverse reactions were recorded. RESULTS:After treatment,the SLEDAI,IL-1β,IL-6,IL-8 and IFN-γ in 2 groups were signifi-cantly lower than before,and observation group was lower than control group(P<0.05);the cases of neurological symptoms(ex-cept for aseptic meningitis) and psychological symptoms (except for auditory hallucinations) were significantly less than control group(P<0.05). There was no significant difference in the mortality rate and incidence of adverse reactions(P>0.05). CONCLU-SIONS:Breviscapine combined with double shock has good efficacy and safety in the treatment of lupus encephalopathy,it can re-duce the SLEDAI score,improve neurological and psychological symptoms.

Objective To investigate the protective effect of heat shock protein (HSP) 70 on the acute lung injury (ALI) of rats with heat stroke.Methods Sixty four rats were randomly (by employing a random number table) assigned into a sham-heated group (Sham group),heat stress group (HS group),and HS plus gluttamine treatment group (HS+GLN group) and HS plus quercet in treatment group (HS+QU group),16 each.All rats were housed in a artificial climate chamber,with the rats in the sham groups exposed to a temperature of 23 ℃ and humidity of 55％ ± 5％,while the rats of HS,HS+GLN and HS+QU groups to an ambient temperature of 39 ℃ and humidity of 65％.During heat stress or sham heating,rectal temperature (Tr),systolic blood pressure (SBP) and pulse rate (PR) were monitored to observe the difference in heat stress response among the groups.The time point at which the SBP started to drop from the peak level was taken as the point of HS onset.At the onset of HS,heat exposure was terminated,then the rats were immediately removed from the chamber,and returned to room temperature.The rats were scarified 0h and 6h after HS onset respectively.After bronchoalveolar lavage fluid (BALF) was collected,the lungs of all animals were harvested for pathological examination of lung injury.The concentrations of IL-1β,TNF-α and IL-6 in BALF and HSP70 in lung homogenate were measured by using an enzyme linked immunosorbent assay kit.Results Compared with HS and HS+QU groups,the rats in HS+GLN group required significantly greater heat load to induce HS (P＜0.001),and had longer survival time span after HS onset.Compared with Sham group,the concentration of HSP70 in lung homogenate in HS group increased in a time-dependent manner (P＜0.001).In comparison with HS group,the concentration of HSP70 in lung homogenate from HS+GLN group was significantly elevated at each time point (P＜0.001),while the treatment with QU significantly inhibited the expression of HSP70 (P＜0.001).The concentration of IL-1β,TNF-α and IL-6 in BALF significantly decreased in HS+GLN group compared with those in HS group and HS+QU group (P＜0.001).The pathological results showed that the lung injury was milder in HS+GLN group,while the opposite in HS+QU group.Conclusion HSP70 could protect HS rats against ALI by enhancing their thermo-tolerance and inhibiting inflammatory response.

Objective To investigate the role of oxidative stress in acute liver injury in a heat stroke model of conscious rats,and to explore its underlying mechanism.Methods Thirty-two rats were randomly (by using a random number table) assigned into a sham-heated control group (Sham group,n=8),a sham-heated group treated with NAC (Sham-NAC group,n=8),a heat stroke group (HS group,n=8) and a heat stoke group treated with NAC (HS-NAC,n=8).Rats were prepared with pre-warm chamber to initiate heat stoke.The change of rectum temperature (Tr),heart rate (HR) and systolic blood pressure (SBP) were monitored,and the time point of HS onset was recorded.Rats were sacrificed 12h after HS onset.ALT,serum TBIL,IL-6,IL-1β,TNF-α,MDA,T-SOD and GSH in the liver homogenates were measured.Liver tissues were harvested for determining the concentration of reactive oxygen species (ROS),neutrophil infiltration and the histological changes.Results During HS onset,no significant differences were observed in Tr,HR,SBP and heat exposure time between HS group and HS-NAC group (P＞0.05).However,the survival time was significantly longer in HS-NAC group than in HS group (P=0.039).12 hours after HS onset,the concentrations of ROS and MDA in the liver homogenates were significantly higher in HS group than in the other groups (P=0.000),while the concentrations of T-SOD and GSH were much lower than in the other groups (P=0.000).The serum concentrations of ALT and TBIL were significantly higher in HS group than in the other groups (P=0.000).Compare with HS group,the pathological injury was alleviated in HS-NAC group (P=0.000).The neutrophil infiltration level and the concentrations of IL-6,IL-1 β and TNF-α in liver tissue were significantly higher in HS group than in HS-NAC group (P=0.000).Conclusion Oxidative stress may play an important role in the pathogenesis of HS liver injury through its cytotoxic effect and by inducing inflammatory responses.