Objective:To monitor the effects of asarone injections on the expression of IL-25, IL-27 and eosinophils cationic pro-tein ( ECP) in the serum of asthma patients. Methods:Totally 100 patients with bronchial asthma were randomly divided into the ob-servation group ( 55 cases ) and the control group ( 45 cases ) . The control group was treated with the conventional symptomatic treat-ment ( oxygen inhalation, spasmolysis and anti-asthma, and anti-infection, etc) . The observation group was given asarone injections 24 mg in 250 ml 0. 9% sodium chloride infusions, once a day for 14 days on the basis of the conventional therapy. The changes in symp-toms, and pulmonary function in the patients were observed with simultaneous detection of IL-25, ECP and IL-27 levels. Results:To-tal effective rate of the observation group was 92. 7%,which was higher than that of the control group (P<0. 05). Asarone injections could decrease IL-25, ECP and IL-27 levels in the observation group compared with those in the control group with statistically signifi-cant difference (P<0. 05 or 0. 01). Conclusion:Asarone injections can decrease the levels of IL-25, IL-27and ECP in the serum of asthma patients and inhibit the formation of inflammatory cells to alleviate the symptoms of airway inflammation.

Complexity algorithm and wavelet package transform are discussed in this paper. To improve the classification ability of complexity algorithm in motion pattern recognition, it's used together with wavelet package transform. This method shows a better performance than a single complexity algorithm.

ObjectiveTo study the effect of heat stress on the cytoskeleton and cell cycle of human umbilical vein endothelial cell (HUVEC) in vitro.Methods HUVEC was cultured in vitro in 5%CO2 medium at 37℃ (control group) or 43℃ (heat stress group) for 1 hour. Coomassie brilliant blue R-250 staining was used to determine the effect of heat stress on the cytoskeleton. The cells in heat stress group were subsequently cultured at 37℃in 5%CO2 medium after heat stress for 1 hour, and cell cycle of HUVEC was determined at 0, 6, 12, 18 and 24 hours with flow cytometry.Results Under light microscopy normal cytoskeleton was observed in control group, but thicker and shorter cytoskeleton was found after a rise of temperature, and stress fibers were found in heat stress group. The DNA content of HUVEC at all time points in G0/G1 stage was 38.07%-55.19% after heat stress. The DNA content in control group was 48.57%, and it was 54.06%, 55.19%, 48.23%, 38.07%, and 41.03% at 0, 6, 12, 18, 24 hours in G0/G1 stage in heat stress group. DNA content in S phase was 35.33%-48.18%. The DNA content in control group was 44.62%, and it was 35.33%, 39.50%, 42.50%, 48.18%, and 47.99% at 0, 6, 12, 18, 24 hours in S stage in heat stress group. DNA content in G2/M phase was 5.31%-13.75%. The DNA content in control group was 6.81, and it was 10.61%, 5.31%, 9.27%,13.75%, and 10.98% at 0, 6, 12, 18, 24 hours in G2/M stage in heat stress group. It was demonstrated that compared with control group, the DNA content in G0/G1 stage was significantly increased when the HUVEC were separated from heat stress within 6 hours, and it recovered at a similar level as control group at 12 hours.Conclusion Heat stress can change the cytoskeleton of HUVEC, and cause stagnation at G0/G1 stage in cell cycle.

Objective To know the current application status of reference interval of cystatin C andβ2-microglobulin in medi-cal institutions of China.Methods Every laboratory which attended 2014 specific protein external quality assessment pro-gram of national center of clinical laboratories has been received an internet questionnaire to survey reference intervals of cystatin C andβ2-microglobulin.After eliminating outliers the trimmed data was statistical analyzed by Microsoft Excel 2007 and SPSS 13.0.The resources of RIs,traditional statistical analysis,normalized analysis,comparison between overall median and lower and upper limits of different measurement system,and verification status of RIs were included.Results 106 and 127 medical laboratories submitted the questionnaire and the number of province with submission of RIs for the two renal function measurement items were 24 and 29,respectively.Less than 5 laboratories grouped their RIs according to age and/or gender.Approximately 75% of laboratories had been using the RIs referring to reagent manufacturers’instructions.All me-dians of upper or lower limits of analytes were as same as the Mode values of the same group.The 2.5th percentiles had tre-mendous differences compared with 97.5th percentiles.More than half of laboratories did not verify their RIs beforeusing them.The overall medians of lower and upper limits of RIs were (in serum):Cystatin C (mg/L)0.55 and 1.09;β2-micro-globulin (μg/ml)1.00 and 2.80.Conclusion The RIs of cystatin C and β2-microglobulin used in medical institution had some differences between each other,and there were too many kinds of reagents and instruments which made different meas-urement systems.The consistent and standardized reference intervals should be established in a short time.The RI for cysta-tin C was 0.55~1.09 mg/L,forβ2-microglobulin was 1.00~2.80 μg/ml considered by this study.

Objective To analyze the results of external quality assessment (EQA) and internal quality control (IQC) of first trimester maternal serum prenatal screening,and to investigate methods to improve the quality of prenatal screening.Methods Based on the reagent,laboratories which participated in the EQA project by National Center for Clinical Laboratories in May and October in 2013 were divided into PerkinElmer (PE) group (n=44) and Roche group (n=14).Five human serum quality control samples were distributed to each laboratory in every session to test free β-human chorionic gonadotropin (β-hCG) and pregnancy associated plasma protein A (PAPP-A).The M,P75 and P25 of each sample were calculated.The robust coefficient of variation (CV) was defined as [0.7413 × (P75-P25)]/M× 100％.In five samples of each EQA session,if the results of four or more samples were within the M (1± 30％),the laboratory passed the session.If the laboratory passed both sessions,the laboratory passed the EQA project of 2013.The CV of in control quality control and cumulative CV of in control quality control results in May 2013 session were gathered.Results (1) Results of EQA:The robust CVs of all samples of two sessions were 5.0％-7.2％ for free β-hCG in PE group,and 9.5％-188.4％ in Roche group.The robust CVs were 6.4％-116.9％ for PAPP-A in PE group,and 10.5％-165.3％ in Roche group.Fifty five (94.8％,55/58) laboratories passed the EQA project of 2013 for free β-hCG.Forty four (75.8％,44/58) laboratories passed the EQA project of 2013 for PAPP-A.(2) Results of IQC:Twenty eight and twenty six laboratories reported IQC results for free β-hCG and PAPP-A respectively.In eighteen (55.1％,18/28) and fourteen (50.0％,14/28) laboratories,the CV of in control quality control and cumulative CV of in control quality control results for free β-hCG were less than 5％ respectively.In Fifteen (57.7％,15/26) and thirteen (50.0％,13/26) laboratories,the CV of in control quality control and cumulative CV of in control quality control results for PAPP-A were less than 5％ respectively.Conclusions There are differences of the testing quality for first trimester maternal serum prenatal screening in China.EQA and IQC can help laboratories to find problems,improve quality and maintain the testing consistency.

Objective To calculate and describe the decision limits of 2014 external quality assessment(EQA) programs in D-Dimer of 225 labs.Methods To collectedthe information of decision limits which come from the 2014 External Quality Assessment ( EQA) programs in D-Dimer.All data from maternal and child care service centers , children′s hospitals and corporations were excluded .Then all the abnormal values and errors were eliminated either .Data of 225 labs statistics were processed by Microsoft Excel 2007, which include the mean, median, maximum value, minimum value, P2.5 and P97.5.Weanalyzed the sources of decision limits and then get the data filtered .The reagent manufacturer 's instructions data are reservedas a reference source and data are analyzed according to differentreagents .Results According to the surveys of decision limits sources ,most of thereference sources use the reagent manufacturer′s instructions. According to the surveys,the mean and median of each test project is close .The P2.5 and P97.5 distribution ranges are: D-Dimer(DDU) 0.3 -1.33 μg/ml,D-Dimer(FEU) 0.23 -1.50 μg/ml,with significant differences.According to the surveys , most P2.5 and P97.5 reagent distributions are significantly different . Conclusion The decision limitsof the mean and median of each test item are approximate ,and the P2.5 and P97.5 distributions of each test item are poorly consistent .

Objective To investigate the functional changes of C3A cells co-cultured with pancreatic endothelial cells. Methods C3A cells and pancreatic endothelial cells lwere cultivated directly and indirectly for 7 days by the density ratio of 10: 1. Meanwhile, the blank control was established.The growth and morphological characteristics of experimental groups were observed. The out leakage level of AST, ALT and the synthesis level of albumin and the diazepam metabolism level were determined by automatic biochemistry analyzer, radioimmunoassay and enzyme linked immunosorbent assay. Results Direct co-cultivation of C3A cells and pancreatic endothelial cells could reduce the out leakage of ALT and AST in the 5th and 7th d. The capacity of albumin synthesis could reach 12. 977μg/ml on the 7th d and the that of diazepam metabolism could also be improved. Indirect co-cultivation of C3A cells and pancreatic endothelial cells could significantly raise the albumin synthesis to 7. 380 μg/ml and 10. 773 μg/ml on the 5th and 7th d, respectively. Conclusion Co-cultivation of C3A cells and pancreatic endothelial cells can significantly improve the basic biological function of C3A cells.

Objective To evaluate the effect of low-dose colonic CT scanning in image quality and lesion revealing with different noise idex.Methods Model group:in order to find the optimal scanning parameter range,small simulated polyps in pig colon was prepared and mA was modulated by regulating noise index.Images were reprocessed with CT colonography and Raysum reconstructions.Image quality and the lesion revealing situation were evaluated and scored based on both reconstructive and axial images.The study of patient group was prospective and continuous.All positive cases were comfirmed by biopsy or surgery pathology.Two groups of patients were enrolled.First group(39 cases from January 2007 to June 2007):patients were divided into three sub-groups randomly(15 cages with NI=10,13 cases with NI:13,11 cases with NI=16),while NI was not changed with position.Second group(32 cases from Jury 2007 to January 2008):patients were scanned and NI was changed with different position(NI=10,20).The average X-ray radiation values of two patient groups were compared with standard values.All data were processed with rank sum tesL Results First group:when NI=10,the average scores of axial and constructive images were 4.2 and 2.4 respectively,when NI=13,those were 3.2 and 2.5 respectively,When NI=16.those were 2.9 and 2.7 respectively.and the average CTDlw were 17.51,12.90 and 5.94 mGy respectively.When NI increased,the average scores of axial decreased(H=20.01,P＜0.01),the average scores of constructive images did not changed(H=0.81,P＞0.05).Second group:when NI=10,the average scores of axial and constructive images were respectively 3.6 and 2.3,when NI=20,those were 2.2 and 2.3 respectively,and the average CTDIw was 11.63 mGy.When NI increased from 10 to 20,the average scores of axial decreased(H=20.84,P＜0.01),the average scores of constructive images did not decreased(H=0.29,P＞0.05).In the first group,the average CTDIw wag reduced by 35.0%,52.1%and 77.9%respectively when NI was 10,13 and 16.In the second group,the average CTDIw Was reduced by 56.8%.Conclusions Auto-mA low-dose CT scanning could reduce X-ray radiation obviously,while not affecting image quality.

Objective:Immunoregulation study of umbilical mesenchymal stem cell (UCMSCs) on allogeneic umbilical cord blood(UCB) CD4+T lymphocytes,which proliferation,apoptosis and the differentiation to CD4+CD25+ regulatory T cell (Treg) in vitro. Methods:Establishing on direct contact or transwell co-culture system,adopt in different proportion of UCMCs with phytohaemag-glutinin (PHA)-activated UCB CD4+T lymphocytes were co-cultured. The proliferation of lymphocyte,percent of CD4+CD25+/CD4+and Foxp3 expression, regulatory T cell marker gene were measured. Apoptosis of CD4+T lymphocytes were observed in the direct contact or transwell coculture system of UCMSCs with desamethason( DXM)-stimulated UCB CD4+T lymphocytes. Results: The UCB CD4+T lymphocytes cocultured with UCMSCs with PHA-activating for 3 days,compared with the UCMSCs free control group,the amount of cells was reduced noticeably(P<0. 05) and the percent of CD4+CD25+in CD4+T lymphocytes and Foxp3 expression significantly in-creased(P<0. 01) in a dose dependent way(P<0. 05). The UCB CD4+T lymphocytes cocultured with UCMSCs with DXM-inducing for 7 days,the apoptosis rate was significantly lower than that of the control group without UCMSCs (P<0. 01). These effects were partially attenuated in transwell coculture but could not be eliminated. Conclusion: UCMSCs are negative effect on UCB CD4+T lymphocytes-mediated immunity effects,and mainly manifested in the regulation on cell proliferate ability and differentiation rather than promoting apoptosis.

AIM: To investigate the role of staphylococcal enterotoxin B (SEB) in early intestinal injury in scald rats with Staphylococcus aureus sepsis. METHODS: 86 male Wistar rats were randomly divided into four groups as follows: normal controls ( n= 10), scald control group( n= 10), postburn sepsis group( n= 50) and SEB monoclonal antibody (MAb)treatment group ( n= 16). Plasma samples were collected to determine SEB, endotoxin, tumor necrosis factor-? (TNF-?) and interferon-? (IFN-?). RESULTS: After scald injury followed by Staphylococcus aureus challenge, the levels of SEB, TNF-? and IFN-? in plasma were significantly higher than those of normal controls, peaking at 2-6 h ( P