Objective To study the relationship among the area of transverse section of radial artery, the major axis length of the ellipse with same centroid as transverse section, the maximum centroid displacement in the direction parallel with skin surface in a cardiac cycle and width of pulse tracing. Methods ECG, pressure phygmograms and ultrasound images of transverse section of radial artery of 40 patients with different width of pulse tracing and 20 healthy persons were collected synchronously with a new-type sphymograph. On the basis of images analysis with MATLAB 7.0.1, image characteristics of transverse section of radial artery and centroid motion locus of pulse tracings with different width in a cardiac cycle were observed and analyzed. Results Between pulse tracings with different width, there were significant differences in the area of transverse section of radial artery, the major axis length of the ellipse with same centroid as transverse section and the maximum centroid displacement in the direction parallel with skin surface in a cardiac cycle. Conclusions The mean, maximum and minimum value of the area of transverse section and the major axis length of ellipse and the maximum centroid displacement can be used to discriminate width of pulse tracing.

Objective:To discuss the effect and mechanism of autophagy inhibitor 3-MA on arsenic trioxide inducing apoptosis of acute T-cell leukemia cell line Jurkat cells.Methods:Proliferation inhibition of Jurkat cells treated with arsenic trioxide was detected by XTT.Morphological characteristics of Jurkat cells treated with different concentrations arsenic trioxide were observed by electron mi-croscope.Microtubule-associated protein 1 light chain 3B (LC-3B) protein expression was detected by Western blot and flow cytome-try.Apoptosis rates of Jurkat cells treated with 3-MA combining arsenic trioxide were detected by flow cytometry using AnnexinV-FITC/PI double staining.Results:Arsenic trioxide inhibited the growth of Jurkat cells in a dose and time dependence.We observed different morphological characteristics of autophagy , apoptosis and necrosis accompanying more autophagosomes in Jurkat cells which were treated with arsenic trioxide 2.5,5,10 μmol/L after 24 h.LC3B mean fluorescence intensity (MFI)relative multiples were(3.1±0.2) fold,(4.6±0.31)fold,(34.2±4.5)fold with 5 μmol/L arsenic trioxide treated Jurkat cells 0,24,48 h,and the P values between each of the two groups were less than 0.05,which increased depending time consistently with the growth inhibition rates.LC-3B protein expression gradually increased treated Jurkat cells with arsenic trioxide after 24 h,48 h.The growth inhibition rate (60.6±8.3)%was significantly different treated with arsenic trioxide combining 3-methyl adenine ( 3-MA ) while it was ( 33.4 ±9.1 )% treated with arsenic trioxide alone, however, LC-3B protein expression gradually decreased.Jurkat cell apoptosis rate ( 44.96 ±3.60 )% was significantly increased treated with arsenic trioxide combining autophagy inhibitor(3-MA) while it was (2.94±0.26)% treated with arsenic trioxide alone, and this difference was statistically significant.Conclusion: 3-MA increased apoptosis rates of Jurkat cells inducing by Arsenic trioxide and it may be related with inhibition of autophagy and induction of apoptosis.

Objective: To study the method of setting up,practicability and the cardiovascular physiological mechanism of slippery pulse model caused by alcohol.Methods: ①Scanned the heart and radial artery(cunkou) by the Magnetom Avanto 1.5T and record the pressure wave of radial artery by NX-6 Pulse Diagnosis Equipment after the subjects had gotten 100ml of distillate spirit of 61 degree and defined as the slippery pulse.②Record the pressure wave of carotid artery of rabbits defined as the slippery pulse after intragastric administration of distillate spirit of 40 degree 8ml per kilogram by NX-3 Pulse Diagnosis Equipment.Results: ①Slippery pulse model of human subjects: the stroke volume(SV),cardiac output(CO),cardiac index(CI),ejection fraction(EF) and the peak velocity of the blood flow increased;the end-diastole volume(EDV) and end-systole(ESV) decreased;the periph blood vessel distended and the amplitude of the offset of the axis stepped up;the characteristics of the pulse wave of cunkou were consistent with the characteristics of typical slippery pulse wave.②The characteristics of the pulse wave of slippery pulse model of rabbits were coincident with that of human subjects.Conclusion: The method of setting up slippery pulse model with alcohol is consistent with the mechanism of slippery pulse in the theory of traditional Chinese medicine and is practicable.

Pulse shape and pulse force are difficult to detect in pulse taking study. But the application of visualized technology extends the space acquisition of pulse taking information, and it is possible to realize the objective detection of pulse shape and pulse force. Rational research thoughts and strategies could be informed by combining image information and other data, and it is a necessary method in implementing the objective detection of pulse.

Objective: To observe the relations between the parameters of pulse graph of pregnant and pathological slippery pulse and the indexes of hemorheology.Methods: The pusle diagram parameters(MSAB,MSBC,HFF',HE/HB and TW) of left guan pulse were extracted by the best extraction method,and the indexes of hemorheology(low shear ?b,high shear ?b,?p,Lb and Hct) were tested.Results: Compared with the control group,MSBC and HFF'of the two slippery pulse groups increased,but HE/HB and TW decreased significantly(P

Objective To study the role of endothelial cells on the inflammatory cytokine release in septic shock through the septic shock serum stimulating human primary endothelial cells (HPAEC) and peripheral blood mononuclear cells(PBMC).Methods PBMC isolated from healthy people by density gradient centrifugation.HPAEC cell surface markers CD144 and von Willebrand factor(vWF) molecule expression by RT-PCR and Western blot.Serum levels of IL-6,TNF-α,MCP-1 from septic shock patients and healthy human detected by ELISA.HPAEC and PBMC were stimulated with the isolated serums and LPS,respectively.ELISA was used to detect the supernatant IL-6,TNF-α,MCP-1 levels.HPAEC membrane molecules ICAM-1 expression was detected by flow cytometry with serum shock and LPS stimulation.Supernatant levels of IL-6,TNF-α,MCP-1 of HPAEC with S1P1 receptor agonist CYM-5442 pretreatment was detected by ELISA after shock serum stimulation.Results Endothelial cell markers CD144 and vWF molecules could be detected in the HPAEC.Levels of inflammatory cytokines IL-6,TNF-α,MCP-1 in patients with septic shock serum were significantly higher than healthy people (P＜0.01).PBMC and HPAEC with LPS or shock serum treatment respectively,compared with normal group,levels of inflammatory cytokines in the culture supernatant were significantly higher(P＜0.01).For PBMC,the level of inflammatory cytokines between shock group and LPS group were not significantly different (P＞0.05).But for HPAEC,levels of inflammatory cytokines in the supernatant of the shock group compared to the LPS group was significantly higher (P＜0.01).Similarly,when two cells after LPS stimulation,IL-6,TNF-α levels of HPAEC's supernatant were significantly lower than PBMC' s (P＜0.01),MCP-1 levels was no difference (P＞ 0.05).But when the stimulation of shock serum,HPAEC of IL-6,TNF-α levels and PBMC no significant difference (P ＞0.05).MCP-1 was significantly increased (P＜0.01).Shock patients serum stimulation S1P1 receptorspecific agonist CYM-5442 pretreatment of HPAEC with pretreatment of S1P1 receptor specific agonist CYM-5442,the culture supernatant of inflammatory cytokines IL-6,TNF-α,MCP-1 levels were significantly lower (P＜0.01).Conclusion Endothelial cells may play a central role on the release of inflammatory cytokine during septic shock.

Objective:To study the relationship between different pulse forces and cyclical power(CP)of radial artery motion.Methods:39 patients’and 20 healthy persons’ ECG and pressure sphygmograms were collected synchronously with NX-1 sphygmograph.On the basis of automatic detecting R waves of ECG and FFT analysis of pressure sphygmograms with SPTool of MATLAB 7.0.1,change in frequency domain of radial artery motion with different forces was observed and CP was worked out.Results: There is signifi cant deviation between CP of pulse tracings with different forces(pulse tracings with powerful force〉pulse tracings with normal force〉pulse tracings with weak force).Conclusion: CP of radial artery motion can be used to discriminate pulse force.

Pulse diagnosis system of traditional Chinese medicine is a complicated nonlinear macrosystem.Directed by complexity science theory,and introduced with idea of complex-simple-complex,the pulse diagnosis message is multidimensionally extracted,analyzed with reduced integration,and integrated with upgrade stage and dimension,according to four kinds of attributes "the Position,the Rate,the Shape,the Force".After the digitization,the new kind of pulses diagnosis instrument is manufactured with this mechanism.

Objective To explore the expression of early growth response gene-1 (EGR-1) in T cells that were positive for Tax protein of human T-cell leukemia virus type 1 (HTLV-1) and its possible reg-ulatory mechanism .Methods A series of expression structures carrying the regulatory elements of EGR-1 in different length and luciferase reporter genes were constructed .TaxP cells were transfected with the con-structs containing reporter genes and cultured with 5μmol/L of NF-κB inhibitor BAY 11-7082 or equal vol-ume of DMSO.After cultured for 24 hours the cells were collected to test the luciferase activity .BAY 11-7082 or equal volume of DMSO was added into the supernatant of TaxP cell culture to test the expression of EGR-1 protein by Western blot after 24 hours of culture .Tax and its mutants M22 and M47 were transfected into 293 T cells respectively to test the expression of EGR-1 protein by Western blot after 24 hours of culture . Results The expression structures carrying the regulatory elements of EGR-1 in different length and their mutants followed by luciferase reporter genes were successfully constructed .The luciferase activity in the cells transfected with the constructs containing the elements E 1 and E2 were higher than that transfected with E3, DelE and MutE, but the reporter gene expressions were decreased with the interference of BAY 11-7082 (P<0.01).However, there were no significant changes with the luciferase activity in the cells transfected by elements E3, DelE and MutE.Western blot analysis indicated that the expression of EGR-1 protein was significantly decreased with the interference of BAY 10-7082 .The expression of EGR-1 protein in M22 mu-tants-transfected 293 T cells were decreased significantly in comparison with those by wild type tax-and M47-transfected cells .Conclusion NF-κB was the key nuclear factor in regulating the expression of EGR-1 pro-tein in Tax-positive T cells .

The professional care by multi-disciplinary team and priority of prevention should be carried out in the treatment of diabetic foot disease to reduce diabetic amputation.This article describes the professional experience in the treatment of four complicated cases with diabetic foot disease and emphasizes the importance of the co-operation among different specialists,including diabetologists and wound,vascular,orthopedic surgeons,etc.as well as of varied therapies applied in staged management of the diabetic foot care,by treating these patients with diabetic foot disease as early as possible.