ObjectiveTo investigate the early diagnosis and treatment of pleuropulmonary blastoma in children. Meth-ods The progress of pleuropulmonary blastoma from type 1 to type 3 was retrospectively analyzed.Results The male infant had no obvious abnormality in chest X-ray at 18 days. At one year old, X-ray iflm of the chest showed cystic lesions near hilum of the left lung (about 5 cm × 4 cm × 4 cm). The congenital cystic adenomatoid malformation was considered and an operation resection was suggested. However, it had been rejected by his parents. At 3 years and 2 months old, chest X-ray and CT showed left lung had a solid cystic lesion, the volume was signiifcantly enlarged, the back side was solid and the upper part had a large gas cavity. Two months later, the patient had asthmatic suffocation. The chest CT showed the tumor was completely solid. Pleuropulmonary blastoma type 3 was considered. The patient received the conventional chemotherapy after operation excision, and was followed up for 1 year. No recurrence was observed after the treatment.Conclusions Type 1 pleuropulmonary blastoma and congenital cystic adenomatoid malformation are hard to be differentially diagnosed. Early diagnosis and treatment of type 1 pleuropulmo-nary blastoma can improve the prognosis.

Rabbit antisera against mouse LDH-C_4 were generated by immunization of rabbits with highly purified mouse lactate dehydrogenase C_4. Immunofluorescent localization of LDH-C_4 on mouse and human sperms was performed by rabbit antibodies against mouse LDH-C_4 at 1:20 dilution of antisera. Sheep anti-rabbit IgGFITC conjugates at dilution of 1:10 was used as second antibody for the detection. Human sperms were obtained from fresh semen of fertile males. Mouse sperms were prepared from epididymis fluid of mature mice. Both human and mouse sperms were thoroughly washed by PBS buffer. Sections of 4 ?m mouse testes were made and dried on the slides for the immunohistochemical localization, ?-hydroxy-n-valeric acid was used as specific substrate in incubation medium for revealing the LDH-C_4 activity. Immunofluorescent studies demonstrated that LDH-C_4 can be found on the surface of mouse and human spermatozoa. In human spermatozoa, the strong fluorescence was seen on the neck and midpiece. On mouse sperm, the antibody binding was directed toward the tail segment with little or no binding on the head or midpiece. Histochemical studies showed that the intensive formazen deposits were on the neck and midpiece of human and mouse spermatozoa, as well as the cells approximate to the lumen of mouse seminiferous tubules.

Objective To observe the effect of Modified Zhenxuan Granules (MZG) on blood pressure, synthesis and bioavail-ability of nitric oxide (NO), and plasma angiotensin I1 (Ang II) contents of spontaneously hypertensive rats (SHR). Methods Fiftymale SHR were randomly assigned to 5 groups: high-, middle-and low-dose MZG groups(at the doses of 37.50, 18. 70,and 9. 35 g/kg respectively), captopril (25 mg/kg) group and SHR control group. The rats received gastric gavage of corre-sponding medicine according to the experimental design for 12 weeks. Blood pressure from the tail was recorded at the beginning oftreatment and every 4 weeks during 12-weeks treatment. At the end of the experiment, the plasma NO and Ang II concentration,and aortal nitric oxide synthase (NOS) activity and aortal cyclic guanylic acid (cGMP) concentration were measured. ResultsMZG could decrease blood pressure and plasma Ang I1 concentration, increase plasma NO content, and enhance aortal NOS activ-ity and cGMP concentration ofSHR (P <0. 05, compared with the SHR control group), and high-dose MZG had the best effect.After treatment for 12 weeks, high-dose MZG had a similar effect on decreasing blood pressure compared with captoril group,had a better effect on increasing aortal cGMP (P< 0. 05), and increased the bioavailability of NO. Conclusion MZG can de-crease blood pressure, and its mechanism may be related to the improvement of NO/NOS/eGMP system and the decrease of plas-ma Ang Ⅱ concentration in SHR.

OBJECTIVE:To gain a clear idea about the situation of marking the period of validity on the wrapper and specifications of patent Chinese materia medica(PCMM) in China METHODS:To investigate the period of validity marking on wrapper and internal packing of 369 commonly-used PCMM in our hospital RESULTS:The PCMM with period of validity marking accounted for 65 9% of total PCMM(usually 1 5～3 years) and those without period of validity accounted for 34 1% CONCLUSION: The absence of period of validity marking is very common,we should pay attention to it

Objective: To apply fuzzy clustering analysis to determination of 10 inorganic elements and Cu/Zn ratio in 13 kinds of traditional Chinese medicine cured for vascular disease. Methods: By FAAS (flame atomic absorption spectrometry) sodium, calcium, magnesium, potassium, iron, chromium, nickel, manganese, copper and zinc were determined. By range analysis, correlation coefficients and relative fuzzy matrix were calculated.Results: Correlation coeffieients ? were mostly in the range of 0.55～0.80, especially ?=0.95 between Buzhangnaoxintong Capsule and Xinbao Pill. Conclusion: On condition that trace and macro elements are relatively rich and clustering correlation coefficients are larger, therapentic effects among traditional Chinese medicines cured for vascular disease are also similar. This suggests that trace elements, macro elements and Cu/Zn ratio have much to do with therapeutic effect.

Quality indicator is defined as the measure used to access the degree of inherent characteristics meeting the requirements .It is a powerful tool to improve laboratory quality to monitor and evaluate performance throughout critical steps in the total testing process .Targeted quality improvement can be obtained by quantizing quality levels in each phase when the quality indicators applied .Establishing and monitoring the quality indicators enables laboratory to compare over time between providers , and evaluate the effectiveness of delivered services and improving patient safety .

Objective To compare the diagnostic efficacy of the V/Q scan and CT pulmonary angiography (CTPA) for the detection of acute pulmonary embolism (PE) in elderly patients.Methods Fortyfour patients (age ≥60 years old) with suspected acute PE underwent V/Q scan and CTPA.The diagnosis of PE by V/Q scan was based on the criteria of prospective investigation of PE diagnosis (PIOPED) Ⅱ and the prospective investigative study of acute PE diagnosis (PISA-PED).The final diagnosis was made clinically.The sensitivities,specificities and accuracies of PIOPED Ⅱ,PISA-PED,CTPA and Wills score were calculated and compared using x2 and Fisher's exact tests.Kappa analysis was used to analyze the diagnostic accordance rate of PIOPED Ⅱ and PISA-PED.Results The sensitivities of PIOPED Ⅱ,PISA-PED and CTPA in the diagnosis of PE were 70.00％ (14/20),84.62％ (22/26) and 65.22％ (15/23),respectively (x2 =0.069-1.545,all P＞0.05).The sensitivity of Wills score was significantly lower (23.08％,3/13).The specificity of CTPA (93.75％,15/16) was significantly higher than those of PIOPED lⅡ and PISAPED (80.00％,12/15 and 61.11％,11/18,both P＜0.05).The accuracies of PIOPED Ⅱ,PISA-PED and CTPA were 74.29％ (26/35),75.00％ (33/44) and 76.92％ (30/39),respectively (x2 =0.005-0.070,all P＞0.05).The accuracy of Wills score was significantly lower (52.17％,12/23).The diagnostic accordance rate of PIOPED Ⅱ and PISA-PED criteria was 77.14％(27/35),Kappa=0.547,P＜0.05.Conclusion V/Q scan and CTPA have no significant difference for the diagnosis of PE in the elderly patients.

Objective To evaluate whether second-look endoscopy could prevent endoscopic submucosal dissection complicated with delayed bleeding and to clarify what kind of lesions that need second-look endoscopy. Methods 98 patients with early gastric cancer by histological diagnosis from Oct. 2014 to Sep. 2016 were included in this study. Mucosal damage related bleeding within 24 h after ESD was considered delayed bleeding. Clinical data of patients, lesions, surgical factors, and the incidences of bleeding before and after the second endoscopy examination were retrospectively studied. Results The en bloc resection rate of 98 patients was 100.0%, and all incisal margins were negative. No gastrointestinal perforation, death or severe complications occurred. 5.1%of the patients (5/98) occurred delayed bleeding after ESD and had been successfully stopped in a second-look endoscopy, and none received operations. No delayed bleeding occurred in follow-ups of patients with negative delayed bleeding. 40.0 % of the patients (2/5) with delayed bleeding received blood transfusion. The median time of second-look endoscopy after ESD was the second day after operation (1 ~ 3 d). The median bleeding time of 5 patients with delayed bleeding after ESD was the first day after operation (1 ~ 10 d). The median duration of surgery was 75 min (60~150 min), and the prediction success rate was 94.9 %. The results of univariate analysis showed that age [(69.6 ± 7.9) vs (60.9 ± 10.1) years old, P = 0.003] and operation time [(90.0 ± 41.0) vs (66.0 ± 42.0) min, P = 0.000] were both the risk factors in delayed bleeding and non-bleeding group. Binary Logistic regression analysis showed that operation time (OR^ =1.07; 95%CI:0.73 ~ 14.63, P = 0.010) was the only predictor of delayed bleeding after ESD. Conclusions Second-look endoscopy might be effective in preventing delayed bleeding after gastric ESD, especially within 48 hours after ESD. The operation time was an independent risk factor for delayed bleeding after gastric ESD.

Objective To establish a method for the determination of the antifungal component produced by Streptomyces NG-715.Methods The antifungal component was used as the reference component for UV spectrophotometry detemined at 339 nm. Results By spectrophotometry,a good linearity was established within 8μg/mL~20 μg/mL,and the average recovery rate was 99.98%(n=6 )with RSD being 0.1 1%.Conclusion The method is simple,rapid,accurate with good reproducibility for determining the antifungal component produced by Streptomyces NG-715.

ObjectiveTo study the effect of heat stress on the cytoskeleton and cell cycle of human umbilical vein endothelial cell (HUVEC) in vitro.Methods HUVEC was cultured in vitro in 5%CO2 medium at 37℃ (control group) or 43℃ (heat stress group) for 1 hour. Coomassie brilliant blue R-250 staining was used to determine the effect of heat stress on the cytoskeleton. The cells in heat stress group were subsequently cultured at 37℃in 5%CO2 medium after heat stress for 1 hour, and cell cycle of HUVEC was determined at 0, 6, 12, 18 and 24 hours with flow cytometry.Results Under light microscopy normal cytoskeleton was observed in control group, but thicker and shorter cytoskeleton was found after a rise of temperature, and stress fibers were found in heat stress group. The DNA content of HUVEC at all time points in G0/G1 stage was 38.07%-55.19% after heat stress. The DNA content in control group was 48.57%, and it was 54.06%, 55.19%, 48.23%, 38.07%, and 41.03% at 0, 6, 12, 18, 24 hours in G0/G1 stage in heat stress group. DNA content in S phase was 35.33%-48.18%. The DNA content in control group was 44.62%, and it was 35.33%, 39.50%, 42.50%, 48.18%, and 47.99% at 0, 6, 12, 18, 24 hours in S stage in heat stress group. DNA content in G2/M phase was 5.31%-13.75%. The DNA content in control group was 6.81, and it was 10.61%, 5.31%, 9.27%,13.75%, and 10.98% at 0, 6, 12, 18, 24 hours in G2/M stage in heat stress group. It was demonstrated that compared with control group, the DNA content in G0/G1 stage was significantly increased when the HUVEC were separated from heat stress within 6 hours, and it recovered at a similar level as control group at 12 hours.Conclusion Heat stress can change the cytoskeleton of HUVEC, and cause stagnation at G0/G1 stage in cell cycle.