ObjectiveTo investigate the early diagnosis and treatment of pleuropulmonary blastoma in children. Meth-ods The progress of pleuropulmonary blastoma from type 1 to type 3 was retrospectively analyzed.Results The male infant had no obvious abnormality in chest X-ray at 18 days. At one year old, X-ray iflm of the chest showed cystic lesions near hilum of the left lung (about 5 cm × 4 cm × 4 cm). The congenital cystic adenomatoid malformation was considered and an operation resection was suggested. However, it had been rejected by his parents. At 3 years and 2 months old, chest X-ray and CT showed left lung had a solid cystic lesion, the volume was signiifcantly enlarged, the back side was solid and the upper part had a large gas cavity. Two months later, the patient had asthmatic suffocation. The chest CT showed the tumor was completely solid. Pleuropulmonary blastoma type 3 was considered. The patient received the conventional chemotherapy after operation excision, and was followed up for 1 year. No recurrence was observed after the treatment.Conclusions Type 1 pleuropulmonary blastoma and congenital cystic adenomatoid malformation are hard to be differentially diagnosed. Early diagnosis and treatment of type 1 pleuropulmo-nary blastoma can improve the prognosis.

Rabbit antisera against mouse LDH-C_4 were generated by immunization of rabbits with highly purified mouse lactate dehydrogenase C_4. Immunofluorescent localization of LDH-C_4 on mouse and human sperms was performed by rabbit antibodies against mouse LDH-C_4 at 1:20 dilution of antisera. Sheep anti-rabbit IgGFITC conjugates at dilution of 1:10 was used as second antibody for the detection. Human sperms were obtained from fresh semen of fertile males. Mouse sperms were prepared from epididymis fluid of mature mice. Both human and mouse sperms were thoroughly washed by PBS buffer. Sections of 4 ?m mouse testes were made and dried on the slides for the immunohistochemical localization, ?-hydroxy-n-valeric acid was used as specific substrate in incubation medium for revealing the LDH-C_4 activity. Immunofluorescent studies demonstrated that LDH-C_4 can be found on the surface of mouse and human spermatozoa. In human spermatozoa, the strong fluorescence was seen on the neck and midpiece. On mouse sperm, the antibody binding was directed toward the tail segment with little or no binding on the head or midpiece. Histochemical studies showed that the intensive formazen deposits were on the neck and midpiece of human and mouse spermatozoa, as well as the cells approximate to the lumen of mouse seminiferous tubules.

Objective To observe the effect of Modified Zhenxuan Granules (MZG) on blood pressure, synthesis and bioavail-ability of nitric oxide (NO), and plasma angiotensin I1 (Ang II) contents of spontaneously hypertensive rats (SHR). Methods Fiftymale SHR were randomly assigned to 5 groups: high-, middle-and low-dose MZG groups(at the doses of 37.50, 18. 70,and 9. 35 g/kg respectively), captopril (25 mg/kg) group and SHR control group. The rats received gastric gavage of corre-sponding medicine according to the experimental design for 12 weeks. Blood pressure from the tail was recorded at the beginning oftreatment and every 4 weeks during 12-weeks treatment. At the end of the experiment, the plasma NO and Ang II concentration,and aortal nitric oxide synthase (NOS) activity and aortal cyclic guanylic acid (cGMP) concentration were measured. ResultsMZG could decrease blood pressure and plasma Ang I1 concentration, increase plasma NO content, and enhance aortal NOS activ-ity and cGMP concentration ofSHR (P <0. 05, compared with the SHR control group), and high-dose MZG had the best effect.After treatment for 12 weeks, high-dose MZG had a similar effect on decreasing blood pressure compared with captoril group,had a better effect on increasing aortal cGMP (P< 0. 05), and increased the bioavailability of NO. Conclusion MZG can de-crease blood pressure, and its mechanism may be related to the improvement of NO/NOS/eGMP system and the decrease of plas-ma Ang Ⅱ concentration in SHR.

OBJECTIVE:To gain a clear idea about the situation of marking the period of validity on the wrapper and specifications of patent Chinese materia medica(PCMM) in China METHODS:To investigate the period of validity marking on wrapper and internal packing of 369 commonly-used PCMM in our hospital RESULTS:The PCMM with period of validity marking accounted for 65 9% of total PCMM(usually 1 5～3 years) and those without period of validity accounted for 34 1% CONCLUSION: The absence of period of validity marking is very common,we should pay attention to it

BACKGROUND:Cone-beam computed tomography has been widely applied in the dentistry due to irreplaceable advantages. Among those advantages, linear measurement is one of the important ones. Further studies are suggest to explore the accuracy and repeatability of linear measurement for the whole skul , as wel as the effect of different resolution and scanning thickness on the linear measurement results.
OBJECTIVE:To evaluate the accuracy of linear measurement using cone-beam computed tomography (Newtom VG) on the human skul , with two different voxel sizes.
METHODS:In this study, 22 anatomic landmarks in four dry human skul s were marked and 11 linear measurements were obtained. These were considered to be the gold standard (real measurement). The skul s were scanned by cone-beam computed tomography (Newtom VG) at two voxel sizes:0.3 mm and 0.15 mm. Linear measurement was performed in axial and coronal planes. SPSS software version 17 was used for data analysis between radiographic measurement and real measurement.
RESULTS AND CONCLUSION:The mean differences of real and radiographic measurements were-0.27 to 0.14 in four different sections, and statistical analysis showed no significant difference between linear measurements and gold standard (P>0.05). There was no significant between two different voxel sizes (P>0.05). Cone-beam computed tomography (Newtom VG) is highly accurate and reproducible in linear measurements in the axial and coronal planes. A cone-beam computed tomography scan with a larger voxel size (0.3 mm) is recommended, resulting in lower radiation dose and faster scan time.

Objective To compare the diagnostic efficacy of the V/Q scan and CT pulmonary angiography (CTPA) for the detection of acute pulmonary embolism (PE) in elderly patients.Methods Fortyfour patients (age ≥60 years old) with suspected acute PE underwent V/Q scan and CTPA.The diagnosis of PE by V/Q scan was based on the criteria of prospective investigation of PE diagnosis (PIOPED) Ⅱ and the prospective investigative study of acute PE diagnosis (PISA-PED).The final diagnosis was made clinically.The sensitivities,specificities and accuracies of PIOPED Ⅱ,PISA-PED,CTPA and Wills score were calculated and compared using x2 and Fisher's exact tests.Kappa analysis was used to analyze the diagnostic accordance rate of PIOPED Ⅱ and PISA-PED.Results The sensitivities of PIOPED Ⅱ,PISA-PED and CTPA in the diagnosis of PE were 70.00％ (14/20),84.62％ (22/26) and 65.22％ (15/23),respectively (x2 =0.069-1.545,all P＞0.05).The sensitivity of Wills score was significantly lower (23.08％,3/13).The specificity of CTPA (93.75％,15/16) was significantly higher than those of PIOPED lⅡ and PISAPED (80.00％,12/15 and 61.11％,11/18,both P＜0.05).The accuracies of PIOPED Ⅱ,PISA-PED and CTPA were 74.29％ (26/35),75.00％ (33/44) and 76.92％ (30/39),respectively (x2 =0.005-0.070,all P＞0.05).The accuracy of Wills score was significantly lower (52.17％,12/23).The diagnostic accordance rate of PIOPED Ⅱ and PISA-PED criteria was 77.14％(27/35),Kappa=0.547,P＜0.05.Conclusion V/Q scan and CTPA have no significant difference for the diagnosis of PE in the elderly patients.

To improve the students'! comprehensive quality and cultivate their innovative medical talents, we applied different forms of discussion teaching in different stages of the students. In the course of theory, the form of short discussion was adopted to improve the students'! interest and concentration. In preclinical practice stage, the method of problem centered discussion was used to consolidate the profes-sional knowledge of the students. Discussion in the clinical practice was based on the actual problem of diagnosis and treatment, in order to cultivate the students'!clinical thinking. Through the diversified practice of discussion teaching with positive teaching evaluation, students'!interest in oral and maxillofacial surgery and the quality of teaching were improved.

Objective To investigate the inhibitory effect of earthworm fibrinolytic enzyme (EFE) on the metastasis of human hepatoma cells.Methods A metastatic model of human hepatocellular carcinoma in nude mice via orthotopic implantation was established.After the modeling for 7 days,the mice were divided randomly into the control group,low-dose EFE group (800 uku/kg?d-1),and high-dose EFE group (1600 uku/kg?d-1).After administration for 30 days,the implanted tumors were weighted,and the intrahepatic transmission rate and abdominal cavity seeding rate were calculated after examination by naked eyes.Then the pulmonary metastasis were examined under microscope,and the expression of focus adhesion kinase (FAK) and ?1-intigrin were detected through RT-PCR and western blotting method.Results Compared with the model control group,the mean weight of the orthotopic tumor was reduced,and the intrahepatic transmission rate and abdominal cavity seeding rate were decreased in FEF groups (P

Objective To evaluate whether second-look endoscopy could prevent endoscopic submucosal dissection complicated with delayed bleeding and to clarify what kind of lesions that need second-look endoscopy. Methods 98 patients with early gastric cancer by histological diagnosis from Oct. 2014 to Sep. 2016 were included in this study. Mucosal damage related bleeding within 24 h after ESD was considered delayed bleeding. Clinical data of patients, lesions, surgical factors, and the incidences of bleeding before and after the second endoscopy examination were retrospectively studied. Results The en bloc resection rate of 98 patients was 100.0%, and all incisal margins were negative. No gastrointestinal perforation, death or severe complications occurred. 5.1%of the patients (5/98) occurred delayed bleeding after ESD and had been successfully stopped in a second-look endoscopy, and none received operations. No delayed bleeding occurred in follow-ups of patients with negative delayed bleeding. 40.0 % of the patients (2/5) with delayed bleeding received blood transfusion. The median time of second-look endoscopy after ESD was the second day after operation (1 ~ 3 d). The median bleeding time of 5 patients with delayed bleeding after ESD was the first day after operation (1 ~ 10 d). The median duration of surgery was 75 min (60~150 min), and the prediction success rate was 94.9 %. The results of univariate analysis showed that age [(69.6 ± 7.9) vs (60.9 ± 10.1) years old, P = 0.003] and operation time [(90.0 ± 41.0) vs (66.0 ± 42.0) min, P = 0.000] were both the risk factors in delayed bleeding and non-bleeding group. Binary Logistic regression analysis showed that operation time (OR^ =1.07; 95%CI:0.73 ~ 14.63, P = 0.010) was the only predictor of delayed bleeding after ESD. Conclusions Second-look endoscopy might be effective in preventing delayed bleeding after gastric ESD, especially within 48 hours after ESD. The operation time was an independent risk factor for delayed bleeding after gastric ESD.