Marine bacteria from the samples of sea sediments and seawater were directly plated on isolation media and the biodiversity of isolates was examined with DNA fingerprinting.542 single colonies were obtained from the media.ARDRA with enzyme Hinf I revealed 16 operational taxonomic units(OTU) which were dominated by OTU5 group which accounts for 19 isolates,and OTU7 group which accounts for 11 isolates.The biodiversity of isolates from these two dominant OTU groups was further investigated by a genomic fingerprinting technique, ERIC-PCR.The results indicated that there were 12 different ERIC-PCR types present among the OTU5 group while only 4 among the OTU7.The data indicated rich diversity profiles of marine microorganisms were presented in the East China Sea.

Objective To establish the determination of Dehydroandrographolide in Ganmaoqing Capsule. MethodsThe determination was carried out by HPLC with a KromasilC-18 column(250 mm ×4.6 mm,5μm),methanol-water(65:35)severed as the mobile phase,the speed was 1mL/min and the detection wavelength was at 254 nm. ResultsDehydroandrographolide showed a good linear relationship at the range of 0.04872 μg ～0.38976 μg(r2 =0.9995,n= 8);The average recovery of Dehydroandrographolide was 100.1%(RSD= 2.92%). ConclusionHPLC method was sensitive,accurate,reproducible,specific and could be used for quality control of Ganmaoqing Capsule efficiently.

China ; Humans ; Industry ; Pneumoconiosis

Objective To detect the expression of MSI2 protein and mRNA in pancreatic ductal adenocarcinoma (PDAC) tissue,and investigate the correlation between the expression of MSI2 protein and the clinicopathological parameters.Methods The expression of MSI2 protein in 61 PDAC specimens and paired adjacent non-cancerous pancreatic tissues were detected by immunohistochemistry.Western blot and quantitative real-time PCR (QRT-PCR) were used to examine the expression of MSI2 protein and mRNA level in 10 PDAC specimens and adjacent non-cancerous pancreatic tissues.Then the relationship between MSI2 expression in cancerous tissues and clinicopathological parameters was analyzed.Results In 61 patients with PDAC,the expression rate of MSI2 protein was higher in cancerous tissues (63.9％) compared with that in paired non-cancerous pancreatic tissues (41.0％),and the difference between the two groups was statistically significant (t =2.809,P =0.007).The expression levels of MSI2 protein in 10 fresh PDAC specimens and adjacent non-cancerous pancreatic tissues were 0.748 ± 0.195 and 0.420 ± 0.171,and the expression level of MSI2 mRNA in PDAC specimens was as 2.507 ± 2.981 times as much of adjacent non-cancerous pancreatic tissues,and the difference between the two groups was statistically significant (t =3.689,P=0.005;t =2.660,P =0.026).The expression of MSI2 in cancerous tissues was only positively associated with the size of the tumor (x2 =5.096,P =0.024),but it was not associated with other parameters.The median survival of patients with high MSI2 expression was 321 d,and it was 730 d for patients with low MSI2 expression,and the median survival of patients with high MSI2 expression was significantly shorter than that of low MSI2 expression (x2 =6.706,P =0.010).Conclusions The expression MSI2 is up-regulated in PDAC and related to the tumor size.The patients with high expression of MSI2 protein have poor prognosis.

To investigate the effects of salvianolic acid B (SA-B) on extracellular signal-regulated kinase (ERK) signal transduction pathway activated by transforming growth factor-β1 (TGF-β1) in rat hepatic stellate cells (HSCs).

Objective To explore the relationship between learning and memory ability and antioxidant enzyme (SOD, CAT, GSH-Px)activities in specific brain areas of D-galactose model rats. Methods The aging models were induced by D-galactose in 3-month old rats, and the learning and memory ability of aging model was detected in model MG-2 maze. The activity of SOD, CAT and GSH-Px in brain cortex, cerebellum,hippocampus, striatum and hypothalamus were detected immediately after rats were executed. Results The learning and memory ability of D-ga-lactose aging model was lower than that of control group. The difference of antioxidant enzyme activity among different rat brain areas was significant. The correct response rate of D-galactose aging model was( 58.9 ± 5.4 ) %, which is significantly lower than that of the control group (66.8 ±8.9) %, P ＜ 0.05. The times of response required reaching the standard after 24h of aging model rats was significantly higher than that of the control( Aging group :29.5 ± 12.8, Control group 16.6 ± 6.2; P＜ 0.01 ). Correct response rate after 24h was significantly lower than that of the control ( Aging group :67.3 ± 10.3 , Control group: 79.1 ± 6.9; P＜ 0.01 ). The SOD activity in cortex, hippocampus and striatum( Aging group :66.12 ±5.89,80.46 ± 6.57,87.82 ± 6.87; Control group :57.63 ± 4.21,67.46 ± 6.80,68.37 ± 6.52) significantly reduced ( P ＜ 0.05 and P ＜ 0.01 ).The CAT activity in cortex, hippocampus, striatum and hypothalamus( Aging group :6.45 ± 0.55,5.86 ±0.35,6.93 ± 0.63,8.89 ± 0.38; Control group :5.32 ± 0.56,4.76 ± 0.38,4.37 ± 043,6.11 ± 0.37) significantly reduced ( P ＜ 0.05 and P ＜ 0.01 ). GSH-Px activity was similar in each brain area without significance ( P ＞ 0.05 ). Conclusions The learning and memory ability of aging mode rats decreased. The change of antioxidant enzyme activities in hippocampus, cortex, striatum and hypothalamus is closely related to learning and memory ability.

Objective To explore the effects of antisense NF-?B p65 oligodeoxynucleotides on UV-induced IL-6 secretion of keratinocytes. Methods NF-?B p65 oligodeoxynucleotides were transfected to a keratinocyte cell line, HaCaT cells, mediated by lipofectamine. The NF-?B p65 protein in the cells was measured by Western blot assay, the mRNA level of NF-?B p65 was detected by RT-PCR, and UV-induced IL-6 level was determined by ELISA pre- and post-transfection and/or UVB irradiation. Results The NF-?B p65 protein expression was significantly increased in UVB (10, 20, 30 mJ/cm2 ) irradiation groups as compared with that of the control groups (P

0.25)levels in the two groups.In the group treated with42mJ/cm 2 UVB irradiation followed by the addition of EGCG,the numbers of apoptotic and dead cells and Fas mRNA were decreased,but bcl-2protein was increased.Conclusions Low dosage of UVB irradiation could induce apoptosis of keratinocytes.High dosage of UVB irradiation might result in cell death.EGCG could reduce UVB-induced apoptosis of keratinocytes by increasing bcl-2protein and decreasing Fas mRNA.

Diverticulitis of colon,the inflammation of colonic diverticulars,is connected with dietary,age and disorder of colonic environment.Due to the different cultures between western contries and orient nations,the morbidity,predilection site,clinical manifestation and management are different in diverticulitis of colons.Computed tomography(CT)has been the key diagnosic method,and it is able to guide transfixion pins to treat diverticulitis of colon.After medical treatment,most of patients can be relaxed,and only someone need operations.

OBJECTIVE:To strengthen the inspection for qualification of new drugs when introduced in order to guarantee the quality of new drugs and the safety of drug use.METHODS:The problems existing in the inspection for qualification of new drugs were exemplified and classified.RESULTS&CONCLUSION:There existed the problems in the respect of validity,authenticity,effectiveness,and integrality.The good maintainance of the database of SFDA plays a important role in in-specting the new drugs when introduced,while on the other hand such issues as establishment and use of credibility file in medical agencies are worth discussing.