Objective To study the safety of Plumula nelumbinis microcapsules and its anti-arrhythmic effect.Methods Acute toxicity test in mice,arrhythmia mice models induced by chloroform and arrhythmia rats models induced by barium chloride(BaCl2)were adopted.Results The maximum tolerance dose for mice was 16.0 g/kg body weight.The microcapsule decreased the incidence of chloroform-induced ventricular fibrillation in mice significantly(P 0.05,compared with the blank control group).Conclusion The preliminary results show that Plumula nelumbinis microcapsule is safe,and exerts anti-arrhythmic effect against experimental arrhythmia.

Objective To retrospectively analyze of blood culture positive for adult patients with serum calcium concentration ,to understand the relationship between bloodstream infections in patients and serum calcium .Methods From January 2013 to July 2015 ,serum calcium (Ca) ,phosphorus (P) ,alkaline phosphatase (ALP) in 78 cases with the blood culture positive adult patients and 103 healthy subjects were detected and analyzed .Results The value of CA in adult patients was(1 .89 ± 0 .18) mmol/L ,healthy subjects while of that was (2 .23 ± 0 .23)mmol/L in healthy subjects ,There was statistically significant (P<0 .01) in two groups ;The P of patients was (1 .23 ± 0 .24)mmol/L ,which in healthy subjects was (1 .25 ± 0 .28)mmol/L ,There was no statistical differ‐ence (P>0 .05);ALP of patients was (78 ± 42)U/L ,which was (76 ± 40)U/L in healthy subjects .There was no statistical differ‐ence (P>0 .05) ,the r of Ca and P ,Ca and ALP were 0 .023 ,0 .023 respectively (P>0 .05) in patients ,while there were 0 .125 and 0 .132 (P<0 .05) in healthy subjects .Conclusion Calcium in serum of adult patients with blood culture positive might be reduced .

Objective To investigate the effect of FTY720 and gemcitabine on the proliferation and apoptosis of H520 and A549 cells in non-small cell lung cancer(NSCLC) cell line.Methods The interventional influence on the in vitro cultured NSCLC A549 and H520 cells was performed by selecting 0,2,4,6,8,10 μmol/L concentrations of FTY720,then the absorbance value was detected at 24,48,72 h after culture and the proliferation inhibiting effects of FTY720 on A549 and H520 were observed under the condition of different concentration of FTY720;adding single 7 μmol/L of FTY720,single 0.2 μmol/L gemcitabine and 37 μmol / L FTY720 combined with 0.2 mol/L gemcitabine into A549 and H520 cells lines,then the differences of inhibition and apoptosis after 48 h in the cells of each group were observed.Results The inhibitory effect of different concentrations of FTY270 on NSCLC A549 and H520 cell lines was statistically significant difference (P＜0.05).The proliferation inhibiting effect of FTY720 on NSCLC H520 and A549 cell lines had the correlation with the concentration and time.The apoptosis rate of FTY720 combined with gemcitabine on A549 and H520 cells was significantly higher than that of single use in these two drugs (P＜0.05).Conclusion FTY720 combined with gemcitabine can significantly inhibit the proliferation of A549 and H520 in human NSCLC,and can effectively promote the apoptosis of cancer cells,and has the higher clinical value.

Objective To explore the intervention effects of trichostatin A ( TSA ) on specialization of rat bone marrow mesenchymal stem cells ( MSCs ) .Methods The rat MSCs were isolated, cultured and purified by the whole bone marrow adherent method in vitro, with morphological observation.The third generation of MSCs were selected, directional induced to osteoblasts, and divided into the TSA low dose group (0.1μmol/L), middle dose group (1μmol/L) , the high dose group (10μmol/L) according to different drug concentrations, seting up blank control group at the same time.MSCs proliferation and cell growth curve of each group were drawn by MMT, the activity of alkaliphosphatase ( ALP) was detected, and the levels of corebinding factor α1 (Cbfα1), basic fibroblast growth factor (bFGF) and insulin-like growth factors-1 (IGF-1) mRNA were detected by RT-PCR. Results The trend of MSCs growth curves in each groups were similar, compared with control group, the growth curve of TSA low dose group had no significant change, the TSA middle dose and high dose significantly promoted the proliferation of MSCs (P<0.05).Compared with control group, ALP activity of TSA low-, middle-and high-dose group were significantly higher at 4th,5th,6th(P<0.05).The expression levels of Cbfα1, bFGF and IGF-1mRNA were significantly higher than those of control group, respectively (P<0.05).Conclusion TSA can significantly promote the rat bone marrow mesenchymal stem cells to differentiate into osteoblast, which is possibly associated with up-regulation of Cbfα1, bFGF and IGF-1mRNA level.

Objective To investigate the clinical significance between congestive heart failure (CHF) and serum thyroxine. Methods 50 patients with CHF (7 cases of grade Ⅱ heart function,grade Ⅲ 19 cases,grade Ⅳ 24)and 30 normal persons wre selected. The serum triiodothyronine( T3 ), four-triiodothyronine( T4 ), free triiodothyronine (FT3), free triiodothyronine (FT4), thyroid stimulating hormone TSH were determined by radioimmunoassay. Results The T3, FT3 contens ( 1.95 ± 0.55) nmol/L, (2.20 ± 0.39 ) pmol/L in CHF group were lower than the control group (2.4 ± 0.50) nmol/L, ( 5.89 ± 0. 45 ) pmol/L ( t = 2. 415,2. 325, P < 0.05 ); The T3, FT3 contens ( 2.19 ± 0. 53 )nmol/L,(3.69 ± 0.57)pmol/L after treatment were significantly higher than that before treatment (t =2. 375,2. 405, P < 0.05 ); The T3, FT3 contens in cardiac function with stage Ⅳ ( 1.76 ± 0.7) nmol/L, ( 1.99 ± 0. 45 ) pmol/L were significantly lower than the control group( t = 2.245,2. 375, P < 0.05 ); Serum TT3, TT4 and cardiac function was negatively correlated ( r = -0.933, -0.383, all P < 0.05 ) , serum FT3, FT4,TSH and cardiac function were not correlated. Conclusion The changes of serum thyroid level could be used as an index of the severity of heart failure.

Objective To explore the effects of a potential anti-oxidative plant,Erigeron multiradiatus(Asteraceae),plant materials from naturally distributed high-altitude populations at different stages of life cycle were collected.Methods Fifteen extracts obtained from the Ganzi region(Sichuan,China)were studied to assess their radical-scavenging ability on 1,1-diphenyl-2-picrylhydrazyl radicals and reducing power ability.Moreover,considering that anti-oxidants and free radical scavengers can also exert protective effect on endothelial cells from oxidative injury,these extracts were also evaluated for their anti-oxidative activity against cellular injury in the cultured human endothelial cell line(ECV304)induced by hydrogen peroxide(H<,2>O<,2>).Results All the extracts had radical-scavenging and/or reducing power ability,and the most active extract was found during flowering whereas the lowest appeared during vegetative growth period.The accumulation of anti-oxidative compounds was found to be affected by the altitude of growth environment.Total flavonoid content assay was also performed to support this outcome.Furthermore,these extracts also exhibited different effects on attenuating H<,2>O<,2>-induced cytotoxicity and inhibiting lipid peroxidation and LDH leakage from endothelial cells.Conclusion E.multiradiatus may be an important natural anti-oxidant and this property may contribute to verifying the utilization of this plant in Tibet folk medicine.

Objective Through the training,induction and observation,of biofilm Staphylococcus aureus(BF)in vitro to study fosfomycin(FOS)on the role of BF.Methods Select the clinical and quality control strains of Staphylococcus ATCC25922,ATCC25923 using trypsin broth medium(TSB-teflon)systems and saline(NS-teflon)system for the training,induction and confocal microscope observation of BF.The minimum inhibitory concentration(MIC)was determined by twice the agar dilution Drugs.Results After 1,3,7 d of incubation,clinical isolates of the biofilm accumulation over time of the extension increased After joining fosfomycin biofilm,the growth was inhibited.There was significant difference in MIC with or without biofilm(P

Objective To investigate the value of dual-energy virtual unenhanced and dual-energy iodine value distribution diagram type of kidney cancer.Methods A retrospective analysis of 35 cases of renal cell carcinoma dual-energy CT scan data,compared to kidney pathology data,analyze the image quality and radiation dose dual-energy virtual unenhanced and dual energy distribution of iodine in clear cell carcinoma and non-clear cell carcinoma the differences that exist.Results Unenhanced and virtual unenhanced image quality was quite,CT value (routine unenhanced renal parenchyma 31.90±4.10 HU,abdominal aortic 45.60±6.30 HU,psoas 50.00±6.6 HU3,kidney 1.410±17.06 HU; virtual unenhanced renal parenchyma abdominal aortic 33.20±5.50 HU,48.30±8.00 HU,52.40±10.63 HU psoas kidney 0.87±17.48 HU),signal to noise ratio (routine unenhanced renal abdominal aorta 3.22±1.49,5.13±2.57,4.56±2.12 psoas kidney 3.24±2.27 virtual unenhanced renal abdominal aorta 2.73±1.08,3.79±1.64,3.23±1.35 psoas kidney 2.18± 1.57) had not significantly different (P ＞ 0.05).Clear cell carcinoma of the skin medullart junction of iodinedistribution of images Iodine (2.75±1.05) mg/ml was higher than non-clear cell carcinoma (1.25±0.72) mg/ml,the difference was statistically significant (t =7.048,P ＜ 0.05).Conclusions CT examination in patients with renal cell carcinoma virtual unenhanced image quality is similar with routine unenhanced image quality,it reduces radiation dose.The distribution of iodine can provide more diagnostic information that can help us be more intuitive and accurate judgments of the blood supply and kidney cancer subtypes.

BACKGROUND:Closed reduction and interlocking intramedullary nailing can protect blood supply of fractured bone,decrease infection and promote bone healing.which is becoming the first choice for treating femoral shaft fracture However,it is difficult to conduct in closed reduction and keep stabilization of fractured bone.OBJECTIVE:To explore the curative effect of closed reduction and interlocking intramedullary nailing in treating femoral shaft fracture with assist of rubber tourniquet 360°elastic fixation METHODS:From May 2008 to November 2009,18 patients(14 males,4 females;aged 18-65 years)with closed femoral shaft fracture were treated at the Dongguan Hospital of Traditional Chinese Medicine.Body skin was protected by cloth,elastic rubber tourniquet was doubled and 360°wrapped around the fractured bone with the length of 10 cm from up and down.Supracondylar femur was reamed by Kernig-needle;fractured bone was dealt with countertraction and horizontal rotation.Another assistant compressed the fractured bone according to the displacement of fracture,which contributes to bone fracture reduetion and interlocking intramedullary nailing.RESULTS AND CONCLUSION:All 18 patients were followed up postoperatively,no case was found with skim necrosis and damage of nerve and blood vessel.16 cases were bone union with excellent 15 cases,fine 1 case;2 cases were still being followed up.Callus was firstly found 1 month after operation and was obviously found in 3e months postoperatively The result suggested that the treatment of closed reduction and interlocking intramedullary nailing with assist of rubber tourniquet360°elastic fixation is a simple and applicable approach.which reduces the interruption of blood supply for fractured bone and closed reduction gets good result,which reduces the chance of open reduction.

In view of the fact that medical inspection equipment sold in the domestic market is mainly imported from abroad and very expensive, we developed a full-automatic fluorescence analyzer in our center, presented in this paper. The present paper introduces the hardware architecture design of FPGA/DSP motion controlling card+PC+ STM32 embedded micro processing unit, software system based on C# multi thread, design and implementation of double-unit communication in detail. By simplifying the hardware structure, selecting hardware legitimately and adopting control system software to object-oriented technology, we have improved the precision and velocity of the control system significantly. Finally, the performance test showed that the control system could meet the needs of automated fluorescence analyzer on the functionality, performance and cost.
Automation, Laboratory ; Equipment Design ; Fluorescence ; Software