Objective To explore the changes of the total antioxigenic capacity (T-AOC) of the plasma and red blood cells (RBC) in the acute pulmonary edema rats induced by acute formaldehyde inhalation. Methods SD rats were randomly dived into the formaldehyde group and control group, then were exposed to formaldehyde and physical saline vapor respectively in a chamber for 2 hours, the actions and vital signs were observed. After treatment the rats were taken out the chamber, 5 hours later, the T-AOC of the red blood cells and plasma were measured and the body, lungs and heart were weighted, the lung /heart weight index and lungs/body weight index were calculated, the pathological examination was done. Results Compare with the control group, the lung weight, lung/body weight index, lung /heart weight index and the T-AOC of the plasma increased, the T-AOC of RBC and body weight decreased significantly(P

Objective To explore the application and value of three-dimensional reconstruction technology in diagnosis and surgical therapy of ground-glass opacity.Methods We analyzed 188 cases with GGO during October 2012 and October 2014 in Shanghai First People’s Hospital affiliated to Shanghai Jiao tong University School of Medicine respectively.Several relative parameters based on three-dimensional reconstruction were selected to differentiate GGO pathology .By ROC curve anal-ysis, optimal cut-off points were determined.Volume-doubling time(VDT) was calculated among 104 cases which had been following up at least for three months and compared among AAH, MIA and IAC.Results Cases’ mean age was 56.66 ± 9.673years(ranged from 27 to 82 years) with 67 males and 121 females.The diameter(P<0.001), total volume(TV)(P<0.001), the maximum CT attenuation(MAX)(P<0.001) and standard deviation of distribution of CT attenuation within the whole GGO(STD)(P=0.015) of malignant group were significant larger than those of benign ones except for average CT at-tenuation(AVG)(P=0.094).The AUC were just 0.64-0.80 when differentiated benign group from AIS, benign group from MIA and AAH from MIA in comparison to 0.70-0.95 when it comes to differentiating benign group and AAH from IAC .Ad-ditionally, the optimal cut-off points were 13.5-15.5 mm in diameter, 400-600 mm3 in TV, -110 -20 Hu in MAX and 120-160 in STD.The mean VDT was 865.00 ±111.33, 464.67 ±44.40 and 238.36 ±76.71 for AAH, MIA and IAC re-spectively(AAH/MIA P<0.001, MIA/IAC P=0.003).Conclusion The diameter, TV, MAX and STD of GGO based on three-dimensional reconstruction could discriminate GGO availably .Combined utilization of HRCT and three-dimensional re-construction has a valuable significance in diagnosis and treatment of GGO .

: Objective To develop a rehabilitation device and software game, and provide the system for evaluation and training of anterior cruciate ligament (ACL) reconstruction.MethodsThe development and clinical application of a biofeedback knee joint movement rehabilitation system for ACL reconstruction were very important.ResultsBIOKS readings reproduce Zebris 3D motion analysis system measurements reliably between 0° and 150° ( P>0.05). Significant knee joint movement training effect ( P<0.05 ) in JPS assessment. However, there were no differences ( P≥0.05) between SST and KAS assessments.ConclusionThe improvement of joint position sense and body centroid sway under single leg support conditions.

Objective To conduct a Meta-analysis to evaluate the efficacy and safety of interfer on-α for treating HBeAg-positive chronic hepatitis B in children.Methods PubMed and Chinese Biomedical Database were searched from the beginning to April 2006,and the references of eligible studies were manually screened.Randomized controlled trials published in the English and Chinese literature comparing interferon-α with non-antiviral interventions(placebo or no treatment)in children with chronic hepatitis B were eligible for inclusion.Studies were included if patients were treated for at least 3 months and followed up for at least 6 months after cessation of therapy.Two investigators independently assessed the quality and extracted the data.The methodological quality of trails was assessed by the Jadad-scale plus allocation concealment.Heterogeneity was examined by Chi-square test.Fixed effects model or random effects model was used to pool the data.Sensitivity analyses were used in the treatment course.Results Seven randomized controlled studies with a total of 360 child chronic hepatitis B virus carriers who were positive for hepatitis B surface antigen and hepatitis B e antigen werc identified.It was found by Meta-analysis that,compared with the control,at the end of therapy,interferon-α could significantly clear HBeAg[22.1%vs 6.7%,OR 3.56,95% CI(1.74, 7.28),P=0.000 5],HBV DNA[33.7% vs 12.6%,OR 3.50,95% CI(2.03,6.06),P＜0.01], HBsAg [6.5% vs 0.5%,OR 7.10,95% CI(1.52,33.12),P=0.01],and achieve HBeAg seroconversion [17.3% vs 2.9%,OR 5.62,95% CI(1.65,19.18),P=0.006],but was not more effective in HBsAg seroversion[2.0% vs 0,OR 3.55,95%CI(0.35,35.93),P=0.28]and alanine aminotransferase(ALT)normalization[24.2% vs 16.2%,OR 1.72,95% CI(0.84,3.52), P=0.14].Conclusions Interferon a may be efficacious in clearance of HBeAg,HBV DNA and HhsAg, and achievement of HBeAg seroversion.Little evidence is available on HBsAg seroversion and ALT normali zation.Rigorously designed large sample size randomized double blind clinical trials with large sample size are required to further confirm and support the conclusion.

Objective: To research and establish an analysis model for chemical drug standards to provide the reference for the standard exploration jobs. Methods:The standard variation was studied by comparing and analyzing every version standard for the same drug to look for the change rule. Results:The standard items of the drug had some change rules in indicator numbers, testing methods and limits according to the order of issue time. The standard development trends could be described by making specific indicators and drawing evolution figures of the items. Conclusion:By the standard analysis model, the same item of the same drug can be compared to provide the reference for data collecting of experts. Meanwhile, formulate standard can be calculated to provide the reference for the standard improvement without drug standards.

Objective:To evaluate the effect of icariin on TNF-αexpression in periodental tissue during root resorption induced by ropid palatal expansion in rats.Methods:24 male Wistar rats were divided randomly into 3 groups(n =8)as group A(none-expansion control group),B(expansion group)and C(icariin and expansion group).In group B and C,an initial force of 50 g was applied to the area between the right and left upper first molars of the rats for 2 weeks for palatal expansion.The rats of group C were given intragastric administration of icariin at 2.5 mg/(kg·d)while the rats of group A and B were given the same dose place-bo.The centered buccal-lingual root and peridentium tissue slices were generated from the upper first molars of the rats.HE stai-ning of the slices was performed.The expression of TNF-αprotein at the compression sites was detected by irnmunohistochemistry. Results:The A values of TNF-αof group A,B and C were 0.030 2 ±0.001 7,0.241 0 ±0.002 4 and 0.088 6 ±0.002 8 respec-tively(B vs A or C,P <0.05).Conclusion:Icariin may inhibit TNF-αexpression in periodental tissue during root resorption in-duced by rapid palatal expansion.

In view of the fact that medical inspection equipment sold in the domestic market is mainly imported from abroad and very expensive, we developed a full-automatic fluorescence analyzer in our center, presented in this paper. The present paper introduces the hardware architecture design of FPGA/DSP motion controlling card+PC+ STM32 embedded micro processing unit, software system based on C# multi thread, design and implementation of double-unit communication in detail. By simplifying the hardware structure, selecting hardware legitimately and adopting control system software to object-oriented technology, we have improved the precision and velocity of the control system significantly. Finally, the performance test showed that the control system could meet the needs of automated fluorescence analyzer on the functionality, performance and cost.
Automation, Laboratory ; Equipment Design ; Fluorescence ; Software

Objective To evaluate the effect of integrative therapy and influencing factors in patients with intractable constipation (IC). Methods Eighty-four consecutive IC patients were divided into non overlap and overlap groups. All patients received individualized integrative therapeutic regimen for 4 weeks. The symptom scores of constipation and total effective rates were evaluated. Influencing factor of therapeutic effect in IC was analyzed.Results (1) IC of overlap group and non overlap group accounted for 40.5% (34/84) and 59.5% (50/84), respectively. (2)The total effective rates were 50.0% and 78.0% in overlap and non-overlap group, respectively(P

Objective To study the effects of Compound Di Gui Capsule (CDGC) on the levels of glucose and lipid in diabetic rats.Methods Rats models with diabetes mellitus were induced by intraperitoneal injection of streptozotocin (50mg?kg-1).Then the diabetic rats were divided into different groups at random.CDGC groups were given CDGC in the dosages of 2.4,1.2,0.6 g?kg-1?d-1 respectively by gastric gavage for 90 d.Levels of whole blood glucose,glycosylated hemoglobin,total cholesterol (TC),triglyceride (TG),low-density lipoprotein cholesterol (LDL-C),high-density lipoprotein cholesterol (HDL-C),nitric oxide (NO),total antioxidation capacity (T-AOC) were determined.The insulin level and the pathological changes of pancreatic tissues were also observed.Results The glucose level of diabetic rat was decreased 2h after administration.After administration of CDGC for 90d,the glycosylated hemoglobin,TG,LDL-C and NO were markedly decreased,islet ?cells were protected and restored,and the insulin level and T-AOC were increased.Conclusion CDGC can regulate the metabolism of glucose and lipid in diabetic rats,and can protect and restore the pancreatic islet cells.