AIM: To establish the quality standard for Guishao Soft Capsules(Radix Paeoniae Alba,Radix Angelicae Sinensis,Rhizoma Chuanxiong, etc.) METHODS: Radix Paeoniae Alba,Rhizoma Atractylodis,Rhizoma Chuanxiong,Rhizoma Alismatis and Radix Angelicae Sinensis were identified by TLC.The contents of albiflorin and paeoniflorin in Guishao Soft Capsules were determined by HPLC.HPLC analytical method was carried out(using) a C_(18) column and a mixture containing 17 volume of acetonitrile and 83 volume of 0.05% acetic acid as the mobile phase.The detection wavelength was set at 230 nm. RESULTS: Spots obtained from the test solutions had the same color in reference solution and medical material in the same location,and the blank solution had no interfernece.The linear range of albiflorin was from 0.53 ?g to 1.06 ?g(r=0.999 8),the average recovery was(100.3%.) The linear range of paeoniflorin was from 0.29 ?g to 2.00 ?g(r=0.999 9),the average recovery was(99.49%.) CONCLUSION: In TLC,the spots are very clear and specific to identify the herbal medicine in Guishao Soft Capsules.It is simple,quick,high precise and accurate for HPLC to control the quality of Guishao Soft Capsules.

Objective To study the pharmacokinetics of aristolochic acid A in Radix Aristolochiae and the compound preparation of Guanxinsuhe Capsule in mice in vivo after single-dose oral administration and observe the difference of aristolochic acid A absorption and distribution. Methods Aristolochic acid A assay was performed by RP-HPLC on a Waters apparatus with a DiamonsilTM C18 column (250 mm × 4.6mm, 5 μm), a mobil phase: a mixture of methanol-water-acetic acid (72: 27 : 1), flow rate: 1.0 mL/min, detection wavelength: 315 nm, and column temperature: 20 ℃. Results Mice were given Radix Aristolochiae and Guanxinsuhe Capsule by ig at the same level of 2. 5 mg/kg of aristolochic acid A, respectively, which were suspended in 0. 3% CMC-Na solution. Plasma concentrations were determined by RPHPLC. After single-dose ig administration of Radix Aristolochiae or Guanxinsuhe Capsule to mice, the mean plasma concentration-time courses of aristolochic acid A obtained fitted the one-compartment model.The main pharmacokinetic parameters of aristolochic acid A in Radix Aristolochiae, t1/2ka, t1/2 ke, tmax,AUC, Cmax are 5. 103 min, 43. 63 min, 17.89 min, 80. 45 (μg · min)/mL, and 0. 916 8 μg/mL; the rela tive pharmacokinetic parameters in Guanxinsuhe Capsule are 5. 294 min, 43.50 min, 18. 32 min, 33.08(μg · min)/mL, and 0. 381 8 μg/mL. Conclusion The Cmax of aristolochic acid A in Guanxinsuhe Capsule is significantly less than that in Radix Aristolochiae, which indicates that the compound compability could decrease the absorption of aristolochiae acid A.

Objective:To observe the effect of tuina manipulations on blood pressure and its variability in hypertension patients. Methods:Forty hypertension patients were randomized into an observation group and a medication group, 20 cases in each group. The observation group was intervened by tuina manipulations of kidney-tonifying blood-circulating and collaterals- unblocking in addition to regular medication, while the medication group was by the same medication. The 24-hour blood pressure monitoring was performed before intervention and after 3-month intervention. The blood pressure and its variability were observed and compared. Results:There were no significant differences in comparing the blood pressure and blood pressure variability between the two groups before intervention (P>0.05); after 3-month intervention, the blood pressure and its variability were significantly improved in both groups (P<0.05); the improvements in the observation group were more significant than those in the control group (P<0.05). Conclusion:Tuina manipulations of kidney-tonifying blood-circulating and collaterals-unblocking plus medication can produce a better effect than regular medication in promoting blood pressure and its variability, and this method is worth applying in clinic as it’s easy-to-operate and has no adverse effect.

Objective To evaluate the role of calcium channel in the mechanism of the generation and maintenance of bursting firing of substantia nigra pars compacta (SNc) dopaminergic neurons in rats.Methods Using the patch clamp technique,we observed the firing pattern switching features after adding 10 μmol/L N-methyl-D-aspartic acid (NMDA),compared the changes of whole-calcium current and L-type calcium current with or without NMDA,and analyzed the correlation between the generation of burst firing and L-type calcium channel activation.Results After NMDA treatment,the firing pattern of SNc dopaminergic neurons changed to burst firing,which was compromised by a charastistic high plateau potential and series of action potential on it.The current density of L-type calcium current increased significantly after adding NMDA,which,from (2.86 ±0.26) pA/pF (n =28),significantly increased to (3.75 ± 0.18) pA/pF (n =34 ; t =7.52,P =0.002 8).The high plateau potential was almost abolished with the application of verapamil,a specific antagonist of L-type calcium channel.Consiusion NMDA could induce the firing pattern changed to burst firing in SNc dopaminergic neurons,while L-type calcium channel contributes to the process of generation and maintenance of burst firing.

Objective To analyze the laboratory testing data of 2009 pandemic influenza A (H1N1) vaccines during lot release procedure, thus to know the overall quality status of this vaccines.Methods National Institutes for Food and Drug Control(NIFDC) carried out the laboratory test according to the specifications of each manufacture, and the results was analyzed and compared between manufacturer and NIFDC. Results 99.8% of vaccines batches were released by NIFDC, haemagglutinin contents were between 90% to 103 % of labeled values, and testing results slightly differ between manufactures and NIFDC,other items related to safety were all meet specifications. Conclusion The quality of H1N1 vaccines in China were satisfying, the lot release and independent test by NIFDC play important roles to ensure the vaccines' quality.

ObjectiveTo explore the reasons of local adverse effect of compressed-air aerosol inhalation for patients with pulmonary diseases,and to put forward corresponding countermeasures.Methods By convenience sampling,150 patients with respiratory diseases,treated by aerosol inhalation in Department of Respiratory Medicine in a Level Ⅲ-Class A hospital in Tianjin,were selected,and were surveyed by a cross sectional questionnaire about their general data,knowledge of aerosol inhalation,the adverse effect the patients percepted during aerosol inhalation and their countermeasures.Results Within the 150 patients,clinical symptoms of 127 had been improved,with a total effective rate of 84.67%. Local adverse effect happened to 136 patients,accounting for 90.67%,including dryness in mouth,nose or throat,bitter taste,pharyngeal discomfort, cough,etc. Hoarseness happened to four patients. Only one patient discontinued the aerosol inhalation because of long lasting serious dryness in the upper respiratory tract. Comparison of incidence rate of local adverse effect in patients′ gender,educational background,monthly income,time of aerosol inhalation and mastery of relevant knowledge showed statistical significance (P<0.05). Comparison of incidence rate of local adverse effect in patients′ age,drugs of aerosol inhalation showed no statistical significance (P>0.05).Conclusions Main reasons leading to local adverse effect during aerosol inhalation are the patients′ lack of relevant knowledge and professional guidance. So during aerosol inhalation,nursing staff should take the initiative to guide the patients technologically, so that incidence rate of local adverse effect can be lowered,and patients′ treatment compliance improved.

Objective:To translate the Understanding COPD (UCOPD) into Chinese, culturally adjust it and test its reliability and validity.Methods:According to the translation principle of Brislin questionnaire, the English version of UCOPD was translated, back-translated and culturally adjusted and pre-tested to form the Chinese version of UCOPD. From December 2020 to April 2021, a total of 101 COPD patients admitted to Tianjin Medical University General Hospital were enrolled using the convenient sampling method. The reliability and validity of the scale were tested.Results:The Chinese version of UCOPD included section A and B. Section A included 3 dimensions, such as "about COPD" "managing symptoms of COPD" "accessing help and support", with a total of 18 items. The results of exploratory factor analysis showed that the cumulative variance contribution rate of the three common factors was 59.35%. The Cronbach's α coefficient of section A was 0.893, and the Cronbach's α coefficient of each dimension was 0.756-0.864. The test-retest reliability of section A was 0.954, and the test-retest reliability of each dimension was 0.779-0.887. Section B included 6 items, which were used to measure the satisfaction of pulmonary rehabilitation courses in COPD patients. However there were big individual differences, so the reliability and validity test of section B were not evaluated.Conclusions:Section A of the Chinese version of UCOPD questionnaire has good reliability and validity. It can be used to evaluate self management ability of patients with good knowledge of COPD but less confidence in symptom control and health maintenance, so as to guide clinical medical staff to formulate accurate intervention measures to improve patients' self management.