Two methods for the determination of platinum group elements were established based on neutron activation analysis (NAA) and inductively coupled plasma mass spectroscopy (ICP-MS) combined with fire assay preconcentration. Their analytical sensitivity,accuracy and applicability were discussed.The detection limits (ng/g)of NAA for Ru,Rh,Pd,Os,Ir and Pt are 0.5,0.5,0.3,0.1,0.01 and 0.2,respectively.whereas those of ICP-MS are 0.1 for Ru,0.05 for Rh,0.1 for Ir and 0.1 for Pt. Thus, both are complimentary for determination of platinum group elements. By the established methods the contents of platinum group elements in five geological reference materials were determined.

Objective To investigate the characteristics and proper use of anticoagulants in hemodialysis (HD). Methods Thirty-one HD patients were enrolled in the study. Unfractionated heparins (UFH), dalteparin sodium or argatroban were used for HD anticoagulation respectively. Blood specimens were taken from the arterial line at the beginning (0 h) and at the end of HD (4 h), and from the arterial (2a) and the venous (2v) line respectively at 2 h of the HD session. Glass bead activated clotting time (gbACT), clot rate (CR) and platelet function (PF) were examined by Sonoclot analyzer. Prothrombin fragment 1+2 (PF1+2) and granule membrane protein-140 (GMP-140) were assayed by ELISA. Meanwhile, blood was taken from 8 healthy volunteers to examine the above parameters as control. Results (1) Compared with the control group, CR, PF1+2, PF, GMP-140 were increased significantly in all the patients (P<0.05). (2) UFH group:Compared with the 0 h point, gbACT of other time points increased significantly (P<0.05), CR, PF, and PF1+2 decreased significantly (P<0.05). Compared with the control group, gbACT increased (P<0.05) and CR decreased (P<0.05) significantly at the end of the sessions. (3) Daheparin sodium group: Compared with the 0 h point, gbACT of 2a point increased significantly (P<0.05), CR and PF1+2 of 2a, 2v and 4 h points decreased significantly (P<0.05), meanwhile, the extents of increased gbACT and decreased CR from the arterial line were greater than those from the venous line. Compared with the control group, gbACT increased significantly at the end of HD session (P<0.05), but CR was not significantly different. (4) Argatroban group: There were no significant differences of gbACT between 0 h and other time points. CR of 2a, 2v points decreased obviously than that of 0 h point, and CR of 2v decreased more significantly. CR of 2a point was not different from the control group, while CR of 4 h point was greater as compared to control group. During the monitoring, PF1 +2 tended to increase. Conclusions With intensive anticoagulant effect, UFH may induce the risk of hemorrhage not only during but also after the dialysis sessions. Dalteparin sodium, a good anticoagulant, is stir related with the risk of hemorrhage during HD. Argatroban is an ideal anticoagulant for patients with the risk of hemorrhage.

Objective To evaluate the effect of rehabilitation nursing with movement getting out of bed in early period after operation on the patients undergone the total hip replacement (THR).Methods The rehabilitation nursing was performed to 47 patients undergone THR including movement getting out of bed in early period; prepared sufficiently pre-operatively (evaluation and rehabilitation training); avoided the complications post-operatively; monitored the vital signs; trained standardizely; relieved pain sufficiently; took off the tubing as early as possible; rehabilitated with the walking aid.Results None had DVT, dislocation, fracture around the prosthesis, infections, death etc, all patients got out of bed in the 1st day after operation, the average time in hospital was 19 days, and the average Harris scores were 54.26, 62.55, 73.58, 91.17 and 93.35 at pre-operation, and in 14th day, 1st month, 3rd month and 6th month post-operation respectively.Conclusion Rehabilitation nursing with early movement getting out of bed to exercise after THR operation can promote the recovery of the hip function, reduce the complications, shorten the hospital stay.

Objective:To explore the effect of prophylactic use of anti-osteoporosis drugs on hand function in hemiplegic patients after stroke.Methods:From January 2018 to October 2019, 66 patients with hemiplegia after stroke admitted to the Third Hospital of Quzhou were selected and randomly divided into observation group and control group according to the random digital table method, with 33 cases in each group.The control group was given routine rehabilitation treatment, the observation group was given calcium carbonate D3 combined with calcitriol preventive anti-osteoporosis treatment.The two groups were treated for 12 weeks.Before and after treatment, the Brunnstrom stage score, Fugl Meyer(FMA) score, Wolf exercise score, modified Barthel index score, bone mineral density(BMD) and bone markers were compared between the two groups.Results:After treatment for 4, 8, 12 weeks, the Brunnstrom stage scores of the observation group were (2.97±0.90)points, (3.89±1.04)points, (4.68±1.04)points, respectively, which were higher than those of the control group[(2.46±0.89)points, (3.13±0.97)points, (4.09±0.97)points]( t=2.315, 3.070, 2.383, all P<0.05)and the FMA scores of the observation group were (34.28±5.17)points, (49.12±6.24)points, (55.73±6.74)points, respectively, which were higher than those of the control group [(30.07±5.05)points, (44.78±6.03)points, (50.10±6.71)points] ( t=3.346, 2.873, 3.401, all P<0.05), and the Wolf exercise scores of the observation groupwere (1.83±0.45)points, (2.91±0.64)points, (3.96±0.91)points, which were higher than those of the control group [(1.49±0.49)points, (2.28±0.57)points, (3.42±0.83)points] ( t=2.936, 4.223, 2.519, all P<0.05), and the Barthel index scores of the observation group were (59.12±5.73)points, (71.34±6.03)points, (78.98±6.89)points, respectively, which were higher than those of the control group [(55.14±5.62)points, (65.23±6.19)points, (71.54±6.80)points] ( t=2.849, 4.062, 4.415, all P<0.05). After 4, 8 and 12 weeks of treatment, BMD level of lumbar spine and hip in the observation group were higher than those in the control group, and the differences were statistically significant(all P<0.05). After 4, 8 and 12 weeks of treatment, the total amino terminal propeptides of type I collagen in the observation group were (76.02±7.26)μg/L, (58.34±6.45)μg/L, (49.12±5.12)μg/L, respectively, which were lower than those in the control group[(79.98±7.13)μg/L, (64.48±6.71)μg/L, (54.29±5.15)μg/L]( t=2.236, 3.790, 4.090, all P<0.05), and the serum osteocalcin levels in the observation group were (3.41±0.53)ng/L, (4.75±0.49)ng/L, (6.09±0.72)ng/L, respectively, which were higher than those of the control group [(3.02±0.48)ng/L, (4.16±0.47)ng/L, (5.41±0.67)ng/L]( t=3.133, 4.992, 3.972, all P<0.05)and the 25 dihydroxyvitamin D3 levels in the observation group were (34.87±5.41)ng/L, (48.71±5.67)ng/L, (72.67±6.95)ng/L, respectively, which were higher than those of the control group [(30.22±5.30)ng/L, (42.35±5.12)ng/L, (64.62± 6.14)ng/L]( t=3.527, 4.782, 4.987, all P<0.05). Conclusion:The preventive use of anti-osteoporosis drugs in patients with hemiplegia after stroke has significant effect, which can improve the function of hands and upper limbs, improve the quality of life of patients, improve the bone density and improve the bone metabolism index of patients.

Objective To observe the effects of Coix seed injection on the cell viability and radiosensitivity of human hepatoma cell line Bel-7402.Methods Bel-7402 cells were irradiated by X-rays,or treated with Coix seed injection,or treated with both of them.The cells proliferation and apoptosis were detected by MTT and by flow cytometry respectively.Cell cloning was used to observe the number of viable cells and to draw the cell survival curve.The mRNA and protein level of Bax,Bcl-2 were detected by RT-PCR and Western blot respectively.Results It was found that the Coix seed injection group (12 μmol/L) and X-ray group (8 Gy) had a significant inhibitory effect on the growth of hepatocellular carcinoma cells (t =17.03,11.26,P ＜ 0.05).And compared with Coix group and irradiation group,the combined treatment group showed higher inhibition rate (t =24.80,20.19,P ＜0.05).The mRNA and protein levels of Bax were gradually elevated (F =437.92,67.91,P ＜ 0.05),while the expressions of Bcl-2 reflected a decreased trend (F =31.18,48.50,P ＜ 0.05).The D0 values of pure irradiation group and combined treatment group were 4.27 and 3.34,respectively,and the sensitization enhancement ratio was 1.27.Conclusions The Coix seed injection inhibit cell growth and induce apoptosis,as well as increase radiative sensitization may via the apoptosis related factors Bax and Bcl-2 in hepatocellular carcinoma.

Objective: To analyze the clinical effect of fluoxetine in the treatment of patients with post-cerebral infarction depression (PSD) and its influence on neurological rehabilitation. Methods: From February 2017 to February 2018, 92 PSD patients received treatment in the department of neurology of the Third People's Hospital of Quzhou were included in the study.The patients were randomly divided into two groups according to the digital table, with 46 cases in each group.The control group was treated with specialist symptomatic therapy, while the study group was treated with fluoxetine intervention for 4 weeks.The Hamilton anxiety scale (HAMD), neurological deficit scale (NIHSS) and daily living capacity scale (ADL) were used to evaluate the clinical effects of the two groups, and the adverse reactions of the two groups were observed. Results: One week before treatment, the HAMD, NIHSS and ADL scores of the study group were (28.37±2.18)points, (23.10±3.16)points and (40.61±3.52)points, respectively, which of the control group were (28.30±2.24)points, (22.91±3.20)points and (41.15±3.35)points, respectively, there were no statistically significant differences between the two groups (t=0.223, 1.522, 0.761, all P>0.05). After 2 weeks of treatment, the HAMD, NIHSS and ADL scores of the study group were (21.08±2.33)points, (19.27±2.89)points and (49.26±2.88)points, respectively, which were higher than those of the control group[(24.15±2.43)points, (21.16±2.18)points, (44.26±2.54)points](t=4.384, 10.216, 8.276, all P<0.05). After 4 weeks of treatment, the HAMD, NIHSS and ADL scores of the study group were (12.61±1.87)points, (10.12±1.30)points, (70.13±2.16)points, respectively, which were higher than those of the control group[(15.20±2.06)points, (17.45±2.66)points, (51.19±2.46)points](t=7.273, 18.283, 5.371, all P<0.05). The total effective rate of neurological recovery in the study group was 91.30%(42/46), the total effective rate of depression treatment was 84.78%(39/46), which in the control group were 76.09%(35/46) and 65.22%(30/46), respectively, the differences were statistically significant (χ²=3.903, 4.696, all P<0.05). Conclusion The application of fluoxetine in the treatment of PSD can effectively improve the patients' depressive symptoms, promote the recovery of neurological function, improve self-care ability, and has high safety.It has important clinical value.