Objective To explore the effects of Seretide(50/250 μg)in the treatment of patients with stable moderate COPD.Methods Sixty-four patients in the stable period of moderate COPD were randomized into 2 groups,the test group and control group.The control group received routine treatment.The test group received the Seretide(50/250 μg)inhalation in addition to the routine treatment.During the 2-year observation,the pulmonary functions before and 12 and 24 months after treatment were recorded.The clinical symptoms,physical signs,the frequency of acute exacerbation of COPD and hospitaliztion duration were also graded.Results The difference of the parameters before and after the treatment was significant in the treatment group(P ＜0.01)but not in control group(P ＞0.05).The scores of symptoms and signs([2.1 ±0.4]vs.[4.4 ± 0.3],t =2.365),the frequency of acute exacerbation of COPD(29％ vs.47％,x2 =3.547)and hospitalization period ([5.0 ± 1.2]d vs.[10.0 ± 1.6]d,t =2.149)in the treatment group were significantly improved compared with control group after 24 months' treatment(all P ＜ 0.01).Conclusion Seretide(50/250 μg)is able to improve the lung function of the patients with stable moderate COPD and reduce the frequency of acute exacerbation,showing certain value in clinical practice.

The apoplexy was first formally proposed by Zhang Zhongjing in TCM.After extensive study of the medical researchers,its on causes,pathogenesis,diagnosis and treatment and prescription were developed.The cause and pathogenesis covered three stages:The period of exopathic wind,endogenous wind and combination of internal and external wind.The knowledge of the cause and the pathogenesis of the apoplexy during Jing,Yuan and Ming dynasty focused on endogenous wind.The article reviews the medical researchers' understanding of the apoplexy's pathogenic rule from the review on classical literatures of this period.

Objective To investigate the correlation between aberrant methylation status of ras association domain family 1A (RASSF1A) , transcriptional levels of DNA methyltransferases (DNMTs) and hepatitis B virus ( HBV) infection in patients with hepatocellular carcinoma ( HCC). Methods HCC samples in 61 cases were collected. Aberrant methylation status of RASSF1A was detected using methylation specific PCR (MSP). Transcriptional levels of DNMT1, DNMT3 A and DNMT3B was measured using semi-quantitative reverse transcriptase-PCR(RT-PCR). Results RASSF1A gene with abnormal methylation in initiation zone was found in 45 cases with HCC among 61 patients (73.8%). Further analysis revealed RASSF1A methylated in 32 of 47 HBV infected cases and in 13 of 14 uninfected cases. However, there was no significant association between methylation status of RASSF1A and HBV infection (x2 =2.260, P = 0. 133). Compared with normal control, DNMTs was up-regulated in all HCC samples, HCC cell lines and HBV infected group. Analysis within each group indicated that DNMT3A and DNMT3B levels of HCC increased in MSP positive cases ( t = 3. 494, P