ObjectiveTo investigate the prevalence o f erectile dysfuction (ED) in Beijing urban ared,and to study the influence of dem ographic and other risk factors.MethodsUsing systemic epidemiological method, 1247 married men in Beijing urban area were selected by two-stage sample methods.General condition, health status, medications, smok ing and lifestyle was collected by trained interviewers.A self-administered sex ual activity questionnaire (IIEF-5) was used to characterize erectile potency. ResultsThe prevalence rate of ED of married men in Bei jing is 39.1%,and the prevalence rate of ED over 40 years is 54.5%.ED was strong ly correlated with age,especially for men over 60 years.The prevalence rate of c omplete ED in 60～69 years group is nearly 5 times as in 50～59 years group.The ot her important factors correlated with ED include diabetes,vascular disease and s o on.ConclusionsThe prevalence of ED in Beijing urban area and the important risk factors are similar to those reported.Whereas the pr evalence rate of complete ED over 60 years is significantly higher.

It was an dideal model of respiratory distress syndyomeinduccd in 59 rabbits by Ⅳ oleic acid injection. Pulmonary hemodynamics, cardiac impedance paramcters were measured, microemboli and leukocytcs were abtained directly from the pulmonary microcirculation by use of the retrograde perfusion method. The results showed that pulmonary hemodynamics were changed significantly, but did not correlate with development of pulmonary edema. It was found that basic impedance (Zo) increased, stroke volume (SV), cardiac output (CO) and cardiac index (CI) all decreased (P

Objective To study the polysaccharides from dried rhizome of Ligusticum chuanxiong.Methods DEAE-Cellulose and gel-filtration chromatography were used to isolate and purify the polysaccharides,whose structures were characterized by chemical and spectral methods.Results Four homogeneous polysaccharides,named LCP-1,LCP-2,LCP-3,and LCP-4 were obtained from the water extract of L.chuanxiong.Four structures were investigated to be complicated polysaccharied fractions,with the molecular weights of 3.1?10~4,5.2?10~4,9.0?10~4,and 3.6?10~4,respectively,then estimated by GPC.Conclusion The four polysaccharides are obtained from this plant for the first time.

Objective To investigate the expression of tumor necrosis factor-related apoptosis-induce ligand (TRAIL) receptors in bla dder transitional cell cancer. Methods TRAIL receptors m RNA were tested by means of RT-PCR and Northern Blot in bladder transitional ce ll cancer tissue. Results The expression of death recept or 4 (DR4) and death receptor 5 (DR5) have been certificated in bladder transiti onal cell cancer and normal bladder tissue,decoy receptor 1 (DcR1) expressed onl y in normal bladder tissue. Conclusions TRAIL gene may p lay an important role in the apoptosis of bladder transitional cell cancer.

Objective To verify whether the extracellular domain of flt 1 has anti angionesis activity in vivo. Methods A recombinant plasmid pcDNA 3.1/flt 1 n3 was transfected into the human bladder carcinoma EJ cell by lipofectamin, which was constructed by inserting the N terminal first three IgG like domain of VEGF receptor flt 1 (flt 1 n3 ) into eukaryotic expression vector pcDNA 3.1. Results The rflt 1 was functionally expressed in stably transfected EJ and the product secreted in the medium could be specifically bind to rhVEGF165.The result showed that the bladder cancer transfected with rflt 1 had a lower microvessle density than the control. Conclusions It is proved that the expressed rflt 1 n3 can inhibit tumor growth and angiogenesis in nude mice model.

Objective To investigate the correlations of serum matrix metalloproteinase-2, -9 (MMP-2, MMP-9) contents and tissue expressions in hepatocellular carcinoma with tumor invasion and metastasis. Methods Serum MMP-2, MMP-9 contents were detected in 40 patient with hepatocellular carcinoma and 20 healthy controls by ELISA; the expressions and distributions of MMP-2 and MMP-9 in 40 patients and 10 normal tissues were detected by immunohistochemical method. Results Serum MMP-2, MMP-9 contents were significantly elevated in cancer samples compared with normal serum (P

Objective To investigate the expressions of CD40 and ICAM-1 in primary hepatocarcinoma and clinical significance and analyze their relationships with carcinogenesis and progression of liver cancer. Methods S-P immunohistochemistry was used to detect the expressions of CD40 and ICAM-1 in 40 cases of hepatocarcinoma and 25 cases of normal liver tissue (distance from cancer tissues≥2 cm).Results The positive expression rates of CD40 and ICAM-1 were 47.5% and 82.5% in liver cancer tissue,which was significantly higher than those in normal liver tissue (P0.05).The positive expression rate of ICAM-1 was associated with lymph node metastasis as well as histological grade(P

Objective To study the influencing factors of postoperative cognitive dysfunction ( POCD) in elderly patients after knee joint replacement. Methods 165 cases of elderly patients undergo-ing knee replacement surgery were recruited in the study. According to whether there was POCD,the patients were divided into POCD group (35 cases) and non POCD group (130 cases).The gender,age,educational level,medical history,physical exercise,operation mode( cruciate-retaining total knee arthroplasty and poste-rior stabilized total knee arthroplasty) ,operation time,blood loss,preoperative depression score and postoper-ative pain score were recorded and compared.The logistic multiveariate regression analysis was conducted to analyze the risk factors of POCD in elderly patients with knee replacement. Results (1)There were statis-tically significant differences in the total score of MoCA, visual space and executive function,naming and de-laying recall between non-POCD group and POCD group after the operation ( Z=-5. 327, P=0. 000;Z=-3.140, P=0.002;Z=-2.910, P=0.004;Z=-3.333, P=0.001). (2)The results of logistic multiveariate regression analysis showed the age>70 years old ( OR=3.708,95%CI =1.037-13.261, P<0.05) ,hyperten-sion ( OR=5.250,95%CI =1.787-15.423, P<0.05),diabetes ( OR=6.560,95%CI =1.418-30.345, P<0.05) ,preoperative depression ( OR=4.648,95%CI =1.290-16.744, P<0.05) and postoperative pain score>4 ( OR=3.149,95%CI=1.072~9.251, P<0.05) were risk factors of POCD,and physical exercise ( OR=4.330,95%CI =1.294-14.488, P<0.05) and CR-TKA( OR=0.187,95%CI =0.045-0.781, P<0.05) were the protective factors of POCD. Conclusion The overall level of cognitive function in elderly patients after knee replacement surgery is low. The advanced age,hypertension,diabetes,lack of physical exercise,choice of operation method,postoperative pain and preoperative depression are important influence factors of POCD.

Objective To evaluate the efficacy of testosterone supplementation for partial androgen deficiency in middle-aged men. Methods This prospective,double-blind,placebo-controlled,randomized clinical trial was conducted in our hospital.A total of 179 patients (mean age,41.8 years) were randomly divided into study group (n=136) and control group (n=43).The 136 patients in study group received andriol (80 mg,twice a day) for 3 months;while the 43 patients in control group received no special treatment.The changes of partial androgen deficiency in aging male (PADAM) scores,serum total testosterone (TT),free-T (fT) and relevant sexual hormone levels were assessed before and after treatment. Results In study group the PADAM scores at 1 and 3 months after treatment were significantly improved compare with those before treatment (P0.05). Conclusions Partial androgen deficiency in middle-aged men is clinically exist and testosterone supplementation for this condition is safe and effective.

Bioavailable studies were performed based on plasma concentrations of captopril in 5 Beagle dogs and 5 male healthy volunteers after a single oral administration of captopril sustained-release tablet and sugar-coated tablet. A one-compartment model was adopted. Relative bioavailability of sustained-release tablet to sugar-coated one was 131.6% for dogs and 111.0% for humans. Their mean residence times (MRTs) were 4.52 h and 1.96 h in dogs, 4.28 h and 2.77 h in humans, respectively. The maximum concentrations were 995.9 ng/ml and 2470.8 ng/ml in dogs, 126.2 ng/ml and 251.2 ng/ml in humans for two kinds of tablets, respectively. The duration time, in which plasma concentration staved above 50% inhibitory concentration of angiotensin converting enzyme activity, was more than 10 h for sustained-release tablets and 6h for sugar-coated tablets at the same dose (37.5 mg). consequently, it could be expected that the sustained-release tablet dosed twice a day should have a greater efficiency than marketed sugar-coated tablet taken 3 times daily.