Twety dogs were used to study a high Power Co_2 laser scapel for hemisplenectomy and traumatic rupture of the spleen by temporary occlusion of the spleen pedicle. The laser scapel with its point power of 160-200 w was used for a focusing cut and for stopping bleeding by the laser beam beyond the focus. The wound surface could be dried and coking good, without injecting adrenaline into the splenic artery. All the dogs survived after the experimental investgation and no infection or any more Post-oPerative bleeding was found. The spleen membrane was formed on the section of the spleen 1 year after operation. Compared with other operations for keeping the organ of spleen, this method safer and more reliable. It could be used in clinical practice with success.

Objective To observe the clinical efficacy of improved closed repair operation combined with YiQiZengYe traditional Chinese medicine(TCM) for treating the patients with severe rectocele.Methods 60 patients with severe rectocele were randomly divided into treatment group and control group.The patients in treatment group received the treatment of improved closed repair operation combined with YiQiZengYe TCM,and the patients in control group received the treatment of conventional transvaginal incision repair.The clinical efficacy was evaluated 3 months after surgery.The clinical symptom score,rectocele depth of resting bit and force row,postoperative complications of two groups were comparatively analyzed.Following-up of 6 months after surgery,the postoperative recurrence was observed.Results The effective rate of the treatment group was 100.0％,and was significantly higher than that of the control group(70.0％) (x2 =10.588,P ＜ 0.05) ; The complication rates of postoperative bleeding,pain,anal bulge and infection in the treatment group were 0 cases,3 cases(10.0％),7 cases(23.3％),1 cases (3.3％),respectively,wich were significantly lower than those of the control group [4 cases (13.3％),23 cases (76.7％),28cases (93.3％),9 cases (3.3 ％)] (x2 =4.286,38.400,30.240,7.680,all P ＜ 0.05).There was no postoperative recurrence in the treatment group.Conclusion The improved closed repair operation combined with YiQiZengYe TCM for treating the patients with severe rectocele can significantly improve the clinical efficacy,effectively improve prognosis and prevent recurrence.This treatment method is safe and reliable.

It was an dideal model of respiratory distress syndyomeinduccd in 59 rabbits by Ⅳ oleic acid injection. Pulmonary hemodynamics, cardiac impedance paramcters were measured, microemboli and leukocytcs were abtained directly from the pulmonary microcirculation by use of the retrograde perfusion method. The results showed that pulmonary hemodynamics were changed significantly, but did not correlate with development of pulmonary edema. It was found that basic impedance (Zo) increased, stroke volume (SV), cardiac output (CO) and cardiac index (CI) all decreased (P

AIM and METHODS: To explore the role of telomerase reverse transcriptase (TERT) in monocrotaline-induced pulmonary hypertension (PH), TERT was investigated by using immunohistochemistry and image analysis on the chronic PH induced by MCT in Sprague-Dawley rats. RESULTS: TERT was positive in pulmonary vascular smooth muscle cells, bronchial smooth muscle cells and chondrocytes in normal group. TERT in MCT group was more positive in pulmonary vascular smooth muscle cells and bronchial smooth muscle cells compared with normal group. CONCLUSION: Over expression of TERT in pulmonary vascular smooth muscle cells and bronchial smooth muscle cells is related to MCT-induced PH.

Loss of cerebellar Purkinje cells,neurons of brain stem and degeneration of spinal ciruits are major morphologic changes in most patients with ataxia.Studies on spinocerebellar ataxia have focused on pathogenesis in recent years. Different genotypes have been identified by genolineage analysis and gene study. This study reviews the literatare on the classification,clinical features and pathogenesis of spinocerebellar ataxia.

[Objective] To evaluate the curative effect of anterior cruciate ligament(ACL)reconstruction for remaining anteromedial or posterolateral bundle and ruptured fiber.[Method]Among 89 cases of ACL rupture,there were 32 cases of sports injury,24 cases training injury,23 cases traffic accidents and 10 cases sprained in daily life.ACL ruptured at the notch of the femur in 24 cases,and at the intercondyle of the tibial in 28 cases.Thirty-two cases were reconstructed with quadri-bundle Hamstring tendon knot press-fit technique and 20 cases were reconstructed with quadriceps tendon press-fit technique with autogenetic patellar bone.ACL anteromedial bundle(AMB)ruptured in 14 cases and posterolateral bundle(PLB)ruptured in 23 cases.The intact bundle and the remnants of ACL were preserved.AMB and PLB were reconstructed with double-bundle Hamstring tendon knot press-fit technique in 37 cases.Absorbable interference screws were used at the tibial tunnel and sutured tendon was knotted across the tibial bone bridge.[Result]The average duration of follow-up were 25 months(14-37 months).Lysholm knee function score increased by 27.7 scores,from average 67.5 scores of pre-operation to average 95.2 scores at the end of the follow-up.Rulermetr examination tibial translation was 4 mm?2 mm in 72 cases and 6 mm?3 mm in 17 cases.Lachman test was negative in 79 case,weakly positive in 8 cases and positive in 2 cases.The function of the knees were improved and the stabilization was strengthened after operation.According to knee therapeutic effect evaluation criterion,76 cases were excellent and 13 cases were good.[Conclusion]To keep the remaining of anteromedial or posterolateral bundle and ruptured fiber for ACL reconstruction is beneficial for the graft revascularization or healing,and is helpful for the position sense or rehabilitation.

Objective: The study was designed to observe the effects of alanyl-glutamine (Aln-Gln) dipeptide on postoperative intestinal permeability and systemic inflammatory response.Methods: A prospective,randomized and controlled trial was taken.20 patients who underwent abdominal surgery were randomized into two groups: study group (intravenous administration of Aln-Gln dipeptide,0.5 g /(kg·d),for 4 days,n=10) and control group (equal normal saline as placebo,for 4 days,n=10).Temperatures,heart rates and respiration rates of all patients were daily recorded during administration.The white blood cell counts ,serum concentrations of glutamine (Gln),diamine oxidase (DAO) and interleukin-6(IL-6) and urine lactulose/mannito (L/M) ratio were measured before and after operation.Results: Serum Gln concentration was significantly decreased in control group and increased in study group on postoperative day 5.Urine L/M ratio,serum concentrations of DAO and IL-6 were significantly increased in control group and decreased in study group.The morbidity of SIRS was significantly decreased in the study group and the score of SIRS was also lower than in the control group.Conclusions: Administration of Aln-Gln dipeptide can increase the level of serum Gln,decrease the intestinal permeability,maintain the intestinal barrier and attenuate the systemic inflammatory response in the early period of postoperative patients.Aln-Gln dipeptide can be used in the fast track surgery to help patients recover rapidly.

Objective To investigate the present mental disability situation in Hubei provinee and the reha- bilitation strategies in use. Methods Provincial data from the Second China Sampling Survey of Disability was ana- lyzed. The 31 districts sampled in the survey were divided into a city group and a countryside group. The incidence of mental disability reported in the two groups was compared. Results Reported mental disability was found to be sig- nifieantly more prevalent in the countryside than in the city. However, the proportion of severe mental disability was significantly lower. Conclusion The need for rehabilitation of mental disability is huge in Hubei province. Society and the government should pay more attention to the rehabilitation of mental disability.

Objective: The study was designed to observe the effects of alanyl-glutamine (Aln-Gln) dipeptide on postoperative intestinal permeability and stress response. Methods: A prospective, randomized and controlled trial was taken. 20 patients who underwent abdominal surgery were randomized into two groups: study group [intravenous administration of Aln-Gln dipeptide, 0.5 g/(kg?d), for 4 days, n=10 and control group (normal saline as placebo, for 4 days, n=10)]. Serum concentrations of glutamine (Gln), diamine oxidase (DAO), cortisol, interleukin-6(IL-6) and urine lactulose/mannito (L/M) ratio were measured before and after operation. Results: Serum Gln concentration was significantly decreased in control group and increased in study group on postoperative day 5.Urine L/M ratio was significantly increased in control group and decreased in study group.The serum concentrations of DAO,cortisol and IL-6 were significantly decreased in study group while compared with control group. Conclusion: Administration of Aln-Gln dipeptide can increase the level of serum Gln,decrease the intestinal permeability and attenuate the stress response in the early period of postoperative patients. Aln-Gln dipeptide can be used in the fast track surgery.

Objective: The study was designed to observe the effects of alanyl-glutamine(Aln-Gln) dipeptide on postoperative intestinal permeability and immmunologic function in abdominal patients. Methods: A prospective,randomized and controlled trial was taken.20 patients who underwent abdominal operation were divided into two groups: study group(intravenous administration of Aln-Gln dipeptide,0.5 g/(kg?d),for 4 days,n =10) and control group(equal normal saline as placebo,for 4 days,n =10).Serum concentrations of glutamine(Gln) and diamine oxidase(DAO),blood total lymphocyte count(TLC) and human leukocyte antigen(HLA)-DR and urine lactulose/mannito(L/M) ratio were measured before and after operation. Results: Serum Gln concentration was significantly decreased in control group and increased in study group on postoperative day 5.Urine L/M ratio and serum DAO level were significantly increased in control group and decreased in study group after operation.Blood TLC and HLA-DR expression in the study group were significantly higher than those in control group on postoperative day 5. Conclusion: Intravenous administration of Aln-Gln dipeptide can increase the serum Gln level,decrease the intestinal permeability,maintain the intestinal barrier and improve the immmunologic function in the early period of postoperative patients.Aln-Gln dipeptide can be used in the fast track surgery to help patients recover rapidly.