BACKGROUND:According to worldwide statistics, internal fixation was commonly selected to treat patients with distal femoral fractures and acromioclavicular joint dislocation. The firmness of Kirschner wire is poor during fixation, so needle withdrawal easily appears, and the fixation would be failure. To reduce complications after fixation, AO/ASIF clavicular hook plate as a novel fixator has been used in treatment of distal clavicle fractures and acromioclavicular joint dislocation. OBJECTIVE:To explore the safety and effectiveness of AO/ASIF clavicular hook plate in the treatment of distal clavicle fractures and acromioclavicular dislocation, and to compare with Kirschner wire tension band.METHODS:100 patients with distal clavicle fractures and acromioclavicular joint dislocation, who were treated in the Department of Orthopedics of Tongcheng People’s Hospital from June 2010 to December 2013, were enrol ed in this study. They were equal y assigned into Kirschner wire tension band group and AO/ASIF clavicular hook plate group according to different fixation methods. The excel ent and good rate of shoulder function recovery, shoulder function score and upper limb functional recovery were observed and compared between the two groups after fixation. RESULTS AND CONCLUSION:The excel ent and good rate of shoulder function recovery was 98%in the AO/ASIF clavicular hook plate group after fixation, which was significantly higher than Kirschner wire tension band group (64%). The incidence of acromioclavicular re-dislocation was 0 after removal of fixator in the AO/ASIF clavicular hook plate group, which was lower than Kirschner wire tension band group (12%), showing significant differences between groups (P<0.05). Japanese Orthopaedic Association score was significantly higher at 5 and 10 weeks after fixation in both groups compared with pre-fixation. Japanese Orthopaedic Association score was significantly higher in the AO/ASIF clavicular hook plate group than in the Kirschner wire tension band group (P<0.05). The ranges of motion of adduction, abduction, flexion and extension were significantly higher in the AO/ASIF clavicular hook plate group than in the Kirschner wire tension band group at 10 weeks after fixation (P<0.05). Above results suggested that AO/ASIF clavicular hook plate is a safe effective fixator for repair of distal clavicle fractures and acromioclavicular joint dislocation, and characterized by smal incision, reliable fixation, and early shoulder activities.

Objective To observe the capability of inducing cerebral ischemia tolerance of Zingibers aqueous extract. Method Comparing the volume of cerebral infarction and the level of neurologic impairment in the model of middle cerebral artery occlusion in rats. Result The volume of cerebral infarction is (120.6?21.57)mm3 and (155.9?21.34)mm3 in the preconditioning group by Zingibers aqueous extract and control group respectively (P

Neuropathy pain, or neuropathic pain (NP), which pathogenesis is complex and is lack of effective treatment, is one of common chronic pain in clinic. Studies of traditional Chinese medicine (TCM) in treating NP were increased in recent years. In the review, studies of treatments of TCM compound and monomer in NP during last decade were collected and analyzed to find out possible mechanisms, and some existing problems.

One leading research target of modern tumor radiotherapy is to increase radiosensitization of tumor and improve curative effect of radiotherapy.Histone deacetylase inhibitors are epigenetic drugs that can play a part in radiosensitization through means of induction of apoptosis,inhibition of repair of DNA doublestrand breaks,cell cycle arrest,improvement of tumor cell hypoxia and increase of reactive oxygen species.There is an urgent need to develop biomarkers based on these pathways,which can promote clinically individualized treatment.

ObjectiveTo explore the possible molecular mechanisms of β-elemene combined with cisplatin and heat therapy for killing A549 cell line.MethodsThe protein expressions of Stat3,p21 Waf1/Cip1 and Survivin were detected with Western blot after treatment with β-elemene of different concentrations combined cisplatin and heat therapy.ResultsThe protein expressions of Stat3,and pStat3 and p21Waf1/Cip1 in A549 cell line were enhanced with increasing concentrations,and there was significant difference in the expressions between high and low concentration,but Survivin protein had no change at 37°C.After adding 4 μg/mL cisplatin,the expressions of p21Waf1/ Cip1,Survivin,Stats and pStat3 were reduced at β-elemene of high concentration.At 42°C,there was no significant difference in expression of Stat3 protein at 60 μg/mL elemene,but the expressions of pStat3,p21Waf1/Cip1 and Survivin proteins had sharply declined.When using 15 μg/mL elemene combined with 4 μg/mL cisplatin,the protein expressions of Star3 and pStat3 increased,and Survivin expression decreased.ConclusionsAt temperature of 37°C,β-elemene of high concentration may inhibit growth of A549 cells by higher expression of p21Waf1/Cip1 protein,and mainly by inhibiting expressions of Stat3 and pStat3 and Survivin after combined with cisplatin.At temperature of 42°C,β- elemene of highconcentrationmaypromoteapoptosispossiblythroughinhibition ofStat3 phosphorylation and expression of Survivin protein.β-elemene of low concentration combined with cisplatin leads to synergy killing effect by reducing expression of Survivin protein.

BACKGROUND: The operation on cervical spondylosis is usually done through anterolateral cervical vertebrae to mainly expose cervical vertebrae,intervertebral disc, hook joint and so on. The recent reports are mostly local studies. The observation data of the distance between cervical vertebrae and the corresponding nervus vascularis is limited.OBJECTIVE: The relation of the blood vessels and nerves to cervical vertebrae was observed in neck so as to prevent iatrogenic blood vessels and nerve injuries in the operation on cervical spondyloisis.DESIGN: A single sample trial based on the anatomic samples SETTING: The department of anatomy of a medical college PARTICIPANTS: The experiment was conducted in the Department of Anatomy of Changzhi Medical College from July 2003 to May 2004. Twenty adult antiseptic samples with 40 sides including 36 from males and 4 from the females supported by the Department of Human Anatomy, Changzhi Medical College.METHODS: The accompanying relation of the blood vessels to the nerves,the length of the blood vessels, and the distances from their starting point and end to the neighboring cervical vertebrae were observed and measured in 40 adult antiseptic samples of the neck.the distances from their starting point and end point to the neighboring cervical vertebrae.RESULTS: Totally, 40 sides of 20 samples entered the stage of the result analysis. The facial artery followed the lingual nerve, and the length from its starting point to the neighboring cervical vertebrae C3 was ( 18.5 ± 7.2) mm. The lingual artery followed the hypogolossal nerve, and the distance from its starting point of the lingual artery to C2 -3 was( 19.7 ± 8.4)mm. The superior thyroid artery followed the lateral branch of the superior laryngeal nerve, and the distance rom its starting to the point of the superior thyroid artery to C3 was (17.0 ± 5.7) mm, and the length from the end of the superior thyroid artery to C5 was (18.2 ± 2.5 ) mm. The superior laryngeal artery followed the medial branch of the superior laryngeal nerve, and the distances from its starting point and end point to C3 were( 15.7 ± 6.7) mm and(19. 7± 5.8) mm respectively. As for the inferior thyroid artery, the distances from its starting point and the end point to C7 were ( 17.3 ± 5.5 ) mm and ( 11.3 ± 3.4 ) mm respectively.CONCLUSION: In the operation on cervical spondylosis, attention should be paid to the anatomic stricture of the special position at different positions so as to reduce the iatrogenic blood vessels and nerve injuries.

PurposeThe restriction of strain ratio (SR) in the diagnosis of thyroid malignant tumor are caused mainly because of disadvantages including low sensitivity, in this research the authors used different calculation methods of strain ratio based on the size of tumors, to explore the clinical application value of them on the differentiation of benign and malignant thyroid nodules.Materials and Methods Data of 336 patients with thyroid nodule (340 nodules) who underwent elasticity imaging and ultrasound-guided fine-needle aspiration were retrospective analyzed, with the pathology combined follow up acted as golden standards, fixed surface area assay and true surface area assay of strain ratio were compared on the differential diagnosis of benign and malignant thyroid nodules with various size.Results Among 340 thyroid nodules, there were 91 small nodules and 249 big nodules. Pathological results showed that 36 of 91 small nodules and 104 of 249 big nodules were malignant, while the others were benign. The true surface area assay was significantly better for the diagnosis of smaller thyroid nodules with longitudinal diameter and transverse diameter<8 mm with higher consistency with pathology (χ2=20.89,P<0.01), and fixed surface area assay is more suitable of thyroid nodules with longitudinal diameter and transverse diameter>8mm (χ2=57.08,P<0.01). The sensitivity, specificity and consistency for the diagnosis of malignant nodules of true surface area assay were 57.10%, 83.00% and 72.30% (Kappa=0.413), which were 73.60%, 85.00% and 72.30% (Kappa=0.590) in the fixed surface area assay, and 85.00%, 85.50% and 85.30% (Kappa=0.699) in the selective usage of two methods according to the tumor size, respectively.Conclusion The sensitivity is highest when using the two methods selectively according to the nodule size, with a better consistence rate with pathological results as well.

Objective To compare the difference between a vertical line (AA) drawn to the line connecting the inner edge of the patellar tendon with the mid-point of the ending point in the posterior cruciate ligament, tibial posterior condylar line (PC), tibial plateau anterior line (AC), the maximal mediolateral distance (MMLD) and a vertical line (BB) drawn to aligning the mid-point of ending point in the posterior cruciate ligament with the medial 1 / 3 of the patellar tendon relative to the surigical transepicondylar axis (STEA) by MRI, and to explore a reliable reference to determine tibial component rotation in total knee arthroplasty , and whether it will change in knees with varus deformity. Methods Thirty healthy volunteers (Group1) and thirty osteoarthritis patients (Group2) were enrolled in this study. The angles were measured among the five tibial rotation axes and STEA after MRI. Results The angles were (-1.48 ± 2.38)°, (6.16 ± 4.53)°, (6.45 ± 5.24)° ,(5.08 ± 4.99)° and (3.24 ± 2.68)° respectively in group 1 and (-1.88 ± 2.21)°, (-3.13 ± 4.66)°, (11.13 ± 5.72)°, (4.11 ± 4.15)° and (5.12 ± 4.87)° respectively in group 2. The angle between AA and STEA was not affected by varus deformity (P > 0.05), but the others were (P < 0.05). Conclusion The angle between AA and STEA is the smallest which is used to determine tibial component rotation in knees with varus deformity is the most reliable one.

[Objective] To explore the mechanism of fever in damp- heat syndrome. [Methods] Hyperlipemia rabbit models were established by feeding fatty - sweet diet. After the injection of endotoxin, the secretion of tumor necrosis factor and interleukin 1 was observed in rabbit model (Group A) and compared with normal rabbits (Group B) . [ Results] The peak of tumor necrosis factor and interleukin 1 secretion was decreased and the decline was slow in Group A. [ Conclusion ] The damp - heat syndrome in seasonal febrile disease is not merely a simple addition of damp and heat, but a result of retention and interaction of the two factors.

Objective:To provide chemical basis for processing of leech through investigating the variations of composition of leech scalded with talc powder. Methods: Amino acids were determined by HPLC post column derivatization and steroids by GC MS. Results: The results showed the total contents of amino acids in scalded leech were reduced and the steroids of it were changed. Conclusion: The processing method of leech used at the moment has a great influence in chemical components of leech.