Objective To discuss the disposition feature of pathogenic bacterium in female urinary tract infection(UTI) ,so as to elevate the level of clinical diagnosis and therapy. Methods To cultivate medistream urine,assess vaginal secretions in the aspects of bacteriology, mycoplasma, chlamydia, mycetes and parasite. All specimen collected from 129 female patients who chiefly complained irritation of urinary tract ,from January 2003 to December 2006. Results Pathogenic microorganisms that found in the 129 female patients with UTI are gram-negative bacteria( 53.49 % ), gram- positive bacteria ( 19.38 % ), mycoplasma ( 14.73 % ), mycetes (9.30 % ), chlamydia (4.65 % ),parasite(1.55% ). Among them, escherichia coli, klebsiella pneumoniae bacilli, enterococcus faecalis, staphylococcus aureus are common species. Sexually transmitted disease(27.91% ) include the infection of diplococcus gonorrhoeae,mycoplasma and ehoamydiae. Among them,non-gonococcal urethritis is common. Combined infection(17.38 % ). Infection combined with mycoplasma urealytium and other pathogen is the most, these patients are most young or middle aged. Conclusion Pathogen in female UTI is mostly gram-positive bacteria. STD and combined infection should be paid attention to by clinicians. We suggest female UTI patients take etiological test regularly,so as to elevate the level of clinical diagnosis and therapy.

Objective To investigate the relationship between carotid plaque and serum C reactive protein(CRP) levels, leukocyte count in patients with acute cerebral infarction(ACI) Methods Carotid duplex examination was performed in 121 patients with ACI by an Advanced Technology Laboratories HDI (high definition imaging) 5000 triplex system Serum CRP was measured by nephelometry Results of carotid ultrasonography were divided into two groups: M1: normal (IMT 0 05) The number of patient with elevated CRP levels was increased in the M2 group( P

A case of ear mold impression middle ear foreign body was reported. In the exploratory operation we found the ear mold impression surrounded the ossicular chains. During the process of removal the middle ear foreign body, the incudostapedial joint was protected from obvious external force and ossicular chains were kept integrity. Tympanoplasty was completed after management of foreign body. The patient recovered well postoperatively and was discharged as scheduled.
Ear Ossicles ; surgery ; Ear, Middle ; Female ; Foreign Bodies ; surgery ; Humans ; Middle Aged ; Tympanoplasty

Purpose: There is no appropriate instrument or devices and corresponding technological specification for measurement of output dose and dose disdribution in stereotactic radiothrapy. Small field of the stereotatic radiotherapy can't be measured by normal ionization chamber for its characteristics.A practible instrument and method were developed for dose measurement in stereotactic irradiation.Materials and Methods:A diode detector with 1mm sensitivety volume and a 0.3cc graphite chamber developed by us are used to measure the dose output & dose distribution in warious phantom for the beams used in stereotactic irradiation.Results:The dose outputs and dose ditributions measured by SCD-61 diode developed by us were tested and verified by TLD dosemeter,and the results show tha there is a 5% consistency for the value at the focus point.Conclusion: the diameter of the detector used for measurement must be smaller than the halt size of the radiation beams to be measured. A diode detector with 1mm sensitivity volume is the best choice for the dosimetric measurements for smaller beams used in stereotactic irradiation.

Objective To evaluate the feasibility and indications of endoscopic submucosal dissection (ESD) by analyzing the early gastric cancer cases.Methods All the pathological materials of radical gastric resections in Huashan hospital from 2007 to 2011 were retrieved and the EGC cases were selected.All the cases in EGC group which met the extended ESD criteria were reviewed.Extended ESD criteria were cited from Treatment guidelines for gastric cancer in Japan edited by JGCA in 2001.The risk factors relevant with regional metastasis,such as invasive depth and differentiation degrees,were also analysed.Results In the recent five years,there were 1159 cases of gastric cancer undergoing radical gastrectomy in Huashan hospital including 210 EGC.According to the extended criteria of JGCA,151 cases could be candidates for ESD while 126 cases could be candidates for ESD according to the typical criteria for ESD.The local metastases of lymphatic or vascular involvement for mucosa cancer,muscularis mucosae cancer,sm1 and sm2 cancer were 6.75％,15.00％,23.25％,and 39.62％ respectively (P ＜0.05).Twenty-five cases had local metastases that met the extended criteria for ESD.Within the 210 EGC cases,the local metastases of differentiated type and undifferentiated type were 13.95％ and 47.37％ respectively (P ＜ 0.05).Conclusion Most EGC cases undergoing radical gastrectomy can be cured by ESD.Its metastases are highly correlated with invasion depth and differentiation,so indications of ESD should be assessed.

AIM　To study the effects of Aβ25～35 and Apo E4 on neuronal intracellular free Ca2+(［Ca2+］i). METHODS　Hippocampal and cortical neurons suspension of newborn(0～3 days) SD rats was produced. After incubated with fura-2/AM,the neurons suspension was divided into four groups: control, Aβ25～35, Apo E4, Aβ25～35+Apo E4. Each groups ［Ca2+］i was measured using a RF-5000 dual wavelength spectrofluorometer after incubated with double distilled water, Aβ25～35, Apo E4, Aβ25～35+Apo E4 for 3 min, respectively. The neurons outocorrelation function(ACF) of the scattering light intersity was analyzed by the microscope quasi-elastic light scattering(MQLS) technique The frequency shift line width by ACF. The Γ can sympolize the cell menbrane flilidity. RESULTS　Both Aβ25～35 and Apo E4 could significantly enhance hippocampal and cortical neurons rest ［Ca2+］i, furthermore, the effect of 5 μmol*L-1 Aβ25～35 was higher than the effect of 1 μmol*L-1 Aβ25～35 (P<0.05), and they also amplified KCl-induced rise in ［Ca2+］i in hippocampal and cortical neurons(P<0.05). The interaction of Aβ25～35 and Apo E4 could also significantly enhance hippocampal and cortical neurons rest ［Ca2+］i andamplified KCl-induced rise in ［Ca2+］i in hippocampal and cortical neurons(P<0.05), but they had no synergic or additive effect.The frequency shift line widith Γ of both hippocampal and cortical neurons were decreased by both Aβ25-35 and ApoE4. CONCLUSION　Aβ25～35 and Apo E4 could enhance neuronal intracellular free Ca2+, amd decrease meirpma; ,e,brame f;iodotu. But their interaction had no synergic or additive effect. It suggested that the amplified effect of Aβ25～35 and Apo E4 on neuronal ［Ca2+］i and membane fluidity may be relative to their neurotoxity.

AIM To study the effects of A? 25～35 and Apo E4 on neuronal intracellular free Ca 2+ ([Ca 2+ ] i). METHODS Hippocampal and cortical neurons suspension of newborn(0～3 days) SD rats was produced. After incubated with fura 2/AM,the neurons suspension was divided into four groups: control, A? 25～35 , Apo E4, A? 25～35 +Apo E4. Each groups [Ca 2+ ] i was measured using a RF 5000 dual wavelength spectrofluorometer after incubated with double distilled water, A? 25～35 , Apo E4, A? 25～35 +Apo E4 for 3 min, respectively. The neurons outocorrelation function(ACF) of the scattering light intersity was analyzed by the microscope quasi elastic light scattering(MQLS) technique The frequency shift line width by ACF. The ? can sympolize the cell menbrane flilidity. RESULTS Both A? 25～35 and Apo E4 could significantly enhance hippocampal and cortical neurons rest [Ca 2+ ] i, furthermore, the effect of 5 ?mol?L -1 A? 25～35 was higher than the effect of 1 ?mol?L -1 A? 25～35 ( P

BACKGROUND: As a bone graft, coralline hydroxyapatite (CHA) has uniform and interacted pore structure, which is suitable for vascular regeneration, bone regeneration and bone deposition. It displays good biocompatibility and no immunogenicity. OBJECTIVE: To explore the clinical results of CHA in the treatment of benign osteolytic bone defects. DESIGN, TIME AND SETTING: A retrospective analysis was performed in the Department of Orthopaedics, Affiliated Hospital of Hainan Medical College from May 1996 to May 2007. PARTICIPANTS: A total of 32 cases of benign osteolytic bone defects were enrolled from Department of Orthopaedics, Affiliated Hospital of Hainan Medical College. Pathological diagnosis showed that the cause of bone defect included bone cyst for 8 cases, fibrous hyperplasia of bone for 9 cases, aneurysmal bone cyst for 4 cases, osteoenchondroma for 1 case; fracture complication for 5 cases consisting of femoral complete fracture for 2 cases and humeral complete fracture for 1 case, and humeral incomplete fracture for 2 cases. CHA was prepared by the Biomaterials Laboratory of Hainan Medical College. METHODS: According to routine approach of the operation, 32 cases of benign osteolytic bone defects were implant with CHA granules, chips or blocks after deleting tumor tissue and thinning cortical bone. Then the periosteum was sutured. Three cases with complete fracture received internal fixation, while other cases were untreated. None was fixed with plaster for external fixation. MAIN OUTCOME MEASURES: The X-ray films were observed to evaluate the bone healing at different time. RESULTS: All cases were followed up for average 6-24 months. No general abnormal reactions were found. The incisions were healed in two weeks. The lesion range of bone defect was from 3 cm?2 cm?2 cm to 12 cm?4 cm?4 cm before operation; At 1-3 months after operation, bone graft began to fuse with bone tissue around defects and fused completely at 3-6 months, which indicated the bone defect was almost repaired; At 6-24 months, bone graft was moulded and rebuilt, gradually substituted by newly formed born. CONCLUSION: The CHA is an idea bone graft substitute material for its good results in the treatment of benign osteolytic bone defects, shorter operating time and fewer complications.

AIM: To investigate the influence of Ginkgo biloba extract (EGb761) on c-jun expressions and motoneurons survival following root avulsion. METHODS: One hundred and eighty adult Sprague-Dawley female rats were randomly divided into control and EGb761 groups. Immediately after avulsion of C5-T1 nerve roots, the rats were injected ip with either 1 mL of EGb761 25 mg?kg~(-1)?d~(-1) or the same volume of normal saline, and the treatment repeated everyday. At 4 h to 6 weeks following avulsion, the C7 spinal segments of all rats were collected and prepared for c-jun immunocytochemistry and neutral red stain. The numbers of (c-jun) positive and survival motoneurons were counted and compared between two groups at each time point. RESULTS: In control rats following avulsion, c-jun positive motoneurons appeared at 4 h, reached its maximum at 1 d and declined to 2 weeks. Avulsion-induced motoneurons death started at 2 weeks, climbed to its maximum at 4 weeks-6 weeks. In EGb761 treated rats, both numbers of c-jun positive and survival motoneurons were more than that in control group at each time point. CONCLUSION: EGb761 attenuates avulsion-induced motoneurons death, and this effect may be related to up-regulation of c-jun gene in avulsed motoneurons. [