A study was made; of the effect of malotilate on the acute liver injury induced by carbon tetrachlorid,e ( CC14 ) and d-galactosamine in mice. Malotilate ( 50~150mg/kg ig?3 ) significantly inhibited the elevation of serum glu tamic pyruvic transaminase ( SGPT ) in CC14- intoxicated mice.At the dose of 100mg/kg ig?3, malotilate remarkably increased the content of hepatic glycogea in CCl4-injected mice. The contents of serum protein, liver protein, and cytochrom P-450 in liver hemogenate were increased by malotilate ( 100mg/kg ig?3) in CC14-intoxicated mice. The drug also reduced the accumulation of liver triglycerides induced by CCl4 in mice.In addition to, malotilate(50mg /kg, ip?5) could act against the increase of SGPT and the decrease of liver protein content induced with d-galactosamine in mice. These results suggest that malotilate may be a new therapeutic agent for liver injury.

Armillariella mellea polysaccharide ( AP ) was isolated from solid fermented Armillariella mellea. AP ( 100 mg/kg?d, ig ? 5 d ) increased the production of serum hemolysin in normal mice as well as in im-munodepressed mice induced by cyclophosphamide. At the dose of 50 mg/kg?d ig?5d, AP also caused a significant increase of spleen plaque forming cells ( PFC ) in normal mice. AP ( 10, 50?g/ml ) markedly enhanced Con A-induced lymphocyte proliferation of mouse spleen cells in vitro, but it had no potentiating effect on delayed cutaneous hypersensitivity ( DCH ) to 2,4-dinitrochlorobenzene ( DNCB ) in normal mice. AP ( 100 mg/kg/d, sc? 7d) increased both clearance rate of iv charcoal particles and phagocytic activity of ma-crophages of abdominal cavity in normal mice.

Isopinosine (ISO, 25?100 mg/kg?d-1ip for 4 or 5d) significantly increased the production of serum hemolysin-and spleen plaque forming cell ( PFC) in normal mice immunized with SRBC. The same result was observed in immunosuppressive mice induced by cy clophospha-mide. In 16 month aged mice, ISO (ip for 5 d) showed potentiating effect on anti-SRBC PFC response at the dose of 2 .5 mg/kg, no significant effect at the dose of 5 .0 mg/kg, and inhibiting effect at the dose of 25 and 100 mg/kg respectively. 1SO(25,50 and 100 mg/kg?d-1 ip for 9 or 10 d ) remarkably enhanced delayed cutaneous hypersensiti-vity (DCH) to 2, 4 -dinitrochlorobenzene (DNCB) in normal mice as well as in immunosuppressive mice induced by cyclophospha-mide. ISO (5, 10 and 20 mg/L ) signficantly increased ConA-induced lymphocyte proliferation of spleen cells from C57BL/6J mice in vitro. There was also potentiating effect on lymphocyte proliferation induced by ConA in 16 month aged mice.

Objective:To explore the rules of acupoint sticking therapy based on data mining technology for the treatment of angina pectoris of coronary heart diseases, and to provide a reference for guiding acupoint selection and rational use of Chinese medicine.Methods:To search for the clinical research literature of acupoints for the treatment of angina pectoris of coronary heart diseases from China Academic Journal Database (Wanfang Data), China National Knowledge Resources Database (CNKI), China biomedical literature service system (SinoMed) and Chinese science and technology journal database (Chongqing VIP) dated from January 31, 2020. Using Microsoft Excel 2016, SPSS Statistics 21.0, and SPSS Modeler 14.2 statistical software as tools to conduct association analysis and cluster analysis on those acupoints and Chinese medicine.Results:A total of 102 acupoints application prescriptions were included, involving 38 acupoints. The top 5 frequently used acupoints were Danzhong (RN17), Neiguan (PC6), Xinshu (BL15), Zusanli (ST36), and Zhiyang (DU9). The top 5 acupuncture were Neiguan (PC6)- Sanyinjiao (SP6), Xinshu (BL15)- Geshu (BL17), Danzhong (RN17)- Geshu (BL17), Danzhong (RN17)- Jueyinshu (BL14), Danzhong (RN17)- Geshu (BL17) and Xinshu (BL15). There are 102 prescriptions of applied medicines with 88 kinds of Chinese medicine. The top 5 frequently used Chinese medicines were Chuanxiong Rhizoma, Borneolum Syntheticum, Salviae Miltiorrhizae Radix et Rhizoma, Olibanum, Santali Albi Lignum, the top three drug pairs were Chuanxiong Rhizoma- Cinnamomi Cortex, Allii Macrostemonis Bulbus- Trichosanthis Fructus, Olibanum- Myrrha. Conclusion:The commonly used single points to treat angina pectoris of coronary heart disease are Danzhong, and usually combined with Neiguan and Xinshu, and the acupoints along the Urinary Bladder Meridian of Foot- Taiyang are used frequently; the Chinese medicines are mainly used for activating blood circulation, genenrally reflects the therapeutic principle of removing blood stasis, dredging collaterals and relieving pain.

AIM: To explore the expressive profile of nestin protein in the focal ischemic brain and to study the recovery mechanism of brain focal infarct . METHODS: Cellular morphology, time-course and distribution pattern of nestin positive response were immunohistochemically examined in different brain regions of 36 adult male SD rats. RESULTS: Nestin positive response of different brain regions in sham operated rats was present in small- and micro-vasculartures and the third ventricle bottom and ependyma. A large number of nestin positive cells were detected in ischemic brain, and were more remarkable in the cortical areas of parietal lobe and preoptic area as well as ischemic caudoputamen. Stellate nestin positive cells were located in the deep layer of ischemic cortex, but fibrillary cells were located in the shallow layer. Nestin positive cells in the ischemic caudoputamen showed the same changes of morphology as those cells in the deep layer of ischemic cortex. Morphological and number alterations of nestin positive cells were the most remarkable at 1 weeks post-ischemia, which showed more hypertrophy and proliferation in morphology, and a marked increase in number was present in the ischemic cerebral cortex and the ischemic caudoputamen. These alterations of nestin positive cells persisted up to 6 weeks post-ischemia, and then, the nestin positive response in the ischemic brain decreased gradually. CONCLUSION: Focal cerebral ischemia induces nestin re-expression on reactive astrocytes, which may be very important to the self-recovery of cerebral infarct.

Objective To survey the compliance degree of patients with allergic rhinitis completing their specific desensitizing therapy and to evaluate the relating factors with this compliance degree, and to explore the nursing countermeasures. Methods A retrospective study was carried out in 186 patients with allergic rhinitis receiving specific immunotherapy from January 2007 to December 2008. Noncompliance patients were contacted with scheduled telephone interviews to investigate the causes. Results Compliance rate was 71.0%,primary reason for non-compliance is poor efficacy (16 patients, 29.6% ), followed by busy work or study (10 patients, 18.5%) and disappearance of symptoms (7 patiens, 13.0%). Conclusions The compliance degree of AR patients receiving specific desensitizing therapy is still poor, but the reasons for non-compliance can be controlled by many factors, compliance is still much room for improvement.

Objective To observe the clinical features of migraine based on out-patient clinic data and provide help for the diagnosis and treatment of migraine. Methods In a retrospective study of 309 patients with migraine, we investigated the clinical characteristics of migraine of both genders and different types, and the risk factors for MOH transformed from migraine. Results The female to male ratio was about 3:1,76.1% of the patients had triggering factors. The most common characteristics of headache were moderate to severe intensity of the pain (97.7%), aggravation by routine physical activity (75.1%), and association with nausea (90.9%) and/or vomiting (70.6%). There were significant differences in some clinical characteristics of migraine in females as compared with these in males and in patients with migraine without aura (MWOA) as compared with those with aura (MWA). The risk factors for MOH transformed from migraine were elder age of onset, high attack frequency and the analgesics frequently used (P < 0.05).Conclusion It is suggested that carefully collecting the characteristics of headache, triggering factors and therapeutic history is the foundation of correct diagnosis and effective treatment for migraine.

Objective To investigate the changing characteristics of the fractional anisotropy (FA) in cerebral peduncles and its relation with motor evoked potential (MEP) after acute cerebral infarction and to clear the clinical sigiificance of the low limit value of the FA in cerebral peduncles. Methods The low limit value of the FA in normal cerebral peduncles was determined based on mean - 1. 64 standard deviation. The patients with acute cerebral infarction (n = 58) were divided into MEP positive group and MEP negative group according to the absence and presence of MEP, in which the patients in the MEP positive group were redivided into the FA in cerebral peduncles ＜ the low limit value and≥ the low limit value groups according to the FA in cerebral peduncles on the affected sides. Results The low limit value of the FA in normal cerebral peduncles was 0. 36. There was significant difference in the FA in cerebral peduncles on the affected sides between the MEP negtive and MEP positive groups. The MEP negative group was the lowest (P=0. 000). The FA in cerebral peduncles on the affected sides in the positive group was significantly lower than that on the unaffected sides (P=0. 000), and the latency on the affected sides was longer than that on the normal sides (P=0. 000). The FA in cerebral peduncles on the affected sides was negatively correlated with the MEP latency (r=-0.332,P=0. 042). The MEP latency in the FA＜the low limit value group was significantly longer than that in the FA ≥ low limit value group (P=0. 002). There were no significant differences in the FA in cerebral peduncles on the normal sides and the MEP latency among an groups. The detection rate of the FA in cerebral peduncle＜0. 36 on the affected sides was the highest (50%). Conclusions In the evaluation of the prognosis of the patients, the changes of the FA in cerebral peduncles on the affected sides in patients with acute cerebral infarction had correlation,consistency, and complementarity with MEP.The detection rate of the low limit value of the FA in normal cerebral peduncles was the highest in the MEP negative patients. When the FA in cerebral peduncles was＜0.36 on the affected sides, particularly when MET was negative, it might predict that the prognosis was poor.

Objective: To explore the association of the single nucleotide polymorphisms (SNPs) in N-formylpeptide receptor (FPR) gene with the susceptibility of aggressive periodontitis (AgP). Methods: A total of 94 AgP patients and 73 healthy controls were entered into the study. Peripheral blood sample was obtained from each subject by venepuncture. Genomic DNA was isolated from each sample.The target fragment of FPR gene was amplified by PCR. The SNPs in FPR gene were detected by denature high performance liquid chromatography ( DHPLC) combined with DNA sequencing. Results: There were two non-synonymous SNPs in the 370 bp FPR gene fragment;289C/A and 301G/C. The 289C/Awas a novel SNP. No variation in nucleotides 329 and 378 was detected. There were no statistically significant differences in distributions of the genotypes and alleles for FPR289 and FPR301 between AgP patients and healthy controls. Using multivariate logistic regression (adjusted for age and gender) , it was showed that the adjusted Ors of AgP for the C~+ genotype and allele C of FPR301 combined with smoking were 5.74 and 5.20 respectively. Conclusion: The presence of the C~+ genotype/allele C of FPR301 together with smoking conferred a higher risk for AgP. The result suggests that the SNPs in FPR gene may not be associated with the susceptibility of AgP in Chinese.

After the antidepressant patents were retrieved from Innography, their distribution, R& D level, hot spots and core patents were analyzed , which showed that the largest number of antidepressant patents was produced in USA, the medicinal preparations containing organic active ingredients were the hot spots, the technological and economic strengths of Pfizer, Novo and Sanofi-Aventis were relatively tremendous.The law-suited patents, reex-amined patents and patent strength were mined.