70%) were group R. The distal LAD flow signal could be observed in all cases in group N and 6 cases in group R. The PVd, PVs and DSVR had no significant difference between group N and the controls. The PVd and DSVR were significantly lower in patients of group R than those in patients of group N and the controls. Conclusions Monitoring of the distal LAD flow velocity by TTCDE was useful in the noninvasive assessment of re-stenosis after stent intervention for the LAD lesions.

Objective To investigate the clinical value of mitral valve quantitative(MVQ)soft ware,select the significant parameters in diagnosing mitral valve prolapse and analysis the mechanism of mitral valve regurgitation by MVQ in clinical.Methods Live three dimensional transesophageal echocardiography was performed on thirty-one patients,in which 13 patients with mitral valve prolapse,another 18 patients with suspected intracardiac thrombosis without mitral valve regurgitation regarded as control group.The images were stored and processed with MVQ soft ware off line.Eighteen parameters of mitral valve structure were measured in mitral valve 3D mode.Results Comparing with the control group,the parameters of mitral valve annulus of mitral valve prolapse were larger significantly(P＜0.05).Seven parameters of mitral valve leaflets,θant,θpost,θNPA,Htent,Vtent,L2DALPm,θAv-Mv,had no significant difference between tWO groups(P＞0.05).L3Dant,L3Dpost,Hprol,Vprol,A3Dpost,A3Dant of mitral valve prolapse were larger than those of control group(P＜0.05).Conclusions MVQ can measure the parameters of mitral valve stereo structure quantitatively,result in more detailed diagnosis of mitral valve disease by echocardiography,provide more important information to surgeons,and help them to formulate detailed and complete operation scheme.

Objective To evaluate the accuracy of coronary flow velocity reserve(CFVR) measured by color Doppler coronary flow imaging(CFI) in the patients with myocardial bridging(MB) of the left anterior descending (LAD) coronary artery and to analyze coronary hemodynamic changes and clinical significance of the LAD MB. Methods Consecutive 110 patients with suspected or known coronary disease were examinated by coronary angiography(CA), coronary intravascular ultrasound(IVUS) and intracoronary Doppler(ICD). Of these patients, 11 cases were diagnosed as MB of LAD. The LAD flow were measured by CFI at baseline and hyperemic conditions (intravenous administration of adenosine 140 μg·kg-·min-)and CFVR were calculated. Compared with the measurements of ICD, the accuracy of CFI was analyzed. Nine normal cases served as control group. Results The prevalence of MB was 10% in this study. Seven eases presented with stable angina. MB located in the middle segment of LAD in 7 cases and in the middle-distal segment of LAD in 2 cases. The ratio of LAD stenosis was greater than 80% in 2 cases, one of them simultaneously combined with circumflex stenosis(90 %). CA showed no significant stenosis in 9 cases (MB group) in the LAD and IVUS showed no plaque. Flow velocities were measured by CFI in the middle-distal LAD in nine patients without significant stenosis. CFVR calculated by average peak velocity well correlated with those measured by ICD (regression equation: Y=0.87 X+0.39; correlation coefficient: r=0.81,P<0.01). The coronary flow velocities in hyperemic conditions were significantly lower in MB group than in control group. CFVR were also significantly lower in MB group than in control group. Conclusions CFI is an accurate method for assessment of CFVR. Decreased CFVR of patients with MB may be one of the causes of angina pectoris.

OBJECTIVE:To evaluate the application of acid inhibitors in our hospital and to provide reference for rational use of drugs in the clinic. METHODS:The consumption sum and DDDs of acid inhibitors in our hospital from 2005 to 2009 were statistically analyzed with the method of DDD analysis. RESULTS:Consumption sum of acid inhibitors in our hospital increased year by year,especially for proton pump inhibitor. Consumption sum of H2 receptor antagonist decreased year by year. Omeprazole and pantoprazole took up the top place of DDDs order. Rank ratio of pantoprazole had been kept at 1 for five years with sound synchrony. DUI of these drug were no more than 1,which meant rational drug use. CONCLUSION:The acid inhibitors in our hospital are applied rationally and safe and highly effective proton pump inhibitor is the best choice in the clinic.

Objective: To evaluate the cytotoxicity of nano-hydroxyapatite. Methods: Mouse lung fibroblast L-929 and primary cultured human periodontal ligament fibroblast-like cells (PDLF) were cultured in the medium of extraction of nano-hydroxyapatite or commerce hydroxyapatite at 100%,50%,10% and 0% (control) respectively.Cell growth was tested by MTT assay and the cell relative growth rate (RGR) was calculated.The cytotoxicity was graded by generally accepted standard.Results: RGR of both cell lines cultured in nano-hydroxyapatite extraction was higher than that in control HA. In 7-day culture the cytotoxicity grade of HA was 1 in 100% of HA extraction medium and 0 in 100% of nano-hydroxyapatite extraction,or in 50% and 10% of nano-hydroxyapatite or control HA extraction medium.Primarily cultured PDLF showed lower RGR than L-929 under the same condition. Conclusion: Nano-HA do not have cytotoxic effect.

The left to right shunt rate(F)and the transverse seetional area of ductus arteriosus(A)were calculated by color Doppler flow convergence method in 20 patients with patent ductus arteriosus.The results indicated that the shunt rate(F)was correlated fairly with Qp-Qs,Qp/Qs(measured by pulsed wave Doppler),the pressure gradient(△P)and the diameter of left ventricle(DLV)(r=0.81,0.62,0.69 and 0.53;P＜0.001,0.005,0.001 and 0.01,respectively).The transverse sectional area of ductus arteriosus(A)was also correlated closely with the diameter of ductus arteriosus(Dd)measured directly in two-dimensional view(r=0.81,P＜0.001).The F value of group Qp/Qs≥2:1 was apparently higher than that of group Qp/Qs＜2:1(P＜0.005).The color Doppler flow convergence method can rapidly and exactly evaluate the left to right shunt severity of patent ductus arteriosus.

The shape of regurgitant jets in 30 patients with eccentric mitral regurgitaltoll Showing flow convergence region(FCR)was observed.It was found that the shapes of the jets were allslender and close to left atrial wall.In comparison of the regurgitant volume calculated by pulsed Doppler and regurgitant flow rate by flow convergence with the regurgitant jet area and the ratio 0f the regurgitant area to left atrial area respectively,the results showed that there were poor correlations between them (r=0.59,0.56 and r=0.55,0.57)and good correlation between regurgitant volume determined by Pulsed Doppler and regurgitant flow rate by flow convergence(r=0. 96,P<0.01).When the regurgitant flow rates calculated by flow convergence were same,the regurgitartt jet area and the ratio of the regurgitant jet area to left atrial area of non-eccentric mitral regurgitation were obviously larger than those of eccentric mitral regurgitation.The results indicated that the regurgitant jet area and the ratio of the regurgitant jet area to left atrial area underestimated the severity of eccentric mitral regurgitation apparently and that flow convergence region could be used to assess the seVerItY 0f eccentric mitral regurgitation accurately.

BACKGROUND:It is a key to choose an appropriate method to trans-differentiated bone marrow mesenchymal stem cells (BMSCs)into neuron-like cells for clinical therapy of neural system injury.OBJECTIVE:To observe the feasibility of the differentiation of rat BMSCs supplemented with rat dentate gyrus extract(DGE)into neuron-like cells.METHODS:DGE was applied to induce rat BMSC trans-differentiation,using 20,40,60,80 mg/L protein concentration.Neuron differentiation was measured using Western blot to screen an optimal dosage.After trans-differentiation,different concentration treated cells were collected.Morphology change was observed following differentiation under an inverted microscope.Neuron specific enolase(NSE)and NeuN expression was determined using Western blot.NeuN expression in cells was detected using immunofluorescence technique.RESULTS AND CONCLUSION:After 7 days,60 mg/L DGE was the optimal induction dose detected by Western blot.With increased concentration,NSE and NeuN expression was increased.At 80 mg/L mass concentration,NSE and NeuN expression was reduced.At 60 mg/L DGE,BMSCs following induction became long,with synapses,Immunofluorescence NeuN staining found that neuron-like cells were positive for NeuN following induction.Results indicated that DGE is an effective biological inductor that can induce BMSC differentiation.

Objective To assess global and regional cardiac twist function in hypertrophic cardiomyopathy(HCM) patients by velocity vector imaging(VVl).Methods Thirty HCM patients and 33 normal subjects were enrolled into this study.Two dimensional echocardiographic images of parasternal left ventricular basal level,middle and apical level short axis plane,apical four-and two-chamber plane were obtained.Maximal rotation degree,peak rotation rate,circumferential strain (CS) and strain rate (CSR),radial strain(RS) and strain rate(RSR) in systole were measured using VVI offline software.In addition,diastolic peak un-rotation rate,untwisting rate(unTwR) were measured.Each parameters was analyzed both in subendocardial and subepicardial layer.Results There was no significant difference in left ventricular ejection fraction between two groups.EDV,ESV,SV were significantly decreased (P＜0.01) in HCM group.Left ventricular twist(LVtw),CS of subendocardial layer were higher in HCM group than control group(P＜0.05),while the peak rotation rate,un-rotation rate,CS,CSR were lower of subepicardial layer (P＜0.05).The RSR of each short axis level were significantly decreased in HCM group (P＜0.01).The diastolic unTwR was decreased in HCM group (P＜0.05).For further analysis of the two subgroups in HCM,more regional torsion deterioration occurred in patients with left ventricular outflow tract obstruction than those without obstruction.Conclusions The global cardiac twist angle of HCM was higher than normal person,but the circumferential deformation ability of regional myocardium was impaired,left ventricular outflow tract obstruction may aggravate the regional myocardial dysfunction.