1.Effect of different concentrations of sevoflurane pretreatment on acute lung injury induced by endotoxin in rats
Shuangping ZHAO ; Jiao WU ; Qulian GUO ; Zhong ZHANG ; Zhi YE
Journal of Central South University(Medical Sciences) 2010;35(9):921-927
Objective To investigate the effect of 3 concentrations of sevoflurane pretreatment on acute lung injury induced by endotoxin in rats. Methods Thirty-six male SD rats were randomly divided into 6 groups:a control group(Group A), a sevoflurane group(Group B), a 3.6% sevoflurane pretreatment group(Group C), a 2.4% Sevoflurane pretreatment group(Group D), a 1.2% sevo sevoflurane pretreatment group(Group E), and lipopolysaccharide (Group F).The rats were killed 6 h after lipopolysaccharide (LPS) or saline injection.Histological examinations of the lung tissues were performed with light microscope. Lung wet/dry weight (W/D) ratio, myeloperoxidase (MPO) activity, and intercellular adhesion molecule 1 (ICAM-1) mRNA expression were assayed. Results Introvenous LPS significantly aggravated lung tissue damage,increased lung W/D ratio, MPO activity, and lung ICAM-1 mRNA expression compared with the control group(P<0.05). Precondition with 2.4% sevoflurane significantly attenuated the above mentioned changes induced by LPS (P<0.05). The 3.6% LPS (Group C) significantly attenuated lung tissue damage and decreased MPO activity,lung ICAM-1 mRNA expression compared with the Group F (P<0.05),but no significant change in lung W/D ratio was seen (P>0.05). MPO activity was significantly decreased in Group E (P<0.05), but lung W/D ratio and lung ICAM- 1 mRNA expression had no significant changes (P>0.05).Conclusion Precondition with 2.4% sevoflurane can reduce LPS induced lung injury in rats. Decreased expression of ICAM-1 and less accumulation of neutrophils were participated in its mechanism.
2.Three-dimensional finite element model of mandibular complete overdenture and its biomechanical characteristics
Haishen ZHI ; Yongteng ZHONG ; Jinyong XU ; Fangyong YE ; Jiawei XIANG
Chinese Journal of Tissue Engineering Research 2009;13(26):5189-5193
This study aimed to construct throe-dimensional finite element model of the mandibular complete overdenturo and to analyze the influence rules for stress distribution under different mandibular shapes. The layer cutting method was employed as a basic tool to deal with different plaster models to gain two-dimensional point data. These data were introduced to the commercial software ANSYS to construct throe-dimensional finite element model of the mandibular complete overdenturo, including nine kinds of typical shapes, such as sharpness, roundness and squareness, and etc. Static loads were imposed on denture so as to accomplish biomechanicel analysis and to study the influence rules of stress distribution for mandibular complete overdenturo under different mandibular shapes. Results revealed that the sequence of stress from high to low was squareness, roundness, sharpness and the other extrapolated, basic and inside. The throe-dimensional finite element model has high simulation accuracy and the results provide an experimental foundation and guidance for clinical work.
3.The change of calcitonin gene-related peptide immunoreactive nerve fibers in acute inflammatory pulpitis in rats
Zhi-Zhong CAO ; Jun LIU ; Zhong-Hua ZHOU ; Hui YE ; Wei-Zhong TANG
Academic Journal of Second Military Medical University 2001;22(5):459-460
Objective:To investigate the changes of calcitonin gene-related peptide immunoreactive (CGRP-IR)nerve fibers in rat dental pulps during acute inflammation. Methods: Rat acute pulpitis model was established by silk thread ligation and the change of CGRP-IR nerve fibers was observed with immunohistochemical method.Results: In radical pulp,the CGRP-IR nerve fibers became denser and more heavily stained;in the coronal pulp,the number of CGRP-IR nerve fibers decreased,but the background staining was heavier. Conclusion: During acute inflammation,the amount of CGRP increases in dental pulps, and is released into the surronding tissue in a large scale in the coronal region.
4.Short-term results of cutting balloon and intravascular brachytherapy for the treatment of coronary in-stent restenosis
Fei YE ; Shao-Liang CHEN ; Bao-Xiang DUAN ; Jin HUANG ; Zhi-Zhong LIU ; Jie SONG ;
Chinese Journal of Clinical Pharmacology and Therapeutics 1999;0(04):-
0.05); the levels of LL, LI, RRS in CBA group and CBA+IBT group were significantly lower than those in control group(P
5.Relevant factors on the degree of anterior uveitis in patients with ankylosing spondylitis
Guo-Xiang, SONG ; Jin-Xian, HUANG ; Ya-Ling, DENG ; Zhi-Hua, YIN ; Zhang-Yi, LIANG ; Zhi-Zhong, YE
International Eye Science 2014;(7):1310-1312
AlM: To investigate the association between the degree of anterioruveitis and related factors including inflammatory markers as well as sacroiliac joint imaging in patients with ankylosing spondylitis ( AS) .
METHODS: Anterior changes evaluated by slit lamp, erythrocyte sedimentation rate ( ESR ) , C - reactive protein ( CRP ) and magnetic resonance imaging of 55 cases with AS associateduveitis were retrospectively analyzed. A modified endotoxin-induced uveitis ( ElU ) clinical standard was used for uveitis grading. SPARCC sacroiliac scoring was used to evaluate bone edema of sacroiliac joint. The correlation between the degree of uveitis and sacroiliitis was assessed.
RESULTS: ln the 55 patients with AS, ElU grading scored 2-10, and SPARCC index scored 0-22. Further analysis showed that the severity of uveitis was significantly correlated with ESR (r=0. 869, P<0. 001) and CRP (r=0. 485, P<0. 001). The degree of anterior uveitis in AS patients was not correlated with inflammation of sacroiliac joint (r=0. 237, P=0. 081).
CONCLUSlON: Local autoimmunity of uveitis and sacroiliac joint inflammation with subsequent bone formation in AS might be mutually independent processes.
6.Etanercept combined with Tripterygium wilfordii polyglycoside for treatment of rheumatoid arthritis in the elderly: a clinical study.
Wei-Zhen HE ; Zhi-Hua YIN ; Jian-Hua GAO ; Zhi-Zhong YE ; Yan XIE ; Wei-Hong KONG ; Ya-Shuo CHEN
Chinese Journal of Integrated Traditional and Western Medicine 2014;34(3):267-271
OBJECTIVETo evaluate the efficacy and safety of etanercept plus Tripterygium wilfordii polyglycoside (TWP) in elderly patients with active rheumatoid arthritis (RA).
METHODSTotally 46 elderly patients with active RA were randomly assigned to the treatment group (22 cases) and the control group (24 cases). All patients received subcutaneous injection of etanercept, 25 mg each time, twice per week. The dosage was reduced to once per week 3 months later. Patients in the treatment group took TWP Tablet (10 mg each time, three times per day), while those in the control group took methotrexate (MTX), 10 mg each time, once per week. The whole course lasted for 24 weeks. Patients' rest pain, tender joint number, swollen joint number, health assessment questionnaire (HAQ), patients' global assessment, physicians' global assessment, erythrocyte sediment rate (ESR), C reactive protein (CRP), rheumatic factor were assessed at week 0, 4, 8, 12, and 24. The curative effect was statistically evaluated by the United States Institute of Rheumatology ACR20, ACR50, and ACR70 improvement criteria. Meanwhile, any adverse event was recorded and evaluated.
RESULTSTotally 41 completed the trial, and 5 dropped off (3 in the treatment group and 2 in the control group). Compared with the control group, there was no statistical difference in ACR20, ACR50, or ACR70 in the treatment group (P > 0.05). Compared with before treatment in the same group, there was some improvement in tender joint number, swollen joint number, visual analogue scale (VAS) for patients' global assessment, VAS for physicians' global assessment, ESR, CRP, and HAQ between the two groups, showing statistical difference (P < 0.05). Compared with the control group in the same phase, there was no statistical difference in the treatment group (P > 0.05). There was no statistical difference in the occurrence of adverse events between the two groups.
CONCLUSIONSEtanercept plus TWP could achieve equivalent therapeutic effect to that of Etanercept plus MTX. The two regimens could improve clinical signs, symptoms, and QOL related to RA. They were well tolerated in the treatment of elderly patients with active RA.
Aged ; Antirheumatic Agents ; therapeutic use ; Arthritis, Rheumatoid ; drug therapy ; Drug Therapy, Combination ; Etanercept ; Female ; Glycosides ; therapeutic use ; Humans ; Immunoglobulin G ; therapeutic use ; Male ; Middle Aged ; Receptors, Tumor Necrosis Factor ; therapeutic use ; Treatment Outcome ; Tripterygium ; chemistry
7.Mutations in UGT1A1 gene in neonates with hyperbilirubinemia of Guangxi Heiyi Zhuang nationality.
Xiao-Jing WU ; Dan-Ni ZHONG ; De-Zhi YE ; Yong ZHONG ; Xiang-Zhi XIE
Chinese Journal of Contemporary Pediatrics 2014;16(5):483-488
OBJECTIVETo study the distribution of mutations of UDP-glucuronosyltransferase 1A1 (UGT1A1) gene and its relationship with hyperbilirubinemia among neonates with hyperbilirubinemia of Guangxi Heiyi Zhuang nationality.
METHODSTotal genomic DNA was extracted from the blood of 100 neonates with hyperbilirubinemia (case group) and 100 neonates without hyperbilirubinemia (control group), all of whom were selected from Guangxi Heiyi Zhuang population. TATA box and all exons of UGT1A1 gene were amplified by PCR and directly sequenced.
RESULTS(TA)7 insertion mutation in TATA box, G71R missense mutation in exon 1, and 4 single nucleotide polymorphisms (SNPs) (rs199539868, rs114982090, rs1042640 and rs8330) in exon 5 were observed. The allele frequency of G71R mutation in the case group was significantly higher than that in the control group (P<0.01). There were no significant differences in the genotype distribution and allele frequency of TATA box mutation and SNPs (rs1042640 and rs8330) between the two groups (P>0.05). The logistic regression analysis showed that the odds ratios (95% confidence intervals) of UGT1A1 TATA box mutation, G71R mutation, and SNPs (rs1042640 and rs8330) associated with the development of neonatal hyperbilirubinemia were 0.846 (0.440, 1.629), 3.932 (1.745, 8.858), 0.899 (0.364, 2.222), respectively.
CONCLUSIONS(TA)7 insertion mutation and G71R missense mutation of UGT1A1 gene are common mutation types in neonates with hyperbilirubinemia of Guangxi Heiyi Zhuang nationality. Four SNPs (rs199539868, rs114982090, rs1042640, and rs8330) was first reported in China. UGT1A1 G71R missense mutation is a risk factor for hyperbilirubinemia in neonates of Guangxi Heiyi Zhuang nationality.
China ; ethnology ; Glucuronosyltransferase ; genetics ; Humans ; Hyperbilirubinemia, Neonatal ; genetics ; Infant, Newborn ; Logistic Models ; Mutation ; Polymorphism, Single Nucleotide ; TATA Box
8.The effect of intermittent deferoxamine therapy on relieving iron overload in children with ? thalassemia
bo, WANG ; yong-hong, LAI ; guo-yu, ZHONG ; rong-jie, YU ; tie-zhen, YE ; fu-xiong, CHEN ; zhi-liang, WU
Journal of Applied Clinical Pediatrics 1986;0(01):-
Objective To evaluate the effect of the intermittent deferomamine(DF) therapy on relieving iron overload caused by transfusion in children with ? thalassemia.Methods Sixteen children who were finally diagnosed as ? thalassemia major were treated with deferomamine for 124 times totally to low the iron overload. The serum iron(SI), serum ferritin(SF) and urine ferritin were detected each time with radio-immunity technique and difference was compared before and after treatment. Meanwhile, weather DF involved children′s liver and renal function was observed in whole procedure.Results Iron overload exists in 16 cases of ? thalassemia major children by a long- term hypertransfusion therapy, with average level SI 33.69?6.72 mmol/L,SF 441.19? 54.70 ?g/L,urine ferritin 8.64?6.79 ?g/L. The difference was significant (paired-samples t test,t =6.173 P 0.05).Conclusion The study suggest that intermittent low-dose DF therapy is effective for iron overload caused by transfusion in ? thalassemia children, without apparent side effects.
9.Cloning and expression analysis of pathogenesis-related protein 1 gene of Panax notoginseng.
Rui-Bo LI ; Xiu-Ming CUI ; Yu-Zhong LIU ; Zhi-Gang WU ; Shu-Fang LIN ; Ye SHEN ; Lu-Qi HUANG
Acta Pharmaceutica Sinica 2014;49(1):124-130
By reverse transcription-polymerase chain reaction (RT-PCR), an open reading frame of pathogenesis-related protein 1 (PR1) was isolated from Panax notoginseng and named as PnPR1. Molecular and bioinformatic analyses of PnPR1 revealed that an open reading frame of 501 bp was predicted to encode a 166-amino acid protein with a deduced molecular mass of 18.1 kD. Homology analysis showed that the deduced amino acid sequence of PR1 protein of Panax notoginseng had a high similarity with other higher plants had the same conservative structure domain of cysteine-rich secretory protein (CAP). The recombinant expressed plasmid pET28a(+)-PnPR1 was expressed in Escherichia coli BL21. The expression conditions were optimized by induction at different times, different temperatures, different IPTG concentrations and different giving times. The optimum expression condition was 0.4 mmol.L-1 IPTG at 28 degrees C for 20 h. The successful expression of PnPR1 provides some basis for protein purification and preparation of the monoclonal antibody.
Amino Acid Sequence
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Cloning, Molecular
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Escherichia coli
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metabolism
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Molecular Weight
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Open Reading Frames
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genetics
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Panax notoginseng
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chemistry
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Phylogeny
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Plant Proteins
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genetics
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metabolism
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Plants, Medicinal
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chemistry
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Reverse Transcriptase Polymerase Chain Reaction
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Sequence Alignment
10.Inflatable hollow obturator prostheses for patients undergoing an extensive maxillectomy: a case report.
Yue-Zhong HOU ; Zhi HUANG ; Hong-Qiang YE ; Yong-Sheng ZHOU
International Journal of Oral Science 2012;4(2):114-118
The presence of a large palatal or maxillary defect after partial or total maxillectomy for tumor, trauma or congenital deformation poses a challenge to prosthodontists, particularly when the use of an implant cannot be considered. This case report described the use of an air valve in a hollow silicone obturator to manufacture an inflatable obturator that could be extended further into undercut area to retain itself. The inflatable obturator exhibited adequate retention, stability and border sealing, thereby improving the masticatory,pronunciation and swallowing functions of patients. It may be a suitable alternative treatment option to an implant-retained obturator.
Dental Impression Technique
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Dental Prosthesis Design
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Dental Prosthesis Retention
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Denture Retention
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Denture, Complete, Upper
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Fibrous Dysplasia of Bone
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rehabilitation
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surgery
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Humans
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Male
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Maxilla
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surgery
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Orthognathic Surgical Procedures
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instrumentation
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methods
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rehabilitation
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Palatal Obturators
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Silicones
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Treatment Outcome
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Young Adult